Allele Dmel\mys¹

General Information
Symbol	Dmel\mys¹	Species	D. melanogaster
Name		FlyBase ID	FBal0012678
Feature type	allele	Associated gene	Dmel\mys

Allele class	amorphic allele - genetic evidence, loss of function allele
Mutagen	32P
Recent Updates
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FB2012_01	Controlled Vocabulary Terms embryonic/larval somatic muscle embryonic stage 16 References Nguyen et al., 2007, J. Cell Biol. 179(2): 219--227 Unknown 32p radiolabel
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	amorphic allele - genetic evidence (Bunch et al., 1992) loss of function allele (Buttgereit, 1996)
Mutagen	32P (Digan et al., 1986)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Deletion of at least 1kb beginning in central portion of coding region and extending towards 3' of gene, probably beyond the 3' end of the gene. Faint residual band on immunoblots (as for Df(1)C128) where product is thought to be a remnant from the maternal supply. (Bunch et al., 1992)
Cytology	Polytene chromosomes normal.
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage \| recessive (Bunch et al., 1992) neuroanatomy defective (Stevens and Jacobs, 2002) neuroanatomy defective \| recessive \| somatic clone (Billuart et al., 2001)
Phenotype Manifest In
	dorsal closure embryo (Reed et al., 2004) embryonic/larval somatic muscle \| embryonic stage 16 (Nguyen et al., 2007) extended germ band embryo (Reed et al., 2004) fascicle (Stevens and Jacobs, 2002) mushroom body vertical lobe \| somatic clone (Billuart et al., 2001)
Detailed Description
	Statement Reference The somatic muscles are detached and shrunken in stage 16 homozygous embryos. These detached muscles do not show any significant apoptotic signals. (Nguyen et al., 2007) Embryos show weak germ band retraction and dorsal closure defects - embryos have a small dorsal hole. Those embryos that fail germ band retraction exhibit apparently normal phase I interaction, however phase II membrane apposition fails completely. Most striking is the failure of the dorsal anterior region of the amnioserosa to initiate contact with the yolk sac membrane, with subsequent high penetrance failure of dorsal closure. (Reed et al., 2004) The three longitudinal fascicles, as visible with Fas2, are intact in mys¹ mutants. Midline fusions of the most medial tract are seen in some segments. (Stevens and Jacobs, 2002) 12/52 neuroblast clones in the mushroom body show obvious overextension of dorsal lobe axons. Both overextension of thin axon bundles near the tip of the dorsal lobe and overextension of a large portion of the dorsal axons is seen. (Billuart et al., 2001) No anti-β_PS staining at muscle attachment sites. (Bunch et al., 1992)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference mys¹ has neuroanatomy defective phenotype, enhanceable by sli² (Stevens and Jacobs, 2002)
Enhancer of
Statement Reference mys¹/mys[+] is an enhancer of visible \| dominant phenotype of sog^EP7 (Araujo et al., 2003) mys¹/mys[+] is an enhancer of visible \| dominant phenotype of sog^EP11 (Araujo et al., 2003)
NOT Enhancer of
Statement Reference mys¹/mys[+] is a non-enhancer of cell polarity defective phenotype of Scer\GAL4^hs.2sev, shg^{dCR3h.Scer\UAS.T:Avic\GFP-rs} (Mirkovic and Mlodzik, 2006) mys¹/mys[+] is a non-enhancer of planar polarity defective phenotype of Scer\GAL4^hs.2sev, nmo^Scer\UAS.cUa (Mirkovic et al., 2011) mys¹ is a non-enhancer of cell polarity defective phenotype of S⁰⁵⁶⁷¹ (Brown and Freeman, 2003) mys¹ is a non-enhancer of visible phenotype of Ppt1^Scer\UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Buff et al., 2007)
Suppressor of
Statement Reference mys¹/mys[+] is a suppressor \| partially of neuroanatomy defective phenotype of Scer\GAL4^elav.PLu, p130CAS^{Scer\UAS.T:Hsap\MYC} (Huang et al., 2007) mys¹ is a suppressor of neuroanatomy defective phenotype of RhoGAPp190^{dsRNA.GAP.Scer\UAS}, Scer\GAL4^ey-OK107 (Billuart et al., 2001)
NOT Suppressor of
Statement Reference mys¹/mys[+] is a non-suppressor of cell polarity defective phenotype of Scer\GAL4^hs.2sev, shg^{dCR3h.Scer\UAS.T:Avic\GFP-rs} (Mirkovic and Mlodzik, 2006) mys¹/mys[+] is a non-suppressor of planar polarity defective phenotype of Scer\GAL4^hs.2sev, nmo^Scer\UAS.cUa (Mirkovic et al., 2011) mys¹ is a non-suppressor of cell polarity defective phenotype of S⁰⁵⁶⁷¹ (Brown and Freeman, 2003) mys¹ is a non-suppressor of visible phenotype of Ppt1^Scer\UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Buff et al., 2007)
Other
Statement Reference Df(3L)Exel6083/+, mys¹ has neuroanatomy defective \| dominant phenotype (Huang et al., 2007) mys¹/mys[+], p130CAS¹ has semi-lethal \| embryonic stage phenotype (Tikhmyanova et al., 2010)
Phenotype Manifest In
Enhanced by
Statement Reference mys¹ has dorsal closure embryo phenotype, enhanceable by Bsg^NP6293/Bsg[+] (Reed et al., 2004) mys¹ has embryonic/larval optic stalk phenotype, enhanceable by Fak56D^CG1/Fak56D[+] (Murakami et al., 2007) mys¹ has fascicle phenotype, enhanceable by sli² (Stevens and Jacobs, 2002)
Enhancer of
Statement Reference mys¹/mys[+] is an enhancer of embryonic/larval optic stalk phenotype of Fak56D^CG1 (Murakami et al., 2007) mys¹/mys[+] is an enhancer of extended germ band embryo phenotype of EcR^{hs.T:Scer\GAL4} (Kozlova and Thummel, 2003) mys¹/mys[+] is an enhancer of heart primordium phenotype of sli² (MacMullin and Jacobs, 2006) mys¹/mys[+] is an enhancer of oocyte phenotype of Lar^5.5/Lar^13.2 (Bateman et al., 2001) mys¹/mys[+] is an enhancer of wing vein phenotype of sog^EP7 (Araujo et al., 2003) mys¹/mys[+] is an enhancer of wing vein phenotype of sog^EP11 (Araujo et al., 2003)
NOT Enhancer of
Statement Reference mys¹/mys[+] is a non-enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^Scer\UAS.cUa (Mirkovic et al., 2011) mys¹/mys[+] is a non-enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, shg^{dCR3h.Scer\UAS.T:Avic\GFP-rs} (Mirkovic and Mlodzik, 2006) mys¹ is a non-enhancer of eye phenotype of Ppt1^Scer\UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Buff et al., 2007) mys¹ is a non-enhancer of ommatidium phenotype of S⁰⁵⁶⁷¹ (Brown and Freeman, 2003)
Suppressor of
Statement Reference mys¹/mys[+] is a suppressor \| partially of intersegmental nerve phenotype of Scer\GAL4^elav.PLu, p130CAS^{Scer\UAS.T:Hsap\MYC} (Huang et al., 2007) mys¹/mys[+] is a suppressor \| partially of presumptive embryonic/larval central nervous system phenotype of Scer\GAL4^elav.PLu, p130CAS^{Scer\UAS.T:Hsap\MYC} (Huang et al., 2007) mys¹/mys[+] is a suppressor \| partially of segmental nerve phenotype of Scer\GAL4^elav.PLu, p130CAS^{Scer\UAS.T:Hsap\MYC} (Huang et al., 2007) mys¹ is a suppressor of mushroom body vertical lobe phenotype of RhoGAPp190^{dsRNA.GAP.Scer\UAS}, Scer\GAL4^ey-OK107 (Billuart et al., 2001)
NOT Suppressor of
Statement Reference mys¹/mys[+] is a non-suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^Scer\UAS.cUa (Mirkovic et al., 2011) mys¹/mys[+] is a non-suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, shg^{dCR3h.Scer\UAS.T:Avic\GFP-rs} (Mirkovic and Mlodzik, 2006) mys¹ is a non-suppressor of eye phenotype of Ppt1^Scer\UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Buff et al., 2007) mys¹ is a non-suppressor of ommatidium phenotype of S⁰⁵⁶⁷¹ (Brown and Freeman, 2003)
Other
Statement Reference Df(3L)Exel6083/+, mys¹ has intersegmental nerve phenotype (Huang et al., 2007) Df(3L)Exel6083/+, mys¹ has presumptive embryonic/larval central nervous system phenotype (Huang et al., 2007) Df(3L)Exel6083/+, mys¹ has segmental nerve phenotype (Huang et al., 2007)
Additional Comments
Genetic Interactions
Statement Reference Approximately 24% of mys[1]/+; p130CAS[1]/p130CAS[1] double mutants emerge as adults. (Tikhmyanova et al., 2010) mys[1]/+ ; Df(3L)Exel6083/+ double heterozygotes show axon guidance defects characteristic of increased fasciculation in the ISNb pathway (75.4% of hemisegments), in the SNa pathway (41.8% of hemisegments) and in the central nervous system (40.0% of hemisegments). These defects are not seen in either single heterozygote. The axon guidance defects seen in the ISNb pathway, SNa pathway and central nervous system of embryos expressing p130CAS[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[elav.PLu] are significantly rescued by mys[1]/+. (Huang et al., 2007) Fak56D^CG1 mys¹ double heterozygotes show more severe defects in optic stalk morphology than either single heterozygote, which each show only slight defects in optic stalk formation. (Murakami et al., 2007) mys¹/+; sli²/+ embryos show delayed migration of cardial cells, and clumping of heart cells. (MacMullin and Jacobs, 2006) A mys¹/+ background does not affect the Scer\GAL4^hs.2sev>shg^{dCR3h.Scer\UAS.T:Avic\GFP-rs} ommatidial phenotype. (Mirkovic and Mlodzik, 2006) Homozygous mys¹ embryos also heterozygous for Bsg^NP6293 show an enhancement of the dorsal hole phenotype seen in mys¹ embryos. (Reed et al., 2004) Heterozygous mys¹ has no effect on the S⁰⁵⁶⁷¹/+ ommatidial polarity phenotype. (Brown and Freeman, 2003) S^48-5/mys¹ mutants show 7.64%+-3.82 misrotated ommatidia compared to 9.55%+-1.43 seen in S^48-5 mutants alone. (Gaengel and Mlodzik, 2003) The penetrance and expressivity of the germ band retraction defect seen in embryos in which EcR^{hs.T:Scer\GAL4} is expressed using heat shock for 3 to 5 hours after egg laying is enhanced by mys¹/+. (Kozlova and Thummel, 2003) In mys¹/+ sli²/+ double heterozygous mutants the frequency of midline guidance errors is increased over the level observed in mys¹ homozygous mutants. Apart from midline axon crossings in one-third of the segments, the longitudinal tracts (as visible with Fas2) appear normal. (Stevens and Jacobs, 2002) The addition of mys¹/+ to Lar^5.5/Lar^13.2 increases the penetrance of the oocyte elongation phenotype from 14.1% to about 40%. (Bateman et al., 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	mys^ts1 (Bunch et al., 1992) mys^ts3 (Bunch et al., 1992)
Comments
Stocks ( 3 )
Bloomington	59 mys¹/FM4 23862 mys¹ P{neoFRT}19A/FM7c
Kyoto	105704 mys¹/FM4
Notes on Origin
Discoverer	Poulson Oct. 1948.

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	l(1)48j l(1)mys (Digan et al., 1986) mys¹ (MacMullin and Jacobs, 2006, Mirkovic and Mlodzik, 2006, Nguyen et al., 2007, Murakami et al., 2007, Mirkovic et al., 2011, Buff et al., 2007, Huang et al., 2007, Popodi et al., 2010-, Tikhmyanova et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 27 )

Generate a list of	All references relating to this allele ( 27 )

List References by type	Research paper ( 24 ) Supplementary material ( 1 ) Personal communication to FlyBase ( 1 ) Abstract ( 1 )
Recent research papers ( 1 )
	Tikhmyanova et al., 2010, PLoS ONE 5(8): e12369 Dcas supports cell polarization and cell-cell adhesion complexes in development. [FBrf0211678] Show all research papers ...

Allele Dmel\mys1

Allele Dmel\mys¹