Allele Dmel\pho¹

General Information
Symbol	Dmel\pho¹	Species	D. melanogaster
Name		FlyBase ID	FBal0011501
Feature type	allele	Associated gene	Dmel\pho
Also Known As	29

Map ( GBrowse )	Untitled Document
Allele class	hypomorphic allele - genetic evidence
Mutagen	natural population, spontaneous
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Brown et al., 1998)
Mutagen	natural population (Hochman et al., 1964) spontaneous (Hochman, 1971, Hochman, 1974, Hochman, 1976)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 4:1,194,972..1,194,974 (Brown et al., 1998)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference 4.5kb Doc insertion into the coding region at codon 272, upstream of the zinc finger region of the protein. (Brown et al., 1998)
Caused by insertion	Doc{}pho¹ (Brown et al., 1998)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| pharate adult stage \| recessive (Brown and Kassis, 2010) lethal \| pupal stage (Lindsley and Zimm, 1992) lethal \| pupal stage \| recessive (Poux et al., 2001, Hochman, 1974, Hochman, 1976) lethal \| recessive (Hochman, 1971, Hochman et al., 1964)
Phenotype Manifest In
	antenna (with pho^cv) (Atchison et al., 2003) sex comb (with pho^cv) (Atchison et al., 2003) unguis (with pho^cv) (Atchison et al., 2003)
Detailed Description
	Statement Reference Heterozygotes do not have extra sex combs on the 2nd or 3rd leg and do not show transformation of antenna to leg. (Busturia et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference pho¹ has lethal \| recessive \| pharate adult stage phenotype, enhanceable by Spps¹/Spps¹ (Brown and Kassis, 2010)
Enhancer of
Statement Reference pho[+]/pho¹ is an enhancer of visible phenotype of E2f^Scer\UAS.cNa, Scer\GAL4^Act88F.PD (Ji et al., 2012) pho¹ is an enhancer of visible phenotype of Trl^R85, Ubx¹³⁰ (Bejarano and Busturia, 2004)
NOT Enhancer of
Statement Reference pho[+]/pho¹ is a non-enhancer of visible phenotype of Dp^Scer\UAS.cDa, E2f^Scer\UAS.cNa, Scer\GAL4^GMR.PU (Ji et al., 2012)
Suppressor of
Statement Reference pho[+]/pho¹ is a suppressor \| partially of visible phenotype of E2f^{dsRNA.Scer\UAS.cJa}, Scer\GAL4^ptc-559.1 (Ji et al., 2012) pho[+]/pho¹ is a suppressor of eye color defective phenotype of w^Mcp.13, w^Mcp.scs'.14 (Muller et al., 1999) pho[+]/pho¹ is a suppressor of visible phenotype of E2f^{dsRNA.Scer\UAS.cJa}, Scer\GAL4^GMR.PU (Ji et al., 2012) pho¹ is a suppressor of eye color defective phenotype of w^Fab-7.5F24 (Cavalli, 1999) pho^cv/pho¹ is a suppressor of eye color defective phenotype of P{iab-7PRE-w.860}P24, w^iab-7PRE.860 (Mishra et al., 2001) pho^cv/pho¹ is a suppressor of eye color defective phenotype of w^Mcp.10 (Muller et al., 1999) pho^cv/pho¹ is a suppressor of eye color defective phenotype of w^Mcp.13 (Muller et al., 1999) pho^cv/pho¹ is a suppressor of eye color defective phenotype of w^Mcp.scs'.11 (Muller et al., 1999) pho^cv/pho¹ is a suppressor of eye color defective phenotype of w^Mcp.scs'.13, w^Mcp.scs'.14 (Muller et al., 1999)
NOT Suppressor of
Statement Reference pho[+]/pho¹ is a non-suppressor of visible phenotype of Dp^Scer\UAS.cDa, E2f^Scer\UAS.cNa, Scer\GAL4^GMR.PU (Ji et al., 2012) pho^cv/pho¹ is a non-suppressor of eye color defective phenotype of w^Mcp.11 (Muller et al., 1999) pho^cv/pho¹ is a non-suppressor of eye color defective phenotype of w^Mcp.scs'.14 (Muller et al., 1999)
Other
Statement Reference pho¹, phol^81A has lethal \| larval stage phenotype (Brown et al., 2003, Brown et al., 2003) Spps¹, pho¹ has body size defective \| larval stage phenotype (Brown and Kassis, 2010) Trl^R85, pho[+]/pho¹ has visible \| homeotic phenotype (Busturia et al., 2001) Trl^R85, pho¹ has visible \| homeotic phenotype (Busturia et al., 2001)
Phenotype Manifest In
Enhanced by
Statement Reference pho¹ has phenotype, enhanceable by E(Pc)¹ (Lindsley and Zimm, 1992)
Suppressed by
Statement Reference pho^cv/pho¹ has antenna phenotype, suppressible by Hsap\YY1^{Hsp70.T:Scer\GAL4} (Atchison et al., 2003) pho^cv/pho¹ has sex comb phenotype, suppressible by Hsap\YY1^{Hsp70.T:Scer\GAL4} (Atchison et al., 2003) pho^cv/pho¹ has unguis phenotype, suppressible \| partially by Hsap\YY1^{Hsp70.T:Scer\GAL4} (Atchison et al., 2003)
NOT suppressed by
Statement Reference pho^cv/pho¹ has antenna phenotype, non-suppressible by Hsap\YY1^arm.PA (Atchison et al., 2003) pho^cv/pho¹ has sex comb phenotype, non-suppressible by Hsap\YY1^arm.PA (Atchison et al., 2003) pho^cv/pho¹ has unguis phenotype, non-suppressible by Hsap\YY1^arm.PA (Atchison et al., 2003)
Enhancer of
Statement Reference pho[+]/pho¹ is an enhancer of wing phenotype of E2f^Scer\UAS.cNa, Scer\GAL4^Act88F.PD (Ji et al., 2012)
NOT Enhancer of
Statement Reference pho[+]/pho¹ is a non-enhancer of eye phenotype of Dp^Scer\UAS.cDa, E2f^Scer\UAS.cNa, Scer\GAL4^GMR.PU (Ji et al., 2012)
Suppressor of
Statement Reference pho[+]/pho¹ is a suppressor \| partially of 1st posterior cell phenotype of E2f^{dsRNA.Scer\UAS.cJa}, Scer\GAL4^ptc-559.1 (Ji et al., 2012) pho[+]/pho¹ is a suppressor \| partially of adult abdominal segment 4 \| ectopic phenotype of E(z)^Trm (Bajusz et al., 2001) pho[+]/pho¹ is a suppressor \| partially of adult abdominal segment 5 phenotype of E(z)^Trm (Bajusz et al., 2001) pho[+]/pho¹ is a suppressor \| partially of adult mesothoracic segment \| ectopic phenotype of E(z)^Trm (Bajusz et al., 2001) pho[+]/pho¹ is a suppressor \| partially of adult metathoracic segment phenotype of E(z)^Trm (Bajusz et al., 2001) pho[+]/pho¹ is a suppressor \| partially of adult prothoracic segment phenotype of E(z)^Trm (Bajusz et al., 2001) pho[+]/pho¹ is a suppressor of eye phenotype of E2f^{dsRNA.Scer\UAS.cJa}, Scer\GAL4^GMR.PU (Ji et al., 2012) pho[+]/pho¹ is a suppressor of pigment cell phenotype of w^Mcp.13, w^Mcp.scs'.14 (Muller et al., 1999) pho¹ is a suppressor of phenotype of w^en.8 (Brown et al., 1998) pho¹ is a suppressor of pigment cell phenotype of w^Fab-7.5F24 (Cavalli, 1999) pho^cv/pho¹ is a suppressor of pigment cell phenotype of w^Mcp.13 (Muller et al., 1999) pho^cv/pho¹ is a suppressor of pigment cell phenotype of w^Mcp.scs'.11 (Muller et al., 1999) pho^cv/pho¹ is a suppressor of pigment cell phenotype of w^Mcp.scs'.13, w^Mcp.scs'.14 (Muller et al., 1999)
NOT Suppressor of
Statement Reference pho[+]/pho¹ is a non-suppressor of eye phenotype of Dp^Scer\UAS.cDa, E2f^Scer\UAS.cNa, Scer\GAL4^GMR.PU (Ji et al., 2012) pho^cv/pho¹ is a non-suppressor of pigment cell phenotype of w^Mcp.11 (Muller et al., 1999) pho^cv/pho¹ is a non-suppressor of pigment cell phenotype of w^Mcp.scs'.14 (Muller et al., 1999)
Other
Statement Reference E(Pc)¹, pho¹ has embryonic head phenotype (Lindsley and Zimm, 1992) pho¹, pho^cv, w^Mcp.10 has pigment cell phenotype (Muller et al., 1999) pho¹, phol^81A has embryonic/larval brain phenotype (Brown et al., 2003, Brown et al., 2003) pho¹, phol^81A has imaginal disc phenotype (Brown et al., 2003, Brown et al., 2003) pho¹, phol^81A has polytene chromosome phenotype (Brown et al., 2003, Brown et al., 2003) Spps¹, pho¹ has imaginal disc phenotype (Brown and Kassis, 2010) Trl^R85, pho[+]/pho¹ has mesothoracic leg phenotype (Busturia et al., 2001) Trl^R85, pho[+]/pho¹ has metathoracic leg phenotype (Busturia et al., 2001) Trl^R85, pho[+]/pho¹ has sex comb \| ectopic phenotype (Busturia et al., 2001) Trl^R85, pho¹ has mesothoracic leg phenotype (Busturia et al., 2001) Trl^R85, pho¹ has metathoracic leg phenotype (Busturia et al., 2001) Trl^R85, pho¹ has sex comb \| ectopic phenotype (Busturia et al., 2001)
Additional Comments
Genetic Interactions
Statement Reference pho[1], Spps[1] double mutants die earlier in development than either single mutant: single mutants die as pharates, whereas double mutants die as third instar larvae or often at earlier stages. Double mutant larvae are often abnormally small and have misshapen imaginal discs. (Brown and Kassis, 2010) The reduced wing phenotype of RYBP[Scer\UAS.cBa]; Scer\GAL4[sd-SG29.1] flies is enhanced by pho[1]/+. (Bejarano et al., 2005) Trl^R85/Ubx¹³⁰; pho¹ flies show an enhancement of the Ubx¹³⁰ phenotype in 18% of flies. (Bejarano and Busturia, 2004) phol^81A ; pho¹ double mutants die as third instar larvae and fail to pupate. The brain is smaller than normal, the disks are misshapen and smaller than normal and the salivary gland polytene chromosomes are enlarged in double mutant third instar larvae. (Brown et al., 2003) 11% of Trl^R85/+ ; pho¹/+ double heterozygotes have extra sex combs (on the 2nd or 3rd leg) but they do not show transformation of antenna to leg. (Busturia et al., 2001) pho¹/pho^cv suppresses the pairing-dependent silencing of w^iab-7PRE.860 seen in flies homozygous for P{iab-7PRE-w.860}P24. (Mishra et al., 2001) Mutant heterozygotes show a moderate suppression of the eye phenotype see in P{UAS-lacZ.Abd-B.5F24} leading to darker eyes. (Cavalli, 1999) The addition of pho¹/pho^cv strongly suppresses the Pairing sensitive silencing effect seen in w^Mcp.10, w^Mcp.13,w^{Mcp.scs'.12:15} and w^Mcp.scs'.11. The addition of pho¹/pho^cv has no effect on the Pairing sensitive silencing effect seen in w^Mcp.11, and w^Mcp.scs'.14. The addition of pho¹/pho^cv very strongly suppresses the Pairing sensitive silencing effect seen in w^Mcp.scs'.11 (P{Mcp-w#11}16/+, P{Mcp-w#11}102/+) double heterozygous insertions. The addition pho¹/pho^cv very strongly suppresses the Pairing sensitive silencing effect seen in the w^Mcp.scs'.14/ w^Mcp.13 (P{Mcp-w#14}AP-2^stummelbein/+, P{Mcp-w#13}116/+) double heterozygous insertion. (Muller et al., 1999) Has little or no effect on the phenotype of E2f^GMR.PD Dp^GMR.PD BacA\p35^GMR.PH flies. (Staehling-Hampton et al., 1999) Eye colour encoded by w^en.8 is darker in pho¹/pho¹ or pho¹/pho^cv background. (Brown et al., 1998)
Xenogenetic Interactions
Statement Reference When heatshocked twice daily, Hsap\YY1^{Hsp70.T:Scer\GAL4} nearly completely corrects the antennal and sex comb defects in pho¹/pho^cv mutant flies. Segmentation is largely normal, although pigmentation on tergite 5 is not always complete. Correction of claw structures is variable ranging from complete to partial correction. (Atchison et al., 2003)
Complementation & Rescue Data
Rescued by	pho¹ is rescued by pho^{αTub84B.T:Ecol\lexA} (Poux et al., 2001) pho¹ is rescued by pho^{αTub84B.T:Zzzz\TAP} (Klymenko et al., 2006)
Partially rescued by	pho¹ is partially rescued by pho^{hs.T:Ecol\lexA} (Poux et al., 2001)
Comments	Two copies of pho^{hs.T:Ecol\lexA} produces healthy pho¹/pho¹ adults with one heat shock daily beginning 4 days after egg laying. The rescued pho¹/pho¹ males are fully fertile, but occasionally show sex comb bristles on the mesothoracic legs and excess pigmentation in the 4th abdominal tergite. The rescued pho¹/pho¹ females have severely impaired fertility that cannot be rescued with a paternal pho⁺ allele. Only 10% of embryos derived from pho¹/pho¹ females crossed to pho¹/pho¹ males hatch, even when heat shocked before blastoderm to express pho^{hs.T:Ecol\lexA}. These embryos show frequent and various segmental abnormalities but never show classical homeotic derepression phenotypes. The few larvae that hatch grow slowly and never reach the pupal stage, probably because evident head skeleton abnormalities preclude normal feeding. (Poux et al., 2001)
Stocks ( 3 )
Bloomington	6212 pho¹/In(4)ci^D, ci^D pan^ciD 16111 y¹ w¹¹¹⁸; PBac{5HPw⁺}Syt7^A426/pho¹
Kyoto	103320 ci^D / pho¹
Notes on Origin
Discoverer
	Isolated from: Bountiful, UT population. (Hochman et al., 1964)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	29 (Hochman, 1976, Hochman, 1974, Hochman, 1971) l(4)29 (Lindsley and Zimm, 1992) l(4)102EFc¹ (Lindsley and Zimm, 1992) l(4)BU-2 (Hochman et al., 1964) pho^1(l(4)29) (Rusch and Kaufman, 2000) pho¹ (Klymenko et al., 2006, Goodliffe et al., 2007, Ji et al., 2012, Hirose et al., 2001, Bejarano et al., 2005, González et al., 2008, Brown and Kassis, 2010)
Name Synonym
Secondary FlyBase IDs

References ( 28 )

Generate a list of	All references relating to this allele ( 28 )

List References by type	Research paper ( 24 ) Review ( 2 ) Personal communication to FlyBase ( 1 ) Book ( 1 )
Recent research papers ( 1 )
	Ji et al., 2012, G3 (Bethesda) 2(12): 1651--1660 In Vivo Regulation of E2F1 by Polycomb Group Genes in Drosophila. [FBrf0220353] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...

Allele Dmel\pho1

Allele Dmel\pho¹