Allele Dmel\Eip74EF^neo24

General Information
Symbol	Dmel\Eip74EF^neo24	Species	D. melanogaster
Name		FlyBase ID	FBal0011039
Feature type	allele	Associated gene	Dmel\Eip74EF
Also Known As	E74^P[neo], E74A^P[neo], E74A[P^neo], E74A^Pneo

Map ( GBrowse )	Untitled Document
Allele class	amorphic allele - genetic evidence, loss of function allele
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	amorphic allele - genetic evidence (Fletcher et al., 1995, Urness and Thummel, 1995) loss of function allele (Fletcher and Thummel, 1995, Fletcher and Thummel, 1995)
Mutagen	P-element activity (Cooley et al., 1988, Fletcher et al., 1995)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3L:17,612,383..17,612,383 (Fletcher and Thummel, 1995, Spradling et al., 1999, Lee et al., 2002, Fletcher et al., 1995, Jiang et al., 2000, Cooley et al., 1988)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{hsneo} insertion in the E74A promoter. (Fletcher and Thummel, 1995) P{hsneo} insertion at position +10 relative to the transcription start site, within the minimal sequences necessary to direct E74A transcription. (Fletcher et al., 1995)
Caused by insertion	P{hsneo}Eip74EF^neo24 (Fletcher and Thummel, 1995, Spradling et al., 1999, Lee et al., 2002, Fletcher et al., 1995, Jiang et al., 2000, Cooley et al., 1988)
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell death (Lee and Baehrecke, 2001) lethal \| pharate adult stage (Fletcher et al., 1995) lethal \| P-stage (Wang et al., 2008) lethal \| recessive (Fletcher and Thummel, 1995, Spradling et al., 1999, Cooley et al., 1988)
Phenotype Manifest In
	adult epidermis (Fletcher and Thummel, 1995) embryonic/larval midgut \| prepupal stage (with Df(3L)81k19) (Lee et al., 2002) embryonic/larval salivary gland (Wang et al., 2008) embryonic/larval salivary gland \| embryonic stage (Jiang et al., 2000) salivary gland (Lee and Baehrecke, 2001)
Detailed Description
	Statement Reference Mutants show defects in destruction of the larval salivary glands, having persistant larval salivary glands at the pupal stage. (Wang et al., 2008) At the onset of pupation, as staged by head eversion, Eip74EF^neo24/Df(3L)81k19 mutant animals exhibit the following phenotypes: 1. Mutant larval midguts are extremely condensed and the adult and larval epithelia have separated such that a defined adult lumen exists. These mutants also form an adult midgut epithelium, and the larval proventriculus and gastric caeca are destroyed. Thus, Eip74EF^neo24/Df(3L)81k19 larval midguts appear to be arrested at a stage of destruction that is similar to the midgut of wild-type animals 6-12 h following puparium formation. 2. DNA fragmentation is detected in all larval midgut cells of mutant animals 3. Mutant larval midgut cells appear to die normally and have numerous autophagic structures, including mitochondria in vacuoles, crystalline inclusions, and myelin-like membrane swirls. Between the white prepua stage and head eversion, Eip74EF^neo24/Df(3L)81k19 mutant midguts decrease in length by an average of 81% in length compared to 85% in the wild-type. (Lee et al., 2002) Eip74EF^neo24 mutant pupae exhibit DNA fragmentation in the salivary gland as normal. (Lee et al., 2002) Mutant salivary gland cells lack large eosin positive vacuoles and plasma membranes and show variable chromosome banding - phenotypes associated with autophagy initiating in salivary gland cells but not progressing. Salivary gland cells contain nascent autophagic vacuoles composed of rough endoplasmic reticulum enclosing cytoplasmic components including mitochondria and smaller vacuoles. (Lee and Baehrecke, 2001) Approximately 20% of Eip74EF^neo24/Df(3L)81k19 animals have salivary glands at 24 hours after puparium formation, in contrast to wild-type pupae where they are completely destroyed by 16 hours after puparium formation. (Jiang et al., 2000) Homozygous mutant γ neurons (generated in the brain as somatic clones) exhibit no detectable abnormalities in larval or adult stages. (Lee et al., 2000) Development of hemizygous pupae progresses normally but tanning is delayed by several hours. Some fail to form a gas bubble or undergo pupation. Homozygous and hemizygous pharate adults dissected from the pupal case display leg twitches and abdominal contractions and are inviable. (Fletcher et al., 1995) Head eversion is not affected in mutants. (Fletcher and Thummel, 1995)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Eip74EF^neo24, br^2Bc-2 has lethal \| recessive \| prepupal stage phenotype (Fletcher and Thummel, 1995, Fletcher and Thummel, 1995)
Phenotype Manifest In
NOT Enhancer of
Statement Reference Eip74EF^neo24 is a non-enhancer of phenotype of zip^Ebr (Halsell and Kiehart, 1998)
Other
Statement Reference Eip74EF^neo24, br^2Bc-2 has imaginal disc phenotype (Fletcher and Thummel, 1995, Fletcher and Thummel, 1995) Eip74EF^neo24, br^2Bc-2 has puparium phenotype (Fletcher and Thummel, 1995, Fletcher and Thummel, 1995) Eip74EF^neo24, br⁵ has adult head phenotype (Fletcher and Thummel, 1995) Eip74EF^neo24, br⁵ has leg phenotype (Fletcher and Thummel, 1995, Fletcher and Thummel, 1995) Eip74EF^neo24, br⁵ has wing phenotype (Fletcher and Thummel, 1995, Fletcher and Thummel, 1995) Eip74EF^neo24, br^rbp-5 has head phenotype (Fletcher and Thummel, 1995)
Additional Comments
Genetic Interactions
Statement Reference Double heterozygotes with br^rbp-5, br⁵ or br^2Bc-2 show no effect on development. Heterozygous br^rbp-5 females and mutant br^rbp-5 males homozygous for Eip74EF^neo24 pupariate normally. Half of each group die during prepupal development, resembling severe phenotype Eip74EF^neo24 mutants. 8% display novel phenotypes. Head eversion fails and rudimentary appendages/cryptocephalic head structures develop. 58% of Eip74EF^neo24 mutants carrying a single effective dose of br⁺ arrest during the prepupal period. 20% of these prepupae show a misshapen puparium. A novel microcephalic phenotype results from partial head eversion, though eyes do develop. Leg and wing structures are absent. Lethal phenotype is more severe than either Eip74EF^neo24 or br⁵ alone. More than 25% of Eip74EF^neo24 carrying a single effective dose of br⁺ formed a misshapen puparium. This phenotype is 100% penetrant in a br^2Bc-2 mutant background. Most double mutants die during the prepupal stage, earlier than either br^2Bc-2 or Eip74EF^neo24 mutants alone. In 16% of double mutants eye development proceeds to the point where pigment can be observed, but other discs do not undergo morphogenesis. (Fletcher and Thummel, 1995)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Eip74EF^v4 (Kennison, 1999.2.11)
Comments
Stocks ( 1 )
Bloomington	10262 mwh¹ P{hsneo}Eip74EF^neo24 red¹ e¹/TM3, ry^RK Sb¹ Ser¹
Notes on Origin
Discoverer	A. Spradling. Cooley et al.

Comments
	Late puffs normally active at puparium formation are significantly reduced in size in hemizygous white prepupae, early late and early puffs are relatively unaffected. (Fletcher et al., 1995) Amorphic for the E74A transcription unit. (Fletcher and Thummel, 1995) Complements: frc⁰⁰⁰⁷³. Complements: frc⁰²⁶¹⁹. Complements: l(3)02634⁰²⁶³⁴. Complements: l(3)j11B2^j11B2. (BDGP Project Members, 1994-1999)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	E74^P[neo] (Biyasheva et al., 2001, Lee et al., 2000, Jiang et al., 2000, Halsell and Kiehart, 1998, Urness and Thummel, 1995, Fletcher and Thummel, 1995, Fletcher et al., 1995, Fletcher and Thummel, 1995) E74A[P^neo] (Martin and Baehrecke, 2004, Lee et al., 2002) E74A^neo24 (Ihry et al., 2012) E74A^P[neo] (Lee et al., 2002, Lee and Baehrecke, 2001, Lee et al., 2003) E74A^Pneo (Kraft et al., 2006, Daish et al., 2003) Eip74^neo24 (Wang et al., 2008) Eip74EF^neo24 (Christensen et al., 2008.4.15, Christensen et al., 2008.4.15, Christensen et al., 2008.4.15) l(3)neo24 (Spradling et al., 1999)
Name Synonym
Secondary FlyBase IDs

References ( 24 )

Generate a list of	All references relating to this allele ( 24 )

List References by type	Research paper ( 19 ) Personal communication to FlyBase ( 5 )
Recent research papers ( 1 )
	Ihry et al., 2012, PLoS Genet. 8(11): e1003085 Translational Control by the DEAD Box RNA Helicase belle Regulates Ecdysone-Triggered Transcriptional Cascades. [FBrf0220149] Show all research papers ...

Allele Dmel\Eip74EFneo24

Allele Dmel\Eip74EF^neo24