Open Close
General Information
Symbol
Dmel\Invadolysin1
Species
D. melanogaster
Name
FlyBase ID
FBal0011206
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

l(3)IX-141 mutant third instar larvae have a much-reduced brain size and missing imaginal discs.

l(3)IX-141 mutant neuroblasts from third instar larval brains have mitotic chromosomes that are hypercondensed length-wise but appear loosely compacted with a ragged periphery. This phenotype is not rescued by allowing more time in mitosis with colchicine treatment.

l(3)IX-141 mutant polytene chromosomes from third instar larval salivary glands lack their distinctive banding, their chromosome arms appear twisted and frayed, and they are approximately half the size of wild type. It is difficult to identify an obvious chromocentre in many l(3)IX-141 mutant nuclei.

BrdU incorporation is clearly reduced in l(3)IX-141 mutant neuroblasts from third instar larval brains.

Larval neuroblasts of l(3)IX-141 mutants exhibit abnormal spindle morphology, with only 2% of mitotic cells having a normal bipolar spindle, 37% having a monopolar spindle, 34% having a disorganised spindle, and 27% having a bipolar asymmetric spindle. Mutant spindles also appear to have thicker bundles of microtubules compared to wild type. Almost no anaphase mitotic figures are observed in mutant neuroblasts, consistent with a metaphase delay or arrest.

Nearly 70% of mitotic figures in l(3)IX-141 mutant neuroblasts appear to have only one focus of centrosomal staining, often having a dumbbell shape suggesting that centrosomes have duplicated but not separated.

Germ cells fail to migrate and coalesce in l(3)IX-141 mutant embryos and mutant larvae lack gonads.

Imaginal discs of homozygous larvae are small. Defects in the cell cycle: few or no dividing cells, affects chromosome condensation and arrest of the cell cycle at metaphase.

Lethality acts at second-third instar larval stage. Fuzzy, swollen mitotic chromosomes; chromosome breaks.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
IX-14
Invadolysin1
ads1
Name Synonyms
Secondary FlyBase IDs
    References (5)