FlyBase Allele Report: Dmel\Sse[13m]

General Information

Symbol

Dmel\Sse^13m

Species

D. melanogaster

Name

FlyBase ID

FBal0011182

Feature type

allele

Associated gene

Dmel\Sse

Associated Insertion(s)

Carried in Construct

Also Known As

l(3)13m-281

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Contains a deletion of four base pairs between the positions encoding the invariant His and Cys residues resulting in a frame shift and a premature translational stop.

(Jager et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:5,660,412..5,660,415 [-]

Comment:

Approximate location of 4bp deletion in the region of the Leu475 and Gln476 residues that results in a frame shift and a premature translational stop (exact boundaries not reported).

(Jager et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cell cycle

(Gatti and Baker, 1989)

chromosome | larval stage (with Sse^dft)

(Cipressa et al., 2016)

imaginal disc

(Gatti and Baker, 1989)

imaginal disc | larval stage

(Gatti and Baker, 1989)

larval neuroblast | larval stage (with Sse^dft)

(Cipressa et al., 2016)

mitotic cell cycle (with Df(3L)SseA)

(Jager et al., 2001)

Detailed Description

Statement

Reference

Sse^dft/Sse^13m larval brains have telomeric fusions and endoreduplication phenotypes: neuroblasts have few dividing cells (around 3 metaphases per brain) and most cells are endoreduplicated (showing bundles of 2, 4, and 8 sister chromosomes and ploidy levels from 4n to 32n), and a large number of metaphases contain 64 (16n) or more than 64 chromosomes; some endoreduplicated chromosomes are also fused at their ends, giving rise to multicentric chromosome configurations and rings. Telomeric fusion frequency (ratio between number of double telomere attachments and total number of chromosomes, which does not change significantly with the degree of endoreduplication) is increased.

(Cipressa et al., 2016)

In Sse/Df(3L)SseA the brains of early second instar mutant larval brains, the number of cells during early mitotic phases until anaphase are reduced to about 40% of wild-type - prophase and metaphase figures are seen at similar frequencies to wild-type, but anaphase figures are almost completely absent. Second instar larval brains have 95% of the mitotic cells containing either diplo-chromosomes or more extensively endoreplicated chromosomes. In addition to the presence of endoreplicated chromosomes, mitotic figures in mutants frequently contain aneuploid numbers of chromosomes, however polyploid figures with normal chromosomes are never seen. In third instar larvae, mutant brains contain very few cells in the early mitotic phases (5% compared to controls) These cells are abnormally large and have very high levels of DNA. No normal anaphase or telophase figures are seen.

(Jager et al., 2001)

Imaginal discs of homozygous larvae are small. Defects in the cell cycle: few or no dividing cells and cell division failures that result in highly polyploid cells.

(Gatti and Baker, 1989)

imaginal discs small highly polyploid, endoreduplicated cells; very low mitotic index

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Sse^13m/Sse[+] is a suppressor of abnormal mitotic cell cycle | maternal effect | embryonic stage phenotype of CycB^+t10

(Ji et al., 2005)

Sse^13m/Sse[+] is a suppressor of lethal | maternal effect | embryonic stage | cold sensitive phenotype of thr^ΔVQ.Tag:MYC

(Herzig et al., 2002)

Phenotype Manifest In

Enhancer of

Statement

Reference

Sse^13m/Sse[+] is an enhancer of astral microtubule | embryonic cycle 9 | maternal effect phenotype of CycB^+t10

(Ji et al., 2005)

NOT Enhancer of

Statement

Reference

Sse^13m/Sse[+] is a non-enhancer of wing margin phenotype of ct^K

(Dorsett et al., 2005)

Suppressor of

Statement

Reference

Sse^13m/Sse[+] is a suppressor of embryo | maternal effect | embryonic cycle 10 phenotype of CycB^+t10

(Ji et al., 2005)

Sse^13m/Sse[+] is a suppressor of embryo | maternal effect | embryonic cycle 14 phenotype of CycB^+t10

(Ji et al., 2005)

Sse^13m/Sse[+] is a suppressor of microtubule | maternal effect | embryonic cycle 9 phenotype of CycB^+t10

(Ji et al., 2005)

Sse^13m/Sse[+] is a suppressor of embryo | maternal effect | embryonic stage 5 phenotype of thr^ΔVQ.Tag:MYC

(Herzig et al., 2002)

NOT Suppressor of

Statement

Reference

Sse^13m/Sse[+] is a non-suppressor of wing margin phenotype of ct^K

(Dorsett et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Sse^13m heterozygosity does not affect the wing margin nick phenotype of ct^K mutants.

(Dorsett et al., 2005)

The delay in onset of anaphase (measured during cycles 6-11) seen in embryos with two extra copies of the maternal CycB⁺ gene dose (derived from females carrying copies of CycB^+t10) is suppressed by Sse^13m. Nuclear cortical migration is initiated at telophase of cycle 9 and ends about 1.5 minutes into early interphase of cycle 10 in embryos with two extra copies of the maternal CycB⁺ gene dose (derived from females carrying copies of CycB^+t10) and carrying Sse^13m, as occurs in wild-type embryos, but the velocity and pattern of nuclear migration are abnormal; nuclear movements are faster than in wild-type embryos and the nuclei move in curved paths and at an angle to the cortex (the nuclei migrate in straight paths perpendicular to the cortex in wild-type embryos). The reduction in the microtubule network (measured during early interphase of cycle 9) seen in embryos with four extra copies of the maternal CycB⁺ gene dose (derived from females carrying copies of CycB^+t10) is suppressed by Sse^13m/+. The loss of astral microtubules seen in cycle 9 embryos with four extra copies of the maternal CycB⁺ gene dose is enhanced if they also carry Sse^13m/+.

(Ji et al., 2005)

The maternal effect lethality and cellularisation defects of embryos derived from females carrying two copies of thr^{ΔVQ.T:Hsap\MYC} and raised at 18^oC are suppressed by one copy of Sse^13m; only 7% of these embryos have cellularisation defects. If Sse^{C497S.Scer\UAS.T:Ivir\HA1} is expressed in this background under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} the frequency and severity of cellularisation defects is unaffected. If, however, Sse^{Scer\UAS.T:Ivir\HA1} is expressed in this background under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}, 56% of the embryos have cellularisation defects.

(Herzig et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Sse^13m is rescued by Sse^+t10

(Jager et al., 2001)

Partially rescued by

Sse^13m is partially rescued by Sse^UAS.Tag:HA/Scer\GAL4^da.G32

(Jager et al., 2001)

Not rescued by

Sse^13m is not rescued by Sse^{C497S.UAS.Tag:HA}/Scer\GAL4^da.G32

(Jager et al., 2001)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8988

mwh¹ Sse^13m red¹ e¹/TM6B, Tb⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

13m281

Sse^13m

(Blattner et al., 2016, Dorsett et al., 2005)

l(3)13m-281

(Cipressa et al., 2016, Gatti and Goldberg, 1991)

l(3)SG14¹

(Gatti and Baker, 1989)

sse^13m

(Ji et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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