Allele Dmel\ncd²

General Information
Symbol	Dmel\ncd²	Species	D. melanogaster
Name		FlyBase ID	FBal0012911
Feature type	allele	Associated gene	Dmel\ncd
Also Known As	ncd

Map ( GBrowse )
Allele class	loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	loss of function allele (Hatsumi and Endow, 1992, Komma and Endow, 1997)
Mutagen	ethyl methanesulfonate (Yamamoto et al., 1989, Hatsumi and Endow, 1992, Endow and Komma, 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3R:25,630,895..25,630,895 comment=site of nucleic acid difference inferred by FlyBase based on stated amino acid change evidence=experimental na_change=G25630895C pr_change=G446R\|ncd-PA reported_pr_change=G446R (Endow and Komma, 1997)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: G446R. Amino acid replacement: A566S. Amino acid replacement: G696A. G446R change affects a highly conserved glycine residue in the ATP-binding region, A566S is a conservative amino acid change and G696A is outside the motor domain. (Endow and Komma, 1997) no 2.6 kb deletion
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage \| partially \| recessive (Endow and Komma, 1998) lethal \| partially \| semidominant (Endow and Komma, 1997) meiotic cell cycle defective (Hatsumi and Endow, 1992) meiotic cell cycle defective \| recessive (Yamamoto et al., 1989, Endow and Komma, 1997) mitotic cell cycle defective \| recessive (Endow and Komma, 1997)
Phenotype Manifest In
	meiosis & nuclear chromosome & oocyte (Hatsumi and Endow, 1992)
Detailed Description
	Statement Reference Most of the meiotic exceptions result from chromosome loss. Frayed and monopolar spindles are frequently observed. (Sekelsky et al., 1999) Approximately 90% of eggs laid by homozygous females fail to hatch. (Endow and Komma, 1998) Homozygous females show increased chromosome nondisjunction and loss compared to wild-type. Embryos derived from homozygous females have reduced viability. The chromosome missegregation phenotype is fully rescued in heterozygous females but embryo viability is only partially rescued. (Endow and Komma, 1997) Homozygous oocytes show abnormal chromosome behaviour in meiosis. Bivalents segregating in different directions are seen in anaphase I. (Hatsumi and Endow, 1992)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference ncd² has lethal \| embryonic stage \| partially \| recessive phenotype, enhanceable by γTub37C¹² (Endow and Komma, 1998) ncd² has lethal \| embryonic stage \| partially \| recessive phenotype, enhanceable by γTub37C¹³ (Endow and Komma, 1998)
Other
Statement Reference αTub67C³, ncd² has female sterile phenotype (Komma and Endow, 1997, Komma and Endow, 1997, Komma and Endow, 1997)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference The ncd² inviability is dominantly enhanced by γTub37C¹³ or γTub37C¹²; ncd² females heterozygous for γTub37C¹³ or γTub37C¹² lay eggs but more than 99% fail to hatch. (Endow and Komma, 1998) ncd²/ncd² αTub67C³ females are sterile. (Komma and Endow, 1997)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	ncd⁵ (Yamamoto et al., 1989) ncd⁶ (Yamamoto et al., 1989) ncd⁷ (Yamamoto et al., 1989) ncd⁸ (Yamamoto et al., 1989) ncd^P39 (Sekelsky et al., 1999)
Partially rescued by	ncd² is partially rescued by ncd^{2.T:Avic\GFP-S65T} (Endow and Komma, 1997)
Comments	ncd²/ncd² females carrying two copies of ncd^{2.T:Avic\GFP-S65T} have poor fertility, high egg inviability and elevated chromosome missegregation. ncd²/+ females carrying one copy of ncd^{2.T:Avic\GFP-S65T} are completely rescued for chromosome segregation but partially rescued for embryo viability. (Endow and Komma, 1997) recovered as ca⁺
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Mutant does not affect ca function. (Yamamoto et al., 1989)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	ncd (Sekelsky et al., 1999, Hatsumi and Endow, 1992, Hatsumi and Endow, 1992, Yamamoto et al., 1989) ncd²
Name Synonym
Secondary FlyBase IDs

References ( 9 )
Research paper	Sekelsky et al., 1999, Genetics 152(2): 529--542 Identification of novel Drosophila meiotic genes recovered in a P-element screen. [FBrf0109059] Endow and Komma, 1998, J. Cell Sci. 111(17): 2487--2495 Assembly and dynamics of an anastral:astral spindle: the meiosis II spindle of Drosophila oocytes. [FBrf0104747] Endow and Komma, 1997, J. Cell Biol. 137(6): 1321--1336 Spindle dynamics during meiosis in Drosophila oocytes. [FBrf0094627] Komma and Endow, 1997, J. Cell Sci. 110(2): 229--237 Enhancement of the ncd^D microtubule motor mutant by mutants of Tub67C. [FBrf0092442] Endow and Komma, 1996, J. Cell Sci. 109(10): 2429--2442 Centrosome and spindle function of the Drosophila Ncd microtubule motor visualized in live embryos using Ncd-GFP fusion proteins. [FBrf0090543] Hatsumi and Endow, 1992, J. Cell Sci. 101(3): 547--559 Mutants of the microtubule motor protein, nonclaret disjunctional, affect spindle structure and chromosome movement in meiosis and mitosis. [FBrf0056468] Hatsumi and Endow, 1992, J. Cell Sci. 103(4): 1013--1020 The Drosophila ncd microtubule motor protein is spindle-associated in meiotic and mitotic cells. [FBrf0056456] Yamamoto et al., 1989, EMBO J. 8(12): 3543--3552 The claret locus in Drosophila encodes products required for eye color and for meiotic chromosome segregation. [FBrf0049629] O'Tousa and Szauter, 1980, D. I. S. 55: 119 The initial characterization of non-claret disjunctional (ncd): evidence that ca^nd is the double mutant, ca ncd. [FBrf0034326]

Allele Dmel\ncd2

Allele Dmel\ncd²