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General Information
Symbol
Dmel\NAx-16
Species
D. melanogaster
Name
FlyBase ID
FBal0012852
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Ax16172, NAx16, Ax16
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology

Polytene chromosomes are normal (Foster, 1975).

Nature of the lesion
Statement
Reference

Amino acid replacement in the EGF-homologous regions of the extracellular domain of the N protein.

Amino acid replacement: G1174A. Mutant partially sequenced; correlated with single amino acid replacements within six adjacent EGF-homologous elements of the N protein; has Gly at residue 2057 characteristic of Oregon R rather than Ser of Canton S (Kelley, Kidd, Deutsch and Young, 1987). Mutation involves a change from glycine to alanine in residue 1174 in EGF-like repeat 29; GGA --> AGA (Kelley, Kidd, Deutsch and Young, 1987).

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

NAx-16 heterozygous adults display a significant decrease in the number of thoracic sensory bristles, as compared to controls.

NAx-16 heterozygous adult females display wing vein defects: shortening of the longitudinal L5 vein.

Heterozygotes have show truncation of the L4 and L5 wing veins.

Mutant flies show loss of wing vein.

Hemizygous males show shortening of the L4 and L5 wing veins.

The average size of clones induced in NAx-M3/NAx-16 mutant wing discs are larger all over the wing than in wild-type controls. Clones of over 2000 cells can be seen in the mutant discs (compared to a maximum of 600 in controls). This suggests that proliferation rate in NAx-M3/NAx-16 mutant wings is higher than in controls.

Flies show missing macrochaetae and reduced density of microchaetae. The fifth wing vein is shortened.

NAx-1/NAx-16 larvae have enlarged wing discs compared to wild-type. This phenotype is associated with elevated mitotic activity in the disc, particularly in the peripheral region of the wing pouch.

Viable in combination with NAx-71d or NAx-E2. NAx-1/NAx-16 females have reduced viability. This viability is further reduced if the flies also carry Dp(3;3)MKRS-D2 or Dp(3;3)bxd110. Lethal in combination with NAx-9; unescaped female pupae show a strong antineurogenic phenotype.

Wing veins are missing.

Flies show shortening of the wing veins.

Length of microchaetae, macrochaetae and longitudinal wing veins is reduced with respect to that of wild type. Enhances the N haplo-insufficient phenotype of loss of the wing margin.

Lack of chaetae. NAx-1 and NAx-16 correct Hw phenotypes in both ectopic and normal positions.

Viable, enhancer type.

Heterozygous females raised at 29oC have significantly fewer ocellar bristles and more gaps in the longitudinal wing veins than heterozygous females raised at 22oC. The temperature sensitive period for both phenotypes occurs during the third larval instar stage. NAx-16/N264-40 females show temperature sensitive lethality. The temperature sensitive period for lethality is during the second larval instar stage.

Homozygotes resemble NAx-1. NAx-16 is less fertile than alleles NAx-E2, NAx-71d and NAx-9 (Portin, 1975) and temperature-sensitive for the bristle and wing effects of Ax (Foster, 1975). In heterozygotes with Notch, Ax is expressed and the Notch wing effect is enhanced (Foster, 1975; Portin, 1975). At 29oC, heterozygotes with N are lethal. In NAx-16/N264-40 heterozygotes, the TSP for lethality is in the second instar and for Ax-morphological effects, it is in the third instar (Foster, 1973; Foster, 1975). In heterozygotes with recessive visibles at Notch, all are complementary at 18oC and 25oC; at 29oC, there are mild indications of noncomplementarity with nd and Nnd-2 (Portin, 1977). Heteroalleles NAx-16/NAx-E2 and NAx-16/NAx-71d are viable (Foster, 1975; Portin, 1975); NAx-16/NAx-E1 is inviable (negative heterosis) and heterozygotes with NAx-9 and NAx-1 are lethal (negative complementation) (Foster, 1975; Portin, 1975), but Dp(1;2)51b restores viability (Portin, 1977). Heterozygotes with the lethal alleles NAx-59b and NAx-59d are lethal and mostly inviable upon the addition of Dp(1;2)51b (Portin, 1975; Portin, 1977). On the genetic map of Notch, probably between N264-40 and NCo based on the failure to obtain recombinants between NAx-16 and NAx-9 (Foster, 1975).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

NAx-16 has visible | dominant phenotype, enhanceable by lid[+]/Kdm510424

NAx-16 has visible phenotype, enhanceable by f06222f06222/f06222[+]

NAx-16 has visible phenotype, enhanceable by klud00059/klu[+]

NAx-16 has visible phenotype, enhanceable by GPHRc06331/CG8090[+]

Suppressed by
Statement
Reference

NAx-16 has visible | adult stage phenotype, suppressible by Kap-α3D93

NAx-16 has visible | adult stage phenotype, suppressible by Kap-α3D165

NAx-16 has visible | adult stage phenotype, suppressible by Kap-α3S033513

NAx-16 has visible | dominant phenotype, suppressible by Su(var)3-3[+]/Su(var)3-3ΔN

NAx-16 has visible phenotype, suppressible | partially by sca[+]/scad09400

NAx-16 has visible phenotype, suppressible by A122A122

NAx-16 has visible phenotype, suppressible | partially by kuz1405Rev11

Suppressor of
Statement
Reference

N+/NAx-16 is a suppressor | partially of visible | heat sensitive phenotype of Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

NAx-16 has wing vein phenotype, enhanceable by lid[+]/Kdm510424

NAx-16 has wing vein L4 phenotype, enhanceable by klud00059/klu[+]

NAx-16 has wing vein L5 phenotype, enhanceable by klud00059/klu[+]

NAx-16 has wing vein L4 phenotype, enhanceable by GPHRc06331/CG8090[+]

NAx-16 has wing vein L5 phenotype, enhanceable by GPHRc06331/CG8090[+]

NAx-16 has wing vein L4 phenotype, enhanceable by f06222f06222/f06222[+]

NAx-16 has wing vein L5 phenotype, enhanceable by f06222f06222/f06222[+]

Suppressed by
Statement
Reference

NAx-16 has wing vein L5 phenotype, suppressible by Kap-α3D93

NAx-16 has wing vein L5 phenotype, suppressible by Kap-α3D165

NAx-16 has wing vein L5 phenotype, suppressible by Kap-α3S033513

NAx-16 has wing vein phenotype, suppressible by Su(var)3-3[+]/Su(var)3-3ΔN

NAx-16 has wing vein L4 phenotype, suppressible by sca[+]/scad09400

NAx-16 has phenotype, suppressible by bs03267

NAx-16 has wing vein phenotype, suppressible | partially by kuz1405Rev11

NOT suppressed by
Statement
Reference

NAx-16 has wing vein L5 phenotype, non-suppressible by sca[+]/scad09400

Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The decreased number of thoracic sensory bristles present in NAx-16 heterozygous adults is suppressed by the expression of Rbfox1dsRNA.Scer\UAS under the control of Scer\GAL4sca-109-68.

The wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa is dominantly partially suppressed if the flies are also heterozygous for NAx-16.

The shortened longitudinal L5 phenotype characteristic for NAx-16 heterozygous females is suppressed by combination with a single copy of any of the following alleles: Kap-α3D93, Kap-α3D165 or Kap-α3S033513.

The wing vein truncation phenotype which is seen in NAx-16/+ flies is suppressed by Su(var)3-3ΔN/+.

The wing vein truncation phenotype which is seen in NAx-16/+ flies is enhanced by lid10424/+.

scad09400 suppresses the wing vein L4 but not the wing vein L5 phenotypes of NAx-16 hemizygous males.

klud00059, CG8090c06331 or f06222f06222 enhance the L4 and L5 wing vein shortening phenotypes of NAx-16 hemizygous males.

Homozygous viability is decreased if the flies also carry Dp(3;3)MKRS-D2 or Dp(3;3)bxd110. The antineurogenic phenotype of NAx-16/NAx-9 female escapers is enhanced in the presence of Dp(3;3)MKRS-D2 or Dp(3;3)bxd110.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

Lewis & Bacher.

Comments
Comments

NAx-16 shows negative complementation with NAx-M1.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (27)