|Feature type||allele||Associated gene||Dmel\N|
|Also Known As||Ax28, Ax1, Ax28a, NAx1, NAx, NAx28, Ax|
|Map ( GBrowse )|
|Allele class||antimorphic allele - genetic evidence, gain of function allele|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Amino acid replacement: N986I. N986 lies in EGF repeat 25. Has Gly at residue 2057 characteristic of Oregon R rather than Ser of Canton S (FBrf0045770).
Amino acid replacements in the EGF-homologous regions of the extracellular domain of the N protein.
A single-band duplication, presumed to be for 3C7 by Schultz (FBrf0005574). Lefevre et al., (FBrf0009019), on the other hand, argue against a duplication for 3C7 on the basis of equal X-ray-induced mutability to N in 'Ax' and +. Molecular information also incompatible with the presence of a duplication.
|Phenotype Manifest In|
When grown at 25oC, mutants lack the distal most parts of wing vein L5 band L4.
NAx-1 flies have mild eye-polarity defects; ommatidia are sometimes symmetrical, having an R4/R4-like phenotype.
Wing vein L2 is absent and wing vein L5 is interrupted.
NAx-1/NAx-16 larvae have enlarged wing discs compared to wild-type. This phenotype is associated with elevated mitotic activity in the disc, particularly in the peripheral region of the wing pouch.
The number of missing bristles increases with increasing temperature in homozygous flies, while the number and length of wing vein breaks increases from 18oC to 25oC and then decreases again from 25oC to 29oC.
Reduced number of bristles on the head and thorax and shortening of wing veins.
Homozygotes form wing vein gaps.
Thickened vein mutant.
Length of macrochaetae is reduced with respect to that of wild type. Suppresses the N haplo-insufficient phenotype of loss of the wing margin.
Neural precursors never form in the Ax class of N alleles. Cells mutant for Ax class alleles but with some neural potential are inhibited from becoming neural by their neighbours but do not themselves affect their neighbours, which can become neural.
Male viable at 25oC but nearly lethal at 29oC. NAx-1/NAx-E2 semi-lethal at 25oC and lethal at 29oC. NAx-1/+ females show short L5 in half of the flies and sparse hair pattern on thorax. Lower temperature (19oC) markedly decreases expression, and higher temperature enhances it. Some Ax alleles enhance N expression in NAx-1/N heterozygotes, but others suppress the dominant N phenotype. For example, NAx-1/Df(1)N-8 approaches wild type in all characteristics. No wing-vein interruption in NAx-1/+ at 18oC and 26oC and enhancement by H1 occurs. NAx-1/NAx-1; ciD/+ and NAx-1/Y; ciD/+ are lethal or nearly so at 26oC. At 22oC, males survive and show enhanced wing-vein interruption and more missing bristles. Wing nicking is suppressed in NAx-1/N55e11 at 25oC and Ax venation is weakly expressed. In heterozygotes of NAx-1 with the recessives at N at 18oC and 25oC, there is neither expression of the recessive nor Ax-type venation. Temperature-sensitive period for lethality of NAx-1 at beginning of pupal stage; of NAx-1/NAx-E2 at end of third instar and into early pupal stage.
Phenotypes have topographic pattern specificities.
Wing phenotype is enhanced by mutations at da.
Some hemizygous males occasionally lack the twin sensilla of the wing margin, the ventral sensillum of wing vein L3, and the anterior cross vein sensillum.
The L5 wing vein phenotype is more severe at 25oC than 20oC, the temperature-sensitive period is between 10 and 15% of pupal development time.
Nearly lethal when reared at 29oC; temperature-sensitive period early pupa.
|NOT Enhancer of|
|NOT Suppressor of|
|Phenotype Manifest In|
|NOT Enhanced by|
|NOT suppressed by|
Expression of p38bDN.Scer\UAS under the control of Scer\GAL4unspecified has no significant effect on the wing phenotype caused by NAx-1.
NAx-1 suppresses the supernumerary scutellar bristle phenotype seen in flies with m4Scer\UAS.cAa driven by Scer\GAL4455.2. The addition of NAx-1 reduces the number of supernumerary scutellar bristles seen in flies with mαScer\UAS.cAa driven by Scer\GAL4455.2, though there are still more bristles seen than in wild-type.
The variation of the number of missing bristles and number and length of wing vein breaks with temperature in NAx-1; E(spl)1 double mutants is the same as in E(spl)1 single mutants.
dpps4/dpps8 NAx-1 or tkv1/tkv8 NAx-1 double mutant flies show pronounced wing vein loss. The thickening of veins seen in tkv1/tkv8 flies is suppressed in combination with NAx-1.
|Complementation & Rescue Data|
|Fails to complement|
|Stocks ( 0 )|
|Notes on Origin|
Nazarenko, Jan. 1928.
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 11 )|
(de Celis and Bray, 2000, Grushko et al., 1998, Royet et al., 1998, Grushko et al., 1998, de Celis et al., 1997, de Celis, 1997, Gomez-Skarmeta and Modolell, 1996, de Celis et al., 1996, de Celis et al., 1995, de Celis et al., 1991, de Celis and Garcia-Bellido, 1994, de Celis et al., 1993, Heitzler and Simpson, 1993, )
|Secondary FlyBase IDs|
|References ( 43 )|
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|Recent research papers (0)|
|All research papers listed in FlyBase were published before 2011|