Homozygous females lay very few eggs.
Pole cells appears dispersed all over the embryo in homozygous 16-18 hours embryos with the number of pole cells per gonad being reduced compared to wild type at stages 8 and 11-12.
No retinal phenotypes are seen in 14-3-3εj2B10 mutant flies.
The eyes of heterozygous 14-3-3εj2B10 flies appear normal.
Homozygous 14-3-3εj2B10 mutants are smaller than their isogenic siblings, as measured by whole body size, body weight, and wing size.
Trans-heterozygous 14-3-3εj2B10/14-3-3εex4 mutants are smaller than their isogenic siblings, as measured by body weight.
Both homo- and heterozygous 14-3-3εj2B10 mutants display increased sensitivity to ultraviolet irradiation induced apoptosis.
Mean and maximum lifespan of 14-3-3εj2B10 heterozygotes is significantly higher than that of sibling controls.
Homozygotes show 75% viability. 14-3-3εex4/14-3-3εj2B10 animals show 71% viability.
Homozygous adults have smaller wings than control flies. 25% of homozygotes have defects in the anterior crossvein, while 75% have defects in the posterior crossvein.
26.7% of 14-3-3εex4/14-3-3εj2B10 adults have defects in the anterior crossvein, while 82.4% have defects in the posterior crossvein.
In 14-3-3εj2B10 homozygotes or 14-3-3εj2B10/Df(3R)P14 females most egg chambers lack a differentiated oocyte: both of the nurse cells with four ring canals develop as nurse cells. The same phenotype is caused by 14-3-3εj2B10 germ-line clones, but not 14-3-3εj2B10 somatic clones in the ovarian follicle cells. The microtubule organizing center which is visible in the posterior of the prospective oocyte as early as stage 2 in wild-type, is absent from 14-3-3εj2B10 mutants at this stage. The centrosomes do not undergo the anterior-to-posterior movement and eventually diffuse away as this cell exits meiosis and adopts a nurse cell fate. In 14-3-3εj2B10 mutant egg chambers where oocyte specification does occur, marker analysis at stage 10 shows a partially penetrant oocyte polarization phenotype, although migration of the oocyte nucleus occurs normally. These oocytes also show defects in microtubule organization.
Embryos derived from a cross of 14-3-3εj2B10/Df(3R)Cha7 females to 14-3-3εj2B10/Df(3R)Cha7 males progress through the first 13 mitotic cycles and cellularise without obvious defects. Cells enter mitosis 14 prematurely compared to wild type so that the division pattern of mutant embryos in gastrulation is more advanced than in wild-type embryos of similar gastrulation but is similar to wild-type embryos of more advanced gastrulation. The entire schedule of mitosis is advanced without disrupting the relative order of mitosis in different positions within the embryo. The rate of germ-band elongation is indistinguishable from wild type. Mutant embryos do not show a delay of entry into mitosis 14 after irradiation, in contrast to wild-type embryos.
In the absence of irradiation, 14-3-3εj2B10 animals have a normal external appearance, normal imaginal disc morphology and normal numbers of mitotic cells in the discs. After irradiation, the number of mitotic cells in 14-3-3εj2B10 discs is greater than the number found in irradiated wild-type discs.
Homozygotes have normal eyes but are sterile. 14-3-3εS-696/14-3-3εj2B10 flies have rough eyes and a low penetrance of missing photoreceptors in the ommatidia.
Germline clones produce normal eggs with no cuticle development.