Allele Dmel\InR⁰⁵⁵⁴⁵

General Information
Symbol	Dmel\InR⁰⁵⁵⁴⁵	Species	D. melanogaster
Name		FlyBase ID	FBal0009539
Feature type	allele	Associated gene	Dmel\InR
Also Known As	InR^p5545, l(3)05545

Map ( GBrowse )	Untitled Document
Allele class	hypomorphic allele - genetic evidence
Mutagen	P-element activity
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Fernandez et al., 1995)
Mutagen	P-element activity (Fernandez et al., 1995)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3R:17,405,991..17,405,991 (Fernandez et al., 1995, BDGP Project Members, 1994-1999, Spradling et al., 1999, Maixner et al., 1998, Tatar et al., 2001, Chen et al., 1996)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Insertion of a P{PZ} element within the 5' end of the coding sequence. (Chen et al., 1996) P{PZ} insertion within the first coding exon. (Fernandez et al., 1995)
Caused by insertion	P{PZ}InR⁰⁵⁵⁴⁵ (Fernandez et al., 1995, BDGP Project Members, 1994-1999, Spradling et al., 1999, Maixner et al., 1998, Tatar et al., 2001, Chen et al., 1996)
Cytology
Phenotypic Data
Phenotypic Class
	developmental rate defective (with InR^E19) (Tatar et al., 2001) developmental rate defective \| third instar larval stage (Bolukbasi et al., 2012) female sterile (with InR^E19) (Tatar et al., 2001) lethal (with InR³⁵³) (Gao and Pan, 2001) lethal \| embryonic stage \| recessive (Fernandez et al., 1995) lethal \| recessive (Spradling et al., 1999) long lived (with InR^E19) (Tatar et al., 2001) long lived \| dominant (Tatar et al., 2001) male sterile (with InR^E19) (Ueishi et al., 2009) male sterile \| partially (with InR^E19) (Tatar et al., 2001) melanotic mass phenotype \| third instar larval stage (Bolukbasi et al., 2012) small body (with InR^E19) (Tatar et al., 2001) viable \| male (with InR^E19) (Ueishi et al., 2009) visible \| recessive \| somatic clone (Potter et al., 2001)
Phenotype Manifest In
	adult gut (with InR^E19) (Biteau et al., 2010) commissure (Fernandez et al., 1995) embryonic/larval brain \| embryonic stage (Fernandez et al., 1995) fascicle (Fernandez et al., 1995) longitudinal connective (Fernandez et al., 1995) male germline stem cell (with InR^E19) (Ueishi et al., 2009) melanotic mass (Bolukbasi et al., 2012) ommatidium \| somatic clone (Potter et al., 2001) polar primary spermatocyte (with InR^E19) (Ueishi et al., 2009) primary spermatocyte (with InR^E19) (Ueishi et al., 2009)
Detailed Description
	Statement Reference A small number of homozygotes reach the third larval instar stage. These larvae show an increase in the duration of this larval stage and develop melanotic masses. (Bolukbasi et al., 2012) The frequency of proliferating cells in the intestine of aging InR[E19]/InR[05545] flies is significantly lower than that of age-matched controls. (Biteau et al., 2010) The average number of germline stem cells (GSCs) in the testis of InR[E19]/InR[05545] males within 24 hours of eclosion is similar to that seen in wild-type males at this time. However, the mutant males show an increased rate of reduction in GSC number with age compared to wild-type males, such that 14 days after eclosion, while the wild-type males maintain 90% of the GSC numbers of newly eclosed males, the mutant males maintain only 75% of the average GSC number of newly eclosed males. InR[E19]/InR[05545] males that are within 24 hours of eclosion have a reduced number of stage S2b spermatocyte cysts in the testis compared to wild-type males. The average diameter of stage S6 InR[E19]/InR[05545] spermatocytes is smaller than that of control spermatocytes at this stage. (Ueishi et al., 2009) The severity of cocaine-induced follicle degeneration in InR⁰⁵⁵⁴⁵/+ females is indistinguishable from that observed in wild-type females. (Willard et al., 2006) Homozygous clones in the eye contain ommatidia which are smaller than normal. (Potter et al., 2001) Heterozygous males and females show a moderate reduction in life expectancy compared to controls (from 32.3 to 23.7 and from 35.5 to 30.5 days respectively) and are normal in size. InR^E19/InR⁰⁵⁵⁴⁵ adults are dwarf in size. Developmental rate is slower than normal. Females show an 85% increase in life expectancy compared to controls (from 32.3 to 60.1 days). Males show a high mortality as early adults, but have reduced mortality at late ages, resulting in a life expectancy at 10 days that is 43% greater than that of controls. InR⁰⁵⁵⁴⁵/InR^E19 females require continuous exposure to methoprene to induce any vitellogenesis. Treatment with methoprene reduces the longevity of InR⁰⁵⁵⁴⁵/InR^E19 females towards wild-type levels. (Tatar et al., 2001) Embryonic CNS defects. (Fernandez et al., 1995)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference InR⁰⁵⁵⁴⁵/InR³⁵³ has lethal phenotype, suppressible by Tsc1²⁹ (Gao and Pan, 2001)
Suppressor of
Statement Reference InR⁰⁵⁵⁴⁵ is a suppressor of lethal phenotype of Ras85D^V12.sev, sev^{S11.T:Hsap\MYC} (Maixner et al., 1998)
NOT Suppressor of
Statement Reference InR³⁵/InR⁰⁵⁵⁴⁵ is a non-suppressor of increased cell size \| somatic clone phenotype of Pten^dj189 (Gao et al., 2000) InR⁰⁵⁵⁴⁵ is a non-suppressor of visible \| somatic clone phenotype of Tsc1^Q600X (Potter et al., 2001)
Other
Statement Reference InR⁰⁵⁵⁴⁵, Ras85D^V12.sev, sev^{S11.T:Hsap\MYC} has viable phenotype (Maixner et al., 1998, Maixner et al., 1998) InR⁰⁵⁵⁴⁵/InR³⁵³, Tsc1²⁹ has partially lethal - majority live phenotype (Gao and Pan, 2001, Gao and Pan, 2001)
Phenotype Manifest In
Enhancer of
Statement Reference InR⁰⁵⁵⁴⁵ is an enhancer of adult phenotype of chico^flp147E (Bohni et al., 1999) InR⁰⁵⁵⁴⁵ is an enhancer of eye phenotype of chico^flp147E (Bohni et al., 1999) InR⁰⁵⁵⁴⁵ is an enhancer of wing phenotype of chico^flp147E (Bohni et al., 1999)
NOT Suppressor of
Statement Reference InR³⁵/InR⁰⁵⁵⁴⁵ is a non-suppressor of wing margin bristle \| somatic clone phenotype of Pten^dj189 (Gao et al., 2000) InR⁰⁵⁵⁴⁵ is a non-suppressor of ommatidium \| somatic clone phenotype of Tsc1^Q600X (Potter et al., 2001)
Additional Comments
Genetic Interactions
Statement Reference EcR^M554fs/+; InR⁰⁵⁵⁴⁵/+ females do not show the cocaine-induced degeneration of vitellogenic follicles seen in either single heterozygote or wild-type flies. (Willard et al., 2006) Tsc1²⁹ suppresses the lethality of InR³⁵³/InR⁰⁵⁵⁴⁵ animals, allowing 39% animals to survive to adults. (Gao and Pan, 2001) Clones in the eye doubly mutant for InR⁰⁵⁵⁴⁵ and Tsc1^Q600X show the Tsc1^Q600X single mutant phenotype of enlarged ommatidia. (Potter et al., 2001) The phenotype of homozygous chico^flp147E flies is enhanced by a copy of InR⁰⁵⁵⁴⁵. Overall wing size is reduced by 17.7%, the number of cells reduced by 19.8%. In the eye, the number of ommatidia and size of the eye is also reduced. (Bohni et al., 1999) The lethality caused by sev^{S11.T:Hsap\MYC} in combination with Ras85D^V12.sev is suppressed by InR⁰⁵⁵⁴⁵. (Maixner et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	11661 ry⁵⁰⁶ P{PZ}InR⁰⁵⁵⁴⁵/TM3, ry^RK Sb¹ Ser¹
Notes on Origin
Discoverer	A. Spradling.

Comments
	Complements: mod(mdg4)⁰³⁸⁵². Complements: E2f⁰⁷¹⁷². Complements: mod(mdg4)^j2B7. Complements: E2f^rM729. (BDGP Project Members, 1994-1999)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	5545 (Chen et al., 1996) dinr^P5545 (Potter et al., 2001) dInR^PZ (Rideout et al., 2012) InR(p5545) (Tu et al., 2002) Inr[PZ] (Clancy et al., 2001) InR⁰⁵⁵⁴⁵ (Bolukbasi et al., 2012, Christensen et al., 2008.12.28, Christensen et al., 2008.12.28, Ueishi et al., 2009, Biteau et al., 2010) inr^l(3)05545 (Gao et al., 2000) InR^p5545 (Wolkow, 2002, Aigaki et al., 2002, Tatar et al., 2001) InR^PZ (Fernandez et al., 1995) Insulin-like receptor⁰⁵⁵⁴⁵ (Willard et al., 2006) l(3)05545 (Spradling et al., 1999, Maixner et al., 1998) l(3)05545⁰⁵⁵⁴⁵ lnR⁰⁵⁵⁴⁵ (Okamoto et al., 2013)
Name Synonym
Secondary FlyBase IDs

References ( 23 )

Generate a list of	All references relating to this allele ( 23 )

List References by type	Research paper ( 16 ) Review ( 2 ) Personal communication to FlyBase ( 4 ) Abstract ( 1 )
Recent research papers ( 3 )
	Okamoto et al., 2013, Genes Dev. 27(1): 87--97 A secreted decoy of InR antagonizes insulin/IGF signaling to restrict body growth in Drosophila. [FBrf0220509] Bolukbasi et al., 2012, Open Biol. 2(1): 110031 Drosophila poly suggests a novel role for the Elongator complex in insulin receptor-target of rapamycin signalling. [FBrf0218466] Rideout et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(4): 1139--1144 Drosophila RNA polymerase III repressor Maf1 controls body size and developmental timing by modulating tRNAiMet synthesis and systemic insulin signaling. [FBrf0217359] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...

Allele Dmel\InR05545

Allele Dmel\InR⁰⁵⁵⁴⁵