FB2025_01 , released February 20, 2025
Allele: Dmel\Stat92Ej6C8
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General Information
Symbol
Dmel\Stat92Ej6C8
Species
D. melanogaster
Name
FlyBase ID
FBal0010984
Feature type
allele
Associated gene
Dmel\Stat92E
Associated Insertion(s)
P{lacW}Stat92Ej6C8
Carried in Construct
Also Known As
l(3)J6C8
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
Progenitor genotype
Associated Insertion(s)
P{lacW}Stat92Ej6C8
Cytology
Description
Allele components
Component
Use(s)
Inserted element
P{lacW}
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      24% of Stat92Ej6C8/Df(3R)H-B79 animals die during embryogenesis and the remainder die during the first larval instar.

      (Ekas et al., 2010)

      Marked homozygous somatic cyst progenitor cell clones in the testis are extremely unstable compared to wild-type control clones.

      Somatic cyst progenitor cells (CPCs) and germline stem cells are completely lost and the hub moves to the inside in most testes after 4 days at 29[o]C in Stat92EF/Stat92Ej6C8 males (raised at 22[o]C). 70% of the testes retain some somatic cyst cells. Marker analysis indicates that the CPCs differentiate into daughter somatic cyst cells in the mutant testes at 29[o]C. When the flies are shifted from 29[o]C back to 18[o]C for 3 days, marker analysis suggests that the daughter somatic cyst cells are able to de-differentiate back into CPCs.

      (Singh et al., 2010)

      After shifting to the restrictive temperature of 29[o]C, germline stem cells, spermatogonia and spermatocytes are gradually lost over time in Stat92EF/Stat92Ej6C8 testes. After 4 days at 29[o]C, germline stem cells are completely lost and only a fraction of testes retain spermatogonia. After 7 days at 29[o]C, germline stem cells, spermatogonia and spermatocytes are completely lost in all Stat92EF/Stat92Ej6C8 testes.

      (Singh et al., 2006)

      Stat92Ej6C8 homozygous female germ line clones develop normally. Early oogenesis is unaffected when follicle stem cells are homozygous for Stat92Ej6C8 (as somatic clones) but cysts containing mutant follicle cells frerquently do not become encapsulated. In contarst, when escort cells are homozygous for Stat92Ej6C8 (as somatic clones), germaria become distorted close to the mutant cells, lack normal patterns of developing cysts, and are suurounded by an expanded muscle sheath and associated basement membrane. Numbers of germline stem cells are reduced in these germaria and mislocalised spectrosomes.

      (Decotto and Spradling, 2005)

      When homozygous mutant eyes are made using somatic clones, the resultant eyes are mostly normal showing a slight reduction in size, some misaligned ommatidia and infrequently, missing antennal structures.

      (Read et al., 2004)

      Follicle cell clones of Stat92Ej6C8 cause a cell-autonomous loss-of-stalk fate. When such Stat92Ej6C8 clones prevent the formation of the anterior stalk of an cyst, this chamber fuses with the adjacent anterior cyst. The oocyte of the anterior egg chamber is then mispositioned. In contrast, when all of the epithelial follicle cells and the polar cells are Stat92Ej6C8 clones, but the stalk cells are wild-type, the oocyte is positioned correctly.

      (Torres et al., 2003)

      Females with homozygous clones in the follicle cells show egg chamber fusions.

      (McGregor et al., 2002)

      Homozygous germ line stem cells are frequently detected in the testis two days after clone induction. However, the percentage of testes containing germ line stem cell clones decreases over time and they are absent by 5 days after clone induction.

      (Tulina and Matunis, 2001)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      Stat92Ej6C8 has abnormal size | adult stage | somatic clone phenotype, enhanceable by Cskj1D8

      (Read et al., 2004)
      Enhancer of
      Statement
      Reference

      Stat92Ej6C8 is an enhancer of abnormal size | adult stage | somatic clone phenotype of Cskj1D8

      (Read et al., 2004)
      NOT Suppressor of
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Stat92EF/Stat92Ej6C8 has cyst progenitor cell | male | heat sensitive phenotype, enhanceable by Scer\GAL4C587/Socs36EUASp.cCa

      (Singh et al., 2010)

      Stat92EF/Stat92Ej6C8 has cyst cell of testis | heat sensitive phenotype, enhanceable by Scer\GAL4C587/Socs36EUASp.cCa

      (Singh et al., 2010)

      Stat92EF/Stat92Ej6C8 has male germline stem cell | heat sensitive phenotype, enhanceable by Scer\GAL4C587/Socs36EUASp.cCa

      (Singh et al., 2010)

      Stat92Ej6C8 has eye | somatic clone phenotype, enhanceable by Cskj1D8

      (Read et al., 2004)
      Suppressed by
      Statement
      Reference

      Stat92EF/Stat92Ej6C8 has spermatogonium | heat sensitive phenotype, suppressible | partially by Scer\GAL4nanos.PG/BHDUAS.cSa

      (Singh et al., 2006)

      Stat92EF/Stat92Ej6C8 has male germline stem cell | heat sensitive phenotype, suppressible | partially by Scer\GAL4nanos.PG/BHDUAS.cSa

      (Singh et al., 2006)

      Stat92EF/Stat92Ej6C8 has spermatogonium | heat sensitive phenotype, suppressible | partially by Scer\GAL4nanos.PG/dppUAS.cUa

      (Singh et al., 2006)

      Stat92EF/Stat92Ej6C8 has male germline stem cell | heat sensitive phenotype, suppressible | partially by Scer\GAL4nanos.PG/dppUAS.cUa

      (Singh et al., 2006)

      Cskj1D8, Stat92Ej6C8 has eye | somatic clone phenotype, suppressible by Btkk00206

      (Read et al., 2004)
      NOT suppressed by
      Statement
      Reference

      Stat92EF/Stat92Ej6C8 has spermatogonium | heat sensitive phenotype, non-suppressible by Scer\GAL4nanos.PG/upd1UAS.cCa

      (Singh et al., 2006)

      Stat92EF/Stat92Ej6C8 has male germline stem cell | heat sensitive phenotype, non-suppressible by Scer\GAL4nanos.PG/upd1UAS.cCa

      (Singh et al., 2006)
      Enhancer of
      Statement
      Reference

      Stat92Ej6C8 is an enhancer of eye | somatic clone phenotype of Cskj1D8

      (Read et al., 2004)
      NOT Enhancer of
      Statement
      Reference

      Stat92Ej6C8, Scer\GAL430A is a non-enhancer of wing phenotype of Pka-C1UAS.cKa, Scer\GAL430A

      (Kiger et al., 2001)
      Suppressor of
      Statement
      Reference

      Stat92Ej6C8 is a suppressor of eye phenotype of upd1GMR.PB

      (Read et al., 2004)
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Stat92EF/Stat92Ej6C8 males expressing Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4C587 show complete loss of somatic cyst progenitor cells, somatic cyst cells, germline stem cells, spermatogonia and spermatocytes after 2 days at 29[o]C.

      (Singh et al., 2010)

      Hyperplasia in adult Malpighian tubule clones expressing Ras85DV12.Scer\UAS (using the MARCM system, under the control of Scer\GAL80αTub84B.PL and Scer\GAL4Scer\FRT.Act5C) is not suppressed by making them also mutant for Stat92Ej6C8.

      (Zeng et al., 2010)

      Expression of BHDScer\UAS.cSa under the control of Scer\GAL4nos.PG significantly slows down the loss of germ cell phenotype that is seen in Stat92EF/Stat92Ej6C8 testes at 29[o]C. After 4 days at 29[o]C, 26% of the double mutant testes contain one or more germline stem cells and most of the testes retain spermatogonia. After 7 days at 29[o]C, 8% of the double mutant testes have one or more germline stem cells and 64% retain spermatogonia.

      Expression of upd1Scer\UAS.cCa under the control of Scer\GAL4nos.PG does not suppress the loss of germ cell phenotype that is seen in Stat92EF/Stat92Ej6C8 testes at 29[o]C.

      Expression of dppScer\UAS.cUa under the control of Scer\GAL4nos.PG significantly slows down the loss of germ cell phenotype that is seen in Stat92EF/Stat92Ej6C8 testes at 29[o]C. After 4 days at 29[o]C, 41% of the double mutant testes contain one or more germline stem cells and all of the testes retain spermatogonia. After 7 days at 29[o]C, 17% of the double mutant testes have one or more germline stem cells and 88% retain spermatogonia.

      (Singh et al., 2006)

      Heterozygosity for Stat92Ej6C8 fails to suppress the large eye phenotype of both Scer\GAL4ey.PH > SerScer\UAS.cSa and Scer\GAL4ey.PH > DlScer\UAS.cHa flies.

      (Reynolds-Kenneally and Mlodzik, 2005)

      The enlarged eye phenotype seen in flies carrying one copy of upd1GMR.PB is strongly suppressed by one copy of Stat92Ej6C8.

      (Bach et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      Stat92E06346

      (BDGP Project Members, 1994-1999)
      Partially rescued by

      Stat92EF/Stat92Ej6C8 is partially rescued by Scer\GAL4C587/Stat92EUAS.cSa

      (Singh et al., 2010)
      Comments

      Expression of Stat92EScer\UAS.cSa under the control of Scer\GAL4C587 rescues the loss of somatic cyst progenitor cells that is seen after 4 days at 29[o]C in the testes of Stat92EF/Stat92Ej6C8 males (raised at 22[o]C). The rescued animals also have wild-type somatic cyst cells and most germline stem cells are also restored.

      (Singh et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      L. and Y. Jan.

      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (7)
      Reported As
      Symbol Synonym
      STAT92Ej6c8
      (Li et al., 2015)
      Stat92Ej6C8
      (Ekas et al., 2010, Zeng et al., 2010, Singh et al., 2007, Decotto and Spradling, 2005)
      l(3)J6C8
      (Tulina and Matunis, 2001)
      l(3)j6C8
      (Spradling et al., 1999)
      l(3)j6C8j6C8
      stat92E6C8
      (Singh et al., 2006)
      stat92Ej6C8
      (Hatini et al., 2013, Singh et al., 2010, Bach et al., 2003)
      Name Synonyms
      Secondary FlyBase IDs
        References (19)