Allele Dmel\Prosβ6¹

General Information
Symbol	Dmel\Prosβ6¹	Species	D. melanogaster
Name		FlyBase ID	FBal0010021
Feature type	allele	Associated gene	Dmel\Prosβ6
Also Known As	l(3)73Ai¹, DTS5, Pros26¹

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Holden and Suzuki, 1973)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3L:16,603,204..16,603,204 reported_pr_change=T47I comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental pr_change=T47I\|Pros26-PA na_change=C16603204T (Smyth and Belote, 1999)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name U00790 AAC46465

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: T18I. T18I is also referred to as T47I when the prosequence is included in the amino acid numbering system. (Smyth and Belote, 1999)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| dominant \| heat sensitive (Belote et al., 1990, Schweisguth, 1999, Donaldson et al., 2004, Neuburger et al., 2006) lethal \| dominant \| pupal stage \| heat sensitive (Holden and Suzuki, 1973, Smyth and Belote, 1999) lethal \| larval stage (with Prosβ6^rv10e) (Neuburger et al., 2006) lethal \| larval stage \| recessive (Huang et al., 1995) lethal \| recessive (Neuburger et al., 2006) visible \| dominant \| heat sensitive (Donaldson et al., 2004)
Phenotype Manifest In
	adult abdomen (Smyth and Belote, 1999) adult head (Smyth and Belote, 1999) eo neuron (Schweisguth, 1999, Tang et al., 2005) eo sensory structure (Tang et al., 2005) imaginal disc (Smyth and Belote, 1999) macrochaeta (Schweisguth, 1999, Tang et al., 2005) microchaeta (Schweisguth, 1999) socket (Tang et al., 2005) thecogen cell \| ectopic (Schweisguth, 1999) tormogen cell \| ectopic (Schweisguth, 1999) trichogen cell (Schweisguth, 1999) wing vein \| ectopic \| heat sensitive (Donaldson et al., 2004)
Detailed Description
	Statement Reference A Pros26[1] background suppresses the cell division disruption found in CoVa[tend] mutant eye disc clones. Interestingly, the glossy adult eye phenotype of CoVa[tend] mutant clones is also significantly suppressed. (Mandal et al., 2010) Pros26¹/Pros26^rv10e animals show early larval lethality at 25^oC. Homozygotes are lethal at 25^oC. Brains of heterozygous larvae raised at the restrictive temperature of 29^oC show no apparent polyploidy or aneuploidy and chromosome morphology and anaphase figures appear normal. The mean mitotic index is somewhat reduced compared to controls, probably reflecting the slower developmental rate of the mutant larvae. (Neuburger et al., 2006) Pros26[1] mutants exhibit cell fate changes in the SOP lineage leading to mutant external sensory organs with two to four sockets, or two hairs and two sockets, accompanied by an absence of neurons. (Tang et al., 2005) Heterozygotes shifted to the restrictive temperature for 48 hours during the third larval instar survive to adulthood and have a mild extra vein phenotype in the wing. (Donaldson et al., 2004) Expression of Pros26¹ under the control of Scer\GAL4^GMR.PF does not result in a rough eye phenotype. (Shulman and Feany, 2003) Heterozygous pupae heat pulsed between 7 and 23 hour APF give rise to pharate adults that do not emerge but show specific bristle defects. A small number of double socket sense organs appear on the notum, abdomen and wing margin. Sheath cells are occasionally duplicated. The shaft cell is occasionally transformed into a second socket cell. pros expression indicates that microchaetae occasionally show a neuron to sheath transformation. Longer heat pulses cause earlier lethality. (Schweisguth, 1999) Heterozygotes die during the late pupal stage when raised at 29^oC. The pupae have reduced imaginal disc derivatives, often show failure of head eversion and lack adult abdomen structures. (Smyth and Belote, 1999) Heterozygous individuals die at late pupal stage in cultures maintained at 29^oC. Characteristic abnormality is poorly developed or degenerate abdomens. Heterozygotes are wild type phenotype at ambient temperatures around 25^oC. (Belote et al., 1990) Viable when hemizygous at 18^oC, but the flies have thickened L2 wing veins. (Ashburner et al., 1981) Heterozygotes die as pupae at 29^oC. Within the pupal case the anterior portions of the adult are fully differentiated but the abdominal region fails to develop and histolyzes. Prosβ2¹ Pros26¹ flies do not survive at 17 or 22^oC. The temperature-sensitive period begins during the third larval instar stage and ends with puparium formation, lasting approximately 24 hours. (Holden and Suzuki, 1973) dominant temperature-sensitive lethal larval/pupal lethal hfw-like dominant temperature-sensitive
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Prosβ2¹, Pros26[+]/Prosβ6¹ has lethal \| dominant \| larval stage phenotype, suppressible \| partially by Prosα2^SuDTS/Su(DTS)-1[+] (Neuburger et al., 2006) Prosβ2¹, Prosβ6¹ has lethal \| dominant \| larval stage phenotype, suppressible \| partially by Prosα2^SuDTS/Su(DTS)-1[+] (Smyth and Belote, 1999) Prosβ2¹/Prosbeta2[+], Prosβ6¹ has lethal \| dominant \| larval stage phenotype, suppressible \| partially by Prosα2^SuDTS/Su(DTS)-1[+] (Neuburger et al., 2006) Prosβ6¹ has lethal \| dominant \| heat sensitive phenotype, suppressible \| partially by Df(3R)T-32/+ (Neuburger et al., 2006) Prosβ6¹ has lethal \| dominant \| heat sensitive phenotype, suppressible by Prosα2^SuDTS/Su(DTS)-1[+] (Neuburger et al., 2006) Prosβ6¹ has lethal \| recessive phenotype, suppressible \| partially by Prosα2^SuDTS/Su(DTS)-1[+] (Neuburger et al., 2006)
Enhancer of
Statement Reference Pros26[+]/Prosβ6¹ is an enhancer of lethal \| partially \| prepupal stage phenotype of not¹/Df(3L)W4 (Poeck et al., 2001) Prosβ6¹ is an enhancer \| heat sensitive of visible phenotype of Scer\GAL4[-], vn^Scer\UAS.cSa (Donaldson et al., 2004) Prosβ6¹ is an enhancer of neuroanatomy defective phenotype of Hsap\HTT^GMR.Q120 (Kanuka et al., 2003)
NOT Enhancer of
Statement Reference Prosβ6¹ is a non-enhancer of visible phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Shulman and Feany, 2003)
Suppressor of
Statement Reference Pros26[+]/Prosβ6¹ is a suppressor \| heat sensitive \| partially of short lived phenotype of Tpi^sgk-1/Tpi^sgk-1 (Seigle et al., 2008) Pros26[+]/Prosβ6¹ is a suppressor \| partially of lethal \| recessive phenotype of Prosα2^SuDTS (Neuburger et al., 2006) Pros26[+]/Prosβ6¹ is a suppressor of mitotic cell cycle defective \| somatic clone phenotype of CoVa^tend (Mandal et al., 2010) Prosβ6¹ is a suppressor of visible \| recessive phenotype of faf^FO8/faf^BX3, lqf^bE25 (Cadavid et al., 2000) Prosβ6¹ is a suppressor of visible phenotype of faf^FO8/faf^BX3, lqf^bE25 (Cadavid et al., 2000)
NOT Suppressor of
Statement Reference Prosβ6¹ is a non-suppressor of neurophysiology defective phenotype of Cerk¹ (Dasgupta et al., 2009) Prosβ6¹ is a non-suppressor of visible phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Shulman and Feany, 2003)
Other
Statement Reference Prosα2^SuDTS/Su(DTS)-1[+], Prosβ6¹ has viable phenotype (Neuburger et al., 2006) Prosβ2¹, Pros26[+]/Prosβ6¹ has lethal \| dominant \| larval stage phenotype (Neuburger et al., 2006) Prosβ2¹, Prosβ6¹ has lethal \| dominant \| larval stage phenotype (Smyth and Belote, 1999, Smyth and Belote, 1999) Prosβ2¹/Prosbeta2[+], Prosβ6¹ has lethal \| dominant \| larval stage phenotype (Neuburger et al., 2006)
Phenotype Manifest In
Enhanced by
Statement Reference Prosβ6¹ has macrochaeta phenotype, enhanceable by H[+]/H^E31 (Schweisguth, 1999) Prosβ6¹ has microchaeta phenotype, enhanceable by H[+]/H^E31 (Schweisguth, 1999)
Suppressed by
Statement Reference Prosβ6¹ has eo neuron phenotype, suppressible by numb^{ΔC.Scer\UAS.T:Hsap\MYC}/Scer\GAL4^sca-537.4 (Tang et al., 2005) Prosβ6¹ has eo sensory structure phenotype, suppressible by numb^{ΔC.Scer\UAS.T:Hsap\MYC}/Scer\GAL4^sca-537.4 (Tang et al., 2005) Prosβ6¹ has macrochaeta phenotype, suppressible by numb^{ΔC.Scer\UAS.T:Hsap\MYC}/Scer\GAL4^sca-537.4 (Tang et al., 2005) Prosβ6¹ has socket phenotype, suppressible by numb^{ΔC.Scer\UAS.T:Hsap\MYC}/Scer\GAL4^sca-537.4 (Tang et al., 2005)
Enhancer of
Statement Reference Pros26[+]/Prosβ6¹ is an enhancer of macrochaeta phenotype of H^E31 (Schweisguth, 1999) Pros26[+]/Prosβ6¹ is an enhancer of microchaeta phenotype of H^E31 (Schweisguth, 1999) Pros26[+]/Prosβ6¹ is an enhancer of phenotype of rpr^GMR.PW (Ryoo et al., 2002) Pros26[+]/Prosβ6¹ is an enhancer of photoreceptor cell & axon phenotype of not¹/Df(3L)W4 (Poeck et al., 2001) Prosβ6¹ is an enhancer \| heat sensitive of wing vein \| ectopic phenotype of Scer\GAL4[-], vn^Scer\UAS.cSa (Donaldson et al., 2004) Prosβ6¹ is an enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Kanuka et al., 2003) Prosβ6¹ is an enhancer of eye phenotype of Hsap\HTT^GMR.Q120 (Kanuka et al., 2003) Prosβ6¹ is an enhancer of macrochaeta phenotype of N^icd.hs (Schweisguth, 1999) Prosβ6¹ is an enhancer of ommatidium phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Kanuka et al., 2003) Prosβ6¹ is an enhancer of tormogen cell \| ectopic phenotype of N^icd.hs (Schweisguth, 1999)
NOT Enhancer of
Statement Reference Pros26[+]/Prosβ6¹ is a non-enhancer of phenotype of W^GMR.PG/W^GMR.PG (Ryoo et al., 2002) Prosβ6¹ is a non-enhancer of eye phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Shulman and Feany, 2003)
Suppressor of
Statement Reference Pros26[+]/Prosβ6¹ is a suppressor of eye \| somatic clone phenotype of CoVa^tend (Mandal et al., 2010) Prosβ6¹ is a suppressor of eye phenotype of faf^FO8 (Huang et al., 1995) Prosβ6¹ is a suppressor of ommatidium phenotype of faf^FO8/faf^BX3, lqf^bE25 (Cadavid et al., 2000)
NOT Suppressor of
Statement Reference Prosβ6¹ is a non-suppressor of eye phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Shulman and Feany, 2003) Prosβ6¹ is a non-suppressor of eye photoreceptor cell phenotype of Cerk¹ (Dasgupta et al., 2009) Prosβ6¹ is a non-suppressor of rhabdomere phenotype of Cerk¹ (Dasgupta et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference Pros26[1] cannot rescue the lack of a light-induced electroretinogram response and the retinal degeneration that is seen in Cerk[1] flies. (Dasgupta et al., 2009) Pros26[1]/+ can partially suppress the reduced median lifespan of Tpi[sgk-1] homozygous flies when the double mutants are raised at 29[o]C. (Seigle et al., 2008) Pros26¹ Pros25^SuDTS double heterozygotes are viable at 29^oC, in contrast to Pros26¹ single heterozygotes. The recessive lethality of Pros26¹ homozygotes seen at 25^oC is only partially suppressed by Pros25^SuDTS/+ and the survivors are slow developing and sick. Pros26¹ Prosβ2¹ double heterozygotes show early larval lethality at 25^oC, which is partially suppressed by Pros25^SuDTS/+. Pros26¹/+ can weakly suppress the lethality of Pros25^SuDTS homozygotes at 25^oC. (Neuburger et al., 2006) The multi-socket phenotype found in Pros26[1] mutants is completely suppressed by expression of numb[ΔC.Scer\UAS.T:Hsap\MYC] (under the control of Scer\GAL4[sca-537.4]) with the resultant flies exhibiting only the numb phenotype, namely, balding of the notum. Underneath the balding patches, there are clusters of as many as four neurons. (Tang et al., 2005) The constitutive extra vein phenotype in the wing caused by the P{UAS-vn.S}1.1 insertion in the absence of an Scer\GAL4 driver is enhanced in combination with Pros26¹ and following a shift to the restrictive temperature. (Donaldson et al., 2004) The prepupal lethality and photoreceptor axon targeting phenotype of not¹/Df(3L)W4 animals is enhanced by one copy of Pros26¹; 78.5% of animals show prepupal lethality and 69.4% show a strong "nonstop" phenotype (more than 50% of photoreceptor cell axon bundles pass the lamina and hyperinnervate the medulla). (Poeck et al., 2001) The lqf^bE25 faf^BX3/faf^FO8 mutant phenotype is suppressed by Pros26¹. (Cadavid et al., 2000) Double heterozygotes of H^E31 with Pros26¹ or Prosβ2¹ show strong synergistic enhancement of the double socket bristle and microchaete phenotypes. The mild double socket phenotype of N^icd.hs is enhanced by Pros26¹ and Prosβ2¹. The N^icd.hs gene product is more stable in Pros26¹ expressing cells. (Schweisguth, 1999) The Pros26¹ dominant temperature sensitive lethal effect is dominantly suppressed by Su(DTS)-1¹. Pros26¹ and Prosβ2¹ are lethal in transheterozygous condition, even at normally permissive temperatures. The synthetic lethal period occurs during early larval development, in contrast to the dominant temperature sensitive lethal period of either allele which occurs during the late pupal stage. This synthetic lethal effect is partially suppressed by Su(DTS)-1¹; a small fraction of + Pros26¹ Su(DTS)-1¹/Prosβ2¹ + + animals survive to adulthood at 18^oC. Does not show a dominant synthetic lethal interaction with Prosβ2^rv19X, Prosβ2^rv3γ, Prosβ2^rv11γ, Prosβ2^rv12γ or Prosβ2^rv3D. (Smyth and Belote, 1999) Heterozygosity for Pros26¹ dramatically reduces the severity of the eye phenotype of faf^FO8 homozygotes. (Huang et al., 1995)
Xenogenetic Interactions
Statement Reference The rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is not modified if the flies are also carrying Pros26¹. (Shulman and Feany, 2003)
Complementation & Rescue Data
Rescued by	Prosβ6¹ is rescued by Prosβ6^+t3.5 (Saville and Belote, 1993)
Comments
Stocks ( 2 )
Bloomington	6182 Prosβ6¹ pb¹ p^p/TM3, Sb¹ Ser¹
Kyoto	108671 Prosβ6¹ pb¹ p^p/TM3, Sb¹ Ser¹
Notes on Origin
Discoverer	Suzuki.

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	DTS5 (Donaldson et al., 2004, Schweisguth, 1999, Warrick et al., 2005, Dasgupta et al., 2009, Dasgupta et al., 2009) DTS-5 (Holden and Suzuki, 1973) l(3)73Ai¹ (Poeck et al., 2001, Cadavid et al., 2000, Smyth and Belote, 1999, Huang et al., 1995, Saville and Belote, 1993, Mandal et al., 2010) l(3)DTS-5 (Ashburner et al., 1981) l(3)DTS5 Pros26¹ (Neuburger et al., 2006, Seigle et al., 2008) Pros¹ (Shulman and Feany, 2003) Prosβ6¹
Name Synonym
Secondary FlyBase IDs

References ( 22 )

Generate a list of	All references relating to this allele ( 22 )

List References by type	Research paper ( 21 ) Supplementary material ( 1 )
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Allele Dmel\Prosβ61

Allele Dmel\Prosβ6¹