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General Information
Symbol
Dmel\mysts1
Species
D. melanogaster
Name
FlyBase ID
FBal0012691
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

May contain regulatory mutation as lesion not found within exons or splice sites of mys.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

bouton & neuromuscular junction

bouton & neuromuscular junction & abdominal lateral longitudinal muscle 1

bouton & neuromuscular junction & abdominal ventral longitudinal muscle 1

bouton & neuromuscular junction & abdominal ventral longitudinal muscle 3

neuromuscular junction & abdominal lateral longitudinal muscle 1

neuromuscular junction & abdominal ventral longitudinal muscle 1

Detailed Description
Statement
Reference

mysts1 mutant third instar larvae show a synapse overgrowth phenotype with an increase in the number of synaptic boutons as well as immature undifferentiated 'ghost boutons' on the neuromuscular junctions. Unlike in wild-type, high K[+] stimulation in mysts1 mutants fails to induce a significant further increase in the number of ghost boutons and neither does it increase the bouton size. Similarly, the bouton size is also not increased upon high frequency (10Hz) nerve stimulation.

mysts1/mysts1 testes (at 25[o]C) show hub mislocalization (significant increase in distance from the tip to the hub compared to controls), though overall hub structure is not altered; at 29[o]C mysts1/mysts1 is semi-lethal but surviving mutant testes display similar 'pulling' hub phenotypes as seen in shv1 mutants.

Normal transverse tubule formation is seen in the abdominal temporary eclosion muscles of mys1/mysts1 pharate adults.

Homozygous and mysts1/mys8 flies show normal odour avoidance behaviour.

Mutant synapses at the larval neuromuscular junction show structural overgrowth, with an increased number of boutons in a highly branched, over-elaborated synaptic arbor.

Neuromuscular junctions (NMJs) of homozygous larvae are significantly larger and more complex than normal. This overgrowth phenotype is similar in animals raised at 25oC and 29oC, and is more obvious at the normally simpler muscle 4 terminal than the more complex muscle 12 terminal. The number of primary NMJ branches on muscle 4 is normal, but all higher order branching is very significantly increased, resulting in a highly significant increase in total branching. The number of type I boutons is significantly increased in the NMJs. The neuromuscular junctions of mysts1/mys11 larvae show no significant increase in bouton numbers. Homozygous and mysts1/mys11 larvae show altered synapse specificity at the muscle 12 NMJ. The NMJs may show a "backbranched" phenotype; at least one arboreal branch leaves muscle 12 and branches back onto muscle 13 from a site away from the point of nerve entry onto muscle 12. The NMJs may show a "pulled away" phenotype; boutons are suspended in the space between muscles 12 and 13 and defasciculation, or branching, occurs before the nerve has actually reached the point of insertion on the muscle. The NMJs may show a "multiple insertions" phenotype, where two or more separate and distinct nerve entry points can be seen on muscle 12. In these junctions, both nerves can frequently be traced back to a point of separation above muscle 13. Homozygous and mysts1/mys11 larvae have significantly larger nerve-evoked excitatory junctional current (EJC) amplitudes than normal at the muscle 4 NMJ. The frequency and mean (quantal) amplitude of miniature EJCs is normal at the NMJ in homozygous larvae.

Lethality occurs during the embryonic/larval stages.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

mysts1 is a suppressor of neuroanatomy defective phenotype of Fas2e86

Phenotype Manifest In
NOT Enhanced by
Statement
Reference

mysts1 has germinal proliferation center hub | heat sensitive phenotype, non-enhanceable by shv1/shv1

Suppressed by
NOT suppressed by
NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

mysts1 is a suppressor of bouton phenotype of Fas2e86

mysts1 is a suppressor of neuromuscular junction phenotype of Fas2e86

Additional Comments
Genetic Interactions
Statement
Reference

shv1;mysts1 double mutant third instar larvae display a synaptic overgrowth phenotype (increased number of boutons and immature, undifferentiated 'ghost boutons') that is comparable in severity to those observed in each of the mutants alone.

Expression of shvScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4nSyb.PS (but not Scer\GAL4how-24B) rescues the increased number of boutons on neuromuscular junctions characteristic for mysts1 mutant third instar larvae.

mysts1/mysts1; shv1/+ or mysts1/mysts1; shv1/shv1 testes (at 29[o]C) show similar hub mislocalization phenotypes (increased distance from tip to hub compared to controls) as either single mysts1/mysts1 or shv1/shv1 mutant alone.

Animals carrying Rat\CamKII-IAla.hs which are also mutant for mysts1 show an approximately 16% increase in bouton number at the neuromuscular junction. Expression of CaMKIIScer\UAS.cKa under the control of Scer\GAL4elav-C155 has no effect on the overgrowth phenotype seen at the mysts1 larval neuromuscular junction. Expression of CaMKIIScer\UAS.cKa under the control of Scer\GAL4Mhc.PW results in a slight, but insignificant, decrease in the overgrowth phenotype seen at the mysts1 larval neuromuscular junction, partially rescuing the overproduction of type I boutons. Expression of CaMKIIScer\UAS.cKa under the control of Scer\GAL4e22c completely rescues the overgrowth phenotype seen at the mysts1 larval neuromuscular junction. Bouton numbers at the larval neuromuscular junction are not significantly different from wild type in Fas2e86 mysts1 double mutants.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Not rescued by
Comments

Expression of mysScer\UAS.cUa driven by Scer\GAL4C833 rescues hub mislocalization in mysts1/mysts1 testes.

Fails to complement mys10, mysXN101, mysxR04, mys1 and mys11 at elevated temperatures.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Wright.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (15)