Mutant embryos show hypertrophy of the central and peripheral nervous systems.
Wing veins L3 and L5 thickened, thoracic microchaetae crowded and irregularly distributed.
N264-47 mutants exhibit elevated levels of asymmetry and reduced mean character size relative to Canton-S flies for thoracic bristles.
Hemizygous lethal. Heterozygotes have a neurogenic phenotype and have an increase in the number of thoracic bristles compared to wild-type.
Mutant embryos rarely form commissural or longitudinal connections. Although the first axons grow out at the same time as wild-type axons, they are never oriented towards the midline, and even fail to form connections on the ipsilateral side. Both anterior and middle pairs of midline glia are absent, and the posterior pair are duplicated but found at ectopic positions. There are 4-8 additional neuronal midline cells per segment.
Strong neurogenic phenotype.
N264-47 shows severe neural hypertrophy, a 6--9 fold increase in nau expressing cells per cluster relative to wild type. Mhc protein is also highly expressed and the Mhc expressing cells are arranged in irregular clusters.
Extreme embryonic neurogenic phenotype.
Typical Notch. Male embryos show developmental abnormalities like those of Df(1)N-8 (Poulson, 1939). Lethal with Nnd-3. N264-47/spl heterozygotes produce nonrecombinant N+ chromosomes with relatively high frequency (Welshons, 1958).