The mushroom body "lost lobe" phenotype penetrance observed in lacek05305/lace2 transheterozygous adults is significantly increased and decreased by the Scer\GAL4Dscam1.4.5-driven expression of Dscam1TM1.UAS.RFP(Unk) and Dscam1miRNA.UAS.17.1, respectively. lace2 heterozygous adults that also express either Dscam1TM1.UAS.RFP(Unk) or Dscam1miRNA.UAS.17.1 under the control of Scer\GAL4Dscam1.4.5 do not show a mushroom body "lost lobe" phenotype.
Expression of armS10.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4sd.PU significantly suppresses the overproliferation seen in lace2 mutant wing disc clones, in terms of both frequency and severity of the phenotype.
The decreased body weight, decreased adiposity in abdominal sections and decreased sizes of fat body cells and lipid droplets observed in lacek05305/lace2 transheterozygous mutant adults is not altered by Sply05091 homozygosity.
The decreased feeding behavior observed in Sply05091 homozygous mutant adults, as assessed by their decreased starvation-induced appetite, decreased postprandial meal volume, decreased average meal frequency and volume, and decreased daily food intake, is not suppressed by lacek05305/lace2 transheterozygosity.
The lethality of lace11/lace2 flies is rescued by laceScer\UAS.T:Ivir\HA1 expressed under the control of Scer\GAL4Act5C.PI; approximately 50% of these flies survive to the adult stage with no aberrations. Incision of the wing margin in lacek05305/lace2 flies is rescued by laceScer\UAS.T:Ivir\HA1 expressed under the control of Scer\GAL469B. The wing, leg and eye-antennal discs of lacek05305/lace2 animals contain a considerably high number of dead cells. This phenotype is suppressed by hepr75. Severe malformation of the primordial wing blade is seen in the wing disc. The developing pupal eye shows loss of cells among cone cells, primary pigment cells, secondary pigment cells, tertiary pigment cells and interommatidial bristles.