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General Information
Symbol
Dmel\CtBP87De-10
Species
D. melanogaster
Name
FlyBase ID
FBal0010480
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dCtBP87De-10, CtBPl(3)87De-10
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C13021037T

Reported nucleotide change:

C?T

Amino acid change:

Q229term | CtBP-PA; Q229term | CtBP-PB; Q229term | CtBP-PC; Q229term | CtBP-PD; Q229term | CtBP-PE; Q229term | CtBP-PF; Q229term | CtBP-PG; Q229term | CtBP-PH; Q229term | CtBP-PI; Q229term | CtBP-PJ

Reported amino acid change:

Q229term

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nucleotide substitution: C?T.

Amino acid replacement: Q229term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

CtBP87De-10 clones in wing imaginal discs do not cause apoptosis compared to controls.

CtBP87De-10/+ adults do not exhibit ectopic wings compared to controls.

CtBP87De-10/+ adult flies have one trichogen (hair) and one tormogen (socket) cell per notal microchaeta, similar to wild-type controls.

CtBP87De-10 heterozygotes do not exhibit any size alterations or roughness in eyes when compared to control adults.

CtBP87De-10/CtBP03463 exhibit following eye defects: large and mildly rough eyes with a decrease in the number of interommatidial bristles, and a significant increase in the number of ommatidia; and following head defects: occasional duplicated vertical and orbital bristles, occasional double and/or misoriented orbital and postvertical bristles, and variable loss of interocellar and frontal bristles, large ocelli and surrounding ocellar cuticle while similar distance across the dorsal head between eyes when compared to control pharate adults.

CtBP87De-10/Df(3R)ry615 exhibit a significant increase in the number of ommatidia and following defects in antenna: second and third antennal segments are somewhat larger, the aristae are slightly shorter with the main shaft tapering more prominently along the proximal-distal axis and branches from the main shaft are curved, and the number of bristles on second segment is reduced while Johnston's organs appear normal when compared to control pharate adults.

Almost all mosaic animals with CtBP87De-10 homozygous clones and many mosaic animals with clones (in Minute background), both comprising most of the eye-antennal disc derived structures in the adult head, survive to adulthood as in controls.

Mosaic animals with CtBP87De-10 clones (in Minute background) exhibit increased eye size, minor antennal defects and following head defects: loss of most post-vertical, ocellar, frontal, postorbital, and all interocellar bristles; one or more vertical bristles with extra or double bristles; occasionally missing or duplicated orbital bristles; large ocelli and surrounding ocellar cuticle while they exhibit similar distance across the dorsal head between eyes and no significant change in the number of ommatidia

compared to control adults.

Mosaic animals with CtBP87De-10 clones exhibit larger pre-morphogenetic-furrow clones with significantly more cell proliferation (PH3 staining); and animals with clones (in Minute background) exhibit gaps in the developing ommatidial array when compared to control clones in the third instar larval eye-antennal discs.

CtBP03463/CtBP87De-10 heterozygous pharates display extra dorsal thoracic mechanosensory bristles (additional bristles attached to sockets) and, less frequently, double bristles (two bristles housed in the same socket structure) and bald cuticle (complete loss of both bristles and their associated sockets). Only a subset of bristles are affected.

Within CtBP87De-10 dorsal thorax clones, extra bristles, double bristles forming from the same socket, bald cuticle, and sockets lacking bristles are observed. All but the presutural bristle appear to be affected. The double bristle phenotypes appear exclusively at the anterior scutellar bristle and posterior dorsocentral bristle positions. Bald cuticle appears in all regions except the posterior postalar bristle and presutural regions. Socket without bristle phenotype is observed for all positions except the presutural position.

A putative cell polarity abnormality of the microchaetes is observed in CtBP87De-10 dorsal thorax clones.

CtBP87De-10 mutant cyst progenitor cell clones cannot be recovered.

About 65% of CtBP87De-10, CtBPUbi.T:Hsap\MYC flies show an extra macrochaete phenotype on the dorsal thorax.

Heterozygotes occasionally have bristle with a double-socket phenotype. CtBP03463/CtBP87De-10 pharate adults show bristle shaft defects and shaft loss.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Statement
Reference

CtBP[+]/CtBP87De-10 is an enhancer of visible phenotype of upd11

CtBP[+]/CtBP87De-10 is an enhancer of visible phenotype of Su(H)RC-9Xm

CtBP[+]/CtBP87De-10 is an enhancer of visible | dominant phenotype of HE31

NOT Enhancer of
Statement
Reference

CtBP[+]/CtBP87De-10 is a non-enhancer of visible | adult stage phenotype of Scer\GAL4rn-GAL4-5, hepCA.UAS

CtBP[+]/CtBP87De-10 is a non-enhancer of visible phenotype of upd1GMR.PB

CtBP[+]/CtBP87De-10 is a non-enhancer of visible phenotype of H21

CtBP[+]/CtBP87De-10 is a non-enhancer of visible phenotype of H21, groE73/gro[+]

Suppressor of
Statement
Reference

CtBP[+]/CtBP87De-10 is a suppressor | partially of visible | heat sensitive phenotype of Nl1N-ts1

NOT Suppressor of
Statement
Reference

CtBP[+]/CtBP87De-10 is a non-suppressor of visible | adult stage phenotype of Scer\GAL4rn-GAL4-5, hepCA.UAS

CtBP[+]/CtBP87De-10 is a non-suppressor of visible | adult stage phenotype of Scer\GAL4ptc.PU, wgUAS.cHa.Tag:HA

CtBP[+]/CtBP87De-10 is a non-suppressor of visible phenotype of upd1GMR.PB

CtBP[+]/CtBP87De-10 is a non-suppressor of visible phenotype of H21

CtBP[+]/CtBP87De-10 is a non-suppressor of visible phenotype of H21, groE73/gro[+]

Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Statement
Reference

CtBP[+]/CtBP87De-10 is an enhancer of eye phenotype of upd11

CtBP[+]/CtBP87De-10 is an enhancer of macrochaeta phenotype of Su(H)RC-9Xm

CtBP[+]/CtBP87De-10 is an enhancer of anterior orbital bristle phenotype of HE31

CtBP[+]/CtBP87De-10 is an enhancer of macrochaeta phenotype of HE31

CtBP[+]/CtBP87De-10 is an enhancer of eye phenotype of Scer\GAL4hs.2sev, ttk69.UAS.Tag:HA

NOT Enhancer of
Statement
Reference

CtBP[+]/CtBP87De-10 is a non-enhancer of wing | ectopic phenotype of Scer\GAL4rn-GAL4-5, hepCA.UAS

CtBP[+]/CtBP87De-10 is a non-enhancer of wing | ectopic phenotype of Scer\GAL4ptc.PU, wgUAS.cHa.Tag:HA

CtBP[+]/CtBP87De-10 is a non-enhancer of eye phenotype of upd1GMR.PB

CtBP[+]/CtBP87De-10 is a non-enhancer of macrochaeta phenotype of H21

CtBP[+]/CtBP87De-10 is a non-enhancer of macrochaeta phenotype of H21, groE73/gro[+]

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

CtBP[+]/CtBP87De-10 is a non-suppressor of wing | ectopic phenotype of Scer\GAL4rn-GAL4-5, hepCA.UAS

CtBP[+]/CtBP87De-10 is a non-suppressor of wing | ectopic phenotype of Scer\GAL4ptc.PU, wgUAS.cHa.Tag:HA

CtBP[+]/CtBP87De-10 is a non-suppressor of eye phenotype of upd1GMR.PB

CtBP[+]/CtBP87De-10 is a non-suppressor of macrochaeta phenotype of H21

CtBP[+]/CtBP87De-10 is a non-suppressor of macrochaeta phenotype of H21, groE73/gro[+]

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

In CtBP87De-10/+ background expression of egrUAS.cUa under the control of Scer\GAL4rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) leads to either no ectopic wings (in majority) or ectopic wings (24-hour-expression yielding a lower frequency than 40-hour-expression) on one or two sides compared to controls. Ectopic wings occur most frequently when expression is induced on early stages of third instar larvae. After expression has ceased, wing discs exhibit morphological changes associated with the outgrowth of an ectopic wing pouch from the posterior edge of the notum.

Expression of rprUAS.cUa, wgUAS.cHa.Tag:HA, grndintra.UAS, upd1UAS.cUa or hepUAS.cUa under the control of Scer\GAL4rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) in CtBP87De-10/+ background does not lead to ectopic wings (or ectopic wing pouches in wing discs with upd1UAS.cUa) compared to controls.

Restricted expression of wgUAS.cHa.Tag:HA to second but not third instar larvae (under the control of Scer\GAL4ptc.PU and tub-Gal80[ts]) leads to ectopic wing pouches in wing discs in CtBP87De-10/+ background compared to controls.

40-hour- (but not 24-hour-) expression of hepCA.UAS under the control of Scer\GAL4rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) in CtBP87De-10/+ background leads to occasional ectopic wings compared to controls.

Mosaic animals with CtBP87De-10 danems3 double homozygous clones exhibit significantly fewer ommatidia than single homozygous mosaic eyes and control clones.

Mosaic animals with CtBP87De-10 danems3 danrex35 triple homozygous clones (in Minute background) exhibit defects in ommatidia with a higher frequency when compared to danems3 danrex35 double homozygous clones (in Minute background).

CtBP87De-10 combined with h41, ttkrM730 or Df(3R)awd-KRB leads to an ectopic margin bristle phenotype.

CtBP87De-10 dominantly enhances the bristle defects seen in HE31/+ flies. This enhancement is almost entirely due to shaft-socket transformations, with only a mild effect on bristle loss.

Some of the eye phenotypes caused by expression of ttk69.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4hs.2sev are enhanced by one copy of CtBP87De-10.

Xenogenetic Interactions
Statement
Reference

Atha\ANUbi.T:Hsap\MYC fails to rescue CtBP87De-10 zygotic lethality.

Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Gelbart.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (12)
References (26)