Allele Dmel\vn¹⁰⁵⁶⁷

General Information
Symbol	Dmel\vn¹⁰⁵⁶⁷	Species	D. melanogaster
Name		FlyBase ID	FBal0009626
Feature type	allele	Associated gene	Dmel\vn
Also Known As	vn^P1749

Map ( GBrowse )	Untitled Document
Allele class	loss of function allele
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	loss of function allele (Yarnitzky et al., 1997)
Mutagen	P-element activity
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3L:5,837,369..5,837,369 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Urban et al., 2004, Yarnitzky et al., 1998, Yarnitzky et al., 1997, Eggert et al., 1998, Jiang and Edgar, 2009)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{PZ} insertion within the first non-coding exon. (Yarnitzky et al., 1997)
Caused by insertion	P{PZ}vn¹⁰⁵⁶⁷ (BDGP Project Members, 1994-1999, Spradling et al., 1999, Urban et al., 2004, Yarnitzky et al., 1998, Yarnitzky et al., 1997, Eggert et al., 1998, Jiang and Edgar, 2009)
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell number \| larval stage (with vn^γ7) (Jiang and Edgar, 2009) decreased cell number \| late third instar larval stage \| recessive (Jiang and Edgar, 2009) lethal \| pharate adult stage (with vn^γ7) (Jiang and Edgar, 2009) lethal \| recessive (Spradling et al., 1999)
Phenotype Manifest In
	abdominal 2 dorsal acute muscle 1 \| precursor (Yarnitzky et al., 1998) abdominal 3 dorsal acute muscle 1 \| precursor (Yarnitzky et al., 1998) abdominal 4 dorsal acute muscle 1 \| precursor (Yarnitzky et al., 1998) abdominal 5 dorsal acute muscle 1 \| precursor (Yarnitzky et al., 1998) abdominal 6 dorsal acute muscle 1 \| precursor (Yarnitzky et al., 1998) abdominal 7 dorsal acute muscle 1 \| precursor (Yarnitzky et al., 1998) adult midgut precursor cell \| larval stage (with vn^γ7) (Jiang and Edgar, 2009) adult midgut precursor cell \| late third instar larval stage (Jiang and Edgar, 2009) dorsal oblique muscle (Yarnitzky et al., 1997) embryonic/larval midgut \| embryonic stage (Szuts et al., 1998) embryonic/larval somatic muscle (Yarnitzky et al., 1998) gastric caecum (Szuts et al., 1998) lch5 ligament attachment cell (with Df(3L)v65c) (Inbal et al., 2004) midgut constriction 1 (Szuts et al., 1998) midgut constriction 2 (Szuts et al., 1998) midgut constriction 3 (Szuts et al., 1998) ventral longitudinal muscle (Yarnitzky et al., 1997) ventral oblique muscle (Yarnitzky et al., 1997)
Detailed Description
	Statement Reference Whereas wild-type adult midgut progenitor cells (AMPs) form many large clusters, few AMP clusters are found in late larval vn[10567] and vn[10567]/vn[γ7] mutant midguts. Most vn[10567]/vn[γ7] mutants die as pharate adults. Lineage analysis of marked clones reveals that vn[10567]/vn[γ7] mutants display a failure of proliferation of AMPs during early larval stages, although the number of AMPs generated initially during embryogenesis is not different between mutants and wild-type controls. The size of the few remaining AMP clusters in vn[10567]/vn[γ7] mutants is relatively normal, suggesting that the late phase of AMP proliferation is largely unaffected in these mutants. Expression of vn[Scer\UAS.cSa] in larval enterocytes driven by Scer\GAL4[Myo31DF-NP0001] not only rescues the adult midgut progenitor cell phenotype of vn[10567] mutants, but it also causes ectopic AMP cell proliferation. (Jiang and Edgar, 2009) Late stage vn¹⁰⁵⁶⁷/Df(3L)v65c embryos lack lch5 ligament attachment cells. (Inbal et al., 2004) Border cells still migrate dorsally when all dorsal follicle cells are mutant for vn¹⁰⁵⁶⁷ in females containing homozygous clones. (Duchek and Rorth, 2001) Shows very mild midgut phenotype. vn¹⁰⁵⁶⁷/Df(3L)γ3 causes lack of midgut constrictions and failure of elongation of gastric caeca. (Szuts et al., 1998) A significant number of somatic muscle fibres show multiple, mis-guided membrane extensions at their leading edges. The DA1 muscle precursor is sometimes missing. (Yarnitzky et al., 1998) Abnormal muscle phenotype. Myotubes of mutant embryos are elongated and continuously send filopodia in random directions. (Yarnitzky et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference vn[+]/vn¹⁰⁵⁶⁷ is an enhancer of visible phenotype of Scer\GAL4^ey.PH, brm^{K804R.Scer\UAS.T:Ivir\HA1} (Armstrong et al., 2005)
Suppressor of
Statement Reference vn[+]/vn¹⁰⁵⁶⁷ is a suppressor of visible phenotype of Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} (Oishi et al., 2006)
Other
Statement Reference brm[+]/brm², vn¹⁰⁵⁶⁷ has visible phenotype (Armstrong et al., 2005) brm², vn[+]/vn¹⁰⁵⁶⁷ has visible \| dominant phenotype (Armstrong et al., 2005)
Phenotype Manifest In
Enhanced by
Statement Reference vn¹⁰⁵⁶⁷ has abdominal 2 dorsal acute muscle 1 \| precursor phenotype, enhanceable by Scer\GAL4^how-24B/Scer\GAL4^how-24B/Egfr^DN.Scer\UAS (Yarnitzky et al., 1998) vn¹⁰⁵⁶⁷ has abdominal 2 dorsal acute muscle 1 \| precursor phenotype, enhanceable by spi³ (Yarnitzky et al., 1998) vn¹⁰⁵⁶⁷ has abdominal 3 dorsal acute muscle 1 \| precursor phenotype, enhanceable by Scer\GAL4^how-24B/Scer\GAL4^how-24B/Egfr^DN.Scer\UAS (Yarnitzky et al., 1998) vn¹⁰⁵⁶⁷ has abdominal 3 dorsal acute muscle 1 \| precursor phenotype, enhanceable by spi³ (Yarnitzky et al., 1998) vn¹⁰⁵⁶⁷ has abdominal 4 dorsal acute muscle 1 \| precursor phenotype, enhanceable by Scer\GAL4^how-24B/Scer\GAL4^how-24B/Egfr^DN.Scer\UAS (Yarnitzky et al., 1998) vn¹⁰⁵⁶⁷ has abdominal 4 dorsal acute muscle 1 \| precursor phenotype, enhanceable by spi³ (Yarnitzky et al., 1998) vn¹⁰⁵⁶⁷ has abdominal 5 dorsal acute muscle 1 \| precursor phenotype, enhanceable by Scer\GAL4^how-24B/Scer\GAL4^how-24B/Egfr^DN.Scer\UAS (Yarnitzky et al., 1998) vn¹⁰⁵⁶⁷ has abdominal 5 dorsal acute muscle 1 \| precursor phenotype, enhanceable by spi³ (Yarnitzky et al., 1998) vn¹⁰⁵⁶⁷ has abdominal 6 dorsal acute muscle 1 \| precursor phenotype, enhanceable by Scer\GAL4^how-24B/Scer\GAL4^how-24B/Egfr^DN.Scer\UAS (Yarnitzky et al., 1998) vn¹⁰⁵⁶⁷ has abdominal 6 dorsal acute muscle 1 \| precursor phenotype, enhanceable by spi³ (Yarnitzky et al., 1998) vn¹⁰⁵⁶⁷ has abdominal 7 dorsal acute muscle 1 \| precursor phenotype, enhanceable by Scer\GAL4^how-24B/Scer\GAL4^how-24B/Egfr^DN.Scer\UAS (Yarnitzky et al., 1998) vn¹⁰⁵⁶⁷ has abdominal 7 dorsal acute muscle 1 \| precursor phenotype, enhanceable by spi³ (Yarnitzky et al., 1998)
Enhancer of
Statement Reference vn[+]/vn¹⁰⁵⁶⁷ is an enhancer of eye phenotype of Scer\GAL4^ey.PH, brm^{K804R.Scer\UAS.T:Ivir\HA1} (Armstrong et al., 2005) vn[+]/vn¹⁰⁵⁶⁷ is an enhancer of ommatidium phenotype of Scer\GAL4^ey.PH, brm^{K804R.Scer\UAS.T:Ivir\HA1} (Armstrong et al., 2005) vn¹⁰⁵⁶⁷/vn¹⁰⁵⁶⁷ is an enhancer of denticle belt phenotype of rho^unspecified (Urban et al., 2004, Urban et al., 2004) vn¹⁰⁵⁶⁷/vn¹⁰⁵⁶⁷ is an enhancer of embryonic/first instar larval cuticle phenotype of rho^unspecified (Urban et al., 2004, Urban et al., 2004) vn¹⁰⁵⁶⁷ is an enhancer of dorsal appendage phenotype of Ras85D^ix12a (Wasserman and Freeman, 1998) vn¹⁰⁵⁶⁷ is an enhancer of egg phenotype of Ras85D^ix12a (Wasserman and Freeman, 1998)
Suppressor of
Statement Reference vn[+]/vn¹⁰⁵⁶⁷ is a suppressor of wing vein \| ectopic phenotype of Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} (Oishi et al., 2006)
Other
Statement Reference brm², vn[+]/vn¹⁰⁵⁶⁷ has humeral bristle phenotype (Armstrong et al., 2005) brm², vn¹⁰⁵⁶⁷ has humeral bristle phenotype (Armstrong et al., 2005) Gug¹⁴⁹⁶⁷, rho^PΔ5, vn¹⁰⁵⁶⁷ has wing vein \| somatic clone phenotype (Charroux et al., 2006, Charroux et al., 2006, Charroux et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference Although extra wing vein material is induced in Gug¹⁴⁹⁶⁷ clones, wing vein differentiation is blocked in vn¹⁰⁵⁶⁷ rho^PΔ5 Gug¹⁴⁹⁶⁷ triple mutant clones. (Charroux et al., 2006) 2/3 of vn¹⁰⁵⁶⁷; rho^unspecified double homozygous embryo cuticles show a general loss of cuticle integrity. Of the remaining 1/3 of cuticles the percentage with >2 denticle belt fusions is increased from <10% for rho^unspecified to >30%. (Note, while the authors do not name an rho allele for this analysis, they do claim to have used a null allele.) (Urban et al., 2004) The loss of the DA1 muscle precursor in vn¹⁰⁵⁶⁷ embryos is enhanced if they are also carrying Egfr^DN.Scer\UAS expressed under the control of Scer\GAL4^how-24B. The loss of the DA1 muscle precursor seen in vn¹⁰⁵⁶⁷ embryos is dominantly enhanced by spi³. (Yarnitzky et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	vn^rF264 (BDGP Project Members, 1994-1999)
Rescued by	vn¹⁰⁵⁶⁷ is rescued by Scer\GAL4^esg-NP7397/vn^Scer\UAS.cSa (Jiang and Edgar, 2009) vn¹⁰⁵⁶⁷ is rescued by Scer\GAL4^how-24B/vn^Scer\UAS.cSa/Scer\GAL4^how-24B (Jiang and Edgar, 2009) vn¹⁰⁵⁶⁷ is rescued by Scer\GAL4^{Myo31DF-NP0001}/Scer\GAL4^{Myo31DF-NP0001}/vn^Scer\UAS.cSa (Jiang and Edgar, 2009)
Comments	Expression of vn[Scer\UAS.cSa] in larval enterocytes driven by Scer\GAL4[Myo31DF-NP0001] not only rescues the adult midgut progenitor cell phenotype of vn[10567] mutants, but it also causes ectopic AMP cell proliferation. Expression of vn[Scer\UAS.cSa] in adult midgut progenitor cells (AMPs) rescues the AMP cell proliferation phenotype of vn[10567] mutants. Expression of vn[Scer\UAS.cSa] in visceral muscle driven by Scer\GAL4[how-24B] rescues the adult midgut progenitor cell phenotype of vn[10567] mutants. (Jiang and Edgar, 2009)
Stocks ( 1 )
Bloomington	11749 cn¹; P{PZ}vn¹⁰⁵⁶⁷ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹
Notes on Origin
Discoverer	A. Spradling.

Comments
	Precise excision of the P{PZ} insertion completely reverses the muscle phenotype and results in full viability. Results indicate the insertion is responsible for the phenotype. (Yarnitzky et al., 1997) Complements: Bre1⁰¹⁶⁴⁰. Complements: l(3)02331⁰²³³¹. Complements: l(3)05143⁰⁵¹⁴³. Complements: l(3)neo11¹. (BDGP Project Members, 1994-1999)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	l(3)10567 (Spradling et al., 1999) l(3)10567¹⁰⁵⁶⁷ l(3)vn10567 (Jin et al., 2005) P1749 (Yarnitzky et al., 1997) vn¹⁰⁵⁶⁷ (Charroux et al., 2006, Oishi et al., 2006) vn^P1749 (Inbal et al., 2004, Yarnitzky et al., 1998, Szuts et al., 1998, Jiang and Edgar, 2009)
Name Synonym
Secondary FlyBase IDs

References ( 18 )
Research paper	Jiang and Edgar, 2009, Development 136(3): 483--493 EGFR signaling regulates the proliferation of Drosophila adult midgut progenitors. [FBrf0206542] Charroux et al., 2006, Dev. Biol. 291(2): 278--290 Atrophin contributes to the negative regulation of epidermal growth factor receptor signaling in Drosophila. [FBrf0190100] Oishi et al., 2006, Hum. Mol. Genet. 15(4): 543--553 Transgenic Drosophila models of Noonan syndrome causing PTPN11 gain-of-function mutations. [FBrf0190828] Armstrong et al., 2005, Genetics 170(4): 1761--1774 Genetic Screens for Enhancers of brahma Reveal Functional Interactions Between the BRM Chromatin-Remodeling Complex and the Delta-Notch Signal Transduction Pathway in Drosophila. [FBrf0187627] Jin et al., 2005, Development 132(18): 4075--4085 Drosophila Myt1 is a Cdk1 inhibitory kinase that regulates multiple aspects of cell cycle behavior during gametogenesis. [FBrf0187466] Inbal et al., 2004, Dev. Cell 7(2): 241--250 Recruitment of ectodermal attachment cells via an EGFR-dependent mechanism during the organogenesis of Drosophila proprioceptors. [FBrf0180120] Urban et al., 2004, Development 131(8): 1835--1845 EGF receptor signalling protects smooth-cuticle cells from apoptosis during Drosophila ventral epidermis development. [FBrf0174566] Duchek and Rorth, 2001, Science 291(5501): 131--133 Guidance of cell migration by EGF receptor signaling during Drosophila oogenesis. [FBrf0132450] Palsson and Gibson, 2000, Dev. Genes Evol. 210(12): 617--622 Quantitative developmental genetic analysis reveals that the ancestral dipteran wing vein prepattern is conserved in Drosophila melanogaster. [FBrf0132291] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Eggert et al., 1998, Genetics 149(3): 1427--1434 Molecular screening for P-element insertions in a large genomic region of Drosophila melanogaster using polymerase chain reaction mediated by the vectorette. [FBrf0102918] Szuts et al., 1998, Genes Dev. 12(13): 2022--2035 Functional intertwining of dpp and EGFR signaling during Drosophila endoderm induction. [FBrf0103047] Wasserman and Freeman, 1998, Cell 95(3): 355--364 An autoregulatory cascade of EGF receptor signaling patterns the Drosophila egg. [FBrf0105339] Yarnitzky et al., 1998, Mech. Dev. 79(1,2): 73--82 An interplay between two EGF-receptor ligands, Vein and Spitz, is required for the formation of a subset of muscle precursors in Drosophila. [FBrf0106043] Yarnitzky et al., 1997, Genes Dev. 11(20): 2691--2700 The Drosophila neuregulin homolog vein mediates inductive interactions between myotubes and their epidermal attachment cells. [FBrf0098912]
Personal communication to FlyBase	Beaton, 1999.12.12, Alleles of the lines in the P-element paper. Alleles of the lines in the P-element paper. [FBrf0125032] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]

Allele Dmel\vn10567

Allele Dmel\vn¹⁰⁵⁶⁷