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General Information
Symbol
Dmel\RpL27A1
Species
D. melanogaster
Name
FlyBase ID
FBal0011887
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
M(2)z, M
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Heterozygous RpL27A1 mutant females exhibit a dilated cardiomyopathy phenotype, including an increased end-systolic dimension (EDD) and a decreased fractional shortening (FS) compared to controls. Bristles appear short and thin compared to wild type (Minute phenotype).

Mutants show a significant developmental delay and somewhat smaller larval body sizes than normal, but no obvious morphological neuromuscular junction phenotypes.

Heterozygotes have a Minute bristle phenotype and appreciably larger and rougher eyes than normal. Larval development of heterozygotes is delayed by about 1.2 days at 25oC compared to control siblings.

RK2.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Suppressor of
Statement
Reference
Other
Phenotype Manifest In
NOT Enhanced by
Statement
Reference

RpL27A1 has phenotype, non-enhanceable by M(2)31A1

RpL27A1 has phenotype, non-enhanceable by M(2)281

RpL27A1 has phenotype, non-enhanceable by M(3)69E1

Suppressed by
NOT suppressed by
Statement
Reference

RpL27A1 has phenotype, non-suppressible by M(2)31A1

RpL27A1 has phenotype, non-suppressible by M(2)281

RpL27A1 has phenotype, non-suppressible by M(3)69E1

Enhancer of
Statement
Reference

RpL27A1/M(2)24F[+] is an enhancer of phenotype of J1

NOT Enhancer of
Statement
Reference

RpL27A1 is a non-enhancer of phenotype of M(2)31A1

RpL27A1 is a non-enhancer of phenotype of M(2)281

RpL27A1 is a non-enhancer of phenotype of M(3)69E1

Suppressor of
NOT Suppressor of
Statement
Reference

RpL27A1 is a non-suppressor of phenotype of M(2)31A1

RpL27A1 is a non-suppressor of phenotype of M(2)281

RpL27A1 is a non-suppressor of phenotype of M(3)69E1

Other
Statement
Reference

Dl[+]/Dl1, RpL27A1 has leg phenotype

Dl1, RpL27A1 has leg phenotype

Dl1, RpL27A1 has wing phenotype

Dl1, RpL27A1 has femur phenotype

Dl[+]/Dl1, RpL27A1 has wing phenotype

Dl[+]/Dl1, RpL27A1 has wing vein phenotype

Dl[+]/Dl1, RpL27A1 has tarsal segment phenotype

Dl[+]/Dl1, RpL27A1 has femur phenotype

Dl[+]/Dl1, RpL27A1 has ocellus phenotype

Dl[+]/Dl1, RpL27A1 has macrochaeta phenotype

Additional Comments
Genetic Interactions
Statement
Reference

Larvae expressing l(2)glGD4047 under the control of Scer\GAL4en.PU in a minute background (RpL27A1) live for about 11 days after egg laying and die with no signs of differentiation. The cells of the P-compartment of the wing disc appear highly disorganised due to loss of apical-basal polarity and increased hypoxia is seen compared to controls. At the extreme end of the larval stage these tumor cells are highly migratory, migrating towards the tracheal tubes and contacting their filopodia. The tumor cells also form new tracheal branches and can migrate along tracheal vessels and fuse with them. Tumor cells are seen to migrate from one disc to another, for example from the haltere disc, along a tracheal scaffold, to the wing disc.

Expression of bskDN.Scer\UAS suppresses the tumor growth seen when l(2)glGD4047 is expressed under the control of Scer\GAL4en.PU in a minute background (RpL27A1). Individuals differentiate into adult pharates and a small proportion of the pupae eclose into adults with no evident phenotypic alterations.

Dl1 M(2)24F1 double heterozygotes do not eclose, although many flies form pharate adults. These flies have many abnormalities, including shortening of the legs. The tarsus, which is primarily affected, is sometimes reduced to half the size of the tibia. The femur is bent like a bow. One pair of legs (usually the last pair) may be more affected than the others. The wings are often scalloped and have an extreme Dl mutant phenotype; showing both thickening at the crossveins and deltas at the wing margin. The texture of the wing is very brittle. The eye are very rough or may be reduced in size. The ocelli are sometimes fused or one of them may be missing. The bristles are larger than seen in the M(2)24F1 single heterozygote and are often duplicated. Irregular additional hairs are seen on the thorax, as in Dl1 single heterozygotes.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (4)
Notes on Origin
Discoverer

Schultz.

Comments
Comments
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
References (14)