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General Information
Symbol
Dmel\msl-31
Species
D. melanogaster
Name
FlyBase ID
FBal0012357
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
msl31, msl-3p, mle(3)132
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

One copy of msl-31 has no effect on hemocyte proliferation in third instar larvae.

One copy of msl-31 is unable to suppress position effect variegation (PEV) at the w locus caused by In(1)wm4.

One copy of msl-31 weakly suppresses the telomeric position effect (TPE) in stocks carrying a variegating P{hsp26-pt-T}39C-5 insertion at the telomere of the left arm of chromosome two.

Homozygous mutant msl-31 flies carrying msl-3ΔCD.T:Zzzz\TAP display a 2-day developmental delay with respect to wild-type controls and exhibit held-out wings. Adult males are unhealthy and infertile.

Homozygous mutant msl-31 flies carrying msl-3SYD62A.T:Zzzz\TAP display a 2-day developmental delay with respect to wild-type controls.

Homozygous mutant msl-31 flies carrying msl-3W59G.T:Zzzz\TAP display a 2-day developmental delay with respect to wild-type controls.

Homozygous males show appreciable survival to larval stages (day 5) in the presence of infection with the "male-killing" bacterium S.poulsonii when their mother is also homozygous, while infected heterozygous sibling males show no survival to day 5. Homozygous and heterozygous infected male embryos derived from heterozygous females show no survival to day 5.

Males survive until late larvae or early pupae, though their development is greatly delayed. The mle product is not associated with the X chromosomes in msl-3 mutants. mle binding to X chromosomes of Sxlf1/Sxlfhv1 females is also dependent on msl-3 function. Sxlf1/Sxlfhv1 females that are also homozygous for mutants at msl-1, msl-2 or msl-3 develop as intersexes of the mosaic type.

msl-31/msl-33 males show abnormal dosage compensation as determined by tritiated uridine labeling of polytene chromosomes.

Sxlf1 heterozygous female progeny derived from heterozygous da1 mothers have reduced viability. The viability is increased if the Sxlf1/+ progeny are also carrying msl-31, and is decreased if the da1/+ mothers are also carrying msl-31. The frequency of sex transformation in Sxlf1/+ female progeny derived from da1/+ mothers is increased if either the mothers or the progeny are homozygous for msl-31. The frequency of morphological abnormalities seen in Sxlf1/+ female progeny derived from da1/+ mothers is greatly reduced if the progeny are also homozygous for msl-31.

male-lethal

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

msl-3[+]/msl-31, scsisB-1, sisA[+]/sisA1 has lethal | dominant | female | maternal effect phenotype, suppressible | maternal effect | partially by SxlM1/Sxl[+]

Enhancer of
Statement
Reference
Suppressor of
Other
Statement
Reference
Phenotype Manifest In
NOT Enhancer of
Statement
Reference

msl-31 is a non-enhancer of phenotype of msl-1C.hs.Tag:FLAG

msl-31 is a non-enhancer of phenotype of msl-1Δ84.Tag:FLAG

Suppressor of
NOT Suppressor of
Statement
Reference

msl-31 is a non-suppressor of phenotype of msl-1C.hs.Tag:FLAG

msl-31 is a non-suppressor of phenotype of msl-1Δ84.Tag:FLAG

msl-31 is a non-suppressor of germline cell phenotype of ovounspecified

Other
Additional Comments
Genetic Interactions
Statement
Reference

Removing one copy of Rbp9P2690 during oogenesis in combination with zygotic heterozygosity for SxlfP7B0 results in female lethality. No female lethality is seen in the reverse cross. This female lethality is suppressed when msl-31 is also zygotically removed.

The viability of SxlfP7B0/+, sisA1/+ or Sxlf9/+ females is significantly reduced if they also carry one maternally-derived copy of msl-31.

The viability of scsisB-1/+ females raised at the non-permissive temperature of 29[o]C is significantly reduced if they also carry one maternally-derived copy of msl-31.

One maternally-derived copy of msl-31 significantly reduces the viability of scsisB-1 sisA1/+ females so that they show almost complete lethality. One paternally-derived copy of msl-31 significantly reduces the viability of scsisB-1 sisA1/+ females, although this zygotic effect is generally weaker than the maternal one.

The addition of msl-31 to flies containing a single copy of msl-1Δ84.T:Zzzz\FLAG in weak heatshock conditions has no effect on the male viability phenotype seen in these flies. The addition of msl-31 to flies containing a single copy of msl-1C.hs.T:Zzzz\FLAG in weak heatshock conditions has no effect on the male viability phenotype seen in these flies.

Does not suppress the germline death phenotype of ovounspecified females.

Sxlf1/+ chromosomal females also homozygous or heterozygous msl-31 show variable transformation of sexually dimorphic structures to male characteristics. The frequency of transformation is not high, is higher in females homozygous (rather than heterozygous) for msl-31, and expression is incomplete. The frequency of transformation is higher in female flies with mothers homozygous msl-31. Sex combs are only seen in Sxlf1/+ females homozygous for msl-31.

Xenogenetic Interactions
Statement
Reference

One copy of msl-31 moderately suppresses the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1Scer\UAS.cSa is expressed under the control of Scer\GAL4Hml.Δ.

Complementation and Rescue Data
Partially rescued by
Not rescued by
Comments

msl-3WT.T:Zzzz\TAP rescues the male lethality caused by msl-31.

msl-3Y31A.T:Zzzz\TAP partially rescues (31.9%) the male lethality caused by msl-31.

msl-3EPAA.T:Zzzz\TAP rescues the male lethality caused by msl-31.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

H4Ac16, acetylated form of His4, is not localised to the X chromosome in male salivary gland nuclei.

A P-element maps near the 5' end of the adjacent gene, anon-65Ea but may not be responsible for the mutant phenotype: rescue constructs that do not include the site of insertion of the P-element can fully rescue the msl-3 mutant phenotype.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
References (27)