FB2021_01, released Feb 18, 2021

Allele: Dmel\14-3-3εj2B10

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General Information
Symbol
Dmel\14-3-3εj2B10
Species
D. melanogaster
Name
FlyBase ID
FBal0010913
Feature type
allele
Associated gene
Dmel\14-3-3ε
Associated Insertion(s)
P{lacW}14-3-3εj2B10
Carried in Construct
Also Known As
l(3)j2B10, D14-3-3εl(3)j2B10
Genomic Maps
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Benton et al., 2002, Chang and Rubin, 1997)
Mutagen
P-element activity
(Chang and Rubin, 1997)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
transposable element insertion site
3R:18,244,702..18,244,709 [+]
(Chen et al., 2003, Su et al., 2001, Spradling et al., 1999, Chang and Rubin, 1997, BDGP Project Members, 1994-1999)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Cytology
      Nature of the lesion
      Statement
      Reference

      Insertion of a P{lacW} element within the first intron.

      (Chang and Rubin, 1997)
      Insertion components
      P{lacW}14-3-3εj2B10
      Product class / Tool use(s)
      Encoded product / tool
      lacZ
      Tagged with
      Tag:NLS(P)
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class

      abnormal body size (with 14-3-3εex4)

      (Nielsen et al., 2008)

      abnormal body size | recessive

      (Nielsen et al., 2008)

      abnormal DNA repair

      (Brodsky et al., 2000)

      abnormal mitotic cell cycle | recessive

      (Su et al., 2001)

      abnormal neuroanatomy

      (Yang and Terman, 2012)

      abnormal neuroanatomy (with 14-3-3εNP1301)

      (Yang and Terman, 2012)

      female fertile (with 14-3-3εex5)

      (Tsigkari et al., 2012)

      female sterile

      (Tsigkari et al., 2012)

      female sterile (with 14-3-3εex4)

      (Tsigkari et al., 2012)

      female sterile (with Df(3R)14-3-3εex24)

      (Tsigkari et al., 2012)

      female sterile (with Df(3R)P14)

      (Benton et al., 2002)

      female sterile | recessive

      (Benton et al., 2002)

      lethal | embryonic stage | maternal effect

      (Perrimon et al., 1996)

      lethal | larval stage | recessive

      (Perrimon et al., 1996)

      lethal | recessive

      (Spradling et al., 1999)

      long lived | dominant

      (Nielsen et al., 2008)

      male fertile (with 14-3-3εex5)

      (Tsigkari et al., 2012)

      male sterile

      (Tsigkari et al., 2012)

      male sterile (with 14-3-3εex4)

      (Tsigkari et al., 2012)

      male sterile (with Df(3R)14-3-3εex24)

      (Tsigkari et al., 2012)

      partially lethal (with Df(3R)14-3-3εex24)

      (Acevedo et al., 2007)

      partially lethal - majority die (with 14-3-3εex4)

      (Acevedo et al., 2007)

      partially lethal - majority die | recessive

      (Acevedo et al., 2007)

      radiation sensitive | dominant

      (Nielsen et al., 2008)

      some die during embryonic stage | recessive

      (Acevedo et al., 2007)

      sterile | recessive

      (Chang and Rubin, 1997)

      viable

      (Benton et al., 2002, Chang and Rubin, 1997)

      viable (with 14-3-3εex5)

      (Acevedo et al., 2007)

      viable (with Df(3R)P14)

      (Benton et al., 2002)

      visible (with 14-3-3εex4)

      (Acevedo et al., 2007)

      visible (with 14-3-3εS-696)

      (Chang and Rubin, 1997)

      visible | recessive

      (Acevedo et al., 2007)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous females lay very few eggs.

      Pole cells appears dispersed all over the embryo in homozygous 16-18 hours embryos with the number of pole cells per gonad being reduced compared to wild type at stages 8 and 11-12.

      (Tsigkari et al., 2012)

      Homozygous and 14-3-3εj2B10/14-3-3εNP1301 embryos show highly penetrant axon guidance defects in the ISNb and SNa axons.

      (Yang and Terman, 2012)

      No retinal phenotypes are seen in 14-3-3εj2B10 mutant flies.

      (Branco et al., 2008)

      The eyes of heterozygous 14-3-3εj2B10 flies appear normal.

      Homozygous 14-3-3εj2B10 mutants are smaller than their isogenic siblings, as measured by whole body size, body weight, and wing size.

      Trans-heterozygous 14-3-3εj2B10/14-3-3εex4 mutants are smaller than their isogenic siblings, as measured by body weight.

      Both homo- and heterozygous 14-3-3εj2B10 mutants display increased sensitivity to ultraviolet irradiation induced apoptosis.

      Mean and maximum lifespan of 14-3-3εj2B10 heterozygotes is significantly higher than that of sibling controls.

      (Nielsen et al., 2008)

      Homozygotes show 75% viability. 14-3-3εex4/14-3-3εj2B10 animals show 71% viability.

      Homozygous adults have smaller wings than control flies. 25% of homozygotes have defects in the anterior crossvein, while 75% have defects in the posterior crossvein.

      26.7% of 14-3-3εex4/14-3-3εj2B10 adults have defects in the anterior crossvein, while 82.4% have defects in the posterior crossvein.

      (Acevedo et al., 2007)

      In 14-3-3εj2B10 homozygotes or 14-3-3εj2B10/Df(3R)P14 females most egg chambers lack a differentiated oocyte: both of the nurse cells with four ring canals develop as nurse cells. The same phenotype is caused by 14-3-3εj2B10 germ-line clones, but not 14-3-3εj2B10 somatic clones in the ovarian follicle cells. The microtubule organizing center which is visible in the posterior of the prospective oocyte as early as stage 2 in wild-type, is absent from 14-3-3εj2B10 mutants at this stage. The centrosomes do not undergo the anterior-to-posterior movement and eventually diffuse away as this cell exits meiosis and adopts a nurse cell fate. In 14-3-3εj2B10 mutant egg chambers where oocyte specification does occur, marker analysis at stage 10 shows a partially penetrant oocyte polarization phenotype, although migration of the oocyte nucleus occurs normally. These oocytes also show defects in microtubule organization.

      (Benton et al., 2002)

      Embryos derived from a cross of 14-3-3εj2B10/Df(3R)Cha7 females to 14-3-3εj2B10/Df(3R)Cha7 males progress through the first 13 mitotic cycles and cellularise without obvious defects. Cells enter mitosis 14 prematurely compared to wild type so that the division pattern of mutant embryos in gastrulation is more advanced than in wild-type embryos of similar gastrulation but is similar to wild-type embryos of more advanced gastrulation. The entire schedule of mitosis is advanced without disrupting the relative order of mitosis in different positions within the embryo. The rate of germ-band elongation is indistinguishable from wild type. Mutant embryos do not show a delay of entry into mitosis 14 after irradiation, in contrast to wild-type embryos.

      (Su et al., 2001)

      In the absence of irradiation, 14-3-3εj2B10 animals have a normal external appearance, normal imaginal disc morphology and normal numbers of mitotic cells in the discs. After irradiation, the number of mitotic cells in 14-3-3εj2B10 discs is greater than the number found in irradiated wild-type discs.

      (Brodsky et al., 2000)

      Homozygotes have normal eyes but are sterile. 14-3-3εS-696/14-3-3εj2B10 flies have rough eyes and a low penetrance of missing photoreceptors in the ommatidia.

      (Chang and Rubin, 1997)

      Germline clones produce normal eggs with no cuticle development.

      (Perrimon et al., 1996)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Enhancer of
      Statement
      Reference

      14-3-3εj2B10 is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4elav.PU, PlexAUAS.Tag:HA

      (Yang and Terman, 2012)

      14-3-3εj2B10/14-3-3epsilon[+] is an enhancer of visible phenotype of Scer\GAL4GMR.PF, ykiUAS.Tag:MYC

      (Ren et al., 2010)

      14-3-3εj2B10/14-3-3epsilon[+], Scer\GAL4GMR.PF is an enhancer of visible phenotype of Scer\GAL4GMR.PF, foxoUAS.Tag:FLAG

      (Nielsen et al., 2008)
      Suppressor of
      Statement
      Reference

      14-3-3εj2B10 is a suppressor of visible phenotype of Hsap\HTT128Q.1-336.UAS, Scer\GAL4GMR.PU

      (Kaltenbach et al., 2007)
      Other
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      14-3-3εj2B10 has nurse cell | ectopic | germline clone phenotype, enhanceable by 14-3-3ζP1188

      (Benton et al., 2002)

      14-3-3εj2B10 has oocyte | germline clone phenotype, enhanceable by 14-3-3ζP1188

      (Benton et al., 2002)

      14-3-3εj2B10 has oocyte phenotype, enhanceable by par-1W3/par-1[+]

      (Benton et al., 2002)
      Suppressed by
      Enhancer of
      Statement
      Reference

      14-3-3εj2B10 is an enhancer of embryonic/larval central nervous system phenotype of Scer\GAL4elav.PU, PlexAUAS.Tag:HA

      (Yang and Terman, 2012)

      14-3-3εj2B10/14-3-3epsilon[+] is an enhancer of eye phenotype of Scer\GAL4GMR.PF, ykiUAS.Tag:MYC

      (Ren et al., 2010)

      14-3-3εj2B10/14-3-3epsilon[+], Scer\GAL4GMR.PF is an enhancer of eye phenotype of Scer\GAL4GMR.PF, foxoUAS.Tag:FLAG

      (Nielsen et al., 2008)

      14-3-3εj2B10 is an enhancer of oocyte phenotype of par-1W3

      (Benton et al., 2002)
      Suppressor of
      Statement
      Reference

      14-3-3εj2B10/14-3-3epsilon[+] is a suppressor of retina phenotype of Hsap\HTT128Q.1-336.UAS, Scer\GAL4GMR.PU

      (Branco et al., 2008)

      14-3-3εj2B10/14-3-3epsilon[+] is a suppressor of retina phenotype of Hsap\ATXN182Q.UAS, Scer\GAL4GMR.PU

      (Branco et al., 2008)

      14-3-3εj2B10 is a suppressor of eye phenotype of Hsap\HTT128Q.1-336.UAS, Scer\GAL4GMR.PU

      (Kaltenbach et al., 2007)

      14-3-3εj2B10/14-3-3εj2B10 is a suppressor of eye phenotype of Ras85DV12.sev

      (Chang and Rubin, 1997)
      Other
      Statement
      Reference

      14-3-3εj2B10/14-3-3epsilon[+], Sema1aUAS.cYa has intersegmental nerve branch ISNb of A1-7 phenotype

      (Yang and Terman, 2012)

      14-3-3εj2B10/14-3-3epsilon[+], Sema1aUAS.cYa has segmental nerve branch SNa of A1-7 phenotype

      (Yang and Terman, 2012)

      14-3-3εj2B10, 14-3-3ζP1375/14-3-3ζP1188 has follicle cell phenotype

      (Benton and St. Johnston, 2003)

      14-3-3εj2B10/14-3-3epsilon[+], 14-3-3ζP1375/14-3-3ζP1188 has follicle cell phenotype

      (Benton and St. Johnston, 2003)

      14-3-3εj2B10/14-3-3epsilon[+], 14-3-3ζP1188 has oocyte | germline clone phenotype

      (Benton et al., 2002)

      14-3-3εj2B10, 14-3-3ζP1188 has oocyte | germline clone phenotype

      (Benton et al., 2002)

      Df(3R)P14/14-3-3εj2B10, EgfrE1 has photoreceptor cell R8 phenotype

      (Chen and Chien, 1999)

      14-3-3εj2B10, 14-3-3ζ2.3 has posterior crossvein phenotype

      (Chang and Rubin, 1997)

      14-3-3εj2B10, 14-3-3ζ2.3 has eye phenotype

      (Chang and Rubin, 1997)

      14-3-3εj2B10, 14-3-3ζ2.3 has eye photoreceptor cell phenotype

      (Chang and Rubin, 1997)

      14-3-3εj2B10, 14-3-3ζ2.3 has ommatidium phenotype

      (Chang and Rubin, 1997)

      14-3-3εj2B10, 14-3-3ζX1 has posterior crossvein phenotype

      (Chang and Rubin, 1997)

      14-3-3εj2B10, 14-3-3ζX1 has eye phenotype

      (Chang and Rubin, 1997)

      14-3-3εj2B10, 14-3-3ζX1 has eye photoreceptor cell phenotype

      (Chang and Rubin, 1997)

      14-3-3εj2B10, 14-3-3ζX1 has ommatidium phenotype

      (Chang and Rubin, 1997)

      14-3-3εj2B10, 14-3-3ζX1/14-3-3zeta[+] has ommatidium phenotype

      (Chang and Rubin, 1997)

      14-3-3εj2B10, 14-3-3ζ2.3/14-3-3zeta[+] has ommatidium phenotype

      (Chang and Rubin, 1997)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of one copy of Sema-1aScer\UAS.cYa under the control of Scer\GAL4how-24B in embryos that are also heterozygous for 14-3-3εj2B10 results in axon guidance defects in the ISNb and SNa nerves, resulting in reduced muscle innervation.

      The axon guidance defects in the central nervous system which are caused by expression of plexAScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav.PU are enhanced if the embryos carry 14-3-3εj2B10.

      The ISNb and SNa axon guidance defects seen in 14-3-3εj2B10/14-3-3εNP1301 embryos are significantly suppressed by expression of either Ras64BVal14.Scer\UAS, ifScer\UAS.cUa or mewScer\UAS.cWa under the control of Scer\GAL414-3-3ε-NP1301.

      (Yang and Terman, 2012)

      The eye overgrowth phenotype caused by expression of ykiScer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF is enhanced by 14-3-3εj2B10/+.

      (Ren et al., 2010)

      The dwarf phenotype of homozygous 14-3-3εj2B10 mutants is reverted in a foxo21 mutant genetic background.

      The lifespan of 14-3-3εj2B10/+, foxo21/+ double heterozygous flies is similar to wild-type levels.

      (Nielsen et al., 2008)

      Follicular epithelium morphogenesis is normal in 14-3-3ζP1188/14-3-3ζP1375 ; 14-3-3εj2B10/+ egg chambers up to stage 4, but the follicle cells subsequently lose their regular cuboidal shape.

      (Benton and St. Johnston, 2003)

      The penetrance of the oocyte to nurse cell transformation phenotype seen in 14-3-3εj2B10 germ-line clones (80% n=106) is dominantly enhanced to 100% by 14-3-3ζP1188. In 14-3-3εj2B10 mutant egg chambers that develop an oocyte, the penetrance of oocyte polarization defects in oocytes at stage 10 is dominantly enhanced by par-1W3. Many 14-3-3ζP1188 germ-line clones in 14-3-3εj2B10/+ females have defects in oocyte specification and polarization.

      (Benton et al., 2002)

      R8 photoreceptor cells fail to form in EgfrE1/+ ; 14-3-3εj2B10/Df(3R)P14 eye discs.

      (Chen and Chien, 1999)

      Homozygotes but not heterozygotes suppress the Ras85DV12.sev rough eye phenotype. 14-3-3ζX1/+ 14-3-3εj2B10/14-3-3εj2B10 or 14-3-3ζ2.3/+ 14-3-3εj2B10/14-3-3εj2B10 flies have slightly roughened eyes, a low penetrance of missing photoreceptors and a gap in the posterior crossvein of the wings in more than 50% of cases.

      (Chang and Rubin, 1997)
      Xenogenetic Interactions
      Statement
      Reference

      One copy of 14-3-3εj2B10 suppresses the retinal phenotype seen when Hsap\HTT128Q.1-336.Scer\UAS is expressed under the control of Scer\GAL4GMR.PU.

      One copy of 14-3-3εj2B10 suppresses the retinal phenotype seen when Hsap\ATXN182Q.Scer\UAS is expressed under the control of Scer\GAL4GMR.PU.

      (Branco et al., 2008)
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Complements: repo03702. Complements: l(3)0582205822. Complements: MED17s2956.

      (BDGP Project Members, 1994-1999)

      The rough eye and missing photoreceptor cell phenotype of 14-3-3εS-696/14-3-3εj2B10 flies is reverted by mobilisation of the P-element in 14-3-3εj2B10. The recessive lethality of the 14-3-3εj2B10 chromosome is not associated with the P-element insertion.

      (Chang and Rubin, 1997)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (12)
      Reported As
      Symbol Synonym
      14-3-3-ej2B10
      (Johannes and Preiss, 2002)
      14-3-30εj2b10
      (Nielsen et al., 2008)
      14-3-3εJ2B10
      (Branco et al., 2008)
      14-3-3εLOF
      (Branco et al., 2008)
      14-3-3εj2B10
      (Yang and Terman, 2012, Ren et al., 2010)
      14-3-3εj2b10
      (Nielsen et al., 2008, Brodsky et al., 2000)
      1433εj2b10
      (Nielsen et al., 2008)
      D14-3-3εl(3)j2B10
      (Tsigkari et al., 2012, Acevedo et al., 2007)
      epsj2B10
      (Tsigkari et al., 2012)
      j2B10
      (Kaltenbach et al., 2007)
      l(3)2B10
      (Chen and Chien, 1999)
      l(3)j2B10
      (Su et al., 2001, Spradling et al., 1999, Chang and Rubin, 1997)
      Name Synonyms
      Secondary FlyBase IDs
        References (19)