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General Information
Symbol
Dmel\hid05014
Species
D. melanogaster
Name
FlyBase ID
FBal0009522
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
hid-lacZ, l(3)05014, hidP05014, W05014, hidP, hidl(3)05014, P{PZ}W05014
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{PZ} insertion within the open reading frame between amino acids 105 and 106.

Insertion components
P{PZ}hid05014
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous and W05014/Df(3L)X14 animals do not show a defect in the programmed cell death of vCrz neurons: the neurons are no longer present at 7 hours after puparium formation, as occurs in wild-type.

As in wild type, W05014/Df(3L)X14 mutant bursCCAP neurons undergo programmed cell death shortly after eclosion.

The programmed cell death of bursCCAP neurons seen in the adult ventral nerve cord of wild type flies after eclosion is suppressed to a similar extent in W05014/Df(3L)H99 and Df(3L)H99/+ mutant flies.

Wild type larval wing discs show two phases of apoptosis in response to irradiation: first signs of cell death are clear after 4h and plateau, with cell death again increasing from 16-24h; irradiated hid05014 discs show significantly less apoptosis at 4h compared to wild type, and show a similar second phases of induction at 16-24h but at significantly lower levels.

In contrast to wild-type controls, DNA-damage induced apoptosis is completely abolished in homozygous W05014 mutant eye discs.

Mutants show defects in destruction of the larval salivary glands, having persistant larval salivary glands at the pupal stage.

W05014/Df(3L)H99 males have a rotated genitalia defect. 100% of W05014/Df(3L)H99 flies have a ballooned wing phenotype.

W05014/Df(3L)H99 animals have extra interommatidial cells in the pupal eye disc.

Most W05014/Df(3L)H99 (or Df(3L)X14) mutants are embryonic lethal, with only a few escapers.

W05014/Df(3L)H99 and W05014/Df(3L)X14 mutants exhibit normal levels of programmed cell death in Crz-expressing neurons of the ventral nerve cord.

The wing discs of W05014/+ mutant third instar larvae exhibit reduced levels of apoptosis in response to X-ray induced irradiation with 4000 rads compared to controls.

W05014/WrvX1 pupal eye discs lack any evidence of "early stage" cell death (this normally occurs between 18-24 hours after puparium formation). The ommatidia in these pupal retinas are disorganised compared to wild type due to the excess of cells present. The retinas often appear to be attached to what seems to be the antennal disc in the pupae.

W05014/Df(3L)H99 mutant animals exhibit ectopic branching on their aristae.

cysts from W05014/Df(3L)H99 mutant male testes, show defects in spermatid individualisation.

The level of photoreceptor apoptosis at the periphery of developing eyes at 42 hours after puparium formation is reduced, but not eliminated, in W05014 homozygous clones.

Homozygous embryos have defects in head involution but their segment polarity is normal.

Pronounced defect in the morphogenetic movements of head involution which results, in part, from a failure of the dorsal fold to migrate anteriorly. Otherwise the segmented cuticle is normal and individuals reach advanced stages. There is less apoptosis when compared to wild type, most noticeably in the head region prior to completion of head involution.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement
Reference

hid05014 has decreased cell death | third instar larval stage | conditional phenotype, non-enhanceable by Clbn1Q/Clbn1Q

hid05014 has decreased cell death | recessive phenotype, non-enhanceable by Rbf120a

Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

hid05014 has decreased cell death | third instar larval stage | conditional phenotype, non-suppressible by Clbn1Q/Clbn1Q

hid05014 has decreased cell death | recessive phenotype, non-suppressible by Rbf120a

NOT Enhancer of
Statement
Reference

hid05014 is a non-enhancer of decreased cell death | third instar larval stage | conditional phenotype of Clbn1Q

Suppressor of
Statement
Reference

hid05014 is a suppressor of increased cell death phenotype of Rbf15aΔ, gig64

hid05014 is a suppressor of increased cell death phenotype of Rbf15aΔ, gig192

hid05014/Df(3L)H99 is a suppressor | partially of visible phenotype of Ras85DKP

NOT Suppressor of
Phenotype Manifest In
NOT Enhanced by
Statement
Reference

hid05014 has wing disc | third instar larval stage | conditional phenotype, non-enhanceable by Clbn1Q/Clbn1Q

hid05014 has eye disc phenotype, non-enhanceable by Rbf120a

Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

hid05014 has wing disc | third instar larval stage | conditional phenotype, non-suppressible by Clbn1Q/Clbn1Q

hid05014 has eye disc phenotype, non-suppressible by Rbf120a

NOT Enhancer of
Statement
Reference

hid05014 is a non-enhancer of wing disc | third instar larval stage | conditional phenotype of Clbn1Q

Suppressor of
Statement
Reference

hid05014/Df(3L)H99 is a suppressor | partially of eye phenotype of Ras85DKP

hid05014 is a suppressor of phenotype of arm4

hid05014/W[+] is a suppressor of embryonic epidermis phenotype of arm3

NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

hid05014 suppresses the severe irradiation-induced germline stem cell loss seen in Df(3L)banΔ1/+ mutants.

A hid05014 mutant background suppresses the eye phenotype seen upon expression of lin-52GD12885 under the control of Scer\GAL4GMR.PF, along with a dramatic reduction in apoptosis in the eye-antennal disc.

hid05014 Clbn1Q/Clbn1Q double mutants show similar apoptosis induction in wing discs in response to irradiation as either mutant alone. hid05014 suppresses the induction of apoptosis seen in third instar eye discs with expression of ClbnScer\UAS.cWa driven by Scer\GAL4GMR.PU.

One copy of W05014 partially suppresses the apoptosis seen in the posterior compartment of third instar larval wing discs expressing E2fPIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E.

One copy of W05014 partially suppresses the apoptosis seen in the posterior compartment of third instar larval wing discs expressing E2fPIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E.

hid05014/+ does not suppress the late-onset retinal degeneration phenotype of ebi1-334.GMR/+ flies.

Removal of one copy of W through a W05014 heterozygous background significantly attenuates nabEP642-induced apoptosis in the dorsal compartment of the wing imaginal disc (using the Scer\GAL4ap.nd432 driver).

W05014/+ partially suppresses the loss of interommatidial bristles which is seen in bftΔ263a/bftunspecified flies.

The presence of a W05014 background significantly decreases gig192/Rbf15aΔ induced cell death. Some cell death is still observed, particularly in the anterior of the eye disc.

The presence of a W05014 background significantly decreases gig64/Rbf15aΔ induced cell death. Some cell death is still observed, particularly in the anterior of the eye disc.

Rbf15aΔ; W05014 double mutant clones occupy a dramatically increased area of the eye compared to Rbf15aΔ single mutant clones. The morphology of the eye is relatively normal with occasional extra interommatidial bristles. No cell death or nuclear pyknosis is observed in eye disc clones.

Df(3L)H99/W05014 partially suppresses the cell death phenotype of Ras85DKP mutant flies.

Expression of hepdsRNA.Scer\UAS (under the control of Scer\GAL4GMR.PF) in flies carrying Df(3L)H99/W05014 in a Ras85DKP mutant background strongly suppresses the eye phenotype.

A W05014 heterozygous background suppresses lethality found in Df(3L)banΔ1 mutants to generate wild-type levels of survival when larvae are irradiated with 0-8000 R of X-rays.

Similarly to W05014 single mutants, DNA-damage induced apoptosis is completely abolished in Rbf120a, W05014 double mutant eye discs.

Removal of zygotic W (W05014/Df(3L)H99) suppresses the pole cell apoptosis seen in embryos derived from nosBN females. Approximately 7% of pole cells examined at stage 12-14 are undergoing apoptosis.

Removal of zygotic W (W05014/Df(3L)H99) suppresses the pole cell apoptosis seen when Tao-1Scer\UAS.P\T.T:Zzzz\FLAG is expressed under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in stage 13-16 embryos derived from nosBN females.

Removal of zygotic W (W05014/Df(3L)H99) suppresses the pole cell apoptosis seen when sklScer\UAS.P\T.cSa is expressed under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in stage 13-16 embryos derived from nosBN females.

Expressing CycEScer\UAS.cLa under the control of Scer\GAL4en-e16E in W05014 embryos reduces the level of apoptosis in embryonic posterior compartment cells by 65% compared to when CycEScer\UAS.cLa is expressed in a wild-type background.

There is no increase in the survival of neuroblasts in the abdominal neuromeres in W05014/Df(3L)H99 larvae compared to wild type.

Addition of W05014 or Df(3L)H99 does not result in any pronounced improvement of the wgl-8 segment polarity defect; all cells still secrete a uniform lawn of denticles and the cuticle remains smaller than wild type in double mutant embryos.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

A. Spradling.

Comments
Comments

Complements: Eip75B00225. Complements: l(3)0071400714. Complements: not02069. Complements: Eip75B03247. Complements: not06806. Complements: l(3)j13B3j13B3. Complements: notj4B8a. Complements: notj9A6. Complements: l(3)neo261.

Precise excision of the P{PZ} insertion demonstrates the P-element insertion is responsible for the mutant phenotype.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (10)
References (44)