|Feature type||allele||Associated gene||Dmel\N|
|Also Known As||Ax16172, Ax16|
|Allele class||antimorphic allele - genetic evidence, gain of function allele|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Amino acid replacement in the EGF-homologous regions of the extracellular domain of the N protein.
Amino acid replacement: G1174A. Mutant partially sequenced; correlated with single amino acid replacements within six adjacent EGF-homologous elements of the N protein; has Gly at residue 2057 characteristic of Oregon R rather than Ser of Canton S (Kelley, Kidd, Deutsch and Young, 1987). Mutation involves a change from glycine to alanine in residue 1174 in EGF-like repeat 29; GGA --> AGA (Kelley, Kidd, Deutsch and Young, 1987).
Polytene chromosomes are normal (Foster, 1975).
|Phenotype Manifest In|
Heterozygotes have show truncation of the L4 and L5 wing veins.
Mutant flies show loss of wing vein.
Hemizygous males show shortening of the L4 and L5 wing veins.
The average size of clones induced in NAx-M3/NAx-16 mutant wing discs are larger all over the wing than in wild-type controls. Clones of over 2000 cells can be seen in the mutant discs (compared to a maximum of 600 in controls). This suggests that proliferation rate in NAx-M3/NAx-16 mutant wings is higher than in controls.
NAx-1/NAx-16 larvae have enlarged wing discs compared to wild-type. This phenotype is associated with elevated mitotic activity in the disc, particularly in the peripheral region of the wing pouch.
Flies show missing macrochaetae and reduced density of microchaetae. The fifth wing vein is shortened.
Viable in combination with NAx-71d or NAx-E2. NAx-1/NAx-16 females have reduced viability. This viability is further reduced if the flies also carry Dp(3;3)MKRS-D2 or Dp(3;3)bxd110. Lethal in combination with NAx-9; unescaped female pupae show a strong antineurogenic phenotype.
Wing veins are missing.
Flies show shortening of the wing veins.
Length of microchaetae, macrochaetae and longitudinal wing veins is reduced with respect to that of wild type. Enhances the N haplo-insufficient phenotype of loss of the wing margin.
Viable, enhancer type.
Heterozygous females raised at 29oC have significantly fewer ocellar bristles and more gaps in the longitudinal wing veins than heterozygous females raised at 22oC. The temperature sensitive period for both phenotypes occurs during the third larval instar stage. NAx-16/N264-40 females show temperature sensitive lethality. The temperature sensitive period for lethality is during the second larval instar stage.
Homozygotes resemble NAx-1. NAx-16 is less fertile than alleles NAx-E2, NAx-71d and NAx-9 (Portin, 1975) and temperature-sensitive for the bristle and wing effects of Ax (Foster, 1975). In heterozygotes with Notch, Ax is expressed and the Notch wing effect is enhanced (Foster, 1975; Portin, 1975). At 29oC, heterozygotes with N are lethal. In NAx-16/N264-40 heterozygotes, the TSP for lethality is in the second instar and for Ax-morphological effects, it is in the third instar (Foster, 1973; Foster, 1975). In heterozygotes with recessive visibles at Notch, all are complementary at 18oC and 25oC; at 29oC, there are mild indications of noncomplementarity with nd and Nnd-2 (Portin, 1977). Heteroalleles NAx-16/NAx-E2 and NAx-16/NAx-71d are viable (Foster, 1975; Portin, 1975); NAx-16/NAx-E1 is inviable (negative heterosis) and heterozygotes with NAx-9 and NAx-1 are lethal (negative complementation) (Foster, 1975; Portin, 1975), but Dp(1;2)51b restores viability (Portin, 1977). Heterozygotes with the lethal alleles NAx-59b and NAx-59d are lethal and mostly inviable upon the addition of Dp(1;2)51b (Portin, 1975; Portin, 1977). On the genetic map of Notch, probably between N264-40 and NCo based on the failure to obtain recombinants between NAx-16 and NAx-9 (Foster, 1975).
|Phenotype Manifest In|
|NOT suppressed by|
The wing vein truncation phenotype which is seen in N[Ax-16]/+ flies is suppressed by Su(var)3-3[ΔN]/+. The wing vein truncation phenotype which is seen in N[Ax-16]/+ flies is enhanced by lid/+.
sca[d09400] suppresses the wing vein L4 but not the wing vein L5 phenotypes of N[Ax-16] hemizygous males. klu[d00059], CG8090[c06331] or f06222[f06222] enhance the L4 and L5 wing vein shortening phenotypes of N[Ax-16] hemizygous males.
|Complementation & Rescue Data|
|Fails to complement|
|Stocks ( 0 )|
|Notes on Origin|
Lewis & Bacher.
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 4 )|
(Garoia et al., 2000, Baonza et al., 2000, Baonza and Garcia-Bellido, 2000, de Celis and Bray, 2000, Roch et al., 1998, Grushko et al., 1998, Sotillos et al., 1997, de Celis et al., 1997, Gomez-Skarmeta and Modolell, 1996, de Celis et al., 1996, de Celis et al., 1991, de Celis and Garcia-Bellido, 1994, de Celis et al., 1993, Xu et al., 1990, Foster, 1973, Egoz-Matia et al., 2011)
|Secondary FlyBase IDs|
|References ( 24 )|
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|Recent research papers ( 2 )|