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General Information
Symbol
Dmel\PolQD5
Species
D. melanogaster
Name
FlyBase ID
FBal0012659
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C12709745T

Amino acid change:

P781L | DNApol-theta-PA

Reported amino acid change:

P781L

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: P781L.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

mus308D5/Df(3R)Exel6166 larvae are unable to survive exposure to 0.005% mechlorethamine (nitrogen mustard).

mus308D5/Df(3R)Exel6166 animals are able to survive exposures of as high as 4000rads of ionising radiation.

Studies of excision and repair events of P{hswa} in mus308D5/mus308Sz29 males indicate that DNA repair synthesis during synthesis-dependent strand annealing (SDSA) is unaffected in the mutants but that the mutants are defective in end-joining repair of DNA double-strand breaks. There is a substantial increase in the percentage of deletion-associated repair events isolated from mus308D5 mutant males relative to those isolated from wild-type males.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference

PolQD5 is an enhancer of radiation sensitive phenotype of spn-A3

Other
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

spn-A3 mus308D5 double mutant animals are extremely sensitive to ionising radiation, with almost no survival of the mutants after exposure to 125rads ionising radiation.

There is a decrease in the percentage of end-joining repair products in studies of excision and repair events of P{hswa} in spn-A093A mus308D5/spn-A3 mus308ZIII-2003 double mutant males compared to the percentage seen in spn-A single mutants. The double mutants show a substantial increase in the percentage of deletion-associated repair events isolated compared to controls, as occurs in mus308 single mutants. These males show increased sterility.

There is a decrease in the percentage of end-joining repair products in studies of excision and repair events of P{hswa} in spn-A093A mus308D5/spn-A093A mus308ZIII-3294 double mutant males compared to the percentage seen in spn-A single mutants. The double mutants show an increase in the percentage of deletion-associated repair events isolated compared to controls, as occurs in mus308 single mutants. These males show increased sterility.

mus308D1/mus308D5 has no effect on the frequency of X-Y chromosome nondisjunction seen in Df(1)X-1-53B males, but shows a significant enhancement of meiotic drive in these flies compared to controls.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Boyd.

Separable from: a second unidentified mutagen-sensitive mutant allele.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
DNApol-θD5
PolQD5
mus308D5
Name Synonyms
Secondary FlyBase IDs
    References (4)