Open Close
General Information
Symbol
Dmel\mus304D1
Species
D. melanogaster
Name
FlyBase ID
FBal0012647
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
mus3041
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C18111608T

Amino acid change:

Q539term | mus304-PA

Reported amino acid change:

Q539term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Q539term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

mus304D1/Df(3L)W4 eye discs show severe defects in hydroxyurea-induced G2 arrest.

mus304D1/mus304D3 mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 32oC or 38oC.

In the absence of irradiation, mus304D1 eye discs have normal morphology and a wild-type number and distribution of mitotic cells. The number of mitotic cells is unchanged following irradiation, in contrast to wild-type discs where very few cells are in M phase after irradiation. The presence of mitotic cells in these irradiated discs is not a result of either a delayed onset of or a rapid recovery from cell cycle arrest. The defect in the DNA damage checkpoint is also seen in wing discs. mus304D1/Df(3L)W4 and mus304D1/Df(3L)x37 animals have macrochaetae defects (probably due genomic instability resulting in the loss of genetic material at haploinsufficient Minute loci). mus304D1/mus304D2 flies have wild-type eyes. The frequency of chromosome aberrations following X-ray irradiation is threefold greater in mus304D1/Df(3L)W4 cells than in wild type. The spontaneous frequency of chromosomal aberrations in mus304D1/Df(3L)W4 animals is more than 10-fold greater than in wild type. The majority of aberrations are chromatid or isochromatid breaks. Loss of heterozygosity is elevated compared to wild-type in homozygous, mus304D1/Df(3L)W4 and mus304D1/Df(3L)x37 animals. mus304D1/Df(3L)W4 females show a meiotic chromosome segregation defect, which is manifested as a moderate increase in female exceptional progeny (derived from diplo-X ova) and a pronounced increase in male exceptional progeny (derived from nullo-X ova).

Flies are not sensitive to X rays.

Hypersensitive to methyl methanesulfonate. Sensitive to 0.008% HN2.

female-fertile

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Other
Phenotype Manifest In
Enhancer of
Statement
Reference

mus304D1/mus304D1 is an enhancer of chromosome & neuroblast | third instar larval stage 2 phenotype of mre11unspecified

mus304D3/mus304D1 is an enhancer of chromosome & neuroblast | third instar larval stage 2 phenotype of tefuunspecified

mus304D1/mus304D1 is an enhancer of chromosome & neuroblast | third instar larval stage 2 phenotype of tefuunspecified

NOT Suppressor of
Statement
Reference

mus304D1 is a non-suppressor of egg chorion phenotype of okrAA/okrRU

mus304D1 is a non-suppressor of dorsal appendage phenotype of okrAA/okrRU

mus304D1 is a non-suppressor of egg chorion phenotype of spn-BBU

mus304D1 is a non-suppressor of dorsal appendage phenotype of spn-BBU

mus304D1/mus3046 is a non-suppressor of egg chorion phenotype of spn-BBU

mus304D1/mus3046 is a non-suppressor of dorsal appendage phenotype of spn-BBU

Other
Additional Comments
Genetic Interactions
Statement
Reference

The chromosome fusion phenotype seen in third instar larval neuroblasts of tefuunspecified mutants is enhanced by mus304D1/mus304D3 and strongly enhanced by mus304D1/mus304D1.

If mus304D1/mus304D2 flies are also heterozygous for E(mus304)1 they have rough eyes and abnormal or missing bristles.

mus304D1/mus304D3 has no effect on the frequency of X-Y chromosome nondisjunction seen in Df(1)X-1-53B males, but shows a significant enhancement of meiotic drive in these flies compared to controls.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Boyd.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (14)