Allele Dmel\N^l1N-ts2

General Information
Symbol	Dmel\N^l1N-ts2	Species	D. melanogaster
Name		FlyBase ID	FBal0012888
Feature type	allele	Associated gene	Dmel\N
Also Known As	N^ts2

Allele class	heat sensitive hypomorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	heat sensitive hypomorphic allele - genetic evidence (Shellenbarger and Mohler, 1975)
Mutagen	ethyl methanesulfonate (Shellenbarger and Mohler, 1975)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage \| heat sensitive (Ge et al., 2004) lethal \| embryonic stage \| neurogenic phenotype \| recessive (Lehmann et al., 1983) lethal \| recessive \| heat sensitive (Shellenbarger and Mohler, 1975) memory defective \| heat sensitive (Ge et al., 2004) visible \| heat sensitive (Glise et al., 2002)
Phenotype Manifest In
	adult head \| heat sensitive (Shellenbarger and Mohler, 1975) border follicle cell \| heat sensitive (Wang et al., 2007) embryonic epidermis (Lehmann et al., 1983) eye \| heat sensitive (Shellenbarger and Mohler, 1975) eye-antennal disc (Kenyon et al., 2003) leg \| heat sensitive (Shellenbarger and Mohler, 1975) macrochaeta \| heat sensitive (Shellenbarger and Mohler, 1975) microchaeta \| heat sensitive (Shellenbarger and Mohler, 1975) ommatidium \| heat sensitive (Shellenbarger and Mohler, 1975) wing \| distal \| heat sensitive (Glise et al., 2002) wing disc \| heat sensitive (Glise et al., 2002) wing vein L3 \| heat sensitive (Crozatier et al., 2003)
Detailed Description
	Statement Reference Wound healing does not appear to be affected compared to controls in N[l1N-ts2]/+ wing discs which have been fragmented (a 90[o] sector has been dissected out of the posterior compartment, leaving a 3/4 anterior fragment) and then implanted into wild-type females (analysed at 24 and 48 hours after implantation). However, the N[l1N-ts2]/+ regenerating discs show significantly impaired proliferation compared to controls. (Blanco et al., 2010) Stage 10 N[l1N-ts2] homozygous females, that have been shifted to a non-permissive temperature for less than 6 hours exhibit migration defects, despite these egg chambers already developing to stage 9, and follicle cell differentiation occurring, prior to the temperature shift (whereas wild-type controls exhibited no migration defects). (Wang et al., 2007) Adult flies show normal reactivity to electric shock and normal olfactory acuity (to methylcyclohexanol and octanol) after incubation at the restrictive temperature (30^oC) for 2 days. Adult flies incubated for 2 days at the restrictive temperature show normal learning scores in a Pavlovian learning and memory assay. However, these flies show a significant reduction in one-day memory after spaced training compared to flies maintained at the permissive temperature. (Ge et al., 2004) When N^l1N-ts2 mutants are shifted to restrictive temperature between 104 and 128 h after egg laying (AEL) (8-32h after puparium formation (APF)), the L3 vein is broader, indicative of abnormal vein resolution. The campaniform sensillae (CS) are centered over the broader vein. When a transient temperature shift is applied at 60-80 h AEL, the L3 vein retains a wild-type width but its position is shifted anteriorly relative to the CS. (Crozatier et al., 2003) Shifting N^l1N-ts2 mutant larvae to the nonpermissive temperature in mid-late-L1 results in eye-antennal discs that are reduced in size. (Kenyon et al., 2003) Mutant larvae shifted to the non-permissive temperature (29^oC) at the beginning of the L2 stage have abnormal wing discs. Mutant larvae shifted to the non-permissive temperature (29^oC) at the beginning of the L3 stage have wing discs with a smaller wing pouch than normal. Adults derived from these larvae have notches at the distal tip of the wing. (Glise et al., 2002) Weak embryonic neurogenic phenotype at 29^oC. (Lehmann et al., 1983) Lethal in combination with Df(1)N-8 at both 18^oC and 29^oC. 8% of homozygotes survive at 29^oC, 97% survive at 18^oC. Wings are normal in heterozygotes. Homozygotes are morphologically normal at 18^oC. At 29^oC, late pupae are head-eyeless, have a rough eye, stubby legs and irregular chaetae. Lethal in combination with N^l1N-ts1 at 29^oC. 40% of N^l1N-ts2/N^Ax-tsl flies survive at 29^oC and have an abruptex phenotype. (Shellenbarger and Mohler, 1975) Similar to N^l1N-ts1 except for occasional survival of homozygotes to the pupal stage at 29^oC and weaker expression of recessive visible defects in heterozygotes at this temperature.
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Complements	N^fa-1 (Shellenbarger and Mohler, 1975) N^fa-g (Shellenbarger and Mohler, 1975) N^nd-1 (Shellenbarger and Mohler, 1975) N^nd-2 (Shellenbarger and Mohler, 1975) N^nd-3 (Shellenbarger and Mohler, 1975) N^spl-1 (Shellenbarger and Mohler, 1975)
Fails to complement	N^fa-1 (Shellenbarger and Mohler, 1975) N^fa-g (Shellenbarger and Mohler, 1975) N^nd-1 (Shellenbarger and Mohler, 1975) N^nd-2 (Shellenbarger and Mohler, 1975) N^nd-3 (Shellenbarger and Mohler, 1975) N^spl-1 (Shellenbarger and Mohler, 1975)
Comments	Failure to complement is at 29^oC, complementation is at 18^oC. (Shellenbarger and Mohler, 1975)
Stocks ( 2 )
Bloomington	3075 w^a N^l1N-ts2 rb¹
Kyoto	106821 w^a N^l1N-ts2 rb¹
Notes on Origin
Discoverer	Shellenbarger.

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	l(1)N^ts2 (Shellenbarger and Mohler, 1975, ) N^11N-ts2 (Kenyon et al., 2003) N^I1N-ts2 (Blanco et al., 2010) N^l1N-ts2 N^ts2 (Ge et al., 2004, Crozatier et al., 2003, Glise et al., 2002, Lehmann et al., 1983, Wang et al., 2007, Scherfer et al., 2008, Herranz et al., 2006) N^ts (Lee and Adler, 2004) Notch^ts2 (Krzemień et al., 2007) Notch^ts (Krzemień et al., 2007)
Name Synonym
Secondary FlyBase IDs

References ( 13 )
Research paper	Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Scherfer et al., 2008, Dev. Biol. 323(2): 189--196 Drosophila Serpin-28D regulates hemolymph phenoloxidase activity and adult pigmentation. [FBrf0207215] Krzemień et al., 2007, Nature 446(7133): 325--328 Control of blood cell homeostasis in Drosophila larvae by the posterior signalling centre. [FBrf0194113] Wang et al., 2007, Dev. Biol. 301(2): 532--540 Spatially localized Kuzbanian required for specific activation of Notch during border cell migration. [FBrf0191839] Herranz et al., 2006, EMBO Rep. 7(3): 297--302 Self-refinement of Notch activity through the transmembrane protein Crumbs: modulation of gamma-Secretase activity. [FBrf0190373] Ge et al., 2004, Proc. Natl. Acad. Sci. U.S.A. 101(27): 10172--10176 Notch signaling in Drosophila long-term memory formation. [FBrf0179219] Lee and Adler, 2004, Mech. Dev. 121(1): 37--49 The grainy head transcription factor is essential for the function of the frizzled pathway in the Drosophila wing. [FBrf0167844] Crozatier et al., 2003, Mech. Dev. 120(5): 529--535 Vein-positioning in the Drosophila wing in response to Hh; new roles of Notch signaling. [FBrf0160464] Kenyon et al., 2003, Dev. Cell 5(3): 403--414 Coordinating proliferation and tissue specification to promote regional identity in the Drosophila head. [FBrf0162063] Glise et al., 2002, Dev. Biol. 248(1): 93--106 Notch and wingless modulate the response of cells to hedgehog signalling in the Drosophila wing. [FBrf0151253] Lehmann et al., 1983, Rouxs Arch. Dev. Biol. 192(2): 62--74 On the phenotype and development of mutants of early neurogenesis in Drosophila melanogaster. [FBrf0040185] Shellenbarger and Mohler, 1975, Genetics 81: 143--162 Temperature-sensitive mutations of the Notch locus in Drosophila melanogaster. [FBrf0027480] Shellenbarger, 1972, D. I. S. 48: 55 Evidence that EMS induces point mutations at high frequency. [FBrf0023652]

Allele Dmel\Nl1N-ts2

Allele Dmel\N^l1N-ts2