FlyBase Allele Report: Dmel\vn[10567]

(Jiang and Edgar, 2009, Urban et al., 2004, Spradling et al., 1999, Eggert et al., 1998, Yarnitzky et al., 1998, Yarnitzky et al., 1997, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | larval stage (with vn^γ7)

(Jiang and Edgar, 2009)

decreased cell number | late third instar larval stage | recessive

(Jiang and Edgar, 2009)

lethal | recessive

(Spradling et al., 1999)

lethal - all die before end of P-stage (with vn^γ7)

(Jiang and Edgar, 2009)

some die during pharate adult stage (with vn^γ7)

(Jiang and Edgar, 2009)

Phenotype Manifest In

adult midgut progenitor cell | larval stage (with vn^γ7)

(Jiang and Edgar, 2009)

adult midgut progenitor cell | late third instar larval stage

(Jiang and Edgar, 2009)

dorsal oblique muscle cell

(Yarnitzky et al., 1997)

embryonic/larval midgut | embryonic stage

(Szuts et al., 1998)

embryonic midgut constriction 1

(Szuts et al., 1998)

embryonic midgut constriction 2

(Szuts et al., 1998)

embryonic midgut constriction 3

(Szuts et al., 1998)

gastric caecum

(Szuts et al., 1998)

larval somatic muscle cell

(Yarnitzky et al., 1998)

lch5 ligament attachment cell (with Df(3L)v65c)

(Inbal et al., 2004)

muscle cell of abdominal 2 dorsal acute muscle 1 | precursor

(Yarnitzky et al., 1998)

muscle cell of abdominal 3 dorsal acute muscle 1 | precursor

(Yarnitzky et al., 1998)

muscle cell of abdominal 4 dorsal acute muscle 1 | precursor

(Yarnitzky et al., 1998)

muscle cell of abdominal 5 dorsal acute muscle 1 | precursor

(Yarnitzky et al., 1998)

muscle cell of abdominal 6 dorsal acute muscle 1 | precursor

(Yarnitzky et al., 1998)

muscle cell of abdominal 7 dorsal acute muscle 1 | precursor

(Yarnitzky et al., 1998)

ventral longitudinal muscle cell

(Yarnitzky et al., 1997)

ventral oblique muscle cell

(Yarnitzky et al., 1997)

Detailed Description

Statement

Reference

Whereas wild-type adult midgut progenitor cells (AMPs) form many large clusters, few AMP clusters are found in late larval vn¹⁰⁵⁶⁷ and vn¹⁰⁵⁶⁷/vn^γ7 mutant midguts.

Most vn¹⁰⁵⁶⁷/vn^γ7 mutants die as pharate adults.

Lineage analysis of marked clones reveals that vn¹⁰⁵⁶⁷/vn^γ7 mutants display a failure of proliferation of AMPs during early larval stages, although the number of AMPs generated initially during embryogenesis is not different between mutants and wild-type controls. The size of the few remaining AMP clusters in vn¹⁰⁵⁶⁷/vn^γ7 mutants is relatively normal, suggesting that the late phase of AMP proliferation is largely unaffected in these mutants.

Expression of vn^Scer\UAS.cSa in larval enterocytes driven by Scer\GAL4^{Myo31DF-NP0001} not only rescues the adult midgut progenitor cell phenotype of vn¹⁰⁵⁶⁷ mutants, but it also causes ectopic AMP cell proliferation.

(Jiang and Edgar, 2009)

Late stage vn¹⁰⁵⁶⁷/Df(3L)v65c embryos lack lch5 ligament attachment cells.

(Inbal et al., 2004)

Border cells still migrate dorsally when all dorsal follicle cells are mutant for vn¹⁰⁵⁶⁷ in females containing homozygous clones.

(Duchek and Rorth, 2001)

Shows very mild midgut phenotype. vn¹⁰⁵⁶⁷/Df(3L)γ3 causes lack of midgut constrictions and failure of elongation of gastric caeca.

(Szuts et al., 1998)

A significant number of somatic muscle fibres show multiple, mis-guided membrane extensions at their leading edges. The DA1 muscle precursor is sometimes missing.

(Yarnitzky et al., 1998)

Abnormal muscle phenotype. Myotubes of mutant embryos are elongated and continuously send filopodia in random directions.

(Yarnitzky et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

vn[+]/vn¹⁰⁵⁶⁷ is an enhancer of visible phenotype of Scer\GAL4^ey.PH, brm^{K804R.UAS.Tag:HA}

(Armstrong et al., 2005)

Suppressor of

Statement

Reference

vn¹⁰⁵⁶⁷/vn¹ is a suppressor of increased occurrence of cell division | adult stage phenotype of rk¹

(Scopelliti et al., 2014)

vn[+]/vn¹⁰⁵⁶⁷ is a suppressor of increased cell number phenotype of rk¹

(Scopelliti et al., 2014)

vn[+]/vn¹⁰⁵⁶⁷ is a suppressor of increased occurrence of cell division | adult stage phenotype of rk¹

(Scopelliti et al., 2014)

vn[+]/vn¹⁰⁵⁶⁷ is a suppressor of visible phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

NOT Suppressor of

Statement

Reference

vn[+]/vn¹⁰⁵⁶⁷ is a non-suppressor of visible phenotype of rk¹

(Scopelliti et al., 2014)

Other

Statement

Reference

brm², vn[+]/vn¹⁰⁵⁶⁷ has visible | dominant phenotype

(Armstrong et al., 2005)

brm[+]/brm², vn¹⁰⁵⁶⁷ has visible phenotype

(Armstrong et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

vn¹⁰⁵⁶⁷ has muscle cell of abdominal 2 dorsal acute muscle 1 | precursor phenotype, enhanceable by Scer\GAL4^how-24B/Egfr^DN.UAS

(Yarnitzky et al., 1998)

vn¹⁰⁵⁶⁷ has muscle cell of abdominal 3 dorsal acute muscle 1 | precursor phenotype, enhanceable by Scer\GAL4^how-24B/Egfr^DN.UAS

(Yarnitzky et al., 1998)

vn¹⁰⁵⁶⁷ has muscle cell of abdominal 6 dorsal acute muscle 1 | precursor phenotype, enhanceable by spi³

(Yarnitzky et al., 1998)

vn¹⁰⁵⁶⁷ has muscle cell of abdominal 7 dorsal acute muscle 1 | precursor phenotype, enhanceable by spi³

(Yarnitzky et al., 1998)

vn¹⁰⁵⁶⁷ has muscle cell of abdominal 4 dorsal acute muscle 1 | precursor phenotype, enhanceable by Scer\GAL4^how-24B/Egfr^DN.UAS

(Yarnitzky et al., 1998)

vn¹⁰⁵⁶⁷ has muscle cell of abdominal 5 dorsal acute muscle 1 | precursor phenotype, enhanceable by Scer\GAL4^how-24B/Egfr^DN.UAS

(Yarnitzky et al., 1998)

vn¹⁰⁵⁶⁷ has muscle cell of abdominal 6 dorsal acute muscle 1 | precursor phenotype, enhanceable by Scer\GAL4^how-24B/Egfr^DN.UAS

(Yarnitzky et al., 1998)

vn¹⁰⁵⁶⁷ has muscle cell of abdominal 7 dorsal acute muscle 1 | precursor phenotype, enhanceable by Scer\GAL4^how-24B/Egfr^DN.UAS

(Yarnitzky et al., 1998)

vn¹⁰⁵⁶⁷ has muscle cell of abdominal 2 dorsal acute muscle 1 | precursor phenotype, enhanceable by spi³

(Yarnitzky et al., 1998)

vn¹⁰⁵⁶⁷ has muscle cell of abdominal 3 dorsal acute muscle 1 | precursor phenotype, enhanceable by spi³

(Yarnitzky et al., 1998)

vn¹⁰⁵⁶⁷ has muscle cell of abdominal 4 dorsal acute muscle 1 | precursor phenotype, enhanceable by spi³

(Yarnitzky et al., 1998)

vn¹⁰⁵⁶⁷ has muscle cell of abdominal 5 dorsal acute muscle 1 | precursor phenotype, enhanceable by spi³

(Yarnitzky et al., 1998)

Enhancer of

Statement

Reference

vn[+]/vn¹⁰⁵⁶⁷ is an enhancer of eye phenotype of Scer\GAL4^ey.PH, brm^{K804R.UAS.Tag:HA}

(Armstrong et al., 2005)

vn[+]/vn¹⁰⁵⁶⁷ is an enhancer of ommatidium phenotype of Scer\GAL4^ey.PH, brm^{K804R.UAS.Tag:HA}

(Armstrong et al., 2005)

vn¹⁰⁵⁶⁷/vn¹⁰⁵⁶⁷ is an enhancer of embryonic/first instar larval cuticle phenotype of rho^unspecified

(Urban et al., 2004)

vn¹⁰⁵⁶⁷/vn¹⁰⁵⁶⁷ is an enhancer of ventral denticle belt phenotype of rho^unspecified

(Urban et al., 2004)

vn¹⁰⁵⁶⁷ is an enhancer of dorsal appendage phenotype of Ras85D^ix12a

(Wasserman and Freeman, 1998)

vn¹⁰⁵⁶⁷ is an enhancer of egg phenotype of Ras85D^ix12a

(Wasserman and Freeman, 1998)

Suppressor of

Statement

Reference

vn[+]/vn¹⁰⁵⁶⁷ is a suppressor of intestinal stem cell phenotype of rk¹

(Scopelliti et al., 2014)

vn[+]/vn¹⁰⁵⁶⁷ is a suppressor of adult midgut phenotype of rk¹

(Scopelliti et al., 2014)

vn¹⁰⁵⁶⁷/vn¹ is a suppressor of intestinal stem cell phenotype of rk¹

(Scopelliti et al., 2014)

vn¹⁰⁵⁶⁷/vn¹ is a suppressor of adult midgut phenotype of rk¹

(Scopelliti et al., 2014)

vn[+]/vn¹⁰⁵⁶⁷ is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

NOT Suppressor of

Statement

Reference

vn[+]/vn¹⁰⁵⁶⁷ is a non-suppressor of wing phenotype of rk¹

(Scopelliti et al., 2014)

Other

Statement

Reference

Gug¹⁴⁹⁶⁷, rho^PΔ5, vn¹⁰⁵⁶⁷ has wing vein | somatic clone phenotype

(Charroux et al., 2006)

brm², vn[+]/vn¹⁰⁵⁶⁷ has humeral bristle phenotype

(Armstrong et al., 2005)

brm², vn¹⁰⁵⁶⁷ has humeral bristle phenotype

(Armstrong et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

vn¹⁰⁵⁶⁷/+ partially suppresses the intestinal stem cell hyperproliferation seen in the midgut, but fails to rescue the wing inflation defect of rk¹/rk¹ mutants.

vn¹⁰⁵⁶⁷/vn¹ suppresses the intestinal stem cell hyperproliferation seen in rk¹/rk¹ mutant midguts.

(Scopelliti et al., 2014)

Although extra wing vein material is induced in Gug¹⁴⁹⁶⁷ clones, wing vein differentiation is blocked in vn¹⁰⁵⁶⁷ rho^PΔ5 Gug¹⁴⁹⁶⁷ triple mutant clones.

(Charroux et al., 2006)

2/3 of vn¹⁰⁵⁶⁷; rho^unspecified double homozygous embryo cuticles show a general loss of cuticle integrity. Of the remaining 1/3 of cuticles the percentage with >2 denticle belt fusions is increased from <10% for rho^unspecified to >30%. (Note, while the authors do not name an rho allele for this analysis, they do claim to have used a null allele.)

(Urban et al., 2004)

The loss of the DA1 muscle precursor in vn¹⁰⁵⁶⁷ embryos is enhanced if they are also carrying Egfr^DN.Scer\UAS expressed under the control of Scer\GAL4^how-24B. The loss of the DA1 muscle precursor seen in vn¹⁰⁵⁶⁷ embryos is dominantly enhanced by spi³.

(Yarnitzky et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

vn^rF264

(BDGP Project Members, 1994-1999)

Rescued by

vn¹⁰⁵⁶⁷ is rescued by Scer\GAL4^NP0001/vn^UAS.cSa

(Jiang and Edgar, 2009)

vn¹⁰⁵⁶⁷ is rescued by Scer\GAL4^NP7397/vn^UAS.cSa

(Jiang and Edgar, 2009)

vn¹⁰⁵⁶⁷ is rescued by Scer\GAL4^how-24B/vn^UAS.cSa

(Jiang and Edgar, 2009)

Comments

Expression of vn^Scer\UAS.cSa in adult midgut progenitor cells (AMPs) rescues the AMP cell proliferation phenotype of vn¹⁰⁵⁶⁷ mutants.

Expression of vn^Scer\UAS.cSa in visceral muscle driven by Scer\GAL4^how-24B rescues the adult midgut progenitor cell phenotype of vn¹⁰⁵⁶⁷ mutants.

(Jiang and Edgar, 2009)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11749

cn¹; P{PZ}vn¹⁰⁵⁶⁷ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: Bre1⁰¹⁶⁴⁰. Complements: l(3)02331⁰²³³¹. Complements: l(3)05143⁰⁵¹⁴³. Complements: l(3)neo11¹.

(BDGP Project Members, 1994-1999)

Precise excision of the P{PZ} insertion completely reverses the muscle phenotype and results in full viability. Results indicate the insertion is responsible for the phenotype.

(Yarnitzky et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

P1749

(Yarnitzky et al., 1997)

l(3)10567

(Spradling et al., 1999)

l(3)10567¹⁰⁵⁶⁷

l(3)vn10567

(Jin et al., 2005)

vn¹⁰⁵⁶⁷

(Zhu et al., 2017, Scopelliti et al., 2014, Benito-Sipos et al., 2010, Charroux et al., 2006, Oishi et al., 2006)

vn^P1749

(Jiang and Edgar, 2009, Inbal et al., 2004, Szuts et al., 1998, Yarnitzky et al., 1998)

Name Synonyms

Secondary FlyBase IDs

References (21)

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