FlyBase Allele Report: Dmel\mrn[1]

General Information

Symbol

Dmel\mrn¹

Species

D. melanogaster

Name

FlyBase ID

FBal0012434

Feature type

allele

Associated gene

Dmel\mrn

Associated Insertion(s)

Carried in Construct

Genomic Maps

GBrowse JBrowse

Help me choose

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Matsuno et al., 2007)

Mutagen

ethyl methanesulfonate

(Fuller, 1986)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

point mutation

3L:15,579,434..15,579,434 [-]

Nucleotide change:

G15579434A

Amino acid change:

E340K | mrn-PA

Reported amino acid change:

E340K

Comment:

One of two missense mutations associated with this allele; site of nucleotide substitution in mutant inferred

by FlyBase based on reported amino acid change.

(Fregoso et al., 2007, Matsuno et al., 2007)

point mutation

3L:15,579,500..15,579,500 [-]

Nucleotide change:

G15579500C

Amino acid change:

A318P | mrn-PA

Reported amino acid change:

A318P

Comment:

One of two missense mutations associated with this allele; site of nucleotide substitution in mutant inferred

by FlyBase based on reported amino acid change.

(Matsuno et al., 2007)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Amino acid replacement: E340K.

(Fregoso et al., 2007, Matsuno et al., 2007)

Nucleotide substitution: G1018A.

(Fregoso et al., 2007)

Amino acid replacement: A318P.

(Matsuno et al., 2007)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle (with mrn^EP3605)

(Fregoso et al., 2007)

decreased body size | larval stage

(Fregoso et al., 2007)

decreased body size | pupal stage

(Fregoso et al., 2007)

decreased occurrence of cell division | larval stage

(Matsuno et al., 2007)

lethal

(Matsuno et al., 2007)

lethal (with mrn³)

(Fregoso et al., 2007)

lethal (with mrn⁵)

(Fregoso et al., 2007)

lethal (with mrn^EP572)

(Fregoso et al., 2007)

lethal | recessive

(Aguilar-Fuentes et al., 2008, Fregoso et al., 2007)

lethal - all die before end of larval stage

melanotic mass phenotype | larval stage

(Fregoso et al., 2007)

melanotic mass phenotype | larval stage (with mrn³)

(Fregoso et al., 2007)

partially lethal - majority die (with mrn^EP3605)

(Fregoso et al., 2007)

radiation sensitive | dominant

(Fregoso et al., 2007)

radiation sensitive | somatic clone

(Matsuno et al., 2007)

some die during larval stage

sterile (with mrn^EP3605)

(Fregoso et al., 2007)

Phenotype Manifest In

condensed chromosome (with mrn^EP3605)

(Fregoso et al., 2007)

egg chamber (with mrn^EP3605)

(Fregoso et al., 2007)

eye | somatic clone

(Matsuno et al., 2007)

eye disc | somatic clone

(Matsuno et al., 2007)

melanotic mass | larval stage

(Fregoso et al., 2007)

melanotic mass | larval stage (with mrn³)

(Fregoso et al., 2007)

nurse cell (with mrn^EP3605)

(Fregoso et al., 2007)

testis (with mrn^EP3605)

(Fregoso et al., 2007)

Detailed Description

Statement

Reference

Some homozygous larvae have melanotic tumours.

11.6% of mrn¹/mrn³ larvae have necrosis or melanotic tumours.

mrn^EP3605/mrn¹ larvae show general necrosis.

Heterozygous larvae are more sensitive to UVB irradiation compared to controls.

Homozygous larvae and pupae are smaller than controls.

Aberrant egg chambers are seen in mrn^EP3605/mrn¹ females and pycnotic nuclei are seen in nurse cells.

mrn^EP3605/mrn¹ males have defective testes, and spermatogenesis is either absent or not completed in most cases.

Mitotic spreads of mrn^EP3605/mrn¹ larval neuroblasts show a high rate of chromosome aberrations, including fragmentation of chromosomes and partial chromosome condensation.

(Fregoso et al., 2007)

Homozygous mutant clones induced in the eye using the eyFLP method are hardly detected in the adult eye. Homozygous mutant clones induced in the eye using the eyFLP method in a Minute/+ background are recovered in the eye disc, although these clones are much smaller than control clones.

Massive apoptosis is induced in homozygous clones in the eye disc 4 hours after UV irradiation of 500 J/m[2] (apoptosis is not induced in wild-type clones under these conditions).

BrdU incorporation is absent posterior to the morphogenetic furrow in homozygous clones in the eye disc.

(Matsuno et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mrn^EP3605/mrn¹ has sterile phenotype, suppressible | partially by Tfb5^Hsp83.PAF

(Aguilar-Fuentes et al., 2008)

mrn¹ has lethal | recessive phenotype, suppressible | partially by Hsap\GTF2H4^Hsp83.PF

(Fregoso et al., 2007)

NOT suppressed by

Statement

Reference

mrn¹ has lethal | recessive phenotype, non-suppressible by Tfb5^Hsp83.PAF

(Aguilar-Fuentes et al., 2008)

mrn^EP3605/mrn¹ has sterile phenotype, non-suppressible by Hsap\GTF2H4^Hsp83.PF

(Fregoso et al., 2007)

Phenotype Manifest In

Enhancer of

Statement

Reference

mrn¹ is an enhancer of phenotype of βTub85D^unspecified

(Fuller, 1986)

Additional Comments

Genetic Interactions

Statement

Reference

Fails to complement class I alleles at the βTub85D locus.

(Fuller, 1986)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

mrn^EP3605

(Fregoso et al., 2007)

Fails to complement

mrn^EP572

(Fregoso et al., 2007)

mrn³

(Fregoso et al., 2007)

mrn⁵

(Fregoso et al., 2007)

Rescued by

mrn^EP3605/mrn¹ is rescued by mrn^Hsp83.PF

(Fregoso et al., 2007)

Partially rescued by

mrn¹ is partially rescued by mrn^Hsp83.PF

(Fregoso et al., 2007)

mrn¹ is partially rescued by mrn^Hsp83.PF

(Fregoso et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a mutation that fails to complement class I alleles at the βTub85D locus (either βTub85D³ or βTub85D^Drv1) but does not map to the βTub85D locus.

(Fuller, 1986)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

mrn¹

(Aguilar-Fuentes et al., 2008, Fregoso et al., 2007, Matsuno et al., 2007)

mrn^nc16

nc16

(Fuller, 1986)

nc¹⁶

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

Open Close