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General Information
Symbol
Dmel\hyd1
Species
D. melanogaster
Name
FlyBase ID
FBal0010232
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Hemizygous male testis are smaller than those of wild type males, few germ cell develop past primary spermatocytes. Ovaries of homozygous females are also smaller than those of wild type. Examination of the ovaries revealed they retain a large number of eggs, only a few are laid.

Homozygous germ-line clones do not give rise to vitellogenic egg primordia at 29oC.

Imaginal discs of homozygous larvae are morphologically normal but differentiate abnormally.

Homozygous larvae raised at the non-permissive temperature show extensive overgrowth of the wing discs. The discs have convoluted epithelial outgrowths, mainly in the wing pouch region. The number of gap junctions is reduced in these discs.

At permissive temperatures (20oC or lower), approximately half the expected number of homozygotes eclose, the remainder dying as pharate adults. Those that emerge are sterile, the ovaries of mutant females do not mature, while the reproductive system of males show no obvious morphological defects. They also have other slight abnormalities affecting the derivatives of the eye-antenna, wing, and leg discs. At 22oC all homozygotes die as pharate adults, with greatly reduced heads, often with duplicated antennae and palp structures. The wings, halteres and legs are also abnormal. At 25-29oC no metamorphosis occurs. The eye-antenna disc is reduced to a thread of tissue, while the antennal region is either absent or partially duplicated. The wing and haltere discs are larger than normal and develop additional folds. Eye-antennal discs transplanted into wild-type hosts at 25oC show extreme abnormalities.

temperature-sensitive At 20oC about half the expected number of homozygotes eclose, the remainder dying as pharate adults; those that emerge are sterile, ovaries failing to mature, testes appearing morphologically normal; they also exhibit other slight abnormalities such as extra sex-comb teeth and defective wing margins. At 22oC all flies die as pharate adults with greatly reduced heads, often with duplicated antennal and palpal structures. No metamorphosis accompanies development at 25oC; the eye disc is virtually missing, whereas the wing and haltere discs are severely hyperplastic; disc effects autonomous in transplants. Temperature-shift experiments demonstrate a temperature-sensitive period lasting from the beginning of the second instar until the end of pupation. The 24-hr pulses of 27oC during development reveal that the various phenotypic consequences of homozygosity for this allele have different TSP's. hyd1 wing discs used to demonstrate that extra cellular proliferation is insufficient to induce transdetermination (Shearn et al., 1984). Density of gap junctions in hyperplastic imaginal wing discs markedly reduced (Ryerse and Nagel, 1984); in shift-up experiments, reduction in gap junctions precedes the onset of tissue hyperplasia (Ryerse and Nagel, 1985). Cell death also observed in wing discs as early as twelve hours following application of restrictive temperature (Sedlak, 1986). Mitosis normal (Gatti and Baker, 1989).

External Data
Interactions
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Phenotypic Class
Phenotype Manifest In
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Xenogenetic Interactions
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Reference
Complementation and Rescue Data
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Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Shearn.

Comments
Comments

Temperature-shift experiments show a temperature-sensitive period extending from the beginning of the second instar to the end of pupation. Pulses of 27oC during development have been used to determine more accurately the different temperature-sensitive periods for lethality and for the morphological defects produced by hyd.

Germ-line clonal analysis indicates that the hyd product is required in the germ-line.

Injection of mutant ovaries into ovoD1/+ larvae fails rescue the fertility defect of the transplanted ovaries. Also hemizygous mutant pole cells transplanted into ovoD1/+ larvae are very similar in appearance to ovaries dissected from hemizygous mutant flies. This demonstrates that the requirement for hyd is ovary autonomous.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
hyd1
l(3)C43hs
Name Synonyms
Secondary FlyBase IDs
    References (6)