FlyBase Allele Report: Dmel\mei-41[D5]

General Information

Symbol

Dmel\mei-41^D5

Species

D. melanogaster

Name

FlyBase ID

FBal0012156

Feature type

allele

Associated gene

Dmel\mei-41

Associated Insertion(s)

Carried in Construct

Also Known As

mei-41D5, mei41^D5

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: P2159L.

(Laurencon et al., 2003)

In addition to the P2159L amino acid replacement, which may cause the mutant phenotype, a number of additional amino acid changes are present compared to the U34925 mei-41 GenBank sequence (these "common" mutations are also present in other mei-41 mutant alleles, possibly due to variability present in the mutagenised population).

(Laurencon et al., 2003)

Nucleotide substitution: C8924T.

(Laurencon et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:16,398,915..16,398,915 [+]

Nucleotide change:

C16398915T

Reported nucleotide change:

C8924T

Amino acid change:

P2322L | mei-41-PA

Reported amino acid change:

P2159L

Comment:

The amino acid change is reported relative to GB:U34925 (in which the predicted CDS is missing 163 N-terminal amino acids relative to genome release 3.2). Other amino acid changes common to several mei-41 mutants are also present in the strain.

(Laurencon et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal DNA repair

(Ferro and Eeken, 1993, Eeken et al., 1985, Brown and Boyd, 1981)

abnormal DNA repair | oogenesis

(Laurencon et al., 2003)

abnormal meiotic cell cycle

chemical sensitive

(Laurencon et al., 2003, Olsen and Green, 1982)

chemical sensitive | recessive

(Flores and Engels, 1999)

female fertile

(Ferro and Eeken, 1993)

female semi-fertile

female semi-sterile

(Laurencon et al., 2003)

lethal - all die during embryonic stage | partially (with mei-41^RT1)

(Rickmyre et al., 2007)

lethal - all die during embryonic stage | recessive

(Sibon et al., 1999)

radiation resistant

(Zhou and Steller, 2003)

radiation sensitive

(Oliveri et al., 1990)

radiation sensitive | recessive

(Flores and Engels, 1999, Kasravi et al., 1999)

some die during embryonic stage | partially (with mei-41^RT1)

(Rickmyre et al., 2007)

some die during embryonic stage | recessive

(Sibon et al., 1999)

viable

(Laurencon et al., 2003)

Phenotype Manifest In

Detailed Description

Statement

Reference

50% of eggs derived from homozygous females hatch into larvae. Hemizygous larvae show 74% loss of the G₂/M checkpoint (this number is the average number of mitotic cells per eye disc after exposure of male hemizygous larvae to 500R of X rays expressed as a percentage of the number of mitotic cells per eye disc before irradiation, wild-type values range from 5 to 15%).

(Laurencon et al., 2003)

Unlike wild-type, UVC irradiation of stage 10 mei-41^D5 mutant embryos at 50 mJ/cm² does not induce apoptosis.

(Zhou and Steller, 2003)

Mutants show an altered frequency of induced dominant lethal mutations in eggs after irradiation.

(Sarantseva and Khromykh, 2001)

Homozygotes are sensitive to methyl methanesulfonate and γ rays.

(Flores and Engels, 1999)

100% of homozygotes are killed by 60Gy radiation.

(Kasravi et al., 1999)

Approximately 4% of embryos derived from mei-41^D3/mei-41^D5 females hatch at 25^oC, while approximately 45% hatch at 20^oC.

(Sibon et al., 1999)

Excision repair capacity is normal and post-replication repair capacity is deficient.

(Ferro and Eeken, 1993)

Survivors of treatment with nitrogen mustard have slightly reduced flight ability, though the reduction is less than for mus308 mutants.

(Leonhardt et al., 1993)

Flies are sensitive to X rays.

(Oliveri et al., 1990)

Post replication repair deficient.

(Eeken et al., 1985)

The presence or absence of mei-41^D5 has little effect on the reversion rate of RpII215⁹.

(Voelker et al., 1984)

Reduces meiotic exchange.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

mei-41^D5/mei-41^RT1 has lethal | embryonic stage phenotype, enhanceable by MCPH1^Z1861/MCPH1[+]

(Rickmyre et al., 2007)

mei-41^D5/mei-41^D3 has lethal | embryonic stage | maternal effect phenotype, enhanceable by grp[+]/grp^fsA4

(Sibon et al., 1999)

Suppressed by

Statement

Reference

mei-41^D5/mei-41^D3 has lethal | maternal effect | embryonic stage phenotype, suppressible by CycA^unspecified/CycB^unspecified

(Sibon et al., 1999)

Other

Statement

Reference

CycA^unspecified, mei-41^D5 has lethal | recessive | maternal effect | embryonic stage phenotype

(Sibon et al., 1999)

CycB^unspecified, mei-41^D5 has lethal | recessive | maternal effect | embryonic stage phenotype

(Sibon et al., 1999)

CycA^unspecified, CycB^unspecified, mei-41^D5/mei-41^D3 has lethal | maternal effect | embryonic stage phenotype

(Sibon et al., 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

mei-41^D5 has phenotype, suppressible by rad201¹

(Sarantseva and Khromykh, 2001)

Additional Comments

Genetic Interactions

Statement

Reference

MCPH1^Z1861 dominantly enhances the embryonic lethality caused by mei-41^RT1/mei-41^D5.

(Rickmyre et al., 2007)

If mei-41^D3/mei-41^D5 females are also heterozygous for grp^fsA4, the hatch rates are reduced to 1% and 23% at 25^oC and 20^oC respectively. The lethality of embryos derived from mei-41^D3/mei-41^D5 females is partly rescued if the females are also heterozygous for either CycA^unspecified or CycB^unspecified, and greater rescue is seen if the females are also heterozygous for both CycA^unspecified and CycB^unspecified.

(Sibon et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (12)

Bloomington

4236

mei-41^D5 f¹; sv^spa-pol

5671

y¹ mei-9^a mei-41^D5 wap⁹/FM7a

5672

y¹ mei-9^a rtv¹⁰ mei-41^D5/FM7c

5650

y¹ l(1)2Fd¹⁹ mei-9^a mei-41^D5/FM7c

5669

y¹ mei-9^a mei-41^D5 l(1)19Ed¹⁷/FM7c

5401

y¹ mei-9^a mei-41^D5 l(1)ESHS46²/FM7c

Kyoto

107648

w^-; mei-41^D5/Basc; cn¹ bw¹

108352

y¹ mei-9^a l(1)ESHS27¹ mei-41^D5/FM7c

108353

y¹ mei-9^a l(1)ESHS29¹ mei-41^D5/FM7c

108351

y¹ mei-9^a l(1)ESHS26¹ mei-41^D5 / FM7c

109611

sc^* z¹ w^+(TE) mei-9^a mei-41^D5 / FM7c

101242

y¹ mei-9^a mei-41^D5 / FM7c, y^31d sc⁸ w^a sn^X2 v^Of g⁴ Bar¹

Notes on Origin

Discoverer

Comments

Homozygotes have normal excision repair capabilities, but postreplication repair is reduced compared to wild-type. Homozygous cultured cells respond to UV irradiation with normal reductions in thymidine incorporation and with the synthesis of abnormally short nascent DNA.

(Brown and Boyd, 1981)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

(Johnson-Schlitz et al., 2007)

mei-41^D5

(Jansen et al., 2024, Zheng et al., 2016, Kurzhals et al., 2011, Rickmyre et al., 2007, Sakaguchi and Steward, 2007, Gorski et al., 2004)

mei-41D5

(Khromykh and Kotlovanova, 2001, Sarantseva and Khromykh, 2001, Sarantseva and Khromykh, 2001, Kawai, 1997)

mei-41D⁵

(Khromykh and Sarantseva, 1998)

mei41^D5

(Kotlovanova et al., 2008, Flores and Engels, 1999)

mei41D⁵

(Sarantseva and Khromykh, 1999)

Name Synonyms

Secondary FlyBase IDs

References (49)

Report Sections

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