Allele Dmel\Med¹

General Information
Symbol	Dmel\Med¹	Species	D. melanogaster
Name		FlyBase ID	FBal0011292
Feature type	allele	Created / Updated	2006-08-22/2006-08-22
Associated gene	Dmel\Med

Allele class
Mutagen	ethyl methanesulfonate
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: Q283@. (Das et al., 1998)
Assay mode
Cytology	Polytene chromosomes apparently normal.
Phenotypic Data
Phenotypic Class
	lethal (with Med⁵) (Cook, 2006.1.31) lethal (with Med¹³) (Cook, 2006.1.31) lethal \| recessive (Raftery et al., 1995) lethal \| larval stage \| recessive (Gatti and Baker, 1989) visible \| recessive (Das et al., 1998) mitotic cell cycle defective (Gatti and Baker, 1989)
Phenotype Manifest In
	imaginal disc (Gatti and Baker, 1989) cell cycle (Gatti and Baker, 1989) embryonic/first instar larval cuticle (Das et al., 1998) denticle belt (Das et al., 1998) larval head & embryo (Das et al., 1998) eye \| somatic clone (Das et al., 1998)
Detailed Description
	Statement Reference imaginal discs missing weak effect on mitotic chromosome condensation Homozygous larvae contain rudimentary imaginal discs, but disc primordia do not grow during larval development; testes and ovaries smaller than normal and cell number in central nervous system reduced. Mutant gonads do not survive metamorphosis when implanted into wild-type larvae. Homozygous cuticular clones appear to develop normally, but with reduced frequency and size compared to control clones. Mutant larvae support growth of implanted wild-type discs. Normal gene product postulated to be required for cell proliferation; survival of somatic epidermal clones attributed to perdurance. Larval ganglion mitoses exhibit weak effect on chromosome condensation as well as chromosome breakage (Gatti and Baker, 1989); Imaginal discs of homozygous larvae are missing or degenerate. Defects in the cell cycle: few or no dividing cells and affects chromosome condensation. (Gatti and Baker, 1989) Late larval early pupal lethality. Reduction in the number of mitosis in the larval brain (FBrf0049822). (Raftery et al., 1995) Embryos lacking maternal and zygotic Med function (Med¹/Med²⁵ embryos derived from females with homozygous Med¹ germ line clones) show loss of dorsal tissue, expansion of the lateral denticle bands into dorsal regions and severe head defects. The phenotype is partially paternally rescuable. Homozygous clones in the eye are only seen at the margins of the eye, most commonly at the posterior margin, and result in loss of eye tissue. (Das et al., 1998) Mutant stage 17 embryos do not show significant defects in the location or formation of the tracheal dorsal trunk branch, dorsal branch 10, spiracular branch 10 or the posterior spiracle. (Takaesu et al., 2002) Med¹ zygotic mutant embryos occasionally have dorsal defects. (Newfeld and Takaesu, 2002)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement Reference Med¹ has lethal \| recessive phenotype, non-enhanceable by lilli^unspecified (Su et al., 2001)
Suppressor of
Statement Reference Med[+]/Med¹ is a suppressor of visible phenotype of tkv^SC143 (Das et al., 1998) Med[+]/Med¹ is a suppressor of visible phenotype of Scer\GAL4^en-e16E, sax^{Q263D.Scer\UAS.cDa} (Das et al., 1998) Med[+]/Med¹ is a suppressor of visible phenotype of Scer\GAL4^A9, gbb^Scer\UAS.cKa (Bangi and Wharton, 2006) Med[+]/Med¹ is a suppressor of visible phenotype of Scer\GAL4^A9, dpp^Scer\UAS.cHa (Bangi and Wharton, 2006)
Other
Statement Reference Med¹, dpp^hr27/dpp[+] has lethal \| maternal effect \| dominant phenotype (Das et al., 1998) Med[+]/Med¹, dpp^hr27 has lethal \| dominant phenotype (Das et al., 1998)
Phenotype Manifest In
Enhancer of
Statement Reference Med¹ is an enhancer of phenotype of dpp^hr4 (Raftery et al., 1995)
Suppressor of
Statement Reference Med[+]/Med¹ is a suppressor of wing phenotype of tkv^SC143 (Das et al., 1998) Med[+]/Med¹ is a suppressor of wing \| posterior phenotype of Scer\GAL4^en-e16E, sax^{Q263D.Scer\UAS.cDa} (Das et al., 1998) Med[+]/Med¹ is a suppressor of wing vein \| ectopic phenotype of Scer\GAL4^en-e16E, sax^{Q263D.Scer\UAS.cDa} (Das et al., 1998) Med[+]/Med¹ is a suppressor of wing vein phenotype of Scer\GAL4^A9, gbb^Scer\UAS.cKa (Bangi and Wharton, 2006) Med[+]/Med¹ is a suppressor of wing phenotype of Scer\GAL4^A9, dpp^Scer\UAS.cHa (Bangi and Wharton, 2006)
Additional Comments
Genetic Interactions
Statement Reference Maternal lethal interaction with dpp^hr4 is due to a loss of dorsal-most fates in the embryos, demonstrated by loss of amnioserosa cells. (Raftery et al., 1995) Slightly suppresses the wing phenotype produced by tkv^SC143. Shows a dominant maternal effect interaction with dpp; when Med¹/+ females are crossed to dpp^hr27/+ males, all progeny carrying dpp^hr27 die. This lethality is rescued by Med^Ubi-p63E.PD also partly by Mad^{Ubi-p63E.T:Hsap\MYC}. (Das et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Med⁵ (Cook, 2006.1.31) Med²⁶ (Das et al., 1998) Med²⁵ (Das et al., 1998) Med²³ (Das et al., 1998) Med²¹ (Das et al., 1998) Med¹⁹ (Das et al., 1998) Med¹³ (Cook, 2006.1.31)
Comments
Stocks ( 1 )
Bloomington	9033 mwh¹ Med¹ e¹/TM3, Sb¹ Ser¹
Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	l(3)12m137 l(3)SG70¹ med¹ (Dequier et al., 2001) Med¹ (Bangi and Wharton, 2006)
Name Synonym
Secondary FlyBase IDs

References ( 14 )
Research paper	Bangi and Wharton, 2006, Development 133(17): 3295--3303 Dual function of the Drosophila Alk1/Alk2 ortholog Saxophone shapes the Bmp activity gradient in the wing imaginal disc. [FBrf0194511] Johnson et al., 2003, Dev. Biol. 262(1): 137--151 Embryonic enhancers in the dpp disk region regulate a second round of Dpp signaling from the dorsal ectoderm to the mesoderm that represses Zfh-1 expression in a subset of pericardial cells. [FBrf0173104] Sutherland et al., 2003, Development 130(23): 5705--5716 Stepwise formation of a SMAD activity gradient during dorsal-ventral patterning of the Drosophila embryo. [FBrf0167503] Newfeld and Takaesu, 2002, Genetics 161(2): 685--692 An analysis using the hobo genetic system reveals that combinatorial signaling by the Dpp and Wg Pathways regulates dpp expression in leading edge cells of the dorsal ectoderm in Drosophila melanogaster. [FBrf0149021] Takaesu et al., 2002, Dev. Biol. 247(2): 225--236 Combinatorial signaling by an unconventional Wg Pathway and the Dpp pathway requires Nejire (CBP/p300) to regulate dpp expression in posterior tracheal branches. [FBrf0148942] Dequier et al., 2001, Mech. Dev. 106(1-2): 47--60 Top-DER- and Dpp-dependent requirements for the Drosophila fos/kayak gene in follicular epithelium morphogenesis. [FBrf0137300] Su et al., 2001, Genetics 157(2): 717--725 A screen for modifiers of decapentaplegic mutant phenotypes identifies lilliputian, the only member of the Fragile-X/Burkitt's Lymphoma family of transcription factors in Drosophila melanogaster. [FBrf0132343] Jazwinska et al., 1999, Development 126(15): 3323--3334 The role of brinker in mediating the graded response to Dpp in early Drosophila embryos. [FBrf0109999] Das et al., 1998, Development 125(8): 1519--1528 The Drosophila gene Medea demonstrates the requirement for different classes of Smads in dpp signaling. [FBrf0102615] Wisotzkey et al., 1998, Development 125(8): 1433--1445 Medea is a Drosophila Smad4 homolog that is differentially required to potentiate DPP responses. [FBrf0102610] Raftery et al., 1995, Genetics 139(1): 241--254 Genetic screens to identify elements of the decapentaplegic signaling pathway in Drosophila. [FBrf0079237] Gatti and Baker, 1989, Genes Dev. 3: 438--453 Genes controlling essential cell-cycle functions in Drosophila melanogaster. [FBrf0049822]
Personal communication to FlyBase	Cook, 2006.1.31, Characterization of deletions and Minutes in chromosomal region 99-100. Characterization of deletions and Minutes in chromosomal region 99-100. [FBrf0191633] Raftery, 1995.4.6, [title not yet available] [title not yet available] [FBrf0086254]

Allele Dmel\Med1

Allele Dmel\Med¹