FlyBase Allele Report: Dmel\InR[05545]

(Kamakura, 2011, Murillo-Maldonado et al., 2011, Tatar et al., 2001, Spradling et al., 1999, Maixner et al., 1998, Chen et al., 1996, Fernandez et al., 1995, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of diabetes mellitus

CEA

(Banzai and Nishimura, 2023)

CEC with InR^Y902C

(Banzai and Nishimura, 2023)

model of type 2 diabetes mellitus

CEA with InR^3T5

(Murillo-Maldonado et al., 2011)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates fragile X syndrome

modeled by Fmr1³

(Monyak et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate (with InR^E19)

(Tatar et al., 2001)

abnormal developmental rate | third instar larval stage

(Bolukbasi et al., 2012)

chemical sensitive | dominant

(McClure et al., 2011)

decreased body size (with InR^E19)

(Tatar et al., 2001)

decreased body size | adult stage (with InR^3T5)

(Murillo-Maldonado et al., 2011)

decreased body size | adult stage (with InR^sP1)

(Neuman and Bashirullah, 2018)

decreased occurrence of cell division | adult stage (with InR^E19)

(Biteau et al., 2010)

female sterile (with InR^E19)

(Tatar et al., 2001)

lethal (with InR³⁵³)

(Gao and Pan, 2001)

lethal | recessive

(Spradling et al., 1999)

lethal - all die during embryonic stage | recessive

(Fernandez et al., 1995)

long lived (with InR^E19)

(Tatar et al., 2001)

long lived | dominant

(Tatar et al., 2001)

male semi-sterile (with InR^E19)

(Tatar et al., 2001)

male sterile (with InR^E19)

(Ueishi et al., 2009)

melanotic mass phenotype | third instar larval stage

(Bolukbasi et al., 2012)

partially lethal - majority die (with InR^sP1)

(Neuman and Bashirullah, 2018)

some die during embryonic stage | recessive

(Fernandez et al., 1995)

viable (with InR^3T5)

(Murillo-Maldonado et al., 2011)

viable | male (with InR^E19)

(Ueishi et al., 2009)

visible | adult stage (with InR^3T5)

(Murillo-Maldonado et al., 2011)

visible | recessive | somatic clone

(Potter et al., 2001)

Phenotype Manifest In

adult gut (with InR^E19)

(Biteau et al., 2010)

fascicle

(Fernandez et al., 1995)

larval brain | embryonic stage

(Fernandez et al., 1995)

larval longitudinal connective

(Fernandez et al., 1995)

male germline stem cell (with InR^E19)

(Ueishi et al., 2009)

melanotic mass

(Bolukbasi et al., 2012)

ommatidium | somatic clone

(Potter et al., 2001)

polar primary spermatocyte (with InR^E19)

(Ueishi et al., 2009)

primary spermatocyte (with InR^E19)

(Ueishi et al., 2009)

symmetrical commissure

(Fernandez et al., 1995)

wing (with InR^3T5)

(Murillo-Maldonado et al., 2011)

Detailed Description

Statement

Reference

A small number of homozygotes reach the third larval instar stage. These larvae show an increase in the duration of this larval stage and develop melanotic masses.

(Bolukbasi et al., 2012)

Heterozygous InR⁰⁵⁵⁴⁵ mutant flies reared on food containing 7.5% ethanol exhibit reduced adult viability compared to controls. Viability is comparable with controls when flies are raised on conventional media.

(McClure et al., 2011)

InR⁰⁵⁵⁴⁵/InR^3T5 mutant adult flies have a reduced body weight compared to heterozygous controls. Wing area is also reduced compared to controls.

Lipid content is increased in InR⁰⁵⁵⁴⁵/InR^3T5 mutant flies compared to controls. Carbohydrate content is similar to controls.

InR⁰⁵⁵⁴⁵/InR^3T5 mutant flies exhibit normal electroretinogram (ERG) readings.

(Murillo-Maldonado et al., 2011)

The frequency of proliferating cells in the intestine of aging InR^E19/InR⁰⁵⁵⁴⁵ flies is significantly lower than that of age-matched controls.

(Biteau et al., 2010)

The average number of germline stem cells (GSCs) in the testis of InR^E19/InR⁰⁵⁵⁴⁵ males within 24 hours of eclosion is similar to that seen in wild-type males at this time. However, the mutant males show an increased rate of reduction in GSC number with age compared to wild-type males, such that 14 days after eclosion, while the wild-type males maintain 90% of the GSC numbers of newly eclosed males, the mutant males maintain only 75% of the average GSC number of newly eclosed males.

InR^E19/InR⁰⁵⁵⁴⁵ males that are within 24 hours of eclosion have a reduced number of stage S2b spermatocyte cysts in the testis compared to wild-type males.

The average diameter of stage S6 InR^E19/InR⁰⁵⁵⁴⁵ spermatocytes is smaller than that of control spermatocytes at this stage.

(Ueishi et al., 2009)

The severity of cocaine-induced follicle degeneration in InR⁰⁵⁵⁴⁵/+ females is indistinguishable from that observed in wild-type females.

(Willard et al., 2006)

Homozygous clones in the eye contain ommatidia which are smaller than normal.

(Potter et al., 2001)

Heterozygous males and females show a moderate reduction in life expectancy compared to controls (from 32.3 to 23.7 and from 35.5 to 30.5 days respectively) and are normal in size. InR^E19/InR⁰⁵⁵⁴⁵ adults are dwarf in size. Developmental rate is slower than normal. Females show an 85% increase in life expectancy compared to controls (from 32.3 to 60.1 days). Males show a high mortality as early adults, but have reduced mortality at late ages, resulting in a life expectancy at 10 days that is 43% greater than that of controls. InR⁰⁵⁵⁴⁵/InR^E19 females require continuous exposure to methoprene to induce any vitellogenesis. Treatment with methoprene reduces the longevity of InR⁰⁵⁵⁴⁵/InR^E19 females towards wild-type levels.

(Tatar et al., 2001)

Embryonic CNS defects.

(Fernandez et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

InR⁰⁵⁵⁴⁵/InR³⁵³ has lethal phenotype, suppressible by Tsc1²⁹

(Gao and Pan, 2001)

NOT Enhancer of

Statement

Reference

InR⁰⁵⁵⁴⁵ is a non-enhancer of abnormal size | adult stage | somatic clone phenotype of Tsc1^Q600X

(Potter et al., 2001)

Suppressor of

Statement

Reference

InR⁰⁵⁵⁴⁵ is a suppressor of increased cell growth rate | somatic clone phenotype of Pten¹¹⁷

(Nowak et al., 2013)

InR⁰⁵⁵⁴⁵ is a suppressor of lethal phenotype of Ras85D^V12.sev, sev^S11.Tag:MYC

(Maixner et al., 1998)

NOT Suppressor of

Statement

Reference

InR⁰⁵⁵⁴⁵ is a non-suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

InR⁰⁵⁵⁴⁵ is a non-suppressor of visible | somatic clone phenotype of Tsc1^Q600X

(Potter et al., 2001)

InR³⁵/InR⁰⁵⁵⁴⁵ is a non-suppressor of increased cell size | somatic clone phenotype of Pten^dj189

(Gao et al., 2000)

Other

Statement

Reference

InR⁰⁵⁵⁴⁵/InR³⁵³, Tsc1²⁹ has partially lethal - majority live phenotype

(Gao and Pan, 2001)

InR⁰⁵⁵⁴⁵, Ras85D^V12.sev, sev^S11.Tag:MYC has viable phenotype

(Maixner et al., 1998)

Phenotype Manifest In

Enhancer of

Statement

Reference

InR⁰⁵⁵⁴⁵ is an enhancer of eye phenotype of chico^flp147E

(Bohni et al., 1999)

InR⁰⁵⁵⁴⁵ is an enhancer of wing phenotype of chico^flp147E

(Bohni et al., 1999)

InR⁰⁵⁵⁴⁵ is an enhancer of adult phenotype of chico^flp147E

(Bohni et al., 1999)

NOT Enhancer of

Statement

Reference

InR[+]/InR⁰⁵⁵⁴⁵ is a non-enhancer of photoreceptor neuron phenotype of S^IIN

(Hahn et al., 2013)

Suppressor of

Statement

Reference

InR⁰⁵⁵⁴⁵ is a suppressor of eye | somatic clone phenotype of Pten¹¹⁷

(Nowak et al., 2013)

NOT Suppressor of

Statement

Reference

InR[+]/InR⁰⁵⁵⁴⁵ is a non-suppressor of photoreceptor neuron phenotype of S^IIN

(Hahn et al., 2013)

InR⁰⁵⁵⁴⁵ is a non-suppressor of ommatidium | somatic clone phenotype of Tsc1^Q600X

(Potter et al., 2001)

InR³⁵/InR⁰⁵⁵⁴⁵ is a non-suppressor of wing margin bristle | somatic clone phenotype of Pten^dj189

(Gao et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

A InR⁰⁵⁵⁴⁵ mutant background only slightly reduces the overgrowth found in Pten¹¹⁷ mutant eye clones.

(Nowak et al., 2013)

EcR^M554fs/+; InR⁰⁵⁵⁴⁵/+ females do not show the cocaine-induced degeneration of vitellogenic follicles seen in either single heterozygote or wild-type flies.

(Willard et al., 2006)

Tsc1²⁹ suppresses the lethality of InR³⁵³/InR⁰⁵⁵⁴⁵ animals, allowing 39% animals to survive to adults.

(Gao and Pan, 2001)

Clones in the eye doubly mutant for InR⁰⁵⁵⁴⁵ and Tsc1^Q600X show the Tsc1^Q600X single mutant phenotype of enlarged ommatidia.

(Potter et al., 2001)

The phenotype of homozygous chico^flp147E flies is enhanced by a copy of InR⁰⁵⁵⁴⁵. Overall wing size is reduced by 17.7%, the number of cells reduced by 19.8%. In the eye, the number of ommatidia and size of the eye is also reduced.

(Bohni et al., 1999)

The lethality caused by sev^{S11.T:Hsap\MYC} in combination with Ras85D^V12.sev is suppressed by InR⁰⁵⁵⁴⁵.

(Maixner et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11661

ry⁵⁰⁶ P{PZ}InR⁰⁵⁵⁴⁵/TM3, ry^RK Sb¹ Ser¹

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: mod(mdg4)⁰³⁸⁵². Complements: E2f⁰⁷¹⁷². Complements: mod(mdg4)^j2B7. Complements: E2f^rM729.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

5545

(Chen et al., 1996)

InR(p5545)

(Tu et al., 2002)

InR-P5545

(McClure et al., 2011)

InR⁰⁵⁵⁴⁵

(Banzai and Nishimura, 2023, Nakamura et al., 2020, Neuman and Bashirullah, 2018, Park et al., 2014, Walker et al., 2013, Bolukbasi et al., 2012, Murillo-Maldonado et al., 2011, Biteau et al., 2010, Ueishi et al., 2009, Christensen et al., 2008.12.28, Christensen et al., 2008.12.28)

InR⁵⁵⁴⁵

(Monyak et al., 2017, Nowak et al., 2013, McClure et al., 2011)

InR^PZ

(Fernandez et al., 1995)

InR^p5545

(Dong et al., 2019, Kamakura, 2011, Aigaki et al., 2002, Wolkow, 2002, Tatar et al., 2001)

Inr[PZ]

(Clancy et al., 2001)

Insulin-like receptor⁰⁵⁵⁴⁵

(Willard et al., 2006)

dInR^PZ

(Rideout et al., 2012)

dinr^P5545

(Potter et al., 2001)

inr^l(3)05545

(Gao et al., 2000)

l(3)05545

(Spradling et al., 1999, Maixner et al., 1998)

l(3)05545⁰⁵⁵⁴⁵

lnR⁰⁵⁵⁴⁵

(Okamoto et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (35)

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