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General Information
Symbol
Dmel\lzts1
Species
D. melanogaster
Name
FlyBase ID
FBal0011835
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    point mutation
    Nucleotide change:

    A9288521T

    Reported nucleotide change:

    A11268T

    Amino acid change:

    N351I | lz-PA; N351I | lz-PB

    Reported amino acid change:

    N351I

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Amino acid replacement: N351I.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    lzts1 mutant females raised at 16[o]C and shifted to 29[o]C at third instar larval stage fail to form spermathecae and accessory glands. Mutants shifted to 29[o]C at 24 hours after pupal do show formation of spermathecal ducts, but at least one of the spermathecal caps is absent in these animals.

    lzts1 pupal retina that have been temperature-shifted (to the non-permissive temperature of 33oC at 20 hours after pupal formation) exhibit an increase in the number of 2o and 3o pigment cells compared to lzts1 flies at the permissive temperature (25oC) or controls (at 49 hours APF). On average, there are an additional two cells per ommatidium, which is similar to the number of cells that normally die. At 55 hours after pupal formation retina still exhibit additional 2o and 3o pigment cells, indicating that apoptosis is not delayed. There is a reduction in apoptosis in lzts1 retina, although perimeter cell death is not affected in lzts1 mutants. Temperature-shifted lzts1 adult eyes are only mildly rough and have wild-type pigmentation.

    lzts1 flies lack all basiconic sense organs.

    At 18oC, sections through the ommatidia appear normal in lzts1 mutants, while at 29oC defects in the lens and fenestrated membrane are seen. Ectopic cell death is seen in third larval instar eye discs at 29oC.

    The eye appears normal at 25oC in lzts1 mutants, while at 29oC, posterior eye defects are seen.

    Mutant flies have near wild-type eyes at 25oC, but have a rough eye phenotype at 29oC.

    lzts1/Y flies raised at 25oC have wild-type eyes.

    Crystal cells develop normally at 25oC but development is blocked at 29oC.

    The number of sense organ founder cells seen in the antennal disc 10 hours after pupariation is reduced compared to wild-type in animals pulsed at 29oC from the second larval instar stage onwards. The number of basiconic sensilla of the antenna is reduced compared to wild-type at 25oC. The number of trichoid sensilla and coeloconic sensilla is unaffected at this temperature. The number of trichoid sensilla is increased compared to wild-type at 28oC. The trichoid region of the antenna is largely unaffected, but several "ectopic" trichoid sensilla appear in the region occupied exclusively by basiconic sensilla in the wild-type.

    The amplitude of the physiological response of the antenna to ethyl acetate is reduced compared to wild-type, with the severity of the phenotype increasing as the temperature increases. This phenotype is at least partially rescued if the flies are also carrying Dp(1;Y)y+lz+.

    Homozygotes have normal eyes when raised at 18oC. When raised at 25oC, subtle eye defects are seen; bristles of the hair nerve groups grow abnormally long and may be misplaced, although lenses are generally well formed. At 29oC, flies have a more severe phenotype, with poorly formed, fused or missing lenses, abnormally long bristles that are misplaced and a general lack of ommatidial structure.

    Adults show missing, fused and reduced ommatidia and absence or duplication of several interommatidial bristles when raised at the restrictive temperature of 28oC. The eye phenotype overlaps wild-type at 25oC, the only defect being the incorrect location of some interommatidial bristles. The number of basiconic sensilla on the antenna is reduced by 30% at 25oC. Flies raised at 28oC show a striking reduction in the number of basiconic sensilla on the antenna, which is accompanied by a small but significant increase in the number of trichoid sensilla. The basiconic-like sensilla of the maxillary palps occasionally have abnormal shaft morphology, being composed of a short spine surrounded by a socket. The eye phenotype but not the antennal or maxillary palp sensilla phenotype of lzts1 flies is dominantly enhanced by E(lz)151, E(lz)531, E(lz)1741, E(lz)1781 and E(lz)2051.

    The antennal funiculus is reduced in size in hemizygous males raised at 28oC or 29oC. All large and small basiconic sensilla on the antennal funiculus are missing, and the number of trichoid sensilla on the antennal funiculus is reduced. The number of coeloconic sensilla on the antennal funiculus is normal. The density of the coeloconic sensilla on the antennal funiculus is increased and the density of the trichoid sensilla on the antennal funiculus is decreased compared to wild-type in hemizygous males raised at 29oC. The density of the coeloconic and trichoid sensilla on the antennal funiculus is increased compared to wild-type in hemizygous males raised at 28oC. The antennal funiculus is reduced in size in hemizygous males raised at 25oC or 27oC. The number of large and small basiconic sensilla on the antennal funiculus is greatly reduced, and the number of trichoid sensilla on the antennal funiculus is increased. The number of coeloconic sensilla on the antennal funiculus is normal. The density of the coeloconic and trichoid sensilla on the antennal funiculus is increased and the density of the basiconic sensilla on the antennal funiculus is decreased compared to wild-type. The antennal funiculus is reduced in size in hemizygous males raised at 18oC. The number of large and small basiconic sensilla is reduced. The density of the coeloconic and trichoid sensilla on the antennal funiculus is increased and the density of the small basiconic sensilla on the antennal funiculus is decreased compared to wild-type.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Enhanced by
    Statement
    Reference

    lzts1 has visible phenotype, enhanceable by Ras85DN17.hs.2sev

    lzts1 has visible phenotype, enhanceable by aopACT.sev

    lzts1 has visible phenotype, enhanceable by aopS2382/aop[+]

    lzts1 has visible phenotype, enhanceable by aopACT.GMR

    lzts1 has visible | heat sensitive phenotype, enhanceable by Bgb[+]/Bgb9

    lzts1 has visible | heat sensitive phenotype, enhanceable by BgbD/Bgb[+]

    lzts1 has visible | heat sensitive phenotype, enhanceable by BgbD/Df(3L)Bgb-K4

    lzts1 has visible | heat sensitive phenotype, enhanceable by +/Df(3L)Bgb-K4

    lzts1 has visible | heat sensitive phenotype, enhanceable by Hsp83[+]/Hsp833C

    lzts1 has visible | heat sensitive phenotype, enhanceable by Hsp834C/Hsp83[+]

    lzts1 has visible | heat sensitive phenotype, enhanceable by en(lz)Y[+]/en(lz)YY

    lzts1 has visible | heat sensitive phenotype, enhanceable by osa4F/osa[+]

    lzts1 has visible | heat sensitive phenotype, enhanceable by osa4H/osa[+]

    lzts1 has visible | heat sensitive phenotype, enhanceable by sv[+]/svI

    lzts1 has visible | heat sensitive phenotype, enhanceable by sv[+]/sv4G

    lzts1 has visible | recessive | heat sensitive phenotype, enhanceable by E(lz)531

    lzts1 has visible | recessive | heat sensitive phenotype, enhanceable by E(lz)1741

    lzts1 has visible | recessive | heat sensitive phenotype, enhanceable by E(lz)1781

    lzts1 has visible | recessive | heat sensitive phenotype, enhanceable by E(lz)2051

    lzts1 has visible | recessive | heat sensitive phenotype, enhanceable by E(lz)151

    Suppressed by
    Statement
    Reference

    lzts1 has visible phenotype, suppressible | partially by Ras85DV12.sev

    Other
    Statement
    Reference
    Phenotype Manifest In
    Enhanced by
    Statement
    Reference

    lzts1 has eye phenotype, enhanceable by Ras85DN17.hs.2sev

    lzts1 has eye phenotype, enhanceable by aopACT.sev

    lzts1 has eye phenotype, enhanceable by aopS2382/aop[+]

    lzts1 has eye phenotype, enhanceable by aopACT.GMR

    lzts1 has eye phenotype, enhanceable by Bgb[+]/Bgb9

    lzts1 has eye phenotype, enhanceable by BgbD/Bgb[+]

    lzts1 has eye phenotype, enhanceable by BgbD/Df(3L)Bgb-K4

    lzts1 has eye phenotype, enhanceable by +/Df(3L)Bgb-K4

    lzts1 has eye phenotype, enhanceable by Hsp83[+]/Hsp833C

    lzts1 has eye phenotype, enhanceable by Hsp834C/Hsp83[+]

    lzts1 has eye phenotype, enhanceable by en(lz)Y[+]/en(lz)YY

    lzts1 has eye phenotype, enhanceable by osa4F/osa[+]

    lzts1 has eye phenotype, enhanceable by osa4H/osa[+]

    lzts1 has eye phenotype, enhanceable by sv[+]/svI

    lzts1 has eye phenotype, enhanceable by sv[+]/sv4G

    lzts1 has phenotype, enhanceable by svspa-pol/sv[+]

    lzts1 has eye phenotype, enhanceable by E(lz)531

    lzts1 has eye phenotype, enhanceable by E(lz)1741

    lzts1 has eye phenotype, enhanceable by E(lz)1781

    lzts1 has eye phenotype, enhanceable by E(lz)2051

    lzts1 has eye phenotype, enhanceable by E(lz)151

    Suppressed by
    Statement
    Reference

    lzts1 has eye phenotype, suppressible | partially by Ras85DV12.sev

    Suppressor of
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    Reducing lz activity by transferring lzts1 WGMR.PG flies to the non-permissive temperature of 33oC partially suppresses the WGMR.PG eye phenotype. Reducing lz activity by transferring lzts1 rprGMR.PW flies to the non-permissive temperature of 33oC partially suppresses the rprGMR.PW eye phenotype. Expression of argosScer\UAS.cHa, under the control of Scer\GAL4hs.2sev in temperature-shifted lzts1 mutants results in a few small clusters of 2o/3o cells, which are not seen in argosScer\UAS.cHa Scer\GAL4hs.2sev single mutants. Reducing Egfr signaling (using temperature-sensitive Egfrtsla) in lzts1 retinas results in an increase in cell death.

    lzts1 ; aopACT.GMR flies have a markedly reduced eye at 29oC, with the loss of all ommatidial structures. aopS2382/+ enhances the lzts1 phenotype at 29oC, with defects extending throughout the eye. Ras85DN17.hs.2sev enhances the lzts1 eye phenotype, making the eye rough at the permissive temperature for lzts1 and severely defective at 29oC. Ras85DV12.sev suppresses the posterior flattening of the eye seen in lzts1 flies at 29oC.

    Rare lzts1 ; BgbD/Df(3L)Bgb-K4 escapers are seen. These flies are extremely weak and can only be rescued if they are dissected from the pupal case. They have a strong adult eye phenotype that is identical to that of a null lz mutant.

    svspa-pol dominantly enhances the lzts1 phenotype.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    Comments
    Comments

    Temperature shift experiments indicate that lz+ function is crucial from approximately 87% of the third larval instar stage up to 7% of pupal life.

    lzts1 maps to the spectacle sublocus of lz.

    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (4)
    Reported As
    Name Synonyms
    Secondary FlyBase IDs
      References (16)