A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Allele Dmel\mys8

General Information
SymbolDmel\mys8SpeciesD. melanogaster
NameFlyBase IDFBal0012685
Feature typealleleAssociated geneDmel\mys
Also Known Asmysnj42, mysnj, nj42
Allele classheat sensitive loss of function allele
Mutagenethyl methanesulfonate
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Description
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FB2013_03
FB2013_02
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hide Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
Progenitor genotype
Nature of the lesion
Statement
Reference
May contain regulatory mutation as lesion not found within exons or splice sites of mys.
Indistinguishable from wild type on Southern blot. Immunoblots indicate βPS protein levels are comparable to wild type (within a factor of two).
Cytology
hide Phenotypic Data
hide Phenotypic Class
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
10% of mutant males show wing blisters.
Hub cells fail to localise to the apical tip of the testis in homozygous males.
Avoidance of benzaldehyde is blunted in mutant animals compared to wild type. mys[ts1]/mys[8] and mys[ts2]/mys[8] flies show normal odour avoidance behaviour.
0.3% of hemizygous mutant wings show a wing blister phenotype.
mys8 larvae show a reduced olfactory response to isoamyl acetate and n-butanol, but are unaffected in their response to ethyl acetate and benzaldehyde. mys8/mysolfC-A459.1F1 flies survive and show no defect in jumping ability. They show a reduced olfactory response to isoamyl acetate but respond normally to ethyl acetate.
Some venation abnormalities are seen in homozygous mutant adults. mys8/Y flies have a low frequency of wing blisters (=<10%).
2% of hemizygous males have wing blisters.
Wing blisters are seen in approximately 5-10% of hemizygotes, and approximately 30-50% of hemizygotes have held-out wings.
Retinal development begins to break down at 55% of pupal life: one half of the R8's cell bodies have fallen though the retinal floor coming to lie underneath the cone cell plate, interrupting cone cell plate contacts. By 67% of pupal life the pigment cell feet have pulled away from the grommet extracellular membrane, opening holes around the cone cell plate. By the adult stage the retinal floor is completely disrupted. Rhabdomeres buckle. Temperature shift experiments reveal that the mys function is required during cone cell plate formation.
Wings are held out at an angle of 90o to the body, when flies are reared at 29oC.
mysxR04/mys8 flies die at 25oC but survive at 18oC, and have inflated or blistered wings and often show a gap between the pigment cell feet constituting the fenestrated membrane and the layer of pigmented subretinal cells found below the basement membrane. The viable mys8 adults have held-out wings, a phenotype which is enhanced at 29oC. mys8 males have blistered wings.
Homozygotes can survive at 18oC, but their number decreases gradually as the rearing temperature is raised to 28oC where none survive.
Homozygotes show no jumping response and severe muscular defects.
Homozygous wing is wild type in appearance but sometimes held abnormally away from the body due to severe wasting of the mesothoracic and metathoracic tergotrochanteral muscles. This phenotype is enhanced in heterozygotes. In combination with Df(1)C128 wings display morphogenetic defects, wing blisters, the formation of extra crossveins between L2 and L3 and abnormal 'delta' thickenings at vein intersections. Heterozygotes with mysxR04 are lethal. Double hemizygous and homozygous mutants with mysxR04 are lethal.
adults survive as nonjumpers
 
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hide Phenotypic Class
hideEnhanced by
Statement
Reference
mys8/mysG has visible phenotype, enhanceable by bs[+]/bsunspecified
mys8 has visible | recessive phenotype, enhanceable by 2R-F[+]/2R-F2R-22
mys8 has visible | recessive phenotype, enhanceable by 2R-L[+]/2R-L84-2
mys8 has visible | recessive phenotype, enhanceable by auk2R-4/auk[+]
mys8 has visible | recessive phenotype, enhanceable by aukunspecified/auk[+]
mys8 has visible | recessive phenotype, enhanceable by blo[+]/blounspecified
mys8 has visible | recessive phenotype, enhanceable by blo2R-17/blo[+]
mys8 has visible | recessive phenotype, enhanceable by bs[+]/bs2R-19
mys8 has visible | recessive phenotype, enhanceable by bs[+]/bsunspecified
mys8 has visible | recessive phenotype, enhanceable by bsV100/bs[+]
mys8 has visible | recessive phenotype, enhanceable by bub2L-2/bub[+]
mys8 has visible | recessive phenotype, enhanceable by kiwi2R-3/kiwi[+]
mys8 has visible | recessive phenotype, enhanceable by kiwiunspecified/kiwi[+]
mys8 has visible | recessive phenotype, enhanceable by mamunspecified/mam[+]
mys8 has visible | recessive phenotype, enhanceable by moa[+]/moaV50
mys8 has visible | recessive phenotype, enhanceable by pio[+]/pio2R-16
mys8 has visible | recessive phenotype, enhanceable by pio[+]/piounspecified
mys8 has visible | recessive phenotype, enhanceable by puri[+]/puriV4
mys8 has visible | recessive phenotype, enhanceable by rhea[+]/rhea1
mys8 has visible | recessive phenotype, enhanceable by rhea[+]/rhea2
mys8 has visible | recessive phenotype, enhanceable by shot65-2/shot[+]
mys8 has visible | recessive phenotype, enhanceable by shotunspecified/shot[+]
mys8 has visible | recessive phenotype, enhanceable by stck[+]/stck3R-17
mys8 has visible | recessive phenotype, enhanceable by stck[+]/stckunspecified
hideEnhancer of
Statement
Reference
mys8/mys[+] is an enhancer of visible | dominant phenotype of sogEP7
mys8/mys[+] is an enhancer of visible | dominant phenotype of sogEP11
hideSuppressor of
Statement
Reference
hideOther
Statement
Reference
hide Phenotype Manifest In
hideEnhanced by
Statement
Reference
mys8/mysG has wing phenotype, enhanceable by bs[+]/bsunspecified
mys8 has hub cell phenotype, enhanceable by Lasp[+]/Lasp1
mys8 has phenotype, enhanceable by Dlunspecified
mys8 has wing phenotype, enhanceable by 2R-F[+]/2R-F2R-22
mys8 has wing phenotype, enhanceable by 2R-L[+]/2R-L84-2
mys8 has wing phenotype, enhanceable by auk2R-4/auk[+]
mys8 has wing phenotype, enhanceable by aukunspecified/auk[+]
mys8 has wing phenotype, enhanceable by blo[+]/blounspecified
mys8 has wing phenotype, enhanceable by blo2R-17/blo[+]
mys8 has wing phenotype, enhanceable by bs[+]/bs2R-19
mys8 has wing phenotype, enhanceable by bs[+]/bsunspecified
mys8 has wing phenotype, enhanceable by bsV100/bs[+]
mys8 has wing phenotype, enhanceable by bub2L-2/bub[+]
mys8 has wing phenotype, enhanceable by crol3ex5
mys8 has wing phenotype, enhanceable by crolk08217
mys8 has wing phenotype, enhanceable by EcRC300Y
mys8 has wing phenotype, enhanceable by EcRM554fs
mys8 has wing phenotype, enhanceable by if3
mys8 has wing phenotype, enhanceable by kiwi2R-3/kiwi[+]
mys8 has wing phenotype, enhanceable by kiwiunspecified/kiwi[+]
mys8 has wing phenotype, enhanceable by mamunspecified/mam[+]
mys8 has wing phenotype, enhanceable by moa[+]/moaV50
mys8 has wing phenotype, enhanceable by pio[+]/pio2R-16
mys8 has wing phenotype, enhanceable by pio[+]/piounspecified
mys8 has wing phenotype, enhanceable by puri[+]/puriV4
mys8 has wing phenotype, enhanceable by rhea[+]/rhea1
mys8 has wing phenotype, enhanceable by rhea[+]/rhea2
mys8 has wing phenotype, enhanceable by shot65-2/shot[+]
mys8 has wing phenotype, enhanceable by shotunspecified/shot[+]
mys8 has wing phenotype, enhanceable by stck[+]/stck3R-17
mys8 has wing phenotype, enhanceable by stck[+]/stckunspecified
mys8 has wing phenotype, enhanceable by wb[+]/wb4Y18
hideNOT Enhanced by
Statement
Reference
mys8 has wing phenotype, non-enhanceable by auk[+]/aukV162
mys8 has wing phenotype, non-enhanceable by aukV106/auk[+]
mys8 has wing phenotype, non-enhanceable by blo[+]/bloV134
mys8 has wing phenotype, non-enhanceable by bloV99/blo[+]
mys8 has wing phenotype, non-enhanceable by bs[+]/bsV121
mys8 has wing phenotype, non-enhanceable by cassE44/cass[+]
mys8 has wing phenotype, non-enhanceable by crol04418
mys8 has wing phenotype, non-enhanceable by crol06470
mys8 has wing phenotype, non-enhanceable by how[+]/howT1
mys8 has wing phenotype, non-enhanceable by how[+]/howT90
mys8 has wing phenotype, non-enhanceable by LanA9-32/LanA[+]
mys8 has wing phenotype, non-enhanceable by moaV88/moa[+]
mys8 has wing phenotype, non-enhanceable by puri[+]/puriV15
mys8 has wing phenotype, non-enhanceable by pyg[+]/pygE16
mys8 has wing phenotype, non-enhanceable by pygE50/pyg[+]
mys8 has wing phenotype, non-enhanceable by shot[+]/shotV167
mys8 has wing phenotype, non-enhanceable by shotV104/shot[+]
mys8 has wing phenotype, non-enhanceable by tak[+]/takV27
mys8 has wing phenotype, non-enhanceable by takV42/tak[+]
mys11/mys8 has wing phenotype, non-enhanceable by pot[+]/potP3
hideEnhancer of
Statement
Reference
mys8/mys[+] is an enhancer of wing vein phenotype of sogEP7
mys8/mys[+] is an enhancer of wing vein phenotype of sogEP11
mys8 is an enhancer of phenotype of bsunspecified
mys8 is an enhancer of wing phenotype of if3
hideOther
Statement
Reference
cassE48/cass[+], mys8 has wing phenotype
hide Additional Comments
hide Genetic Interactions
Statement
Reference
The frequency of wing blisters seen in mys[8]/Y flies is increased to 54% if they are also expressing tnc[dsRNA.Scer\UAS.cFa] under the control of Scer\GAL4[Act5C.PI], and the overall frequency of wing defects is increased to 68%.
mys[8] ; Lasp[1]/+ males show a stronger hub cell mislocalisation phenotype than mys[8] males. The double mutant males have wild-type fertility.
The penetrance of the wing blistering phenotype seen in females expressing Fak56DScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4en-e16E at 25oC is increased from 28% to 44% if they are carrying mys8/+. Males expressing Fak56DScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4en-e16E at 25oC and hemizygous for mys8 show 31% wing blistering (compared to 0% wing blistering in a mys+ background).
The addition of wb4Y18/+ to mys8 hemizygotes enhances the wing blister phenotype, so that 28% of wings show a blistering phenotype. The addition of LanA9-32 does not enhance this phenotype.
The addition of crol04418, crol06470 does not enhance the wing blistering phenotype of mys8/Y flies. The addition of crolk08217 does enhance the wing blistering phenotype of mys8/Y flies by 3 fold. The addition of crol3ex5 enhances the wing blistering phenotype of mys8/Y flies by about 50%. This is significantly less than the enhancement seen by crolk08217. The addition of EcRC300Y or EcRM554fs enhances the wing blistering phenotype of mys8/Y flies by about three or four-fold respectively. The amount of balloon wings is increased several fold in both cases.
The frequency of wing blisters in mys8 hemizygotes is dominantly enhanced by bsV100, cassE48, puriV4 or moaV50. The frequency of wing blisters in mys8 hemizygotes is not dominantly enhanced by pygE16, pygE50, shotV104, shotV167, takV27, takV42, aukV106, aukV162, howT1, howT90, bsV121, cassE44, puriV15, bloV99, bloV134 or moaV88. The frequency of wing blisters seen in mys11/mys8 flies is not dominantly enhanced by potP3.
The frequency of wing blisters of mys8 hemizygotes is slightly increased (to approximately 20%) if the flies are also heterozygous for aukunspecified, auk2R-4, shotunspecified, shot65-2, blounspecified, blo2R-17, kiwiunspecified, kiwi2R-3, mamunspecified or 2R-L84-2, moderately increased (to approximately 40%) if the flies are also heterozygous for bub2L-2, and strongly increased if the flies are also heterozygous for bsunspecified, rhea2 or rhea1. The frequency of the wings held-out phenotype is enhanced if the flies are also heterozygous for stckunspecified, stck3R-17, rhea1, rhea2, pio2R-16 or piounspecified. Shows a weak genetic interaction with Dlunspecified.
Individuals in combination with cnomis1 exhibit incomplete lethality, few escapers have normal wings or an extra wing vein phenotype.
Enhances the blister but not vein phenotype of bs mutations.
Double mutants with mys8 have wing blister phenotype.
The held-out wing phenotype of mys8/mysG flies is dominantly enhanced by bsunspecified.
Double hemizygous and homozygous mutants with if3 display an extreme wing phenotype, wings are folded and unexpanded, veins and aberrant crossveins can be seen.
hide Xenogenetic Interactions
Statement
Reference
hide Complementation & Rescue Data
Fails to complement
Rescued by
Partially rescued by
mys8 is partially rescued by mys4A
mys8 is partially rescued by mys4B
mys8 is partially rescued by mysrDEA
mys8 is partially rescued by mysrFFA
Not rescued by
mys8 is not rescued by myst1
mys8 is not rescued by myst2
Comments
mys8 fails to complement the isoamyl acetate olfactory response phenotype seen in mysolfC-x3, mysolfC-x5, mysolfC-x10 and mysolfC-x17.
The if3/mys8 wing blister phenotype can be rescued by a mys+ transgene.
The held-out wing phenotype and blistered wing phenotype of males are rescued by mys4A, mys4B and mystZa.
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Discoverer
hide Comments
Double heterozygotes of LanA7-5/+ and mys8/+ indicated no interaction of the mutations.
Altered PSβ protein.
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hide Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym
mys8
 
nj42
 
Name Synonym
Secondary FlyBase IDs
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