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General Information
Symbol
Dmel\InR05545
Species
D. melanogaster
Name
FlyBase ID
FBal0009539
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
InRp5545, l(3)05545, InR5545
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion of a P{PZ} element within the 5' end of the coding sequence.

P{PZ} insertion within the first coding exon.

Insertion components
P{PZ}InR05545
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

A small number of homozygotes reach the third larval instar stage. These larvae show an increase in the duration of this larval stage and develop melanotic masses.

Heterozygous InR05545 mutant flies reared on food containing 7.5% ethanol exhibit reduced adult viability compared to controls. Viability is comparable with controls when flies are raised on conventional media.

InR05545/InR3T5 mutant adult flies have a reduced body weight compared to heterozygous controls. Wing area is also reduced compared to controls.

Lipid content is increased in InR05545/InR3T5 mutant flies compared to controls. Carbohydrate content is similar to controls.

InR05545/InR3T5 mutant flies exhibit normal electroretinogram (ERG) readings.

The frequency of proliferating cells in the intestine of aging InRE19/InR05545 flies is significantly lower than that of age-matched controls.

The average number of germline stem cells (GSCs) in the testis of InRE19/InR05545 males within 24 hours of eclosion is similar to that seen in wild-type males at this time. However, the mutant males show an increased rate of reduction in GSC number with age compared to wild-type males, such that 14 days after eclosion, while the wild-type males maintain 90% of the GSC numbers of newly eclosed males, the mutant males maintain only 75% of the average GSC number of newly eclosed males.

InRE19/InR05545 males that are within 24 hours of eclosion have a reduced number of stage S2b spermatocyte cysts in the testis compared to wild-type males.

The average diameter of stage S6 InRE19/InR05545 spermatocytes is smaller than that of control spermatocytes at this stage.

The severity of cocaine-induced follicle degeneration in InR05545/+ females is indistinguishable from that observed in wild-type females.

Homozygous clones in the eye contain ommatidia which are smaller than normal.

Heterozygous males and females show a moderate reduction in life expectancy compared to controls (from 32.3 to 23.7 and from 35.5 to 30.5 days respectively) and are normal in size. InRE19/InR05545 adults are dwarf in size. Developmental rate is slower than normal. Females show an 85% increase in life expectancy compared to controls (from 32.3 to 60.1 days). Males show a high mortality as early adults, but have reduced mortality at late ages, resulting in a life expectancy at 10 days that is 43% greater than that of controls. InR05545/InRE19 females require continuous exposure to methoprene to induce any vitellogenesis. Treatment with methoprene reduces the longevity of InR05545/InRE19 females towards wild-type levels.

Embryonic CNS defects.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

InR05545/InR353 has lethal phenotype, suppressible by Tsc129

NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

InR05545 is a non-suppressor of small body | pupal stage phenotype of Nf1E2

InR05545 is a non-suppressor of visible | somatic clone phenotype of Tsc1Q600X

Other
Phenotype Manifest In
Enhancer of
Statement
Reference

InR05545 is an enhancer of eye phenotype of chicoflp147E

InR05545 is an enhancer of wing phenotype of chicoflp147E

InR05545 is an enhancer of adult phenotype of chicoflp147E

NOT Enhancer of
Statement
Reference

InR[+]/InR05545 is a non-enhancer of photoreceptor cell phenotype of SIIN

Suppressor of
Statement
Reference

InR05545 is a suppressor | partially of eye | somatic clone phenotype of Pten117

NOT Suppressor of
Statement
Reference

InR[+]/InR05545 is a non-suppressor of photoreceptor cell phenotype of SIIN

InR05545 is a non-suppressor of ommatidium | somatic clone phenotype of Tsc1Q600X

Additional Comments
Genetic Interactions
Statement
Reference

A InR05545 mutant background only slightly reduces the overgrowth found in Pten117 mutant eye clones.

EcRM554fs/+; InR05545/+ females do not show the cocaine-induced degeneration of vitellogenic follicles seen in either single heterozygote or wild-type flies.

Tsc129 suppresses the lethality of InR353/InR05545 animals, allowing 39% animals to survive to adults.

Clones in the eye doubly mutant for InR05545 and Tsc1Q600X show the Tsc1Q600X single mutant phenotype of enlarged ommatidia.

The phenotype of homozygous chicoflp147E flies is enhanced by a copy of InR05545. Overall wing size is reduced by 17.7%, the number of cells reduced by 19.8%. In the eye, the number of ommatidia and size of the eye is also reduced.

The lethality caused by sevS11.T:Hsap\MYC in combination with Ras85DV12.sev is suppressed by InR05545.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

A. Spradling.

Comments
Comments

Complements: mod(mdg4)03852. Complements: E2f07172. Complements: mod(mdg4)j2B7. Complements: E2frM729.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (15)
References (32)