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General Information
Symbol
Dmel\ms(3)K811
Species
D. melanogaster
Name
FlyBase ID
FBal0012544
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
K811
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
insertion
Comment:

A mutation caused by an imprecise excision event; 38 bases of P element remain as well as the 8bp target site duplication. The insertion results in a premature stop codon after the first 15 residues of the predicted protein.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

38bp insertion near the 5' end of ms(3)K81. The insertion consists of a 30bp match to the inverted terminal repeats of the P-element and an 8bp duplication of the insertion site. The insertion results in a premature stop codon after the first 15 residues of the predicted protein.

Insertion components
P{?'}ms(3)K811
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Paternal chromosomes are unable to separate sister chromatids during the first zygotic division of embryos derived from wild-type females crossed to homozygous males and they form a chromatin bridge that stretches between the spindle poles. The maternally derived chromatids separate normally during the first zygotic division. In metaphase of the second zygotic division, paternal chromatin typically bridges between the two haploid nuclei that contain maternally derived chromosomes.

When wild type females are mated to homozygous ms(3)K811 males the progeny fall into two groups: most arrest in early nuclear cycles and the rest develop as haploids and die before hatching. The events of sperm decondensation, pronuclear migration, and alignment leading to the first mitotic division are not different from wild type, but anaphase is abnormal. Chromosome bridges are stretched between the poles. Anaphase bridges are also observed in subsequent mitoses.

Homozygous males produce motile sperm capable of fertilizing eggs, but defective in pronuclear function. Development of fertilized eggs initiated; arrested after several nuclear divisions in three-fourths of the embryos, the remainder developing beyond blastoderm and proving to be haploid upon cytological examination. Nuclear division cycle protracted compared to wild type and the syncytial blastoderm nuclei undergo an extra division to produce twice the normal density prior to cellularization (Edgar, Kiehle and Schuberger, 1986). A small fraction of the eggs diploidize and yield viable daughters, mostly by fusion of products of the first meiotic division (85%); fusion of products of second meiotic division rare or nonexistent. Incidence of gynogenetic diploids becomes appreciable in crosses to a stock designated G9 by Fuyama (1986). male-sterile

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (9)