Open Close
General Information
Symbol
Dmel\Klp61F07012
Species
D. melanogaster
Name
FlyBase ID
FBal0009588
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
klp61F3, l(3)07012
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion of a P{PZ} element in the 5' untranslated region, 236bp upstream of the ATG start codon.

Insertion components
P{PZ}Klp61F07012
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

aster & neuroblast & larva

aster & spermatocyte

aster & spermatocyte (with Df(3L)bab-PG)

aster & spermatocyte (with Klp61F06345)

centriole & spermatocyte

centrosome & neuroblast & larva

centrosome & spermatocyte

centrosome & spermatocyte (with Df(3L)bab-PG)

centrosome & spermatocyte (with Klp61F06345)

meiotic telophase II & spermatocyte

mitotic cell cycle & spermatocyte

nuclear lamina & neuroblast & larva

nuclear lamina & spermatocyte

spindle & neuroblast & larva

spindle & spermatocyte

spindle & spermatocyte (with Df(3L)bab-PG)

spindle & spermatocyte (with Klp61F06345)

spindle & spermatocyte (with Klp61Furc-1)

Detailed Description
Statement
Reference

Homozygous larval brain neuroblasts show a high degree of aneuploidy.

Embryos derived from heterozygous females show abnormalities in approximately 20% of mitotic spindles during syncytial divisions. The majority of the defects occur in cycles 1-9. The most frequent defects are anastral (4.89%) and monopolar spindles (6.77%).

Embryos derived from Klp61F07012/+ females carrying Klp61F3YF.Ubi-p63E.T:Hsap\MYC show a higher incidence of spindles with multiple microtubule organising centers during cycles 1-9 than seen in control embryos. The mutant embryos also show "promiscuous microtubules", including microtubule spurs and microtubules interacting between neighbouring spindles, during cycles 11-13. These promiscuous microtubules are seen in metaphase (at a similar level as occurs in embryos derived from Klp61F07012/+ females with no transgene) and also in anaphase (at a significantly increased level than occurs in embryos derived from Klp61F07012/+ females with no transgene).

Klp61F07012 homozygotes and Klp61F07012/Klp61F06836 trans-heterozygotes display larval lethality.

Klp61FUbi-p63E.T:Avic\GFP rescues the lethality of homozygous Klp61F07012 and trans-heterozygous Klp61F07012/Klp61F06836 mutants. The rescued flies are fertile and can be propagated as stable transgenic lines. The rescued embryos display a decrease in the rate of prometaphase-to-metaphase spindle elongation compared with wild type embryos, which leads to a small decrease in subsequent spindle length.

15% of Klp61F07012 mutant spindles are biastral in larval stage brains. The nuclear lamina in monopolar Klp61F07012 cells in larval stage brains show involutions that extend towards centrally located centrosomes. This suggests that the nuclear lamina is directly or indirectly attached to centrosomal material and/or centrosomal asters. Acentrosomal meiotic spermatocytes of Klp61F07012 homozygotes, Klp61F07012/Klp61F06345 transheterozygotes, and Klp61F07012/Df(3L)bab-PG exhibit microtubules, but do not show microtubule asters or detectable spindle structures. Spermatocytes containing centrosomes display a range of spindle defects, classic monopolar spindles are found in less than 10% of spindle structures in Klp61F07012 mutants, substantially lower than the 50-75% frequency found in somatic cells of Klp61F07012 mutants. Most meiotic spindles had characteristics of both monopolar and monastral bipolar spindles, showing microtubules splayed into broad spindle poles that lack detectable γ-tubulin or showing splintered spindle poles with foci of γ-tubulin at the ends of microtubule bundles. Despite the abnormal organisation of microtubules, spermatocytes in telophase show a cytokinetic furrow that divides the spermatocyte unequally, with one daughter receiving most or all of the bivalents and centrosomes. Telophase is also abnormal in that bivalents typically remain paired. Fusiform structures, indicative of a bipolar spindle in wild-type spermatocytes are not detected in Klp61F07012 and Klp61F07012/Klp61Furc-1 transheterozygote spermatocytes. In Klp61F07012 mutant spermatocytes the nuclear lamina shows deep involutions that are occupied by closely apposed centrosomes. The nuclear lamina appears to collapse around bivalents in spermatocytes and form micronuclei, irrespective of the presence or absence of centrosomes. Klp61F07012 meiotic spermatocytes show immunostaining of aggregates that are typically associated with chromatin within micronuclei.

The fusome in Klp61F06345 testes appears to be fragmented.

The brains of homozygous larvae have a reduced frequency of anaphase figures, and an increased frequency of polyploid figures and overcondensed chromosomes compared to wild-type. The brains and imaginal discs are small compared to wild-type in approximately 50% of the larvae. Nearly all homozygous spermatocytes are equal in size and contain equivalent amounts of chromatin. The spermatocytes contain a variable number of centriole pairs, the most common being 0 (35% of spermatocytes), 2 (25% of spermatocytes) and 4 (24% of spermatocytes) centriole pairs, and 10% of spermatocytes contain an odd number of centriole pairs.

Homozygotes and hemizygotes die as late third instar larvae or early pupae. Neuroblasts isolated from third instar homozygous larvae show an increased mitotic index and complete lack of anaphase figures, indicating metaphase arrest. Chromosomes are hypercondensed compared to wild-type, monopolar spindles are seen and highly polyploid cells are common.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Enhancer of
Statement
Reference

Klp61F[+]/Klp61F07012 is an enhancer of visible phenotype of Hsap\MAPTGMR.Ex.PJ

NOT Suppressor of
Statement
Reference

Klp61F[+]/Klp61F07012 is a non-suppressor of visible phenotype of Scer\GAL4GMR.PU, Zzzz\CAGQ108.UAS

Other
Statement
Reference
Phenotype Manifest In
Suppressed by
Statement
Reference

Klp61F07012 has spindle & neuroblast & larva phenotype, suppressible by ncd1

Enhancer of
Statement
Reference

Klp61F[+]/Klp61F07012 is an enhancer of eye phenotype of Hsap\MAPTGMR.Ex.PJ

NOT Suppressor of
Statement
Reference

Klp61F[+]/Klp61F07012 is a non-suppressor of eye phenotype of Scer\GAL4GMR.PU, Zzzz\CAGQ108.UAS

Other
Statement
Reference

Klp61Furc-1/Klp61F07012, ncd1 has nuclear lamina & neuroblast & larva phenotype

Klp61F07012/Df(3L)bab-PG, ncd1 has nuclear lamina & neuroblast & larva phenotype

Additional Comments
Genetic Interactions
Statement
Reference

One copy of Klp61F07012 does not suppress the apoptosis seen in flies overexpressing hid.

Klp61F07012/Df(3L)bab-PG ncd1 cells have a monopolar organisation of chromosomes and centrosomes, showing nuclear lamina involutions that extend towards centrally located centrosomes. Klp61F07012 ncd1/Klp61Furc-1 ncd1 cells have a monopolar organisation of chromosomes and centrosomes, showing nuclear lamina involutions that extend towards centrally located centrosomes. The nuclear lamina in interphase cells of Klp61Furc-1 ncd1/Klp61F07012 ncd1 double mutant larval stage brains is indistinguishable from that of wild type. However, in most mitotic double mutant cells, a nuclear lamina can not be detected or is very disorganised (in contrast to wild type). More than 96% of Klp61F07012 ncd1 double mutant spindles are biastral.

Xenogenetic Interactions
Statement
Reference

One copy of Klp61F07012 enhances the rough eye phenotype seen in flies expressing Hsap\MAPTGMR.Ex.PJ.

Klp61F07012 does not induce a rough eye phenotype in flies expressing Hsap\MAPTScer\UAS.S2A under the control of Scer\GAL4GMR.PS.

One copy of Klp61F07012 does not suppress the rough eye phenotype seen in flies expressing Zzzz\CAGQ108.Scer\UAS under the control of Scer\GAL4GMR.PU.

Complementation and Rescue Data
Comments

Klp61F3YF.Ubi-p63E.T:Hsap\MYC does not rescue the pupal lethality of Klp61F07012 homozygotes or the incidence of aneuploidy in Klp61F07012 larval neuroblasts.

Klp61FUbi-p63E.T:Hsap\MYC partially rescues the pupal lethality of Klp61F07012 homozygotes and rescues the aneuploidy seen in Klp61F07012 larval neuroblasts.

Klp61FUbi-p63E.T:Hsap\MYC rescues both anastral and monopolar spindles in embryos derived from heterozygous Klp61F07012 females.

Klp61F3YF.Ubi-p63E.T:Hsap\MYC rescues anastral spindles in embryos derived from heterozygous Klp61F07012 females. However, the monopolar spindle defects are not rescued in these animals.

Klp61FUbi-p63E.T:Avic\GFP rescues the lethality of homozygous Klp61F07012 and trans-heterozygous Klp61F07012/Klp61F06836 mutants. The rescued flies are fertile and can be propagated as stable transgenic lines.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

A. Spradling.

Comments
Comments

Excision of the P{PZ} element is accompanied by reversion of lethality and the abnormal mitotic phenotype.

Complements: l(3)0210402104. Complements: l(3)0264002640. Complements: l(3)0264003544. Complements: l(3)0264004563. Complements: l(3)0264004808. Complements: l(3)0646506465. Complements: l(3)L2049L2049. Complements: l(3)L2290L2290. Complements: l(3)L2049L3879a. Complements: mtacp1j4A6. Complements: l(3)02640j4E3.

Klp61F alleles form the following phenotypic series, from the most to the least severe: Klp61Furc-1 > Klp61Furc-3 > Klp61F06836, Klp61F07012 > Klp61F06345 > Klp61Furc-4.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
References (19)