FlyBase Allele Report: Dmel\E2f1[07172]

(Li et al., 2021, Baumgardt et al., 2014, Gehrke et al., 2010, Sinenko et al., 2010, Spradling et al., 1999, Brook et al., 1996, Duronio and O'Farrell, 1995, Duronio et al., 1995, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Lrrk^I1915T.UAS

(Gehrke et al., 2010)

ameliorates acute myeloid leukemia

modeled by Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa

(Sinenko et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsal appendage (with E2f1ⁱ²)

(Geng and MacDonald, 2007)

egg (with E2f1ⁱ²)

(Geng and MacDonald, 2007)

embryonic thorax & neuron

(Berger et al., 2005)

eye

(Brook et al., 1996)

eye-antennal disc

(Brook et al., 1996)

eye photoreceptor cell

(Brook et al., 1996)

neuroblast NB6-4

(Berger et al., 2005)

nurse cell (with E2f1ⁱ¹)

(Royzman et al., 2002)

ommatidium

(Brook et al., 1996)

oocyte (with E2f1ⁱ²)

(Geng and MacDonald, 2007)

rhabdomere

(Brook et al., 1996)

wing disc | somatic clone | third instar larval stage

(Zhang et al., 2021)

Detailed Description

Statement

Reference

Third instar larval wing disc clones homozygous for E2f1⁰⁷¹⁷²have fewer cells than controls.

(Zhang et al., 2021)

One copy of E2f⁰⁷¹⁷² has no effect on hemocyte proliferation in third instar larvae.

(Sinenko et al., 2010)

E2f1⁰⁷¹⁷² germline stem cell (GSC) clones behave similarly to control GSC clones and do not dramatically affect GSC maintenance.

(Jin et al., 2008)

E2fⁱ²/E2f⁰⁷¹⁷² trans-heterozygotes are viable and can complete oogenesis. Eggs laid by these females display a variety of dorsoventral polarity defects, including eggshells with fused dorsal appendages (ventralised, 22%), eggshells with fused but slightly expanded dorsal appendages (between dorsalised and ventralised, 39%), and eggshells with fused but widely spread dorsal appendages (strongly dorsalised, 11%). In addition, 8% of the eggs laid by E2fⁱ²/E2f⁰⁷¹⁷² females have greatly degenerated dorsal appendages, which obscure the dorsoventral polarity pattern of these eggshells.

(Geng and MacDonald, 2007)

In E2f⁰⁷¹⁷² mutants, unlike CycE mutants, no homeotic transformation of NB6-4t to NB6-4a is observed. While the number of NB6-4 derived glia in the thorax is unaffected, the number of NB6-4 derived neurons per thoracic segment is reduced from 5-6 to 2-4 in approximately 85% of embryos.

(Berger et al., 2005)

In E2fⁱ¹/E2f⁰⁷¹⁷² egg chambers, unlike wild-type (where nurse cells undergo apoptosis during stages 12 to 13 and have degenerated by stage 14), a cluster of nurse cells remain attached to the stage 14 oocyte and having failed to under go apoptosis. Some mutant eggs chambers, one or a few persisting nurse cells do show signs of apoptosis, but not until stage 14.

(Royzman et al., 2002)

E2f1/Dp-dependent G[[1]]-S transcription is disrupted late in embryogenesis, but S phase takes place. Lethality occurs at late larval, early pupal stages. Lethality of E2f1⁹¹/E2f1⁰⁷¹⁷² occurs at late larval, early pupal stages. Larval growth is dramatically delayed taking 288 to 432 hours from larvae to pupation compared to 120 hours for wild type. Five days after egg laying larvae are very sluggish and much smaller in size than wild type. Salivary glands, imaginal discs and the brain are greatly reduced in size in 5 day old larvae but they do grow to wild type proportions by the end of their larval life. Larvae also exhibit melanotic pseudotumours, they first appear 7 days after egg laying and they grow and darken as the larvae age.

(Royzman et al., 1997)

Clones induced in the eye in the first or second larval instar are not detected in the adult eye. They can be detected in the eye disc, but are much smaller than the adjacent twin spots, with fewer cells. These cells have initiated neuronal differentiation. Resulting ommatidia have incomplete sets of photoreceptors, and photoreceptors that are present often have abnormal morphology. Rhabdomeres are small in diameter, the rhabdomeres of the outer photoreceptors may project centrally and the photoreceptors do not extend the full length of the retina.

(Brook et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

E2f1⁹¹/E2f1⁰⁷¹⁷² has lethal phenotype, enhanceable | maternal effect by CycE[+]/CycE⁰⁵²⁰⁶

(Royzman et al., 1997)

NOT suppressed by

Statement

Reference

E2f1⁰⁷¹⁷² has decreased cell number | somatic clone | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Tub.PU/cbt^UAS.cMa

(Zhang et al., 2021)

NOT Enhancer of

Statement

Reference

E2f1⁰⁷¹⁷² is a non-enhancer of visible phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

E2f1⁰⁷¹⁷² is a non-enhancer of visible phenotype of Dp^GMR.PD, E2f1^GMR.PD

(Staehling-Hampton et al., 1999)

Suppressor of

Statement

Reference

E2f[+]/E2f1⁰⁷¹⁷² is a suppressor of abnormal neuroanatomy phenotype of Lrrk^I1915T.UAS, Scer\GAL4^ple.PF

(Gehrke et al., 2010)

E2f[+]/E2f1⁰⁷¹⁷² is a suppressor of abnormal neuroanatomy | recessive phenotype of mir-let7^Δ.cSa

(Gehrke et al., 2010)

E2f[+]/E2f1⁰⁷¹⁷² is a suppressor | partially of increased cell number | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

E2f[+]/E2f1⁰⁷¹⁷² is a suppressor of increased cell death phenotype of Dref^UAS.cSa, Scer\GAL4^GMR.PS

(Hirose et al., 2001)

NOT Suppressor of

Statement

Reference

E2f1⁰⁷¹⁷² is a non-suppressor of visible phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

E2f1⁰⁷¹⁷² is a non-suppressor of visible phenotype of Mmus\Mdm2^UAS.cFa, Scer\GAL4^GMR.PF

(Folberg-Blum et al., 2002)

E2f1⁰⁷¹⁷² is a non-suppressor of abnormal size | adult stage phenotype of Mmus\Mdm2^UAS.cFa, Scer\GAL4^GMR.PF

(Folberg-Blum et al., 2002)

E2f1⁰⁷¹⁷² is a non-suppressor of visible phenotype of Dp^GMR.PD, E2f1^GMR.PD

(Staehling-Hampton et al., 1999)

Other

Statement

Reference

Dp^UAS.cDa, E2f1⁰⁷¹⁷², E2f1^UAS.cNa, Scer\GAL4^Tub.PU has increased cell number | somatic clone | third instar larval stage phenotype

(Zhang et al., 2021)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

E2f1⁰⁷¹⁷² has wing disc | somatic clone | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Tub.PU/cbt^UAS.cMa

(Zhang et al., 2021)

Enhancer of

Statement

Reference

E2f[+]/E2f1⁰⁷¹⁷² is an enhancer of egg phenotype of Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C}

(Geng and MacDonald, 2007)

E2f[+]/E2f1⁰⁷¹⁷² is an enhancer of egg chorion phenotype of Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C}

(Geng and MacDonald, 2007)

E2f1⁰⁷¹⁷², Scer\GAL4^GMR.PF is an enhancer of eye phenotype of E2f2^UAS.cFa, Scer\GAL4^GMR.PF

(Frolov et al., 2001)

NOT Enhancer of

Statement

Reference

E2f1⁰⁷¹⁷² is a non-enhancer of eye phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

E2f1⁰⁷¹⁷² is a non-enhancer of phenotype of Myb^unspecified

(Fung et al., 2003)

Suppressor of

Statement

Reference

E2f[+]/E2f1⁰⁷¹⁷² is a suppressor of dopaminergic neuron phenotype of mir-let7^Δ.cSa

(Gehrke et al., 2010)

E2f[+]/E2f1⁰⁷¹⁷² is a suppressor of dopaminergic neuron phenotype of Lrrk^I1915T.UAS, Scer\GAL4^ple.PF

(Gehrke et al., 2010)

E2f[+]/E2f1⁰⁷¹⁷² is a suppressor | partially of embryonic/larval hemocyte | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

E2f1⁰⁷¹⁷² is a suppressor of female germline stem cell | germline clone phenotype of bam^Δ86

(Jin et al., 2008)

E2f1⁰⁷¹⁷² is a suppressor of wing phenotype of Mmus\Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096, slmb^e4-1

(Heriche et al., 2003)

E2f1⁰⁷¹⁷² is a suppressor of wing phenotype of Mmus\Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096, slmb^P245

(Heriche et al., 2003)

E2f[+]/E2f1⁰⁷¹⁷² is a suppressor of eye phenotype of Dref^UAS.cSa, Scer\GAL4^GMR.PS

(Hirose et al., 2001)

NOT Suppressor of

Statement

Reference

E2f1⁰⁷¹⁷² is a non-suppressor of eye phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

E2f1⁰⁷¹⁷² is a non-suppressor of phenotype of Myb^unspecified

(Fung et al., 2003)

E2f1⁰⁷¹⁷² is a non-suppressor of eye phenotype of Mmus\Mdm2^UAS.cFa, Scer\GAL4^GMR.PF

(Folberg-Blum et al., 2002)

Other

Statement

Reference

Dp^UAS.cDa, E2f1⁰⁷¹⁷², E2f1^UAS.cNa, Scer\GAL4^Tub.PU has wing disc | somatic clone | third instar larval stage phenotype

(Zhang et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Third instar larval wing disc clones homozygous for E2f1⁰⁷¹⁷² and that also co-express E2f1^UAS.cNa and Dp^UAS.cDa under the control of Scer\GAL4^Tub.PU have more cells than controls.

(Zhang et al., 2021)

Heterozygosity for E2f⁰⁷¹⁷² suppresses the dopaminergic neuronal phenotypes in animals expressing Lrrk^{I1915T.Scer\UAS} under the control of Scer\GAL4^ple.PF.

Heterozygosity for E2f⁰⁷¹⁷² suppresses the dopaminergic neuronal phenotypes of homozygous let-7^Δ.cSa animals.

(Gehrke et al., 2010)

bam^Δ86 E2f1⁰⁷¹⁷² double mutant germline stem cell (GSC) clones are much less competitive than bam^Δ86 single mutant GSCs.

(Jin et al., 2008)

A heterozygous E2f⁰⁷¹⁷² background strongly enhances the dorsalisation phenotype seen when Imp^Scer\UAS.cGa is expressed under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (on the second).

(Geng and MacDonald, 2007)

The addition of E2f⁰⁷¹⁷² has no effect on Myb^unspecified viability.

(Fung et al., 2003)

The rough eye phenotype seen upon expression of Dref^Scer\UAS.cSa in the developing eye under the control of Scer\GAL4^GMR.PS is suppressed in an E2f⁰⁷¹⁷² heterozygous background.

(Hirose et al., 2001)

Lethality of E2f1⁹¹/E2f1⁰⁷¹⁷² transheterozygotes is dramatically enhanced by reducing the maternal dosage of CycE (CycE⁰⁵²⁰⁶).

(Royzman et al., 1997)

Xenogenetic Interactions

Statement

Reference

One copy of E2f⁰⁷¹⁷² moderately suppresses the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cSa is expressed under the control of Scer\GAL4^Hml.Δ.

(Sinenko et al., 2010)

The presence of E2f1⁰⁷¹⁷² as well as slmb^e4-1 reverses the enhancement of the Mmus\Cul1^Scer\UAS.cHa/Scer\GAL4^Bx-MS1096 wing size phenotype by a slmb^e4-1 background.

(Heriche et al., 2003)

The eye phenotype caused by expression of Mmus\Mdm2^Scer\UAS.cFa under the control of Scer\GAL4^GMR.PF is not rescued if the flies are also carrying E2f⁰⁷¹⁷².

(Folberg-Blum et al., 2002)

Complementation and Rescue Data

Fails to complement

E2f1^{E(Sev-CycE)3A}

(Lane et al., 2000)

E2f1^j3C2

(BDGP Project Members, 1994-1999)

E2f1^j3B1

(BDGP Project Members, 1994-1999)

E2f1^rM729

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

108875

ry⁵⁰⁶ P{PZ}E2f1⁰⁷¹⁷²/TM3, ry^RK Sb¹ Ser¹

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: mod(mdg4)⁰³⁸⁵². Complements: Hsrω⁰⁵²⁴¹. Complements: InR⁰⁵⁵⁴⁵. Complements: mod(mdg4)⁰⁷⁰³⁸.

(BDGP Project Members, 1994-1999)

Transposase-mediated excision of the P{PZ} produces ry^- flies that lack both the insertion and the lethal phenotype.

(Duronio et al., 1995)

mir-11^Δ1 complements E2f⁰⁷¹⁷².

(Truscott et al., 2011)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (21)

Reported As

Symbol Synonym

07172

(Geng and MacDonald, 2007, Mukherjee et al., 2006)

E2F1⁰⁷¹⁷²

(Khurana et al., 2006)

E2F⁰⁷¹⁷²

(Folberg-Blum et al., 2002)

E2F⁷¹⁷²

(Heriche et al., 2003, Asano and Wharton, 1999, Staehling-Hampton et al., 1999)

E2F⁷²⁷¹

(Asano et al., 1996)

E2f1⁰⁷¹⁷²

(Macabenta et al., 2022, Wang and Baker, 2015)

E2f1⁷¹⁷²

(Sloan et al., 2012, Shibutani et al., 2007)

E2f<up>07 172</up>

(Sinenko et al., 2010)

E2f⁰⁷¹⁷²

(Yaghmaeian Salmani et al., 2018, Bivik et al., 2015, Baumgardt et al., 2014, Sinenko et al., 2010)

E2f⁷¹⁰²

(Hirose et al., 2001)

E2f⁷¹⁷²

(Brook et al., 1996)

dE2F1⁰⁷¹⁷²

(Jin et al., 2008)

dE2F1⁷¹⁷²

(Thacker et al., 2003, Royzman et al., 2002, Lane et al., 2000)

dE2F⁷¹⁷²

(Berger et al., 2005, Kwon et al., 2001, Royzman et al., 1999, Tao et al., 1999, Royzman et al., 1997, Duronio et al., 1995)

dE2f1⁰⁷¹⁷²

(Truscott et al., 2011)

dE2f1⁷¹⁷²

(Truscott et al., 2014)

e2f1⁰⁷¹⁷²

(Li et al., 2024, Li et al., 2021, Wang et al., 2020)

e2f1⁷¹⁷²

(Zhang et al., 2021)

e2f⁷¹⁷²

(Fung et al., 2003)

l(3)07172

(Spradling et al., 1999)

l(3)07172⁰⁷¹⁷²

Name Synonyms

Secondary FlyBase IDs

References (42)

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