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General Information
Symbol
Dmel\mid2
Species
D. melanogaster
Name
FlyBase ID
FBal0012262
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:
C5466050T
Reported nucleotide change:
C?T
Amino acid change:
Q361term | mid-PA; Q361term | mid-PB
Reported amino acid change:
Q361term
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Nucleotide substitution: C?T.
Amino acid replacement: Q361term.
Nucleotide substitution: C1758T. (Nucleotide numbering is according to cDNA clone RE27439).
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
midB23/mid2 mutant embryos exhibit gonad development defects that are similar in penetrance and severity to midB23 homozygotes.
An additional, ectopic eve-postive neuron is observed within the nerve cord in 29-30% of hemisegments in ~14 hour-old mutant embryos. In midlos1/mid2 embryos, the ectopic neuron is seen in 24-25% of hemisegments; in mid2/mid1 embryos, the ectopic neuron is seen in 33-34% of hemisegments; in mid2/Df(2L)Exel6012 embryos, the ectopic neuron is seen in 50-56% of hemisegments. Further experiments suggest this is an ectopic RP2 (eRP2) neuron, resulting from the transformation of a wild type neuron termed the 'M-neuron', with an ectopic GMC-1-like cell and a RP2 sib-like cell also present. This extra RP2/sib lineage is formed 2-2.5 hours after the development of the bona fide RP2 lineage.
Mutant embryos show motorneuron projection defects.
Stage 16 mid2/Df(2L)cl-h2 embryos have a relatively normal number of cells in the dorsal vessel, but they have increased numbers of elongated, putative ostia forming cells per hemisegment in the heart region and a corresponding decrease in the number of cells expressing cardioblast markers.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (12)