A Database of Drosophila Genes & Genomes

FB2008_06, released July 3, 2008
 

Allele Dmel\l(3)DTS31

General Information
SymbolDmel\l(3)DTS31SpeciesD. melanogaster
NameFlyBase IDFBal0010842
Feature typealleleCreated / Updated2006-08-22/2006-08-22
Associated geneDmel\l(3)DTS3
Allele class
Mutagenethyl methanesulfonate
hide Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Holden and Suzuki, 1973)
Mapped Features and Mutations
Type
Symbol & Location
Additional Notes
References
 
 
 
 
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Progenitor genotype
      Nature of the lesion
      Statement
      Reference
       
       
      Assay mode
      Cytology
      hide Phenotypic Data
      hide Phenotypic Class
      lethal | dominant | conditional ts
      (Walker et al., 1987)
      lethal | dominant | larval stage | conditional ts
      (Holden and Suzuki, 1973)
      lethal | dominant | pupal stage | conditional ts
       
      long lived | dominant | female limited | temperature conditional
      (Simon et al., 2003)
      hide Phenotype Manifest In
      ring gland | conditional ts
       
      prothoracic gland | conditional ts
       
      larval salivary gland & embryo & nuclear chromosome | conditional ts
       
      hide Detailed Description
      Statement
      Reference
      Lethal phase is during the third larval instar stage at 29oC. Heterozygous larvae raised at 29oC are arrested at the third larval instar stage for up to two weeks. They have grossly hypertrophied ring glands due to the growth of the prothoracic gland cells. The nuclei possess polytene chromosomes which are as large as those of salivary gland nuclei; their banding pattern is similar but not identical to that of salivary gland polytene chromosomes, but the puffing patterns are quite different. Salivary gland chromosomes also hypertrophy and lack the normal ecdysone-induced puffs.
       
      Heterozygotes die as third instar larvae at 29oC. The third instar larval stage may be prolonged for 14 days before death occurs. The temperature-sensitive period is during the second to third instar stage.
      (Holden and Suzuki, 1973)
      After 5 days at the restrictive temperature (29.5oC), heterozygous females have an ecdysteroid titre approximately half that of wild-type flies. Egg hatchability decreases with the exposure time of the heterozygous mothers to 29.5oC until after 7-8 days no eggs hatched. This sterility is reversible; heterozygous females shifted to 22oC resume production of some hatchable eggs after 2 days. The lethal phase of the progeny of heterozygous females depends on the length of time the females are kept at the sublethal temperature of 27oC, indicating that the mutation shows a maternal effect. Yolk polypeptide mRNA and protein levels are not reduced in heterozygous females kept at the restrictive temperature.
      (Walker et al., 1987)
      Mutant larvae develop relatively normally at the restrictive temperature of 29oC, but are unable to pupariate.
      (Awad and Truman, 1997)
      Heterozygous female adults show a temperature dependent increase in longevity. At 29oC they show an increase of mean life-span of 42%. Males do not show a significant increase in life span at any of the temperatures tested. Heterozygous females show an increase in resistance to dry starvation; flies exposed to 29oC show an increase in mean survival time at 25oC of about 33% compared to controls. Males show no changes in resistance compared to controls. The increase in longevity seen in heterozygous females at 29oC is decreased in a dose-sensitive manner by feeding the flies 20-OH-ecdysone. The increased survival under dry starvation conditions of heterozygous females is also reversed by feeding the flies 20-OH-ecdysone.
      (Simon et al., 2003)
      hide Interactions
      hide Phenotypic Class
      hide Phenotype Manifest In
      hide Additional Comments
      hide Genetic Interactions
      Statement
      Reference
      hide Xenogenetic Interactions
      Statement
      Reference
      hide Complementation & Rescue Data
      Comments
      hide Stocks ( 3 )
      Bloomington
      3014
      l(3)DTS31/TM2
      Kyoto
      106783
      l(3)DTS31/TM2
      101678
      l(3)DTS31 / TM2, Ubx130 es
      hide Notes on Origin
      Discoverer
      hide Synonyms & Secondary IDs ( 2 )
      Reported As
      Symbol Synonym
      DTS-3
      (Simon et al., 2003, Awad and Truman, 1997, Walker et al., 1987, Holden and Suzuki, 1973)
      l(3)DTS31
       
      Name Synonym
      Secondary FlyBase IDs
        hide References ( 4 )
        Research paper
        Simon et al., 2003, Science 299(5611): 1407--1410
        Steroid control of longevity in Drosophila melanogaster. [FBrf0157215]
        Awad and Truman, 1997, Dev. Biol. 187(2): 283--297
        Postembryonic development of the midline glia in the CNS of Drosophila: proliferation, programmed cell death, and endocrine regulation. [FBrf0094078]
        Walker et al., 1987, J. Insect Physiol. 33: 137--142
        Vitellogenesis and fertility in Drosophila females with low ecdysteroid titres; the l(3)3<up>DTS</up> mutation. [FBrf0046755]
        Holden and Suzuki, 1973, Genetics 73: 445--458
        Temperature-sensitive mutations in Drosophila melanogaster. [FBrf0025064]