FlyBase Allele Report: Dmel\Prosβ6[1]

General Information

Symbol

Dmel\Prosβ6¹

Species

D. melanogaster

Name

FlyBase ID

FBal0010021

Feature type

allele

Associated gene

Dmel\Prosβ6

Associated Insertion(s)

Carried in Construct

Also Known As

DTS5, l(3)73Ai¹, Pros26¹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Holden and Suzuki, 1973)

Progenitor genotype

Cytology

Description

Amino acid replacement: T18I. T18I is also referred to as T47I when the prosequence is included in the amino acid numbering system.

(Smyth and Belote, 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:16,610,104..16,610,104 [-]

Nucleotide change:

C16610104T

Amino acid change:

T47I | Prosbeta6-PA

Reported amino acid change:

T47I

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Smyth and Belote, 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate

(Neuburger et al., 2006)

decreased occurrence of cell division | larval stage

(Neuburger et al., 2006)

lethal | dominant | heat sensitive

(Neuburger et al., 2006, Donaldson et al., 2004, Schweisguth, 1999, Belote et al., 1990)

lethal | recessive

(Neuburger et al., 2006)

lethal - all die before end of larval stage (with Prosβ6^rv10e)

(Neuburger et al., 2006)

lethal - all die before end of larval stage | recessive

(Huang et al., 1995)

lethal - all die before end of pupal stage | dominant | heat sensitive

(Smyth and Belote, 1999, Holden and Suzuki, 1973)

some die during larval stage (with Prosβ6^rv10e)

(Neuburger et al., 2006)

some die during larval stage | recessive

(Huang et al., 1995)

some die during pupal stage | dominant | heat sensitive

(Smyth and Belote, 1999, Holden and Suzuki, 1973)

visible | dominant | heat sensitive

(Donaldson et al., 2004)

Phenotype Manifest In

Detailed Description

Statement

Reference

Prosβ6¹ heterozygote adult females kept at a non-permissive temperature (29[o]C) do not display any defects in the number of cytoophidia (CTPsyn filaments) in the egg chambers.

(Wang et al., 2015)

A Pros26¹ background suppresses the cell division disruption found in CoVa^tend mutant eye disc clones. Interestingly, the glossy adult eye phenotype of CoVa^tend mutant clones is also significantly suppressed.

(Mandal et al., 2010)

Pros26¹/Pros26^rv10e animals show early larval lethality at 25^oC.

Homozygotes are lethal at 25^oC.

Brains of heterozygous larvae raised at the restrictive temperature of 29^oC show no apparent polyploidy or aneuploidy and chromosome morphology and anaphase figures appear normal. The mean mitotic index is somewhat reduced compared to controls, probably reflecting the slower developmental rate of the mutant larvae.

(Neuburger et al., 2006)

Pros26¹ mutants exhibit cell fate changes in the SOP lineage leading to mutant external sensory organs with two to four sockets, or two hairs and two sockets, accompanied by an absence of neurons.

(Tang et al., 2005)

Heterozygotes shifted to the restrictive temperature for 48 hours during the third larval instar survive to adulthood and have a mild extra vein phenotype in the wing.

(Donaldson et al., 2004)

Expression of Pros26¹ under the control of Scer\GAL4^GMR.PF does not result in a rough eye phenotype.

(Shulman and Feany, 2003)

Heterozygous pupae heat pulsed between 7 and 23 hour APF give rise to pharate adults that do not emerge but show specific bristle defects. A small number of double socket sense organs appear on the notum, abdomen and wing margin. Sheath cells are occasionally duplicated. The shaft cell is occasionally transformed into a second socket cell. pros expression indicates that microchaetae occasionally show a neuron to sheath transformation. Longer heat pulses cause earlier lethality.

(Schweisguth, 1999)

Heterozygotes die during the late pupal stage when raised at 29^oC. The pupae have reduced imaginal disc derivatives, often show failure of head eversion and lack adult abdomen structures.

(Smyth and Belote, 1999)

Heterozygous individuals die at late pupal stage in cultures maintained at 29^oC. Characteristic abnormality is poorly developed or degenerate abdomens. Heterozygotes are wild type phenotype at ambient temperatures around 25^oC.

(Belote et al., 1990)

Viable when hemizygous at 18^oC, but the flies have thickened L2 wing veins.

(Ashburner et al., 1981)

Heterozygotes die as pupae at 29^oC. Within the pupal case the anterior portions of the adult are fully differentiated but the abdominal region fails to develop and histolyzes. Prosβ2¹ Pros26¹ flies do not survive at 17 or 22^oC. The temperature-sensitive period begins during the third larval instar stage and ends with puparium formation, lasting approximately 24 hours.

(Holden and Suzuki, 1973)

dominant temperature-sensitive lethal larval/pupal lethal hfw-like dominant temperature-sensitive

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Prosβ2¹/Prosbeta2[+], Prosβ6¹ has lethal | dominant | larval stage phenotype, suppressible | partially by Prosα2^SuDTS/Su(DTS)-1[+]

(Neuburger et al., 2006)

Prosβ2¹, Pros26[+]/Prosβ6¹ has lethal | dominant | larval stage phenotype, suppressible | partially by Prosα2^SuDTS/Su(DTS)-1[+]

(Neuburger et al., 2006)

Prosβ6¹ has lethal | dominant | heat sensitive phenotype, suppressible by Prosα2^SuDTS/Su(DTS)-1[+]

(Neuburger et al., 2006)

Prosβ6¹ has lethal | recessive phenotype, suppressible by Prosα2^SuDTS/Su(DTS)-1[+]

(Neuburger et al., 2006)

Prosβ6¹ has lethal | dominant | heat sensitive phenotype, suppressible by Df(3R)T-32/+

(Neuburger et al., 2006)

Prosβ2¹, Prosβ6¹ has lethal | dominant | larval stage phenotype, suppressible | partially by Prosα2^SuDTS/Su(DTS)-1[+]

(Smyth and Belote, 1999)

Enhancer of

Statement

Reference

Prosβ6¹ is an enhancer of visible phenotype of vn^UAS.cSa

(Donaldson et al., 2004)

Prosβ6¹ is an enhancer of abnormal neuroanatomy phenotype of Hsap\HTT^GMR.Q120

(Kanuka et al., 2003)

Prosbeta6[+]/Prosβ6¹ is an enhancer of some die during P-stage phenotype of not¹/Df(3L)W4

(Poeck et al., 2001)

NOT Enhancer of

Statement

Reference

Prosβ6¹ is a non-enhancer of visible phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Tsou et al., 2015)

Prosβ6¹ is a non-enhancer of visible phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Suppressor of

Statement

Reference

Prosbeta6[+]/Prosβ6¹ is a suppressor of decreased occurrence of cell division | somatic clone | larval stage phenotype of COX5A^tend

(Mandal et al., 2010)

Pros26[+]/Prosβ6¹ is a suppressor of short lived phenotype of Tpi^sgk-1

(Seigle et al., 2008)

Pros26[+]/Prosβ6¹ is a suppressor of lethal | recessive phenotype of Prosα2^SuDTS

(Neuburger et al., 2006)

Prosβ6¹ is a suppressor of visible | recessive phenotype of faf^FO8/faf^BX3, lqf^bE25

(Cadavid et al., 2000)

Prosβ6¹ is a suppressor of visible phenotype of faf^FO8/faf^BX3, lqf^bE25

(Cadavid et al., 2000)

NOT Suppressor of

Statement

Reference

Prosβ6¹ is a non-suppressor of visible phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Tsou et al., 2015)

Prosβ6¹ is a non-suppressor of abnormal neurophysiology phenotype of Cerk¹

(Dasgupta et al., 2009)

Prosβ6¹ is a non-suppressor of visible phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Other

Statement

Reference

Prosβ2¹, Pros26[+]/Prosβ6¹ has lethal | dominant | larval stage phenotype

(Neuburger et al., 2006)

Prosα2^SuDTS/Su(DTS)-1[+], Prosβ6¹ has viable phenotype

(Neuburger et al., 2006)

Prosβ2¹/Prosbeta2[+], Prosβ6¹ has lethal | dominant | larval stage phenotype

(Neuburger et al., 2006)

Prosβ2¹, Prosβ6¹ has lethal | dominant | larval stage phenotype

(Smyth and Belote, 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

Prosβ6¹ has macrochaeta phenotype, enhanceable by H[+]/H^E31

(Schweisguth, 1999)

Prosβ6¹ has microchaeta phenotype, enhanceable by H[+]/H^E31

(Schweisguth, 1999)

Suppressed by

Statement

Reference

Prosβ6¹ has macrochaeta phenotype, suppressible by numb^{ΔC.UAS.Tag:MYC}/Scer\GAL4^sca-537.4

(Tang et al., 2005)

Prosβ6¹ has eo neuron phenotype, suppressible by numb^{ΔC.UAS.Tag:MYC}/Scer\GAL4^sca-537.4

(Tang et al., 2005)

Prosβ6¹ has tormogen cell phenotype, suppressible by numb^{ΔC.UAS.Tag:MYC}/Scer\GAL4^sca-537.4

(Tang et al., 2005)

Prosβ6¹ has eo sensory structure phenotype, suppressible by numb^{ΔC.UAS.Tag:MYC}/Scer\GAL4^sca-537.4

(Tang et al., 2005)

Enhancer of

Statement

Reference

Prosβ6¹ is an enhancer of wing vein | ectopic phenotype of vn^UAS.cSa

(Donaldson et al., 2004)

Prosβ6¹ is an enhancer of wing phenotype of Mmus\Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096

(Heriche et al., 2003)

Prosβ6¹ is an enhancer of eye phenotype of Hsap\HTT^GMR.Q120

(Kanuka et al., 2003)

Prosβ6¹ is an enhancer of ommatidium phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Kanuka et al., 2003)

Prosβ6¹ is an enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Kanuka et al., 2003)

Pros26[+]/Prosβ6¹ is an enhancer of phenotype of rpr^GMR.PW

(Ryoo et al., 2002)

Pros26[+]/Prosβ6¹ is an enhancer of photoreceptor cell & axon phenotype of not¹/Df(3L)W4

(Poeck et al., 2001)

Pros26[+]/Prosβ6¹ is an enhancer of macrochaeta phenotype of H^E31

(Schweisguth, 1999)

Pros26[+]/Prosβ6¹ is an enhancer of microchaeta phenotype of H^E31

(Schweisguth, 1999)

Prosβ6¹ is an enhancer of macrochaeta phenotype of N^icd.hs

(Schweisguth, 1999)

Prosβ6¹ is an enhancer of tormogen cell | ectopic phenotype of N^icd.hs

(Schweisguth, 1999)

NOT Enhancer of

Statement

Reference

Prosβ6¹ is a non-enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Tsou et al., 2015)

Prosβ6¹ is a non-enhancer of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Pros26[+]/Prosβ6¹ is a non-enhancer of phenotype of hid^GMR.PG

(Ryoo et al., 2002)

Suppressor of

Statement

Reference

Pros26[+]/Prosβ6¹ is a suppressor of eye | somatic clone phenotype of COX5A^tend

(Mandal et al., 2010)

Prosβ6¹ is a suppressor of ommatidium phenotype of faf^FO8/faf^BX3, lqf^bE25

(Cadavid et al., 2000)

Prosβ6¹ is a suppressor of eye phenotype of faf^FO8

(Huang et al., 1995)

NOT Suppressor of

Statement

Reference

Prosβ6¹ is a non-suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Tsou et al., 2015)

Prosβ6¹ is a non-suppressor of rhabdomere phenotype of Cerk¹

(Dasgupta et al., 2009)

Prosβ6¹ is a non-suppressor of eye photoreceptor cell phenotype of Cerk¹

(Dasgupta et al., 2009)

Prosβ6¹ is a non-suppressor of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Other

Statement

Reference

Cbl^F165, Prosβ6¹ has egg chamber phenotype

(Wang et al., 2015)

Cbl^F165, Prosβ6¹ has cytoophidium | female | adult stage phenotype

(Wang et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The number of cytoophidia (CTPsyn filaments) in the egg chambers of adult Prosβ6¹,Cbl^F165 double heterozygote females is significantly reduced compared to either Prosβ6¹ or Cbl^F165 single heterozygotes.

(Wang et al., 2015)

Pros26¹ cannot rescue the lack of a light-induced electroretinogram response and the retinal degeneration that is seen in Cerk¹ flies.

(Dasgupta et al., 2009)

Pros26¹/+ can partially suppress the reduced median lifespan of Tpi^sgk-1 homozygous flies when the double mutants are raised at 29[o]C.

(Seigle et al., 2008)

Pros26¹ Pros25^SuDTS double heterozygotes are viable at 29^oC, in contrast to Pros26¹ single heterozygotes. The recessive lethality of Pros26¹ homozygotes seen at 25^oC is only partially suppressed by Pros25^SuDTS/+ and the survivors are slow developing and sick.

Pros26¹ Prosβ2¹ double heterozygotes show early larval lethality at 25^oC, which is partially suppressed by Pros25^SuDTS/+.

Pros26¹/+ can weakly suppress the lethality of Pros25^SuDTS homozygotes at 25^oC.

(Neuburger et al., 2006)

The multi-socket phenotype found in Pros26¹ mutants is completely suppressed by expression of numb^{ΔC.Scer\UAS.T:Hsap\MYC} (under the control of Scer\GAL4^sca-537.4) with the resultant flies exhibiting only the numb phenotype, namely, balding of the notum. Underneath the balding patches, there are clusters of as many as four neurons.

(Tang et al., 2005)

The constitutive extra vein phenotype in the wing caused by the P{UAS-vn.S}1.1 insertion in the absence of an Scer\GAL4 driver is enhanced in combination with Pros26¹ and following a shift to the restrictive temperature.

(Donaldson et al., 2004)

The prepupal lethality and photoreceptor axon targeting phenotype of not¹/Df(3L)W4 animals is enhanced by one copy of Pros26¹; 78.5% of animals show prepupal lethality and 69.4% show a strong "nonstop" phenotype (more than 50% of photoreceptor cell axon bundles pass the lamina and hyperinnervate the medulla).

(Poeck et al., 2001)

The lqf^bE25 faf^BX3/faf^FO8 mutant phenotype is suppressed by Pros26¹.

(Cadavid et al., 2000)

Double heterozygotes of H^E31 with Pros26¹ or Prosβ2¹ show strong synergistic enhancement of the double socket bristle and microchaete phenotypes. The mild double socket phenotype of N^icd.hs is enhanced by Pros26¹ and Prosβ2¹. The N^icd.hs gene product is more stable in Pros26¹ expressing cells.

(Schweisguth, 1999)

The Pros26¹ dominant temperature sensitive lethal effect is dominantly suppressed by Su(DTS)-1¹. Pros26¹ and Prosβ2¹ are lethal in transheterozygous condition, even at normally permissive temperatures. The synthetic lethal period occurs during early larval development, in contrast to the dominant temperature sensitive lethal period of either allele which occurs during the late pupal stage. This synthetic lethal effect is partially suppressed by Su(DTS)-1¹; a small fraction of + Pros26¹ Su(DTS)-1¹/Prosβ2¹ + + animals survive to adulthood at 18^oC. Does not show a dominant synthetic lethal interaction with Prosβ2^rv19X, Prosβ2^rv3γ, Prosβ2^rv11γ, Prosβ2^rv12γ or Prosβ2^rv3D.

(Smyth and Belote, 1999)

Heterozygosity for Pros26¹ dramatically reduces the severity of the eye phenotype of faf^FO8 homozygotes.

(Huang et al., 1995)

Xenogenetic Interactions

Statement

Reference

Prosβ6¹ does not suppress the protective role of expression of Hsap\ATXN3^{Scer\UAS.WT.T:Zzzz\His6} or DnaJ-1^Scer\UAS.cKa in suppression of eye degeneration in Scer\GAL4^GMR.long>Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} flies. Prosβ6¹ does not alter eye degeneration in Scer\GAL4^GMR.long>Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} flies.

(Tsou et al., 2015)

Reducing proteasome function by introduction of Prosβ6¹ to Scer\GAL4^Bx-MS1096/Mmus\Cul1^Scer\UAS.cHa flies enhances the wing size phenotype.

(Heriche et al., 2003)

The rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is not modified if the flies are also carrying Pros26¹.

(Shulman and Feany, 2003)

Complementation and Rescue Data

Rescued by

Prosβ6¹ is rescued by Prosβ6^+t3.5

(Saville and Belote, 1993)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

6182

Prosβ6¹ pb¹ p^p/TM3, Sb¹ Ser¹

Kyoto

108671

Prosβ6¹ pb¹ p^p/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

Suzuki.

External Crossreferences and Linkouts ( 2 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

U00790

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAC46465

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

DTS-5

(Holden and Suzuki, 1973)

DTS5

(Tsou et al., 2015, Dasgupta et al., 2009, Dasgupta et al., 2009, Warrick et al., 2005, Donaldson et al., 2004, Heriche et al., 2003, Schweisguth, 1999)

Pros26¹

(Rumpf et al., 2014, Seigle et al., 2008, Neuburger et al., 2006)

Pros¹

(Shulman and Feany, 2003)

Prosbeta6¹

(Ribot et al., 2022)

Prosβ6¹

(Wang et al., 2015)

l(3)73Ai¹

(Mandal et al., 2010, Poeck et al., 2001, Cadavid et al., 2000, Smyth and Belote, 1999, Huang et al., 1995, Saville and Belote, 1993)

l(3)DTS-5

(Ashburner et al., 1981)

l(3)DTS5

prosβ6¹

(Hwang et al., 2020, Kim et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (29)

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