FlyBase Allele Report: Dmel\msl-3[1]

General Information

Symbol

Dmel\msl-3¹

Species

D. melanogaster

Name

FlyBase ID

FBal0012357

Feature type

allele

Associated gene

Dmel\msl-3

Associated Insertion(s)

Carried in Construct

Also Known As

msl3¹, msl-3^p, mle(3)132, msl3^P

Key Links

Allele class

Mutagen

spontaneous

Nature of the Allele

Allele class

Mutagen

spontaneous

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates acute myeloid leukemia

modeled by Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa

(Sinenko et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with msl-3^{SYD62A.Tag:TAP}

(Sural et al., 2008)

abnormal developmental rate, with msl-3^W59G.Tag:TAP

(Sural et al., 2008)

abnormal developmental rate, with msl-3^ΔCD.Tag:TAP

(Sural et al., 2008)

abnormal developmental rate (with msl-3^{SYD62A.Tag:TAP})

(Sural et al., 2008)

abnormal developmental rate (with msl-3^ΔCD.Tag:TAP)

(Sural et al., 2008)

lethal

(Sural et al., 2008)

lethal | male

(Moore et al., 2010, Gorman et al., 1993, Uenoyama et al., 1982)

lethal | male | recessive

(Okuno et al., 1984)

lethal - all die before end of larval stage | male

(Bone et al., 1994)

male sterile, with msl-3^ΔCD.Tag:TAP

(Sural et al., 2008)

male sterile (with msl-3^ΔCD.Tag:TAP)

(Sural et al., 2008)

non-suppressor of variegation | dominant, with In(1)w^m4

(Doheny et al., 2008)

some die during larval stage | male

(Bone et al., 1994)

suppressor of variegation | dominant, with P{hsp26-pt-T}39C-5

(Doheny et al., 2008)

Phenotype Manifest In

wing, with msl-3^ΔCD.Tag:TAP

(Sural et al., 2008)

wing (with msl-3^ΔCD.Tag:TAP)

(Sural et al., 2008)

Detailed Description

Statement

Reference

One copy of msl-3¹ has no effect on hemocyte proliferation in third instar larvae.

(Sinenko et al., 2010)

One copy of msl-3¹ is unable to suppress position effect variegation (PEV) at the w locus caused by In(1)w^m4.

One copy of msl-3¹ weakly suppresses the telomeric position effect (TPE) in stocks carrying a variegating P{hsp26-pt-T}39C-5 insertion at the telomere of the left arm of chromosome two.

(Doheny et al., 2008)

Homozygous mutant msl-3¹ flies carrying msl-3^{ΔCD.T:Zzzz\TAP} display a 2-day developmental delay with respect to wild-type controls and exhibit held-out wings. Adult males are unhealthy and infertile.

Homozygous mutant msl-3¹ flies carrying msl-3^{SYD62A.T:Zzzz\TAP} display a 2-day developmental delay with respect to wild-type controls.

Homozygous mutant msl-3¹ flies carrying msl-3^{W59G.T:Zzzz\TAP} display a 2-day developmental delay with respect to wild-type controls.

(Sural et al., 2008)

Homozygous males show appreciable survival to larval stages (day 5) in the presence of infection with the "male-killing" bacterium S.poulsonii when their mother is also homozygous, while infected heterozygous sibling males show no survival to day 5. Homozygous and heterozygous infected male embryos derived from heterozygous females show no survival to day 5.

(Veneti et al., 2005)

Males survive until late larvae or early pupae, though their development is greatly delayed. The mle product is not associated with the X chromosomes in msl-3 mutants. mle binding to X chromosomes of Sxl^f1/Sxl^fhv1 females is also dependent on msl-3 function. Sxl^f1/Sxl^fhv1 females that are also homozygous for mutants at msl-1, msl-2 or msl-3 develop as intersexes of the mosaic type.

(Gorman et al., 1993)

msl-3¹/msl-3³ males show abnormal dosage compensation as determined by tritiated uridine labeling of polytene chromosomes.

(Okuno et al., 1984)

Sxl^f1 heterozygous female progeny derived from heterozygous da¹ mothers have reduced viability. The viability is increased if the Sxl^f1/+ progeny are also carrying msl-3¹, and is decreased if the da¹/+ mothers are also carrying msl-3¹. The frequency of sex transformation in Sxl^f1/+ female progeny derived from da¹/+ mothers is increased if either the mothers or the progeny are homozygous for msl-3¹. The frequency of morphological abnormalities seen in Sxl^f1/+ female progeny derived from da¹/+ mothers is greatly reduced if the progeny are also homozygous for msl-3¹.

(Uenoyama, 1984)

male-lethal

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

(Gladstein et al., 2010)

Enhancer of

Statement

Reference

msl-3[+]/msl-3¹ is an enhancer | maternal effect of partially lethal - majority die | dominant | female phenotype of sc^sisB-1, sisA[+]/sisA¹

(Gladstein et al., 2010)

Suppressor of

Statement

Reference

msl-3[+]/msl-3¹ is a suppressor | partially of increased cell number | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Other

Statement

Reference

Sxl[+]/Sxl^fP7B0, msl-3¹ has lethal | female | maternal effect phenotype

(Gladstein et al., 2010)

msl-3¹, sisA[+]/sisA¹ has lethal | female | maternal effect phenotype

(Gladstein et al., 2010)

Sxl[+]/Sxl^f9, msl-3¹ has lethal | female | maternal effect phenotype

(Gladstein et al., 2010)

msl-3¹, sc[+]/sc^sisB-1 has lethal | female | maternal effect | heat sensitive phenotype

(Gladstein et al., 2010)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

msl-3¹ is a non-enhancer of phenotype of msl-1^{C.hs.Tag:FLAG}

(Scott et al., 2000)

msl-3¹ is a non-enhancer of phenotype of msl-1^{Δ84.Tag:FLAG}

(Scott et al., 2000)

Suppressor of

Statement

Reference

msl-3[+]/msl-3¹ is a suppressor | partially of embryonic/larval hemocyte | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

NOT Suppressor of

Statement

Reference

msl-3¹ is a non-suppressor of phenotype of msl-1^{Δ84.Tag:FLAG}

(Scott et al., 2000)

msl-3¹ is a non-suppressor of phenotype of msl-1^{C.hs.Tag:FLAG}

(Scott et al., 2000)

msl-3¹ is a non-suppressor of germline cell phenotype of ovo^unspecified

(Oliver and Pauli, 1998)

Other

Statement

Reference

Sxl^f1, msl-3¹ has abdominal sternite 6 | female phenotype

(Uenoyama et al., 1982)

Sxl^f1, msl-3¹ has abdominal tergite 6 | female phenotype

(Uenoyama et al., 1982)

Sxl^f1, msl-3¹ has abdominal tergite 5 | female phenotype

(Uenoyama et al., 1982)

Sxl^f1, msl-3¹ has prothoracic metatarsus | female phenotype

(Uenoyama et al., 1982)

Sxl^f1, msl-3¹ has oviprovector phenotype

(Uenoyama et al., 1982)

Additional Comments

Genetic Interactions

Statement

Reference

Removing one copy of Rbp9^P2690 during oogenesis in combination with zygotic heterozygosity for Sxl^fP7B0 results in female lethality. No female lethality is seen in the reverse cross. This female lethality is suppressed when msl-3¹ is also zygotically removed.

(Zaharieva et al., 2015)

The viability of Sxl^fP7B0/+, sisA¹/+ or Sxl^f9/+ females is significantly reduced if they also carry one maternally-derived copy of msl-3¹.

The viability of sc^sisB-1/+ females raised at the non-permissive temperature of 29[o]C is significantly reduced if they also carry one maternally-derived copy of msl-3¹.

One maternally-derived copy of msl-3¹ significantly reduces the viability of sc^sisB-1 sisA¹/+ females so that they show almost complete lethality. One paternally-derived copy of msl-3¹ significantly reduces the viability of sc^sisB-1 sisA¹/+ females, although this zygotic effect is generally weaker than the maternal one.

(Gladstein et al., 2010)

The addition of msl-3¹ to flies containing a single copy of msl-1^{Δ84.T:Zzzz\FLAG} in weak heatshock conditions has no effect on the male viability phenotype seen in these flies. The addition of msl-3¹ to flies containing a single copy of msl-1^{C.hs.T:Zzzz\FLAG} in weak heatshock conditions has no effect on the male viability phenotype seen in these flies.

(Scott et al., 2000)

Does not suppress the germline death phenotype of ovo^unspecified females.

(Oliver and Pauli, 1998)

Sxl^f1/+ chromosomal females also homozygous or heterozygous msl-3¹ show variable transformation of sexually dimorphic structures to male characteristics. The frequency of transformation is not high, is higher in females homozygous (rather than heterozygous) for msl-3¹, and expression is incomplete. The frequency of transformation is higher in female flies with mothers homozygous msl-3¹. Sex combs are only seen in Sxl^f1/+ females homozygous for msl-3¹.

(Uenoyama et al., 1982)

Xenogenetic Interactions

Statement

Reference

One copy of msl-3¹ moderately suppresses the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cSa is expressed under the control of Scer\GAL4^Hml.Δ.

(Sinenko et al., 2010)

Complementation and Rescue Data

Rescued by

msl-3¹ is rescued by msl-3^WT.Tag:TAP

(Moore et al., 2010)

msl-3¹ is rescued by msl-3^EPAA.Tag:TAP

(Moore et al., 2010)

msl-3¹ is rescued by msl-3^+t.Tag:TAP

(Sural et al., 2008)

Partially rescued by

msl-3¹ is partially rescued by msl-3^Y31A.Tag:TAP

(Moore et al., 2010)

msl-3¹ is partially rescued by msl-3^ΔCD.Tag:TAP

(Sural et al., 2008)

msl-3¹ is partially rescued by msl-3^W59G.Tag:TAP

(Sural et al., 2008)

msl-3¹ is partially rescued by msl-3^{SYD62A.Tag:TAP}

(Sural et al., 2008)

Not rescued by

msl-3¹ is not rescued by msl-3^{LYT30A.Tag:TAP}

(Sural et al., 2008)

Comments

msl-3^{WT.T:Zzzz\TAP} rescues the male lethality caused by msl-3¹.

msl-3^{Y31A.T:Zzzz\TAP} partially rescues (31.9%) the male lethality caused by msl-3¹.

msl-3^{EPAA.T:Zzzz\TAP} rescues the male lethality caused by msl-3¹.

(Moore et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

5872

msl-3¹ red¹/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

Comments

H4Ac16, acetylated form of His4, is not localised to the X chromosome in male salivary gland nuclei.

(Bone et al., 1994)

A P-element maps near the 5' end of the adjacent gene, anon-65Ea but may not be responsible for the mutant phenotype: rescue constructs that do not include the site of insertion of the P-element can fully rescue the msl-3 mutant phenotype.

(Gorman et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

Msl3^p

(Fagegaltier et al., 2014)

mle(3)132

(Okuno et al., 1984, Uenoyama, 1984, Uenoyama et al., 1982)

mle3¹

(Scott et al., 2000)

msl-3¹

(Leatham-Jensen et al., 2019, Cuykendall et al., 2014, Gladstein et al., 2010, Sinenko et al., 2010, Doheny et al., 2008, Dahlsveen et al., 2006)

msl-3^P

(Lyman et al., 1997, Gorman et al., 1995, Bone et al., 1994)

msl-3^p

(Fagegaltier and Baker, 2004)

msl3¹

(McCarthy et al., 2022, Moore et al., 2010, Gorchakov et al., 2009, Sural et al., 2008, Larschan et al., 2007, Larschan et al., 2007, Bai et al., 2004)

msl3^P

(Kotlikova et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (30)

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