Imaginal discs of homozygous larvae are small. Defects in the cell cycle: few or no dividing cells and affects chromosome condensation.
More than 50% of homozygous larvae form puparia, although none of them metamorphose. Mutant larvae have smaller imaginal discs than normal (the maximum size is smaller than the size of wild-type late second instar discs) which cannot differentiate into adult structures when injected into metamorphosing normal larvae. The eye-antenna, leg, wing, haltere and genital discs are abnormal. Late second instar mutant wing discs and eye-antenna-brain complexes injected into wild-type adult females decrease in size for the first 3 days, after which they are no longer detectable. Wings discs from early third instar wild-type larvae injected into late second or early third instar mutant larvae show reduced growth compared to control wild-type discs injected into wild-type larvae. These discs can differentiate when injected into metamorphosing larvae.