Allele Dmel\E2f^rM729

General Information
Symbol	Dmel\E2f^rM729	Species	D. melanogaster
Name		FlyBase ID	FBal0011124
Feature type	allele	Associated gene	Dmel\E2f
Also Known As	de2f1^rm729, dE2F1⁷²⁹, E2F^rm729, dE2F⁷²⁹, E2f⁷²⁹

Map ( GBrowse )	Untitled Document
Allele class	loss of function allele
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	loss of function allele (Neufeld et al., 1998)
Mutagen	P-element activity
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3R:17,452,267..17,452,267 (Duronio et al., 1995, BDGP Project Members, 1994-1999, Reis and Edgar, 2004, Neufeld et al., 1998, Sawado et al., 1998, Brook et al., 1996, Steele et al., 2009)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{PZ} is inserted near to the translation start site, such that the Ecol\lacZ coding region is in the same orientation as E2f. (Brook et al., 1996) P{PZ} is inserted 48 nucleotides upstream of the initiator methionine, with the Ecol\lacZ in the same orientation as E2f. (Duronio et al., 1995)
Caused by insertion	P{PZ}E2f^rM729 (Duronio et al., 1995, BDGP Project Members, 1994-1999, Reis and Edgar, 2004, Neufeld et al., 1998, Sawado et al., 1998, Brook et al., 1996, Steele et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	developmental rate defective (with E2f⁹¹) (Frolov et al., 2001) increased cell size \| somatic clone (Neufeld et al., 1998) lethal \| larval stage (with E2f⁹¹) (Frolov et al., 2001) lethal \| recessive (Du, 2000, Duronio et al., 1995)
Phenotype Manifest In
	central nervous system (Duronio et al., 1995) embryonic/larval optic lobe (Du, 2000) eye (Brook et al., 1996) eye-antennal disc (Brook et al., 1996) eye disc (Du, 2000) eye disc & mitotic cell cycle (Du, 2000) eye disc \| somatic clone (Baonza and Freeman, 2005, Ambrus et al., 2007) imaginal disc (with E2f⁹¹) (Frolov et al., 2001) neuroblast (Duronio et al., 1995) ommatidium (Brook et al., 1996) photoreceptor cell (Brook et al., 1996) rhabdomere (Brook et al., 1996) wing disc \| somatic clone (Neufeld et al., 1998)
Detailed Description
	Statement Reference Homozygous clones in the eye disc are very small. (Ambrus et al., 2007) E2f[rM729] eye disc clones in a Minute/+ background are extremely small. (Baonza and Freeman, 2005) E2f⁹¹/E2f^rM729 transheterozygous larvae, are much smaller than wild-type, 5 days after egg laying. They fail to develop into 3rd instar larvae. (Frolov et al., 2001) E2f⁹¹/E2f^rM729 transheterozygotes can develop into the late second instar or early third instar larval stage at a very slow pace (around 13 days after egg laying), but do not develop into pupae. BrdU incorporation is not seen in the optic lobe region of the brain in day 13 E2f⁹¹/E2f^rM729 larvae, in contrast to wild-type third instar larvae. The eye discs of E2f⁹¹/E2f^rM729 larvae are very small and have only a few cells labelled with BrdU with no specific pattern. (Du, 2000) Cells in homozygous clones induced in the wing pouch of the wing disc become enlarged and are gradually lost from the disc epithelium. (Neufeld et al., 1998) Clones induced in the eye in the first or second larval instar are not detected in the adult eye. They can be detected in the eye disc, but are much smaller than the adjacent twin spots, with fewer cells. These cells have initiated neuronal differentiation. Resulting ommatidia have incomplete sets of photoreceptors, and photoreceptors that are present often have abnormal morphology. Rhabdomeres are small in diameter, the rhabdomeres of the outer photoreceptors may project centrally and the photoreceptors do not extend the full length of the retina. (Brook et al., 1996) Cells in the CNS that normally enter endocycles fail at S₁₇. Replication either fails or occurs at a very low rate. (Duronio et al., 1995)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference E2f⁹¹/E2f^rM729 has developmental rate defective phenotype, suppressible by Df(2L)G5.1/E2f2^76Q1 (Frolov et al., 2001) E2f⁹¹/E2f^rM729 has developmental rate defective phenotype, suppressible by Df(2R)vg-B/Dp^a2 (Frolov et al., 2001) E2f⁹¹/E2f^rM729 has lethal phenotype, suppressible by Rbf¹⁴/Rbf^120a (Du, 2000)
Enhancer of
Statement Reference E2f^rM729 is an enhancer of visible \| recessive phenotype of CycE^JP (Secombe et al., 1998) Rbf^120a/E2f^rM729 is an enhancer of increased cell death \| somatic clone phenotype of Tsc1^f01910 (Hsieh et al., 2010)
NOT Enhancer of
Statement Reference E2f^rM729 is a non-enhancer of visible phenotype of Dp^GMR.PD, E2f^GMR.PD (Staehling-Hampton et al., 1999)
Suppressor of
Statement Reference E2f[+]/E2f^rM729 is a suppressor of increased cell death phenotype of Dref^Scer\UAS.cSa, Scer\GAL4^GMR.PS (Hirose et al., 2001) E2fⁱ²/E2f^rM729 is a suppressor \| partially of lethal \| recessive phenotype of Rbf¹⁴ (Du, 2000) E2f^rM729 is a suppressor of increased cell death \| somatic clone phenotype of Rbf^120a, Tsc1^f01910 (Hsieh et al., 2010)
NOT Suppressor of
Statement Reference E2f^rM729 is a non-suppressor of visible phenotype of Dp^GMR.PD, E2f^GMR.PD (Staehling-Hampton et al., 1999)
Other
Statement Reference E2f2^g5/E2f2^76Q1, E2f⁹¹/E2f^rM729 has decreased cell death \| wandering third instar larval stage phenotype (Moon et al., 2005) E2fⁱ²/E2f^rM729, Rbf¹⁴ has viable \| partially phenotype (Du, 2000)
Phenotype Manifest In
Suppressed by
Statement Reference E2f⁹¹/E2f^rM729 has embryonic/larval optic lobe phenotype, suppressible by Rbf¹⁴/Rbf^120a (Du, 2000) E2f⁹¹/E2f^rM729 has eye disc & mitotic cell cycle phenotype, suppressible by Rbf¹⁴/Rbf^120a (Du, 2000) E2f⁹¹/E2f^rM729 has eye disc phenotype, suppressible by Rbf¹⁴/Rbf^120a (Du, 2000) E2f⁹¹/E2f^rM729 has imaginal disc phenotype, suppressible by Df(2L)G5.1/E2f2^76Q1 (Frolov et al., 2001) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by B52^3d16 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by B52^3d24 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by B52^5d21 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by bel^7d19 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Doa^5d7 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Doa^5d19 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Doa^6a21 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Doa^6a52 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Doa^6d2 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by gfzf^6a27 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by l(3)mbt^3d23 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by l(3)mbt^3d33 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by l(3)mbt^4d16 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by l(3)mbt^4d30 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by l(3)mbt^5d6 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by l(3)mbt^5d8 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by l(3)mbt^6a14 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by l(3)mbt^6a31 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Slimp^7d21 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Slimp^f07858 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Su(E1)-A^6a39 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Su(E1)-A^7d13 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Su(E1)-B^4d26 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Su(E1)-B^6a11 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Su(E1)-C^1d24 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Su(E1)-C^3d28 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Su(E1)-D^6a47 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Su(E1)-E^3d25 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Su(E1)-F^6b9 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Su(E1)-G^6a3 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Su(E1)-H^6a50 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Su(E1)-I^6d9 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Su(E1)-J^6a8 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Su(E1)-K^6b12 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Su(E1)-L^6b19 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Su(E1)-M^6b31 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Su(E1)-N^4d22 (Ambrus et al., 2009) E2f^rM729 has eye \| somatic clone phenotype, suppressible \| somatic clone by Su(E1)-O^6a19 (Ambrus et al., 2009) E2f^rM729 has eye disc \| somatic clone phenotype, suppressible \| partially \| somatic clone by bel^7d19 (Ambrus et al., 2007) E2f^rM729 has eye disc \| somatic clone phenotype, suppressible \| partially \| somatic clone by bel^L4740 (Ambrus et al., 2007) E2f^rM729 has eye disc \| somatic clone phenotype, suppressible \| partially \| somatic clone by E2f2^c03344 (Ambrus et al., 2007) E2f^rM729 has eye disc \| somatic clone phenotype, suppressible \| partially \| somatic clone by smo¹ (Ambrus et al., 2007)
NOT suppressed by
Statement Reference E2f^rM729 has eye disc \| somatic clone phenotype, non-suppressible by Scer\GAL4^GMR.PF/Su(H)^{Scer\UAS.T:Hsim\VP16}/Scer\GAL4^GMR.PF (Baonza and Freeman, 2005)
Enhancer of
Statement Reference E2f^rM729, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of E2f2^Scer\UAS.cFa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Frolov et al., 2001) E2f^rM729, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of E2f2^Scer\UAS.cFa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Frolov et al., 2001) E2f^rM729 is an enhancer of eye disc \| somatic clone phenotype of Tsc1^f01910 (Hsieh et al., 2010) E2f^rM729 is an enhancer of eye phenotype of CycE^JP (Secombe et al., 1998) Rbf^120a/E2f^rM729 is an enhancer of eye disc \| somatic clone phenotype of Tsc1^f01910 (Hsieh et al., 2010)
Suppressor of
Statement Reference E2f[+]/E2f^rM729 is a suppressor of eye phenotype of Dref^Scer\UAS.cSa, Scer\GAL4^GMR.PS (Hirose et al., 2001) E2fⁱ²/E2f^rM729 is a suppressor of eye \| somatic clone phenotype of Rbf^15aΔ, rno¹ (Steele et al., 2009) E2fⁱ²/E2f^rM729 is a suppressor of photoreceptor cell R8 \| somatic clone \| third instar larval stage phenotype of Rbf^15aΔ, rno¹ (Steele et al., 2009) E2f^rM729 is a suppressor of eye disc \| somatic clone phenotype of Rbf^120a, Tsc1^f01910 (Hsieh et al., 2010) E2f^rM729 is a suppressor of morphogenetic furrow \| somatic clone phenotype of Rbf^120a, Tsc1^f01910 (Hsieh et al., 2010)
Other
Statement Reference E2f2^g5/E2f2^76Q1, E2f⁹¹/E2f^rM729 has wing disc \| wandering third instar larval stage phenotype (Moon et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference Tsc1[f01910] E2f[rM729] double mutant eye disc clones in a wild-type background are smaller than Tsc1[f01910] single mutant eye disc clones. When Tsc1[f01910] E2f[rM729] double mutant clones are generated in Rbf[120a], the sizes of the Tsc1[f01910] E2f[rM729] double mutant clones are much smaller than that of Tsc1[f01910] single mutant clones. The prevailing cell death phenotype observed in Rbf[120a] Tsc1[f01910] double mutant clones at the morphogenetic furrow is no longer present in Rbf[120a] E2f[rM729] Tsc1[f01910] triple mutant cells, indicating that the increased level of ectopic cell death observed in Rbf[120a] Tsc1[f01910] double mutant cells is E2f-dependent. (Hsieh et al., 2010) Each of l(3)mbt[6a14], l(3)mbt[4d30], l(3)mbt[3d23], l(3)mbt[5d8], l(3)mbt[4d16], l(3)mbt[5d6], l(3)mbt[6a31], l(3)mbt[3d33], Doa[6d2], Doa[6a21], Doa[5d7], gfzf[6a27], B52[5d21], B52[3d16], B52[3d24], CG31133[7d21], CG31133[f07858], bel[7d19], Su(E1)-A[7d13], Su(E1)-A[6a39], Su(E1)-B[4d26], Su(E1)-B[6a11], Su(E1)-C[1d24], Su(E1)-C[3d28], Su(E1)-D[6a47], Su(E1)-E[3d25], Su(E1)-F[6b9], Su(E1)-G[6a3], Su(E1)-H[6a50], Su(E1)-I[6d9], Su(E1)-J[6a8], Su(E1)-K[6b12], Su(E1)-L[6b19], Su(E1)-M[6b31], Su(E1)-N[4d22] and Su(E1)-O[6a19] can suppress the proliferation block of E2f[rM729] clones, allowing recovery of double mutant clones in the eye. The second mitotic wave is strongly restored in l(3)mbt[3d33] E2f[rM729] and Su(E1)-A[7d13] E2f[rM729] double mutant clones in the eye disc. The second mitotic wave is weakly rescued in CG31133[7d21] E2f[rM729] and gfzf[6a27] E2f[rM729] double mutant clones in the eye disc, with the double mutant cells entering the second mitotic wave several columns more posterior than in wild-type cells. In addition BrdU incorporation persists several columns more posterior in the double mutant cells than in the adjacent wild-type cells. The second mitotic wave is weakly rescued in Doa[6d2] E2f[rM729] double mutant clones in the eye disc, with double mutant cells only being sporadically labelled with BrdU in the second mitotic wave. Those double mutant cells that do incorporate BrdU are always found to be significantly more than adjacent wild-type cells that incorporate BrdU. (Ambrus et al., 2009) The shiny-eye and multiple photoreceptor-R8 phenotype seen in rno[1], Rbf[15aΔ] eye somatic mutant clones is significantly suppressed in a E2f[i2]/E2f[rM729] mutant background. (Steele et al., 2009) Relatively large E2f2[c03344] E2f[rM729] double mutant clones can be recovered in the eye disc. The small size of E2f[rM729] clones in the eye disc is partially rescued if they are also mutant for either bel[7d19], bel[L4740] or smo[1]. (Ambrus et al., 2007) Scer\GAL4[GMR.PF]-driven expression of Su(H)[Scer\UAS.T:Hsim\VP16] is unable to rescue the small size of E2f[rM729] eye disc clones. (Baonza and Freeman, 2005) In wild-type animals, exposure to 40Gy of γ rays induces a distinct pattern of apoptosis in the wing disc, focussed on the the wing pouch, but excluding the DV boundary region and prospective vein. In E2f^rM729/E2f⁹¹; E2f2^76Q1/E2f2^g5 animals, the same irradiation treatment induces a different pattern of apoptosis a smaller number of apoptotic cells are clustered in the center of the wing pouch, many in the D-V boundary region or prospective veins. (Moon et al., 2005) The addition of E2f2^76Q1/Df(2L)G5.1 to E2f⁹¹/E2f^rM729 flies almost completely suppresses the E2f⁹¹/E2f^rM729 phenotypes - mutants develop normally without any significant delay in larval growth, reach pupal stage and finally die as mid- or late pupae. The pattern of DNA synthesis in eye discs is largely normal. The addition of Dp^a2/Df(2R)vg-B to E2f⁹¹/E2f^rM729 flies almost completely suppresses the E2f⁹¹/E2f^rM729 phenotypes - mutants develop normally without any significant delay in larval growth. (Frolov et al., 2001) The rough eye phenotype seen upon expression of Dref[Scer\UAS.cSa] in the developing eye under the control of Scer\GAL4[GMR.PS] is suppressed in an E2f[rM729] heterozygous background. (Hirose et al., 2001) Approximately 50% of the expected number of Rbf¹⁴/Y ; E2fⁱ²/E2f^rM729 flies survive to the adult stage, and the development of these flies is not significantly delayed compared to sibling flies. The surviving flies have normal eyes, normal macrochaetae on the notum and normal wings and legs. The larval lethality of E2f⁹¹/E2f^rM729 animals can be suppressed by Rbf^120a/Rbf¹⁴; the larvae develop into large late third instar larvae and many can pupate. Some Rbf^120a/Rbf¹⁴ ; E2f⁹¹/E2f^rM729 animals can develop into pharate adults with adult eyes, legs and wing structures. The development of these larvae is significantly retarded compared to wild-type larvae, with the earliest pupae observed at around day 11 after egg laying. The normal pattern of DNA replication in the optic lobe region is restored in E2f⁹¹/E2f^rM729 larvae that are also carrying Rbf^120a/Rbf¹⁴. In Rbf^120a/Rbf¹⁴ ; E2f⁹¹/E2f^rM729 eye discs the overall pattern of DNA replication is normal. No significant cell death is seen in these eye discs. In late third instar discs there are about 25 rows of developing photoreceptor cells (as in wild-type discs) but there are much fewer cells anterior to the morphogenetic furrow than in wild type. (Du, 2000) Dominantly enhances the rough eye phenotype of CycE^JP homozygotes. (Secombe et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	E2f⁰⁷¹⁷² (BDGP Project Members, 1994-1999)
Comments
Stocks ( 2 )
Bloomington	34054 ry⁵⁰⁶ P{PZ}E2f^rM729/TM3, Sb¹ 35849 w^*; P{neoFRT}82B P{PZ}E2f^rM729/TM3, Sb¹
Notes on Origin
Discoverer	G. Rubin.

Comments
	mir-11[Δ1] complements E2f[rM729]. (Truscott et al., 2011) Transposase-mediated excision of the P{PZ} produces ry^- flies that lack both the insertion and the lethal phenotype. (Duronio et al., 1995) Complements: InR⁰⁵⁵⁴⁵. (BDGP Project Members, 1994-1999)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	dE2F1⁷²⁹ (Reis and Edgar, 2004) de2f1⁷²⁹ (Moon et al., 2005, Ambrus et al., 2009, Ambrus et al., 2007, Hsieh et al., 2010) de2f1^rm729 (Frolov et al., 2003, Frolov et al., 2001, Du, 2000) dE2f1^rM729 (Truscott et al., 2011) dE2f1^rm729 (Steele et al., 2009) dE2F1^rM729 (Baonza and Freeman, 2005) dE2F⁷²⁹ (Sawado et al., 1998, Neufeld et al., 1998, Hirose et al., 2001) dE2F^RM729 (Secombe et al., 1998) E2f⁷²⁹ (Brook et al., 1996, Hirose et al., 2001) E2F^rm729 (Hwang et al., 2002, Staehling-Hampton et al., 1999, Asano and Wharton, 1999) E2f^rM729 E2f^rm729 (Li et al., 2010) l(3)rM729^rM729
Name Synonym
Secondary FlyBase IDs

References ( 23 )

Generate a list of	All references relating to this allele ( 23 )

List References by type	Research paper ( 21 ) Personal communication to FlyBase ( 2 )
Recent research papers ( 1 )
	Truscott et al., 2011, Genes Dev. 25(17): 1820--1834 mir-11 limits the proapoptotic function of its host gene, dE2f1. [FBrf0215211] Show all research papers ...

Allele Dmel\E2frM729

Allele Dmel\E2f^rM729