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General Information
Symbol
Dmel\lace2
Species
D. melanogaster
Name
FlyBase ID
FBal0011550
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
laceHG34, HG34
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G15503198A

Amino acid change:

S429N | lace-PA; S429N | lace-PB

Reported amino acid change:

S429N

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: S429N.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Unlike wild-type and lace[2] heterozygous controls, lacek05305/lace2 transheterozygous adults show a mushroom body "lost-lobe" phenotype in about half of the cases; the overall dendrite organization seems unaffected, as indicated by microtubule polarity (nod) and compartment identity (Apc2, DenMark, etc). Single cell analysis reveals a failure of lacek05305/lace2 mushroom body neurons to extend their axon branches dorsally, instead developing parallel horizontal branches. In lacek05305/lace2 larvae and pupae the vertical α/α' Kenyon cell lobe is already missing. Despite the missing lobe in larvae, the α/β axons and α'/β' axons still segregate into distinct horizontal lobes; there is non-segregation of the α' and β' growth cones, despite of unperturbed peduncle development; γ Kenyon cells do not seem to be affected.

lace2/lacek05305 larvae have significantly reduced bouton number and significantly increased bouton size at the neuromuscular junction of muscle 4, compared to controls, with no effect on the number of satellite boutons.

lace2 mutant wing disc clones generated in a Minute background show significant tissue overgrowth. Overgrowth phenotypes are also seen when lace2 mutant clones are generated in the posterior compartment of the wing disc. These are seen in the wing disc pouch and hinge regions but not in the notum region. Increased proliferation is seen compared to controls but apicobasal polarity is unaffected.

lacek05305/lace2 transheterozygous mutant adults present significantly decreased survival to starvation, decreased body weight, decreased adiposity in abdominal sections and decreased sizes of fat body cells and lipid droplets, as compared to controls.

lacek05305/lace2 transheterozygous mutant adults present similar feeding behavior to controls, as assessed by starvation-induced appetite, postprandial meal volume, average meal frequency and volume, and caloric intake, as compared to controls.

The reduction in electroretinogram (ERG) amplitude seen in flies exposed to constant light is not significantly affected by lace2/+.

lacek05305/lace2 adults have an average flight score of 1.41 +/- 0.063 compared to 1.62 +/- 0.14 in wild-type flies in a standard flight performance assay. The average number of dorsal longitudinal flight muscles per hemithorax is 5.94 +/- 0.030 in the mutant flies, compared to 6.00 +/- 0.00 in wild-type flies.

lace2 in combination with a pair of introgressions from D.simulans spanning 30F1-31E7 to 35D7-36A14 Dsim\Int(2L)S and 21A1 to 22D1--23A2 Dsim\Int(2L)D produces viable flies.

lacek05305/lace2 shows reduced viability. lacek05305/lace2 flies often show incision of the wing margin, and there is an ectopic crossvein. The hexagonal array of the ommatidia is disrupted, especially along the equatorial plane. The deep pseudopupil pattern is normal. In the notum, the microchaetae lack pigment and are occasionally missing, while the number of macrochaetae is variable. In the leg and antenna, bifurcation of the distal portion is rarely seen. The wing, leg and eye-antennal discs of lacek05305/lace2 animals contain a considerably high number of dead cells. Severe malformation of the primordial wing blade is seen in the wing disc. The developing pupal eye shows loss of cells among cone cells, primary pigment cells, secondary pigment cells, tertiary pigment cells and interommatidial bristles. The lethality and various adult mutant phenotypes of lacek05305/lace2 flies can be rescued by feeding with sphingosine. The adult mutant phenotypes cannot be rescued by exogenous sphingomyelin or ceramide.

Embryo head is malformed.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference

lace[+]/lace2 is an enhancer of neuroanatomy defective | adult stage phenotype of Dscam121/Dscam1[+], Spt-IB2

lace[+]/lace2 is an enhancer of visible phenotype of Scer\GAL4Tub.PU, cswN308D.UASp

lace[+]/lace2 is an enhancer of visible | heat sensitive phenotype of HC2.hs

NOT Suppressor of
Statement
Reference
Phenotype Manifest In
NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Enhancer of
Statement
Reference

lace[+]/lace2 is an enhancer of lobe system of adult mushroom body phenotype of Dscam121/Dscam1[+], Spt-IB2

lace[+]/lace2 is an enhancer of adult olfactory receptor neuron Or47a phenotype of Dscam121/Dscam1[+], Spt-IB2

lace[+]/lace2 is an enhancer of axon | adult stage phenotype of Dscam121/Dscam1[+], Spt-IB2

lace[+]/lace2 is an enhancer of wing vein | ectopic phenotype of Scer\GAL4Tub.PU, cswN308D.UASp

lace[+]/lace2 is an enhancer of wing vein | ectopic | heat sensitive phenotype of HC2.hs

lace[+]/lace2 is an enhancer of wing | heat sensitive phenotype of HC2.hs

Additional Comments
Genetic Interactions
Statement
Reference

Spt-IB2, Dscam121, lace2 triple heterozygous adults show a moderate prevalence of mushroom body defects, namely a "lost-lobe" phenotype.

lace2 heterozygosity somewhat enhances the Or47a neuron axonal mistargeting defects observed in Spt-IB2, Dscam121 double heterozygous adults.

The mushroom body "lost lobe" phenotype penetrance observed in lacek05305/lace2 transheterozygous adults is significantly increased and decreased by the Scer\GAL4Dscam1.4.5-driven expression of Dscam1TM1.UAS.RFP(Unk) and Dscam1miRNA.UAS.17.1, respectively. lace2 heterozygous adults that also express either Dscam1TM1.UAS.RFP(Unk) or Dscam1miRNA.UAS.17.1 under the control of Scer\GAL4Dscam1.4.5 do not show a mushroom body "lost lobe" phenotype.

lacek05305/lace2 transheterozygous and lace2 heterozygous adults that also express Vap33UAS.cPa under the control of Scer\GAL4Tab2-201Y do not show a mushroom body "lost lobe" phenotype.

Expression of ACCScer\UAS.cSa under the control of Scer\GAL4hh.PU does not suppress the overgrowth phenotype seen in lace2 mutant clones in the posterior compartment of the wing disc.

aph-1D35 strongly suppresses the overproliferation seen in lace2 mutant wing disc clones.

Su(H)k07904 substantially suppresses the overproliferation seen in lace2 mutant wing disc clones.

Expression of armS10.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4sd.PU significantly suppresses the overproliferation seen in lace2 mutant wing disc clones, in terms of both frequency and severity of the phenotype.

lacek05305/lace2, Sply05091/Sply05091 adults exhibit a more severe decrease in survival in response to starvation as compared to either lacek05305/lace2 or Sply05091/Sply05091 mutants.

The decreased body weight, decreased adiposity in abdominal sections and decreased sizes of fat body cells and lipid droplets observed in lacek05305/lace2 transheterozygous mutant adults is not altered by Sply05091 homozygosity.

The decreased feeding behavior observed in Sply05091 homozygous mutant adults, as assessed by their decreased starvation-induced appetite, decreased postprandial meal volume, decreased average meal frequency and volume, and decreased daily food intake, is not suppressed by lacek05305/lace2 transheterozygosity.

rlSem suppresses the wing margin incision and loss of microchaetae phenotypes of lacek05305/lace2 flies.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Expression of laceScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4hh.PU rescues the overgrowth phenotype seen in lace2 mutant clones in the posterior compartment of the wing disc.

The lethality of lace11/lace2 flies is rescued by laceScer\UAS.T:Ivir\HA1 expressed under the control of Scer\GAL4Act5C.PI; approximately 50% of these flies survive to the adult stage with no aberrations. Incision of the wing margin in lacek05305/lace2 flies is rescued by laceScer\UAS.T:Ivir\HA1 expressed under the control of Scer\GAL469B. The wing, leg and eye-antennal discs of lacek05305/lace2 animals contain a considerably high number of dead cells. This phenotype is suppressed by hepr75. Severe malformation of the primordial wing blade is seen in the wing disc. The developing pupal eye shows loss of cells among cone cells, primary pigment cells, secondary pigment cells, tertiary pigment cells and interommatidial bristles.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Harrington.

Separable from: l(2)CA5HG34.

Comments
Comments

Phenotypic data included in FBrf0051973.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (21)