FlyBase Allele Report: Dmel\lace[2]

Unlike wild-type and lace[2] heterozygous controls, lace^k05305/lace² transheterozygous adults show a mushroom body "lost-lobe" phenotype in about half of the cases; the overall dendrite organization seems unaffected, as indicated by microtubule polarity (nod) and compartment identity (Apc2, DenMark, etc). Single cell analysis reveals a failure of lace^k05305/lace² mushroom body neurons to extend their axon branches dorsally, instead developing parallel horizontal branches. In lace^k05305/lace² larvae and pupae the vertical α/α' Kenyon cell lobe is already missing. Despite the missing lobe in larvae, the α/β axons and α'/β' axons still segregate into distinct horizontal lobes; there is non-segregation of the α' and β' growth cones, despite of unperturbed peduncle development; γ Kenyon cells do not seem to be affected.

(Goyal et al., 2019)

lace²/lace^k05305 larvae have significantly reduced bouton number and significantly increased bouton size at the neuromuscular junction of muscle 4, compared to controls, with no effect on the number of satellite boutons.

(Huang et al., 2018)

lace² mutant wing disc clones generated in a Minute background show significant tissue overgrowth. Overgrowth phenotypes are also seen when lace² mutant clones are generated in the posterior compartment of the wing disc. These are seen in the wing disc pouch and hinge regions but not in the notum region. Increased proliferation is seen compared to controls but apicobasal polarity is unaffected.

(Sasamura et al., 2013)

lace^k05305/lace² transheterozygous mutant adults present significantly decreased survival to starvation, decreased body weight, decreased adiposity in abdominal sections and decreased sizes of fat body cells and lipid droplets, as compared to controls.

lace^k05305/lace² transheterozygous mutant adults present similar feeding behavior to controls, as assessed by starvation-induced appetite, postprandial meal volume, average meal frequency and volume, and caloric intake, as compared to controls.

(Walls et al., 2013)

The reduction in electroretinogram (ERG) amplitude seen in flies exposed to constant light is not significantly affected by lace²/+.

(Lee and Montell, 2004)

lace^k05305/lace² adults have an average flight score of 1.41 +/- 0.063 compared to 1.62 +/- 0.14 in wild-type flies in a standard flight performance assay. The average number of dorsal longitudinal flight muscles per hemithorax is 5.94 +/- 0.030 in the mutant flies, compared to 6.00 +/- 0.00 in wild-type flies.

(Herr et al., 2003)

lace² in combination with a pair of introgressions from D.simulans spanning 30F1-31E7 to 35D7-36A14 Dsim\Int(2L)S and 21A1 to 22D1--23A2 Dsim\Int(2L)D produces viable flies.

(Sawamura et al., 2000)

lace^k05305/lace² shows reduced viability. lace^k05305/lace² flies often show incision of the wing margin, and there is an ectopic crossvein. The hexagonal array of the ommatidia is disrupted, especially along the equatorial plane. The deep pseudopupil pattern is normal. In the notum, the microchaetae lack pigment and are occasionally missing, while the number of macrochaetae is variable. In the leg and antenna, bifurcation of the distal portion is rarely seen. The wing, leg and eye-antennal discs of lace^k05305/lace² animals contain a considerably high number of dead cells. Severe malformation of the primordial wing blade is seen in the wing disc. The developing pupal eye shows loss of cells among cone cells, primary pigment cells, secondary pigment cells, tertiary pigment cells and interommatidial bristles. The lethality and various adult mutant phenotypes of lace^k05305/lace² flies can be rescued by feeding with sphingosine. The adult mutant phenotypes cannot be rescued by exogenous sphingomyelin or ceramide.

(Adachi-Yamada et al., 1999)

Embryo head is malformed.

(Ashburner et al., 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

lace²/lace^k05305 has abnormal neuroanatomy | adult stage phenotype, enhanceable by Scer\GAL4^Dscam1.4.5/Dscam1^{TM1.UAS.RFP(Unk)}

(Goyal et al., 2019)

lace²/lace^k05305 has abnormal starvation stress response | adult stage phenotype, enhanceable by Sply⁰⁵⁰⁹¹/Sply⁰⁵⁰⁹¹

(Walls et al., 2013)

Suppressed by

Statement

Reference

lace²/lace^k05305 has abnormal neuroanatomy | adult stage phenotype, suppressible | partially by Scer\GAL4^Dscam1.4.5/Dscam1^{RNAi.UAS.17.1}

(Goyal et al., 2019)

lace² has increased cell number | somatic clone phenotype, suppressible by aph-1^D35

(Sasamura et al., 2013)

lace² has increased cell number | somatic clone phenotype, suppressible by Su(H)^k07904

(Sasamura et al., 2013)

lace² has increased cell number | somatic clone phenotype, suppressible by Scer\GAL4^sd.PU/arm^{S10.UAS.Tag:MYC}

(Sasamura et al., 2013)

lace²/lace^k05305 has visible phenotype, suppressible by Dsor1^Su1

(Adachi-Yamada et al., 1999)

lace²/lace^k05305 has visible phenotype, suppressible by rl^Sem

(Adachi-Yamada et al., 1999)

NOT suppressed by

Statement

Reference

lace² has increased cell number | somatic clone phenotype, non-suppressible by ACC^UAS.cSa/Scer\GAL4^hh.PU

(Sasamura et al., 2013)

Enhancer of

Statement

Reference

lace[+]/lace² is an enhancer of abnormal neuroanatomy | adult stage phenotype of Dscam1²¹/Dscam1[+], Spt-I^B2

(Goyal et al., 2019)

lace²/lace^k05305 is an enhancer of abnormal starvation stress response | adult stage phenotype of Sply⁰⁵⁰⁹¹

(Walls et al., 2013)

lace[+]/lace² is an enhancer of visible phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

lace[+]/lace² is an enhancer of visible | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

NOT Suppressor of

Statement

Reference

lace²/lace^k05305 is a non-suppressor of abnormal feeding behavior | adult stage phenotype of Sply⁰⁵⁰⁹¹

(Walls et al., 2013)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

lace²/lace^k05305 has adult fat body phenotype, non-enhanceable by Sply⁰⁵⁰⁹¹/Sply⁰⁵⁰⁹¹

(Walls et al., 2013)

lace²/lace^k05305 has lipid droplet | adult stage phenotype, non-enhanceable by Sply⁰⁵⁰⁹¹/Sply⁰⁵⁰⁹¹

(Walls et al., 2013)

Suppressed by

Statement

Reference

lace² has wing disc posterior compartment | somatic clone phenotype, suppressible by aph-1^D35

(Sasamura et al., 2013)

lace² has wing disc posterior compartment | somatic clone phenotype, suppressible by Su(H)^k07904

(Sasamura et al., 2013)

lace² has wing disc posterior compartment | somatic clone phenotype, suppressible by Scer\GAL4^sd.PU/arm^{S10.UAS.Tag:MYC}

(Sasamura et al., 2013)

lace²/lace^k05305 has wing phenotype, suppressible by rl^Sem

(Adachi-Yamada et al., 1999)

lace²/lace^k05305 has microchaeta phenotype, suppressible by rl^Sem

(Adachi-Yamada et al., 1999)

lace²/lace^k05305 has wing disc phenotype, suppressible by hep^r75

(Adachi-Yamada et al., 1999)

lace²/lace^k05305 has wing phenotype, suppressible by Dsor1^Su1

(Adachi-Yamada et al., 1999)

lace²/lace^k05305 has crossvein phenotype, suppressible by Dsor1^Su1

(Adachi-Yamada et al., 1999)

lace²/lace^k05305 has ommatidium phenotype, suppressible by Dsor1^Su1

(Adachi-Yamada et al., 1999)

lace²/lace^k05305 has microchaeta phenotype, suppressible by Dsor1^Su1

(Adachi-Yamada et al., 1999)

lace²/lace^k05305 has macrochaeta phenotype, suppressible by Dsor1^Su1

(Adachi-Yamada et al., 1999)

NOT suppressed by

Statement

Reference

lace² has wing disc posterior compartment | somatic clone phenotype, non-suppressible by ACC^UAS.cSa/Scer\GAL4^hh.PU

(Sasamura et al., 2013)

Enhancer of

Statement

Reference

lace[+]/lace² is an enhancer of lobe system of adult mushroom body phenotype of Dscam1²¹/Dscam1[+], Spt-I^B2

(Goyal et al., 2019)

lace[+]/lace² is an enhancer of adult olfactory receptor neuron Or47a phenotype of Dscam1²¹/Dscam1[+], Spt-I^B2

(Goyal et al., 2019)

lace[+]/lace² is an enhancer of axon | adult stage phenotype of Dscam1²¹/Dscam1[+], Spt-I^B2

(Goyal et al., 2019)

lace[+]/lace² is an enhancer of wing vein | ectopic phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

lace[+]/lace² is an enhancer of wing vein | ectopic | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

lace[+]/lace² is an enhancer of wing | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Spt-I^B2, Dscam1²¹, lace² triple heterozygous adults show a moderate prevalence of mushroom body defects, namely a "lost-lobe" phenotype.

lace² heterozygosity somewhat enhances the Or47a neuron axonal mistargeting defects observed in Spt-I^B2, Dscam1²¹ double heterozygous adults.

The mushroom body "lost lobe" phenotype penetrance observed in lace^k05305/lace² transheterozygous adults is significantly increased and decreased by the Scer\GAL4^Dscam1.4.5-driven expression of Dscam1^{TM1.UAS.RFP(Unk)} and Dscam1^{miRNA.UAS.17.1}, respectively. lace² heterozygous adults that also express either Dscam1^{TM1.UAS.RFP(Unk)} or Dscam1^{miRNA.UAS.17.1} under the control of Scer\GAL4^Dscam1.4.5 do not show a mushroom body "lost lobe" phenotype.

lace^k05305/lace² transheterozygous and lace² heterozygous adults that also express Vap33^UAS.cPa under the control of Scer\GAL4^Tab2-201Y do not show a mushroom body "lost lobe" phenotype.

(Goyal et al., 2019)

Expression of ACC^Scer\UAS.cSa under the control of Scer\GAL4^hh.PU does not suppress the overgrowth phenotype seen in lace² mutant clones in the posterior compartment of the wing disc.

aph-1^D35 strongly suppresses the overproliferation seen in lace² mutant wing disc clones.

Su(H)^k07904 substantially suppresses the overproliferation seen in lace² mutant wing disc clones.

Expression of arm^{S10.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^sd.PU significantly suppresses the overproliferation seen in lace² mutant wing disc clones, in terms of both frequency and severity of the phenotype.

(Sasamura et al., 2013)

lace^k05305/lace², Sply⁰⁵⁰⁹¹/Sply⁰⁵⁰⁹¹ adults exhibit a more severe decrease in survival in response to starvation as compared to either lace^k05305/lace² or Sply⁰⁵⁰⁹¹/Sply⁰⁵⁰⁹¹ mutants.

The decreased body weight, decreased adiposity in abdominal sections and decreased sizes of fat body cells and lipid droplets observed in lace^k05305/lace² transheterozygous mutant adults is not altered by Sply⁰⁵⁰⁹¹ homozygosity.

The decreased feeding behavior observed in Sply⁰⁵⁰⁹¹ homozygous mutant adults, as assessed by their decreased starvation-induced appetite, decreased postprandial meal volume, decreased average meal frequency and volume, and decreased daily food intake, is not suppressed by lace^k05305/lace² transheterozygosity.

(Walls et al., 2013)

rl^Sem suppresses the wing margin incision and loss of microchaetae phenotypes of lace^k05305/lace² flies.

(Adachi-Yamada et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

lace²/lace^k05305 is rescued by Scer\GAL4^Dscam1.4.5/lace^{WT.UAS.Tag:HA}

(Goyal et al., 2019)

lace²/lace^k05305 is rescued by Scer\GAL4^repo.PL/lace^{WT.UAS.Tag:HA}

(Goyal et al., 2019)

lace² is rescued by Scer\GAL4^hh.PU/lace^UAS.Tag:HA

(Sasamura et al., 2013)

lace²/lace^k05305 is rescued by lace^UAS.Tag:HA/Scer\GAL4^69B

(Adachi-Yamada et al., 1999)

lace²/lace¹¹ is rescued by Scer\GAL4^Act5C.PI/lace^UAS.Tag:HA

(Adachi-Yamada et al., 1999)

Partially rescued by

lace²/lace^k05305 is partially rescued by Scer\GAL4^Tab2-201Y/lace^{WT.UAS.Tag:HA}

(Goyal et al., 2019)

Comments

Expression of lace^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hh.PU rescues the overgrowth phenotype seen in lace² mutant clones in the posterior compartment of the wing disc.

(Sasamura et al., 2013)

The lethality of lace¹¹/lace² flies is rescued by lace^{Scer\UAS.T:Ivir\HA1} expressed under the control of Scer\GAL4^Act5C.PI; approximately 50% of these flies survive to the adult stage with no aberrations. Incision of the wing margin in lace^k05305/lace² flies is rescued by lace^{Scer\UAS.T:Ivir\HA1} expressed under the control of Scer\GAL4^69B. The wing, leg and eye-antennal discs of lace^k05305/lace² animals contain a considerably high number of dead cells. This phenotype is suppressed by hep^r75. Severe malformation of the primordial wing blade is seen in the wing disc. The developing pupal eye shows loss of cells among cone cells, primary pigment cells, secondary pigment cells, tertiary pigment cells and interommatidial bristles.

(Adachi-Yamada et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

3159

Adhⁿ⁷ lace² cn¹ vg¹/CyO

Kyoto

106882

Adhⁿ⁷ lace² cn¹ vg¹/CyO

Notes on Origin

Discoverer

Harrington.

Separable from: l(2)CA5^HG34.

(Roote, 1998.1.20)

Comments

Phenotypic data included in FBrf0051973.

(Roote, 1995.5.4)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

HG34

(Adachi-Yamada et al., 1999, Ashburner et al., 1983)

l(2)br36^HG34

(The Moscow Regional Drosophila melanogaster Stock Center, Dubna, Grau et al., 1984)

lace²

(Goyal et al., 2019, Huang et al., 2018, Walls et al., 2018, West et al., 2018, Sasamura et al., 2013, Walls et al., 2013, Roote, 2010.1.4, Geng and MacDonald, 2007, Oishi et al., 2006)

lace^HG34

(Sawamura et al., 2000, Roote, 1998.1.20, Roote, 1995.5.4, Ashburner et al., 1990)

Name Synonyms

Secondary FlyBase IDs

References (22)

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