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General Information
Symbol
Dmel\m1
Species
D. melanogaster
Name
FlyBase ID
FBal0011837
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
m
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
complex substitution
Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement: Deletion of 33 nucleotides associated with an insertion of 13 nucleotides, 370 amino acids downstream from the start of translation. Leads to premature stop codon.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Deletion of 33 nucleotides associated with an insertion of 13 nucleotides, 370 amino acids downstream from the start of translation. This causes a frameshift mutation and premature stop codon within the coding region.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mutant larvae have thick, broad denticles.

Each denticle row is present, but the denticles themselves are small and lack the median constriction, adopting an abnormal triangular shape.

Mutants show a strong reduction in the size of adult wings.

m1 embryos have impaired contacts between the denticle membrane and cuticle layers

m1 larvae have small, misshapen denticles that display an aberrant triangular shape. Additionally, the denticle cells do not contact cuticle correctly.

m68/m1 females have a m mutant phenotype and are fertile.

m1/In(1)mK females may or may not show the m mutant phenotype, some being normal or almost wild-type.

Wing size reduced, only slightly longer than abdomen and with normal proportions. Angle between L2 and L5 reduced. Wings dark gray and less transparent than normal. Wing cells smaller than normal (Dobzhansky, 1929). In poor cultures, wings may become divergent and stringy. Cell expansion inhibited in prepupae and pupae [Waddington, 1940). RK1.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhancer of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference

m1 has denticle phenotype, enhanceable by sn3

m1 has denticle phenotype, enhanceable by f36a

f36a, m1 has denticle phenotype, enhanceable by sn3

m1, sn3 has denticle phenotype, enhanceable by f36a

f36a, m1, sn3 has denticle phenotype, enhanceable by shaV15

f36a, m1, sn3 has denticle phenotype, enhanceable by WASp3

Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

m1 has denticle phenotype, non-suppressible by nyoUAS.cFa/Scer\GAL4da.G32

m1 has wing phenotype, non-suppressible by nyoUAS.cFa/Scer\GAL4da.G32

m1 has denticle phenotype, non-suppressible by tynΔPAN.UAS/Scer\GAL4da.G32

m1 has wing phenotype, non-suppressible by tynΔPAN.UAS/Scer\GAL4da.G32

m1 has denticle phenotype, non-suppressible by Scer\GAL4da.G32/tynUAS.cFa

m1 has wing phenotype, non-suppressible by Scer\GAL4da.G32/tynUAS.cFa

m1 has wing phenotype, non-suppressible by dylUAS.cFa/Scer\GAL4da.G32

m1 has denticle phenotype, non-suppressible by zyeUAS.cFa/Scer\GAL4da.G32

m1 has wing phenotype, non-suppressible by zyeUAS.cFa/Scer\GAL4da.G32

m1 has denticle phenotype, non-suppressible by neoUAS.cFa/Scer\GAL4da.G32

m1 has wing phenotype, non-suppressible by neoUAS.cFa/Scer\GAL4da.G32

m1 has phenotype, non-suppressible by su(Hw)2

Enhancer of
Statement
Reference

m1 is an enhancer of denticle phenotype of sn3

m1 is an enhancer of denticle phenotype of f36a

NOT Enhancer of
Statement
Reference
NOT Suppressor of
Statement
Reference

m1 is a non-suppressor of eye phenotype of Ppt1UAS.cKa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF

Other
Additional Comments
Genetic Interactions
Statement
Reference

m1 ; ckKT9 double mutants show a reduction in both size and sharpness of the denticles compared to either single mutant.

m1 f1 ckKT9 triple mutants show almost no elongation of the denticles.

m1, tynPG38 double mutants have denticles with thin extremities (as found in tynPG38 single mutants) and lacking the medial constriction (as found in m1 single mutants). The addition of Df(3L)zyeex72, which deletes zye, has a further effect, leading to denticles with a twisted appearence.

Expression of dylScer\UAS.cFa with Scer\GAL4da.G32 slightly modifies the m1 denticle phenotype, but doesn't rescue m1 the adult wing phenotype.

Expression of m::tynPAN-tyn.Scer\UAS with Scer\GAL4da.G32 rescues the m1 denticle phenotype and the m1 adult wing phenotype.

Expression of m::tynZPtyn.Scer\UAS with Scer\GAL4da.G32 fails to rescue the m1 denticle phenotype and only partially restores the m1 adult wing phenotype.

Expression of m::tynZPm.Scer\UAS with Scer\GAL4da.G32 has a weak effect on the m1 denticle phenotype and significantly rescues the m1 adult wing phenotype.

The denticles of m1, sn3 mutants show splitting in addition to the defects seen in either single mutant. m1, f36a mutants also show denticle splitting. sn3, m1, f36a triple mutants have small, highly misshapen denticles that are more affected than those of m1, f36a or m1, sn3 double mutants. sn3, m1, f36a; shaV15 and sn3, m1, f36a; WASp3 quadruple mutants show further impairment to denticle formation compared to sn3, m1, f36a triple mutants, with mutants exhibiting regions of naked cuticle where denticles lie in wild-type animals.

The dorsal hairs on the abdominal segments of sn3, m1, f36a triple mutants are severly reduced in size, and in some cases, hairs are abrogated leaving abnormal naked regions. This phenotype is more severe in sn3, m1, f36a; WASp3 quadruple mutants and is even more severe in sn3, m1, f36a; shaV15 mutants in which most dorsal hairs are absent, leaving naked cuticle.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Not rescued by
Comments

Expression of mScer\UAS.cFa or mScer\UAS.T:Arus\HRP using Scer\GAL4da.G32 rescues the denticle and adult wing size phenotypes of m1 animals.

Expression of mScer\UAS.cFa using Scer\GAL4da.G32 rescues the denticle defects of m1 embryos and the reduction in wing size of m1 adults.

Images (1)
Stocks (31)
Notes on Origin
Discoverer

Morgan, Aug. 1910.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (16)