Allele Dmel\N^fa-swb

General Information
Symbol	Dmel\N^fa-swb	Species	D. melanogaster
Name	facet-strawberry	FlyBase ID	FBal0012874
Feature type	allele	Created / Updated	2006-08-22/2006-08-22
Associated gene	Dmel\N

Allele class
Mutagen	X ray
Nature of the Allele
Allele class
Mutagen	X ray
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference 0.8 kb deletion in -28.4 to -27.1 Caused by a deficiency of 800 base pairs in the restriction fragment -- 26.2 to -- 25.0 (Grimwade, Muskavitch, Welshons, Yedvobnick and Artavanis-Tsakonas, 1985; this is 67 base pairs upstream from the start of transcription of Notch. Deletes the 3' end of the locus immediately to the left of N (Kidd, Kelley and Young, 1986). 0.8kb deletion between coordinates -27.3 and -26.0 (zero coordinate is defined as the centre of the 2.2kb EcoRI fragment that encompasses the N^76b8 inversion breakpoint). (Grimwade et al., 1985) Deletion of 0.8kb DNA from the 5' end of the N locus. (Welshons and Welshons, 1986) A complex event involving 2 deletions that result in a 880bp deletion at the distal end of N. (Ramos et al., 1989) 800bp deletion removing the 3' part of the gene immediately upstream of N and almost all of the sequences up to the N transcription start site. (Gorman and Girton, 1992) Small deletion 5' of N. (Reiter et al., 1996)
Assay mode
Caused by aberration	Df(1)fa-swb (Welshons and Welshons, 1986)
Cytology	Deficiency for interband between 3C6 and 7, causing these bands to fuse (Keppy and Welshons, 1977).
Phenotypic Data
Phenotypic Class
	visible (Rabinow and Birchler, 1990) visible \| recessive (Ramos et al., 1989, Gorman and Girton, 1992, Welshons and Welshons, 1986)
Phenotype Manifest In
	eye (Ramos et al., 1989, Rabinow and Birchler, 1990, Welshons and Welshons, 1986) primary pigment cell (Reiter et al., 1996)
Detailed Description
	Statement Reference In males, eyes are rough with a variable tendency to be glossy; with N^fa-g and N^fa-g62, eyes are very rough, but mutant condition is not as extreme as that found in homozygous glossy-eyed mutants. In heterozygotes with N^fa-1, eyes are slightly rough, overlapping wild type; with spl, the eyes are wild type. The N^fa-swb allele, like N^fa-1, is not dosage compensated and the mutant condition is poorly expressed in females. N^fa-swb/N^nd-3 has slight deltas at junction of longitudinal veins with marginal veins; in heterozygotes of N^fa-swb, N^55e11 and N^264-40, the eyes are glossy and the Notch phenotype is enhanced, resulting in reduced viability and fertility; with the temperature-sensitive N^60g11, heterozygotes are less mutant, viable, and fertile. In double mutants, N^fa-swb N^fa-g, the males have N^fa-g-like eyes; and wing veins are thickened and δ like at tips; they resemble N^nd-3 males except that wings are seldom notched. The wing-venation effect is less extreme in homozygous females (Welshons and Keppy, 1975; Keppy and Welshons, 1977). Removal of the region of 3C2-5 from the vicinity of N^fa-swb, either by deletion or inversion results in suppression of N^fa-swb; also affected by spontaneously arising, cis-acting enhancer [e(fa^swb)] and suppressor [su(fa^swb)] mutations, both of which map to the 3C2-5 region. (Welshons and Welshons, 1986). Phenotype is similar to N^fa-g; flies have rough, glossy eyes. The eye phenotype is less mutant in homozygous females than hemizygous males. N^fa-swb/N^fa-g flies have rough eyes that are not glossy. (Welshons and Welshons, 1986) Homozygotes exhibit rough eyes. (Rabinow and Birchler, 1990) Pronounced eye phenotype. Does not interact with dx^ENU. (Xu and Artavanis-Tsakonas, 1990) Interacts with Df(1)rst2 to suppress eye roughening. (Wolff and Ready, 1991) Disrupted transverse rows of bristles on the first tarsal segments, few extra sex cob bristles and rough eyes. (Gorman and Girton, 1992) Differentiation of primary pigment cells varies between ommatidia. (Reiter et al., 1996)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference N^fa-swb has eye phenotype, enhanceable by e(fa^swb)¹ (Welshons and Welshons, 1986)
Suppressed by
Statement Reference N^fa-swb has phenotype, suppressible by In(1)78b (Welshons and Welshons, 1986) N^fa-swb has phenotype, suppressible by su(fa^swb)¹ (Welshons and Welshons, 1986)
Other
Statement Reference N^fa-swb, e(fa^swb)¹ has metathoracic tibia phenotype (Welshons and Welshons, 1986, Welshons and Welshons, 1986) N^fa-swb, e(fa^swb)¹ has microchaeta phenotype (Welshons and Welshons, 1986, Welshons and Welshons, 1986)
Additional Comments
Genetic Interactions
Statement Reference The phenotype is enhanced by e(fa^swb)¹; e(fa^swb)¹ in cis with N^fa-swb in either the homozygous or hemizygous condition produces flies with darkly pigmented, very rough, slightly narrow eyes. In addition there is a proliferation of microchaetae over the thorax, abdomen and legs, and the tibiae of the rear legs are distinctly bowed. The phenotype is suppressed by su(fa^swb)¹ in cis with N^fa-swb in either the homozygous or hemizygous condition. The phenotype is also suppressed by In(1)78b. (Welshons and Welshons, 1986) Synergistic interactions with dx alleles. (Gorman and Girton, 1992)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	N^fa-swb is rescued by N^+tCos479 (Ramos et al., 1989)
Not rescued by	N^fa-swb is not rescued by N^mMGIIa (Ramos et al., 1989)
Comments	N^fa-swb complements nd and N^nd-2. N^+tCos479 rescues the mutant phenotype but N^mMGIIa construct only partially rescues. (Ramos et al., 1989)
Stocks ( 1 )
Bloomington	1715 w^a N^fa-swb
Notes on Origin
Discoverer	Lefevre and Kelley.

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	fa^swb (West et al., 2002, Vazquez and Schedl, 2000, Reiter et al., 1996, Gorman and Girton, 1992, Wolff and Ready, 1991, Xu and Artavanis-Tsakonas, 1990, Rabinow and Birchler, 1990, Ramos et al., 1989, Welshons and Welshons, 1986, Grimwade et al., 1985, Artavanis-Tsakonas et al., 1983, ) facet^strawberry (Dickson and Hafen, 1993) N^fa-swb swb^71b
Name Synonym	facet-strawberry
Secondary FlyBase IDs

References ( 16 )
Research paper	Vazquez and Schedl, 2000, Genetics 155(3): 1297--1311 Deletion of an insulator element by the mutation facet-strawberry in Drosophila melanogaster. [FBrf0128672] Reiter et al., 1996, Development 122(6): 1931--1940 Reorganization of membrane contacts prior to apoptosis in the Drosophila retina: the role of the IrreC-rst protein. [FBrf0088828] Gorman and Girton, 1992, Genetics 131: 99--112 A genetic analysis of deltex and its interaction with the Notch locus in Drosophila melanogaster. [FBrf0056273] Wolff and Ready, 1991, Development 113(3): 825--839 Cell death in normal and rough eye mutants of Drosophila. [FBrf0053823] Rabinow and Birchler, 1990, Genetics 125: 41--50 Interactions of vestigial and scabrous with the Notch locus of Drosophila melanogaster. [FBrf0051947] Xu and Artavanis-Tsakonas, 1990, Genetics 126: 665--677 deltex, a locus interacting with the neurogenic genes, Notch, Delta and mastermind in Drosophila melanogaster. [FBrf0051969] Ramos et al., 1989, Genetics 123: 337--348 Physical and functional definition of the Drosophila Notch locus by P-element transformation. [FBrf0049895] Welshons and Welshons, 1986, Genetics 113(2): 337--354 Enhancement and suppression of a euchromatic position effect at Notch in Drosophila melanogaster. [FBrf0044443] Grimwade et al., 1985, Dev. Biol. 107: 503--519 The molecular genetics of the Notch locus in Drosophila melanogaster. [FBrf0042381] Artavanis-Tsakonas et al., 1983, Proc. Natl. Acad. Sci. USA 80: 1977--1981 Molecular cloning of Notch, a locus affecting neurogenesis in Drosophila melanogaster. [FBrf0040118] Keppy and Welshons, 1977, Genetics 85: 497--506 The cytogenetics of a recessive visible mutant associated with a deficiency adjacent to the Notch locus in Drosophila melanogaster. [FBrf0030152] Welshons and Keppy, 1975, Genetics 80: 143--155 Intragenic deletions and salivary band relationships in Drosophila. [FBrf0027479] Lefevre and Kelley, 1972, D. I. S. 48: 146--147 Strawberry vs. facet-glossy, a locus correction. [FBrf0023597]
Review	West et al., 2002, Genes Dev. 16(3): 271--288 Insulators: many functions, many mechanisms. [FBrf0146762] Bell et al., 2001, Science 291(5503): 447--450 Insulators and boundaries: versatile regulatory elements in the eukaryotic genome. [FBrf0134841] Dickson and Hafen, 1993, Bate, Martinez Arias, 1993: 1327--1362 Genetic dissection of eye development in Drosophila. [FBrf0064799]

Allele Dmel\Nfa-swb

Allele Dmel\N^fa-swb