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General Information
Symbol
Dmel\N264-47
Species
D. melanogaster
Name
FlyBase ID
FBal0012750
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Mutagen
Nature of the Allele
Mutagen
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology

Polytene chromosomes apparently normal (Sutton).

Nature of the lesion
Statement
Reference

4bp deletion at nucleotide 11362 in EGF repeat 19, resulting in a TGA stop codon at codon 763.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mutant embryos show hypertrophy of the central and peripheral nervous systems.

Wing veins L3 and L5 thickened, thoracic microchaetae crowded and irregularly distributed.

N264-47 mutants exhibit elevated levels of asymmetry and reduced mean character size relative to Canton-S flies for thoracic bristles.

Hemizygous lethal. Heterozygotes have a neurogenic phenotype and have an increase in the number of thoracic bristles compared to wild-type.

Mutant embryos rarely form commissural or longitudinal connections. Although the first axons grow out at the same time as wild-type axons, they are never oriented towards the midline, and even fail to form connections on the ipsilateral side. Both anterior and middle pairs of midline glia are absent, and the posterior pair are duplicated but found at ectopic positions. There are 4-8 additional neuronal midline cells per segment.

Strong neurogenic phenotype.

N264-47 shows severe neural hypertrophy, a 6--9 fold increase in nau expressing cells per cluster relative to wild type. Mhc protein is also highly expressed and the Mhc expressing cells are arranged in irregular clusters.

Extreme embryonic neurogenic phenotype.

Lethality is not covered by Dp(1;3)N264-58.

Typical Notch. Male embryos show developmental abnormalities like those of Df(1)N-8 (Poulson, 1939). Lethal with Nnd-3. N264-47/spl heterozygotes produce nonrecombinant N+ chromosomes with relatively high frequency (Welshons, 1958).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

N264-47 has visible | dominant phenotype, enhanceable by Df(3R)Dl-FX3

Suppressed by
Statement
Reference

N264-47 has visible | dominant phenotype, suppressible by wgS107

Enhancer of
Statement
Reference

N264-47/N[+] is an enhancer of visible | dominant phenotype of Lsi

Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
Enhancer of
Statement
Reference

N264-47/N[+] is an enhancer of eye phenotype of Lsi

Additional Comments
Genetic Interactions
Statement
Reference

The severity of the eye phenotype of Lsi/+ flies is enhanced if they are also heterozygous for N264-47.

The number of thoracic bristles in N264-47 heterozygotes is increased further if the flies are also heterozygous for Df(3R)Dl-FX3, and the number of bristles is restored almost to wild-type in N264-47 ; wgS107 double heterozygotes.

Xenogenetic Interactions
Statement
Reference

Expression of N::Mmus\Notch1Nmf.Scer\UAS.T:Ecol\lexA under the control of Scer\GAL4da.G32 rescues the N264-47 neurogenic phenotype.

Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Demerec, June 1937.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (17)