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General Information
Symbol
Dmel\Rab321
Species
D. melanogaster
Name
FlyBase ID
FBal0011737
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ltd1
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C9179184G

Reported nucleotide change:

C?G

Amino acid change:

Y484term | Rab32-PB; Y186term | Rab32-PC; Y20term | Rab32-PD; Y186term | Rab32-PE; Y12term | Rab32-PH; Y187term | Rab32-PI; Y12term | Rab32-PJ

Reported amino acid change:

??term

Comment:

Mutation is in the common exon of the predicted ltd transcripts and eliminates the C-terminal 203 amino acid region. Position of mutation on reference sequence inferred by FlyBase curator based on author statement.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Mutation is in the common exon of the predicted CG8024 transcripts and eliminates the C-terminal 203 amino acid region.

Nucleotide substitution: C?G.

Amino acid replacement: ??term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mutant flies show a significant decrease in ommochrome pigment levels and some reduction in drosopterin pigment levels compared to wild type.

Mutant flies have reduced red pigment in the eye compared to controls.

The primary pigment cells in ltd1 flies lack the large electron-lucent xanthommatin granules seen in wild type, while the granules in the secondary and tertiary pigment cells are fewer in number than normal and abnormal in shape and density of their content. The photoreceptor cells lack the small xanthommatin pigment granules seen in wild type.

Uptake of kynurenine, which is produced in the fat body, by the Malpighian tubes, where it is converted to 3-hydrokynurenine, is defective; similar defect seen in eye discs. Sullivan and Sullivan postulate that ltd is a transport mutant that prevents the substrate kynurenine from reaching the sites of its conversion to ommochrome.

Exhibits reduced levels of phenoazinone synthetase, an enzyme involved in the condensation of 3OH kynurenine molecules to xanthommatin, as do other mutants deficient in ommochrome synthesis.

Administration of 3 hydroxykynurenine without effect on eye colour.

Studied phenotypic interaction with other eye-color mutants.

Deficient in ommochrome synthesis. Lack detectable levels of 3OH kynurenine despite normal levels of kynurenine hydroxylase activity.

Eye colour: clear, light, translucent yellowish pink; resembles lt1 but is lighter, darkens with age. Ocellus colour: colourless. Malpighian tubule colour: colourless.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Enhancer of
Statement
Reference

Rab321 is an enhancer of eye color defective phenotype of Blos1ex2

Rab321 is an enhancer of visible phenotype of pp

Rab321 is an enhancer of eye color defective phenotype of pp

Rab321 is an enhancer of visible phenotype of psnb

Other
Phenotype Manifest In
NOT suppressed by
Statement
Reference

Rab321 has phenotype, non-suppressible by su(Hw)2

Enhancer of
Statement
Reference

Rab321 is an enhancer of pigment cell phenotype of psnb

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The reduction in ommochrome pigment levels that is seen in ltd1 flies is not affected by HPS4W515X.

Expression of auxScer\UAS.cHa under the control of Scer\GAL4GMR.PF enhances the loss of red eye pigment that is seen in ltd1 flies.

The reduction in red pigment in the eye seen in ltd1 blos1ex2 double mutants is more severe than that seen in either single mutant.

Eye colour: ltd1; psnb eyes are lighter and have significantly reduced levels of red pigment compared to either single mutant.

The level of pteridine eye pigments in ltd1 ; ca1 double mutant flies resembles that seen in ca1 single mutants. The level of pteridine eye pigments in g1 ; ltd1 or rb1 ; ltd1 double mutant flies are significantly lower than that of the corresponding single mutants.

Eye colour: nearly white, with rb1. Eye colour: nearly white, with g1. Pale eye color due to the absence of pteridines and brown pigment.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (2)
Stocks (5)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (15)