FlyBase Allele Report: Dmel\Med[1]

General Information

Symbol

Dmel\Med¹

Species

D. melanogaster

Name

FlyBase ID

FBal0011292

Feature type

allele

Associated gene

Dmel\Med

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

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Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

point_mutation

3R:31,613,045..31,613,045 [+]

Nucleotide change:

C31613045T

Amino acid change:

Q283term | Med-PA; Q283term | Med-PC

Reported amino acid change:

Q283term

Comment:

Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.

(Das et al., 1998)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Polytene chromosomes apparently normal.

Nature of the lesion

Statement

Reference

Amino acid replacement: Q283term.

(Das et al., 1998)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cell cycle

(Gatti and Baker, 1989)

denticle belt

(Das et al., 1998)

embryonic/first instar larval cuticle

(Das et al., 1998)

eye | somatic clone

(Das et al., 1998)

imaginal disc

(Gatti and Baker, 1989)

imaginal disc | larval stage

(Gatti and Baker, 1989)

larval head | embryonic stage

(Das et al., 1998)

Detailed Description

Statement

Reference

Med¹ zygotic mutant embryos occasionally have dorsal defects.

(Newfeld and Takaesu, 2002)

Mutant stage 17 embryos do not show significant defects in the location or formation of the tracheal dorsal trunk branch, dorsal branch 10, spiracular branch 10 or the posterior spiracle.

(Takaesu et al., 2002)

Embryos lacking maternal and zygotic Med function (Med¹/Med²⁵ embryos derived from females with homozygous Med¹ germ line clones) show loss of dorsal tissue, expansion of the lateral denticle bands into dorsal regions and severe head defects. The phenotype is partially paternally rescuable. Homozygous clones in the eye are only seen at the margins of the eye, most commonly at the posterior margin, and result in loss of eye tissue.

(Das et al., 1998)

Late larval early pupal lethality. Reduction in the number of mitosis in the larval brain (FBrf0049822).

(Raftery et al., 1995)

Imaginal discs of homozygous larvae are missing or degenerate. Defects in the cell cycle: few or no dividing cells and affects chromosome condensation.

(Gatti and Baker, 1989)

imaginal discs missing weak effect on mitotic chromosome condensation Homozygous larvae contain rudimentary imaginal discs, but disc primordia do not grow during larval development; testes and ovaries smaller than normal and cell number in central nervous system reduced. Mutant gonads do not survive metamorphosis when implanted into wild-type larvae. Homozygous cuticular clones appear to develop normally, but with reduced frequency and size compared to control clones. Mutant larvae support growth of implanted wild-type discs. Normal gene product postulated to be required for cell proliferation; survival of somatic epidermal clones attributed to perdurance. Larval ganglion mitoses exhibit weak effect on chromosome condensation as well as chromosome breakage (Gatti and Baker, 1989);

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Med¹ has lethal | recessive phenotype, non-enhanceable by lilli^unspecified

(Su et al., 2001)

Suppressor of

Statement

Reference

Med[+]/Med¹ is a suppressor of visible phenotype of Scer\GAL4^A9, gbb^UAS.cKa

(Bangi and Wharton, 2006)

Med[+]/Med¹ is a suppressor of visible phenotype of Scer\GAL4^A9, dpp^UAS.cHa

(Bangi and Wharton, 2006)

Med[+]/Med¹ is a suppressor of visible phenotype of tkv^SC143

(Das et al., 1998)

Med[+]/Med¹ is a suppressor of visible phenotype of Scer\GAL4^en-e16E, sax^{Q263D.UAS.cDa}

(Das et al., 1998)

Other

Statement

Reference

Med¹, dpp^hr27/dpp[+] has lethal | dominant | maternal effect phenotype

(Das et al., 1998)

Med[+]/Med¹, dpp^hr27 has lethal | dominant phenotype

(Das et al., 1998)

Phenotype Manifest In

Enhancer of

Statement

Reference

Med¹ is an enhancer of phenotype of dpp^hr4

(Raftery et al., 1995)

Suppressor of

Statement

Reference

Med[+]/Med¹ is a suppressor of wing vein phenotype of Scer\GAL4^A9, gbb^UAS.cKa

(Bangi and Wharton, 2006)

Med[+]/Med¹ is a suppressor of wing phenotype of Scer\GAL4^A9, dpp^UAS.cHa

(Bangi and Wharton, 2006)

Med[+]/Med¹ is a suppressor of wing phenotype of tkv^SC143

(Das et al., 1998)

Med[+]/Med¹ is a suppressor of wing | posterior phenotype of Scer\GAL4^en-e16E, sax^{Q263D.UAS.cDa}

(Das et al., 1998)

Med[+]/Med¹ is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^en-e16E, sax^{Q263D.UAS.cDa}

(Das et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Slightly suppresses the wing phenotype produced by tkv^SC143. Shows a dominant maternal effect interaction with dpp; when Med¹/+ females are crossed to dpp^hr27/+ males, all progeny carrying dpp^hr27 die. This lethality is rescued by Med^Ubi-p63E.PD also partly by Mad^{Ubi-p63E.T:Hsap\MYC}.