Allele Dmel\mus304^D2

General Information
Symbol	Dmel\mus304^D2	Species	D. melanogaster
Name		FlyBase ID	FBal0012648
Feature type	allele	Associated gene	Dmel\mus304
Also Known As	mus304²

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Boyd et al., 1981)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3L:18,105,442..18,105,442 na_change=G18105442A comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified) evidence=experimental pr_change=W759@\|mus304-PA reported_pr_change=W759@ (Brodsky et al., 2000)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: W759@. (Brodsky et al., 2000)
Cytology
Phenotypic Data
Phenotypic Class
	chemical sensitive \| recessive (Boyd et al., 1981) visible \| recessive (Brodsky et al., 2000)
Phenotype Manifest In
	macrochaeta (Brodsky et al., 2000)
Detailed Description
	Statement Reference mus304[D2] mutant cells exhibit DNA breaks and telomere fusions in metaphase and anaphase at almost the same level as wild-type cells. (Oikemus et al., 2006) mus304^D2 animals show a strong defect in a DNA damage checkpoint assay in the eye disc. mus304^D2/Df(3L)W4 animals have macrochaetae defects (probably due genomic instability resulting in the loss of genetic material at haploinsufficient Minute loci). mus304^D1/mus304^D2 flies have wild-type eyes. Loss of heterozygosity is elevated compared to wild-type in mus304^D2/Df(3L)W4 animals. (Brodsky et al., 2000) Hypersensitive to methyl methanesulfonate. Sensitive to 0.008% HN2. (Boyd et al., 1981) female-sterile
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Df(3L)W4/mus304^D2 has visible phenotype, enhanceable by E(mus304)[+]/DNApol-α180^Emus304 (Brodsky et al., 2000) mus304^D2 has mitotic cell cycle defective phenotype, enhanceable by nbs¹ (Oikemus et al., 2006) mus304^D2 has mitotic cell cycle defective phenotype, enhanceable by tefu¹ (Oikemus et al., 2006)
Enhancer of
Statement Reference mus304^D2 is an enhancer of mitotic cell cycle defective phenotype of nbs¹ (Oikemus et al., 2006) mus304^D2 is an enhancer of mitotic cell cycle defective phenotype of tefu¹ (Oikemus et al., 2006)
Other
Statement Reference DNApol-α180^Emus304, mus304^D1/mus304^D2 has visible phenotype (Brodsky et al., 2000) E(mus304)[+]/DNApol-α180^Emus304, mus304^D1/mus304^D2 has visible phenotype (Brodsky et al., 2000)
Phenotype Manifest In
Enhanced by
Statement Reference Df(3L)W4/mus304^D2 has macrochaeta phenotype, enhanceable by E(mus304)[+]/DNApol-α180^Emus304 (Brodsky et al., 2000)
Other
Statement Reference DNApol-α180^Emus304, mus304^D1/mus304^D2 has eye phenotype (Brodsky et al., 2000) DNApol-α180^Emus304, mus304^D1/mus304^D2 has macrochaeta phenotype (Brodsky et al., 2000) DNApol-α180^Emus304, mus304^D1/mus304^D2 has ommatidium phenotype (Brodsky et al., 2000) E(mus304)[+]/DNApol-α180^Emus304, mus304^D1/mus304^D2 has eye phenotype (Brodsky et al., 2000) E(mus304)[+]/DNApol-α180^Emus304, mus304^D1/mus304^D2 has macrochaeta phenotype (Brodsky et al., 2000) E(mus304)[+]/DNApol-α180^Emus304, mus304^D1/mus304^D2 has ommatidium phenotype (Brodsky et al., 2000)
Additional Comments
Genetic Interactions
Statement Reference nbs[1] mus304[D2] double mutant cells exhibit more DNA breaks and telomere fusions in metaphase and anaphase than in wild-type cells. tefu[1] mus304[D2] double mutant cells exhibit more DNA breaks and telomere fusions in metaphase and anaphase than in wild-type cells. (Oikemus et al., 2006) The frequency of macrochaetae defects in mus304^D2/Df(3L)W4 animals is increased by one copy of E(mus304)¹. If mus304^D1/mus304^D2 flies are also heterozygous for E(mus304)¹ they have rough eyes and abnormal or missing bristles. (Brodsky et al., 2000)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer	Boyd.
	Separable from: E(mus304)¹. (Brodsky et al., 2000)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	mus304² (Abdu et al., 2002, Brodsky et al., 2000) mus304^D2 (Oikemus et al., 2006)
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Oikemus et al., 2006, PLoS Genet. 2(5): e71 Epigenetic telomere protection by Drosophila DNA damage response pathways. [FBrf0194327] Abdu et al., 2002, Curr. Biol. 12(19): 1645--1651 Activation of a meiotic checkpoint during Drosophila oogenesis regulates the translation of Gurken through Chk2/Mnk. [FBrf0151847] Brodsky et al., 2000, Genes Dev. 14(6): 666--678 mus304 encodes a novel DNA damage checkpoint protein required during Drosophila development. [FBrf0127025] Boyd et al., 1981, Genetics 97(34): 607--623 Third-chromosome mutagen-sensitive mutants of Drosophila melanogaster. [FBrf0036532]

Allele Dmel\mus304D2

Allele Dmel\mus304^D2