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General Information
Symbol
Dmel\lt11
Species
D. melanogaster
Name
FlyBase ID
FBal0011687
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
lt40-12, EMS40-12
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

lt11/Df(2L)lt45 mutant larvae exhibit trafficking defects in the brain, fat body, salivary glands and garland cells. In the brain, the lysosomal marker Lamp mislocalizes to cytoplasmic granules. In the salivary gland, the lysosomal marker Lamp mislocalizes to vesicles instead of showing the normal localization to the Lysotracker-positive tubulo-vesicular lysosomal network, and the lysosomal network seems fragmented. In the fat body, the lysosomal marker Lamp shows some mislocalization to vesicles instead of showing the normal localization to large, Lysotracker-positive autolysosomes, which are much smaller than in controls. In the Garland cells there are enlarged vacuoles and postendocytic trafficking defects (e.g. endocytozed proteins fail to be efficiently incorporated into Lysotracker-positive autolysosomes), as compared to controls.

Autolysosome formation is inhibited in the fat body cells of starved lt11/Df(2L)lt45 mutant wandering third instar larvae. Large number of autophagosomes are observed. Numerous dense organelles are seen, which are usually round and smaller than the autolysosomes observed in controls. A subset of these vesicles contain recognisable remnants of cytoplasmic material, such as mitochondrion.

lthdS1A/lt11 flies have 60% wild-type viability.

Reduced eye and Malpighian tubule cell pigmentation, variable crumpled wings and missing bristles. Moderate position effect variegation is seen with lt rearrangements.

Hemizygotes die at late pupal stages.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

Su(var)2-1+ :, /lt1/lt11 has abnormal eye color phenotype, enhanceable by Su(var)2-1[+]/Su(var)2-1[+]/Su(var)2-11/Su(var)2-1v9

lt1/lt11 has abnormal eye color phenotype, enhanceable by Su(var)208[+]/Su(var)208unspecified/Su(var)205[+]/Su(var)2051

lt1/lt11 has abnormal eye color phenotype, enhanceable by Su(var)2-1[+]/Su(var)208[+]/Su(var)2-11/Su(var)208unspecified

lt1/lt11 has abnormal eye color phenotype, enhanceable by Su(var)2-1[+]/Su(var)208[+]/Su(var)208unspecified/Su(var)2-1v9

lt1/lt11 has abnormal eye color phenotype, enhanceable by Su(var)205[+]/E(Pc)[+]/Su(var)2051/E(Pc)unspecified

lt1/lt11 has abnormal eye color phenotype, enhanceable by Su(var)208[+]/Su(var)208unspecified/E(Pc)[+]/E(Pc)unspecified

Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference

Su(var)2-1+ :, /lt1/lt11 has pigment cell phenotype, enhanceable by Su(var)2-1[+]/Su(var)2-1[+]/Su(var)2-11/Su(var)2-1v9

lt1/lt11 has pigment cell phenotype, enhanceable by Su(var)208[+]/Su(var)208unspecified/Su(var)205[+]/Su(var)2051

lt1/lt11 has pigment cell phenotype, enhanceable by Su(var)2-1[+]/Su(var)208[+]/Su(var)2-11/Su(var)208unspecified

lt1/lt11 has pigment cell phenotype, enhanceable by Su(var)2-1[+]/Su(var)208[+]/Su(var)208unspecified/Su(var)2-1v9

lt1/lt11 has pigment cell phenotype, enhanceable by Su(var)2-11

lt1/lt11 has pigment cell phenotype, enhanceable by Su(var)2-1v9

lt1/lt11 has pigment cell phenotype, enhanceable by Su(var)205[+]/E(Pc)[+]/Su(var)2051/E(Pc)unspecified

lt1/lt11 has pigment cell phenotype, enhanceable by Su(var)208[+]/Su(var)208unspecified/E(Pc)[+]/E(Pc)unspecified

Other
Additional Comments
Genetic Interactions
Statement
Reference

Eye pigmentation in lt11/lt1 flies is reduced if the flies are also carrying E(Pc)unspecified/Su(var)2051, E(Pc)unspecified/Su(var)208unspecified, Su(var)208unspecified/Su(var)2051, Su(var)208unspecified/Su(var)2-11, Su(var)2-1v9/Su(var)2-11 or Su(var)2-1v9/Su(var)208unspecified. Eye pigmentation in lt11/+ flies that are also carrying E(Pc)unspecified/Su(var)208unspecified or Su(var)2-1v9/Su(var)208unspecified is reduced. Pigmentation is restored to wild-type levels in these flies by Dp(2;Y)G. XY lt11/lt1; Su(var)2-1v9/Su(var)2-11 males have reduced viability, which is restored in XO males. lt11/lt1; Su(var)2-1v9/Su(var)2051 flies have reduced viability. Viability is partially restored in XO males. lt11/lt1; Su(var)208unspecified/Su(var)2051 flies have reduced viability. Viability is restored in XO males. Eye pigmentation is reduced and viability is essentially wild-type in lt11/+ flies that are also carrying E(Pc)unspecified and E(ltv)319319, E(Pc)unspecified and E(ltv)335335 or Su(var)2-1v9 and E(ltv)319319. Eye pigmentation and viability is reduced in lt11/+ flies that are also carrying Su(var)208unspecified and E(ltv)319319 or Su(var)208unspecified and E(ltv)335335. Eye pigmentation is nearly wild-type and viability is reduced in lt11/+ flies that are also carrying Su(var)2-1v9 and E(ltv)335335.

Eye colour: lt1/lt11 g53d double mutants show a reduction in red eye pigment compared to either lt1/lt11 or g53d single mutants.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Not rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

Hilliker.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (10)