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General Information
Symbol
Dmel\robo2lea-2
Species
D. melanogaster
Name
FlyBase ID
FBal0011613
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
lea2
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
robo2lea-2 homozygous embryonic heart cardioblasts show a rounded morphology, show significant decreases in migration velocity, as well as in filopodial and lamellopodial extensions and activities, as compared to controls.
learobo2-8/lea2 stage 13 embryos have gonads with unfused somatic gonadal precursor (SGP) clusters. By stage 15, SGP clusters fuse but gonads then fail to compact properly. Mutants also show germ cell ensheathment defects.
lea2 embryos show defective heart development. Heart defects include contralaterally placed cardial cell nuclei, missing cardial cells from rows of migrating cardial cells and a low level of delays in cell migration.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
NOT Enhancer of
Suppressor of
Statement
Reference
robo2[+]/robo2lea-2 is a suppressor of visible phenotype of DysdsRNA.C.UAS, Scer\GAL4Tub.PU
robo2[+]/robo2lea-2 is a suppressor of visible phenotype of DgdsRNA.UAS, Scer\GAL4Tub.PU
Other
Phenotype Manifest In
Suppressed by
NOT suppressed by
Enhancer of
Statement
Reference
lea[+]/robo2lea-2 is an enhancer of eye phenotype of Scer\GAL4GMR.PF, fruNP0021
lea[+]/robo2lea-2 is an enhancer of heart primordium phenotype of sli2
NOT Enhancer of
Suppressor of
Statement
Reference
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
robo11, robo2lea-2 double homozygous embryonic heart cardioblast show similar cell rounding, slow migration and decreased filopodial and lamellopodial activities) to those observed in robo11 single homozygotes; these defects are associated with gaps in the leading edge (and lost adhesions with ipsilateral partners), cell clumping, improper linear alignment of the cardioblasts, and heart lumen formation defects, as compared to controls. The decreased lamellopodial activity, but not the decreased filopodial activity, slow migration, or heart lumen formation, are only partially suppressed by the expression of robo1Scer\UAS.cKa under the control of Scer\GAL4Mef2.247.
A lea2 background enhances the rough eye phenotype related to fru isoform B, found upon expression of fruNP0021 under the control of Scer\GAL4GMR.PF.
One copy of robo2lea-2 weakly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.C.Scer\UAS is expressed under the control of Scer\GAL4tub.PU but produces extra wing vein material. One copy of robo2lea-2 suppresses the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.
lea2/+, sli2/+ embryos produce a mild heart development phenotype, with some rows of migrating cardial cells missing cells and slight delays in cardial cell migration. lea2/+, scb01288/+ embryos show a heart formation phenotype, including the appearance of contralaterally placed cardial cell nuclei.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (11)