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General Information
Symbol
Dmel\lacek05305
Species
D. melanogaster
Name
FlyBase ID
FBal0011566
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
l(2)k05305
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P-element insertion 8-10 bp upstream of the lace transcription start site.

P{lacW} insertion 8 to 10bp upstream of the 5' end of the transcription unit.

Insertion components
P{lacW}lacek05305
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Unlike wild-type and lace[2] heterozygous controls, lacek05305/lace2 and lacek05305/laceU2 transheterozygous adults show a mushroom body "lost-lobe" phenotype in about half of cases; the overall dendrite organization in lacek05305/lace2 seems unaffected, as indicated by microtubule polarity (nod) and compartment identity (Apc2, DenMark, etc). Single cell analysis reveals a failure of lacek05305/lace2 mushroom body neurons to extend their axon branches dorsally, instead developing parallel horizontal branches. In lacek05305/lace2 larvae and pupae the vertical α/α' Kenyon cell lobe is already missing. Despite the missing lobe in larvae, the α/β axons and α'/β' axons still segregate into distinct horizontal lobes; there is non-segregation of the α' and β' growth cones, despite of unperturbed peduncle development; γ Kenyon cells do not seem to be affected.

lace2/lacek05305 and lacek05305/Df(3L)BSC671 larvae have significantly reduced bouton number and significantly increased bouton size at the neuromuscular junction of muscle 4, compared to controls, with no effect on the number of satellite boutons.

lacek05305/lace2 transheterozygous mutant adults present significantly decreased survival to starvation, decreased body weight, decreased adiposity in abdominal sections and decreased sizes of fat body cells and lipid droplets, but similar feeding behavior, as assessed by starvation-induced appetite, postprandial meal volume, average meal frequency and volume, and caloric intake, as compared to controls.

When raised on a standard diet, lacek05305 mutants survive to adulthood, although in reduced numbers. However, when these mutants are raised on lipid-depleted medium+cholesterol, 90% arrest as third instar larvae. This arrest is likely to be caused by the lack of dietary sphingolipid in lipid-depleted media. More than 80% of arrested lacek05305 mutant larvae remain viable for at least 10 days by which time their heterozygote siblings have pupariated. When raised on lipid-depleted medium with cholesterol, homozygous lacek05305 mutant larvae die 3 days earlier than their heterozygous siblings.

Heterozygotes have normal photoreceptor organisation.

lacek05305/lace2 adults have an average flight score of 1.41 +/- 0.063 compared to 1.62 +/- 0.14 in wild-type flies in a standard flight performance assay. The average number of dorsal longitudinal flight muscles per hemithorax is 5.94 +/- 0.030 in the mutant flies, compared to 6.00 +/- 0.00 in wild-type flies. Homozygotes show 91% lethality. Those adults that do survive have a lifespan of less than one week and have pronounced morphological defects; wings are notched and often fail to inflate, bristles are missing and the eyes are often rough with irregular ommatidia. Surviving adults have an average of 4.82 +/- 0.53 dorsal longitudinal flight muscles per hemithorax, compared to 6.00 +/- 0.00 in wild-type flies.

lacek05305/lace2 shows reduced viability. lacek05305/lace2 flies often show incision of the wing margin, and there is an ectopic crossvein. The hexagonal array of the ommatidia is disrupted, especially along the equatorial plane. The deep pseudopupil pattern is normal. In the notum, the microchaetae lack pigment and are occasionally missing, while the number of macrochaetae is variable. In the leg and antenna, bifurcation of the distal portion is rarely seen. The lethality and various adult mutant phenotypes of lacek05305/lace2 flies can be rescued by feeding with sphingosine. The adult mutant phenotypes cannot be rescued by exogenous sphingomyelin or ceramide. The wing, leg and eye-antennal discs of lacek05305/lace2 animals contain a considerably high number of dead cells. Severe malformation of the primordial wing blade is seen in the wing disc. The developing pupal eye shows loss of cells among cone cells, primary pigment cells, secondary pigment cells, tertiary pigment cells and interommatidial bristles.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Enhancer of
Suppressor of
Statement
Reference

lace[+]/lacek05305 is a suppressor of increased cell death phenotype of Sply05091

lace[+]/lacek05305 is a suppressor of lethal | recessive phenotype of Sply05091

lace[+]/lacek05305 is a suppressor | partially of flightless | recessive phenotype of Sply05091

lace[+]/lacek05305 is a suppressor of semi-sterile | recessive phenotype of Sply05091

NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
Suppressor of
Statement
Reference

lace[+]/lacek05305 is a suppressor of eye phenotype of DrefUAS.cSa

lace[+]/lacek05305 is a suppressor | partially of rhabdomere phenotype of Arr23

lace[+]/lacek05305 is a suppressor of rhabdomere phenotype of norpA39

Other
Additional Comments
Genetic Interactions
Statement
Reference

The mushroom body "lost lobe" phenotype penetrance observed in lacek05305/lace2 transheterozygous adults is significantly increased and decreased by the Scer\GAL4Dscam1.4.5-driven expression of Dscam1TM1.UAS.RFP(Unk) and Dscam1miRNA.UAS.17.1, respectively.

lacek05305/lace2 transheterozygous adults that also express Vap33UAS.cPa under the control of Scer\GAL4Tab2-201Y do not show a mushroom body "lost lobe" phenotype.

Introduction of heterozygous lacek05305 in the presence of Spt-IC129W.Scer\UAS and the pan-neuronal driver Scer\GAL4elav.PU induces a 50% reduction of bouton numbers compared with wild-type.

lacek05305/lace2, Sply05091/Sply05091 adults exhibit a more severe decrease in survival in response to starvation as compared to either lacek05305/lace2 or Sply05091/Sply05091 mutants.

The decreased body weight, decreased adiposity in abdominal sections and decreased sizes of fat body cells and lipid droplets, observed in lacek05305/lace2 transheterozygous mutant adults are not altered by Sply05091 homozygosity.

The decreased feeding behavior observed in Sply05091 homozygous mutant adults, as assessed by their decreased starvation-induced appetite, decreased postprandial meal volume, decreased average meal frequency and volume, and decreased daily food intake, is not suppressed by lacek05305/lace2 transheterozygosity.

One copy of lacek05305 partially suppresses the degeneration of rhabdomeres seen in Arr23 flies; the rhabdomeres are mostly intact in the rescued flies, but some cellular degeneration (characterised by vacuoles and dark multivesicular bodies) is still seen. The degeneration of photoreceptor cells seen in flies expressing shi1.Scer\UAS under the control of Scer\GAL4GMR.PF is substantially suppressed by lacek05305/+. One copy of lacek05305 suppresses the retinal degeneration seen in norpA39 flies.

Sply05091/Sply05091 lacek05305/+ adults have an average flight score of 1.41 +/- 0.063 compared to 2.60 +/- 0.032 in wild-type flies in a standard flight performance assay. The average number of dorsal longitudinal flight muscles per hemithorax is 5.13 +/- 0.26 in the mutant flies, compared to 6.00 +/- 0.00 in wild-type flies. Sply05091 lacek05305 double homozygotes show 61% lethality. The eye, bristle and wing defects of lacek05305 surviving adults are suppressed in Sply05091 lacek05305 double homozygotes.

rlSem suppresses the wing margin incision and loss of microchaetae phenotypes of lacek05305/lace2 flies.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

I. Kiss.

Comments
Comments

The phenotype is caused by the P{lacW} insertion.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (21)