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General Information
Symbol
Dmel\Prosβ61
Species
D. melanogaster
Name
FlyBase ID
FBal0010021
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
DTS5, l(3)73Ai1, Pros261
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C16610104T

Amino acid change:

T47I | Prosbeta6-PA

Reported amino acid change:

T47I

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: T18I. T18I is also referred to as T47I when the prosequence is included in the amino acid numbering system.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Prosβ61 heterozygote adult females kept at a non-permissive temperature (29[o]C) do not display any defects in the number of cytoophidia (CTPsyn filaments) in the egg chambers.

A Pros261 background suppresses the cell division disruption found in CoVatend mutant eye disc clones. Interestingly, the glossy adult eye phenotype of CoVatend mutant clones is also significantly suppressed.

Pros261/Pros26rv10e animals show early larval lethality at 25oC.

Homozygotes are lethal at 25oC.

Brains of heterozygous larvae raised at the restrictive temperature of 29oC show no apparent polyploidy or aneuploidy and chromosome morphology and anaphase figures appear normal. The mean mitotic index is somewhat reduced compared to controls, probably reflecting the slower developmental rate of the mutant larvae.

Pros261 mutants exhibit cell fate changes in the SOP lineage leading to mutant external sensory organs with two to four sockets, or two hairs and two sockets, accompanied by an absence of neurons.

Heterozygotes shifted to the restrictive temperature for 48 hours during the third larval instar survive to adulthood and have a mild extra vein phenotype in the wing.

Expression of Pros261 under the control of Scer\GAL4GMR.PF does not result in a rough eye phenotype.

Heterozygous pupae heat pulsed between 7 and 23 hour APF give rise to pharate adults that do not emerge but show specific bristle defects. A small number of double socket sense organs appear on the notum, abdomen and wing margin. Sheath cells are occasionally duplicated. The shaft cell is occasionally transformed into a second socket cell. pros expression indicates that microchaetae occasionally show a neuron to sheath transformation. Longer heat pulses cause earlier lethality.

Heterozygotes die during the late pupal stage when raised at 29oC. The pupae have reduced imaginal disc derivatives, often show failure of head eversion and lack adult abdomen structures.

Heterozygous individuals die at late pupal stage in cultures maintained at 29oC. Characteristic abnormality is poorly developed or degenerate abdomens. Heterozygotes are wild type phenotype at ambient temperatures around 25oC.

Viable when hemizygous at 18oC, but the flies have thickened L2 wing veins.

Heterozygotes die as pupae at 29oC. Within the pupal case the anterior portions of the adult are fully differentiated but the abdominal region fails to develop and histolyzes. Prosβ21 Pros261 flies do not survive at 17 or 22oC. The temperature-sensitive period begins during the third larval instar stage and ends with puparium formation, lasting approximately 24 hours.

dominant temperature-sensitive lethal larval/pupal lethal hfw-like dominant temperature-sensitive

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

Prosβ61 has lethal | dominant | heat sensitive phenotype, suppressible | partially by Df(3R)T-32/+

Prosβ21, Pros26[+]/Prosβ61 has lethal | dominant | larval stage phenotype, suppressible | partially by Prosα2SuDTS/Su(DTS)-1[+]

Prosβ61 has lethal | dominant | heat sensitive phenotype, suppressible by Prosα2SuDTS/Su(DTS)-1[+]

Prosβ61 has lethal | recessive phenotype, suppressible | partially by Prosα2SuDTS/Su(DTS)-1[+]

Prosβ21/Prosbeta2[+], Prosβ61 has lethal | dominant | larval stage phenotype, suppressible | partially by Prosα2SuDTS/Su(DTS)-1[+]

Prosβ21, Prosβ61 has lethal | dominant | larval stage phenotype, suppressible | partially by Prosα2SuDTS/Su(DTS)-1[+]

Enhancer of
Statement
Reference

Prosβ61 is an enhancer | heat sensitive of visible phenotype of Scer\GAL4[-], vnUAS.cSa

Prosbeta6[+]/Prosβ61 is an enhancer of some die during P-stage phenotype of not1/Df(3L)W4

NOT Enhancer of
Suppressor of
Statement
Reference

Pros26[+]/Prosβ61 is a suppressor | heat sensitive | partially of short lived phenotype of Tpisgk-1/Tpisgk-1

Pros26[+]/Prosβ61 is a suppressor | partially of lethal | recessive phenotype of Prosα2SuDTS

Prosβ61 is a suppressor of visible phenotype of fafFO8/fafBX3, lqfbE25

Prosβ61 is a suppressor of visible | recessive phenotype of fafFO8/fafBX3, lqfbE25

NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Prosβ61 has macrochaeta phenotype, enhanceable by H[+]/HE31

Prosβ61 has microchaeta phenotype, enhanceable by H[+]/HE31

Suppressed by
Enhancer of
Statement
Reference

Prosβ61 is an enhancer | heat sensitive of wing vein | ectopic phenotype of Scer\GAL4[-], vnUAS.cSa

Prosβ61 is an enhancer of eye phenotype of Hsap\HTTGMR.Q120

Pros26[+]/Prosβ61 is an enhancer of phenotype of rprGMR.PW

Pros26[+]/Prosβ61 is an enhancer of photoreceptor cell & axon phenotype of not1/Df(3L)W4

Pros26[+]/Prosβ61 is an enhancer of macrochaeta phenotype of HE31

Pros26[+]/Prosβ61 is an enhancer of microchaeta phenotype of HE31

Prosβ61 is an enhancer of macrochaeta phenotype of Nicd.hs

Prosβ61 is an enhancer of tormogen cell | ectopic phenotype of Nicd.hs

NOT Enhancer of
Statement
Reference

Pros26[+]/Prosβ61 is a non-enhancer of phenotype of hidGMR.PG/WGMR.PG

Suppressor of
Statement
Reference

Pros26[+]/Prosβ61 is a suppressor of eye | somatic clone phenotype of COX5Atend

Prosβ61 is a suppressor of ommatidium phenotype of fafFO8/fafBX3, lqfbE25

Prosβ61 is a suppressor of eye phenotype of fafFO8

NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

The number of cytoophidia (CTPsyn filaments) in the egg chambers of adult Prosβ61,CblF165 double heterozygote females is significantly reduced compared to either Prosβ61 or CblF165 single heterozygotes.

Pros261 cannot rescue the lack of a light-induced electroretinogram response and the retinal degeneration that is seen in Cerk1 flies.

Pros261/+ can partially suppress the reduced median lifespan of Tpisgk-1 homozygous flies when the double mutants are raised at 29[o]C.

Pros261 Pros25SuDTS double heterozygotes are viable at 29oC, in contrast to Pros261 single heterozygotes. The recessive lethality of Pros261 homozygotes seen at 25oC is only partially suppressed by Pros25SuDTS/+ and the survivors are slow developing and sick.

Pros261 Prosβ21 double heterozygotes show early larval lethality at 25oC, which is partially suppressed by Pros25SuDTS/+.

Pros261/+ can weakly suppress the lethality of Pros25SuDTS homozygotes at 25oC.

The multi-socket phenotype found in Pros261 mutants is completely suppressed by expression of numbΔC.Scer\UAS.T:Hsap\MYC (under the control of Scer\GAL4sca-537.4) with the resultant flies exhibiting only the numb phenotype, namely, balding of the notum. Underneath the balding patches, there are clusters of as many as four neurons.

The constitutive extra vein phenotype in the wing caused by the P{UAS-vn.S}1.1 insertion in the absence of an Scer\GAL4 driver is enhanced in combination with Pros261 and following a shift to the restrictive temperature.

The prepupal lethality and photoreceptor axon targeting phenotype of not1/Df(3L)W4 animals is enhanced by one copy of Pros261; 78.5% of animals show prepupal lethality and 69.4% show a strong "nonstop" phenotype (more than 50% of photoreceptor cell axon bundles pass the lamina and hyperinnervate the medulla).

The lqfbE25 fafBX3/fafFO8 mutant phenotype is suppressed by Pros261.

Double heterozygotes of HE31 with Pros261 or Prosβ21 show strong synergistic enhancement of the double socket bristle and microchaete phenotypes. The mild double socket phenotype of Nicd.hs is enhanced by Pros261 and Prosβ21. The Nicd.hs gene product is more stable in Pros261 expressing cells.

The Pros261 dominant temperature sensitive lethal effect is dominantly suppressed by Su(DTS)-11. Pros261 and Prosβ21 are lethal in transheterozygous condition, even at normally permissive temperatures. The synthetic lethal period occurs during early larval development, in contrast to the dominant temperature sensitive lethal period of either allele which occurs during the late pupal stage. This synthetic lethal effect is partially suppressed by Su(DTS)-11; a small fraction of + Pros261 Su(DTS)-11/Prosβ21 + + animals survive to adulthood at 18oC. Does not show a dominant synthetic lethal interaction with Prosβ2rv19X, Prosβ2rv3γ, Prosβ2rv11γ, Prosβ2rv12γ or Prosβ2rv3D.

Heterozygosity for Pros261 dramatically reduces the severity of the eye phenotype of fafFO8 homozygotes.

Xenogenetic Interactions
Statement
Reference

Prosβ61 does not suppress the protective role of expression of Hsap\ATXN3Scer\UAS.WT.T:Zzzz\His6 or DnaJ-1Scer\UAS.cKa in suppression of eye degeneration in Scer\GAL4GMR.long>Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 flies. Prosβ61 does not alter eye degeneration in Scer\GAL4GMR.long>Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 flies.

Reducing proteasome function by introduction of Prosβ61 to Scer\GAL4Bx-MS1096/Mmus\Cul1Scer\UAS.cHa flies enhances the wing size phenotype.

The rough eye phenotype caused by expression of Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4GMR.PF is not modified if the flies are also carrying Pros261.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Suzuki.

External Crossreferences and Linkouts ( 2 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
Synonyms and Secondary IDs (9)
References (26)