Allele Dmel\ninaA³

General Information
Symbol	Dmel\ninaA³	Species	D. melanogaster
Name		FlyBase ID	FBal0012961
Feature type	allele	Associated gene	Dmel\ninaA
Also Known As	ninaA^P269

Map ( GBrowse )
Allele class	amorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	amorphic allele - genetic evidence (Colley et al., 1991, Baker et al., 1994)
Mutagen	ethyl methanesulfonate (Schneuwly et al., 1989)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2L:878,139..878,139 evidence=experimental na_change=A878139T pr_change=H227L\|ninaA-PA reported_na_change=A1184T reported_pr_change=H227L (Schneuwly et al., 1989)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: H227L. (Schneuwly et al., 1989) Nucleotide substitution: A1184T. (Schneuwly et al., 1989)
Cytology
Phenotypic Data
Phenotypic Class
	neurophysiology defective (Rosenbaum et al., 2006)
Phenotype Manifest In
	photoreceptor cell R1 (Colley et al., 1991) photoreceptor cell R2 (Colley et al., 1991) photoreceptor cell R3 (Colley et al., 1991) photoreceptor cell R4 (Colley et al., 1991) photoreceptor cell R5 (Colley et al., 1991) photoreceptor cell R6 (Colley et al., 1991) retina \| conditional (Rosenbaum et al., 2006)
Detailed Description
	Statement Reference Mutants show light-independent retinal degeneration. Macroscopic responses of ninaA[3] photoreceptors (from dissociated ommatidia) to brief test flashes and modest light steps are greatly reduced in sensitivity compared to wild type. (Rosenbaum et al., 2006) ninaA³ mutant flies have very little Rh1 opsin in their rhabdomeres in the photoreceptor cells R1-R6, with most of the Rh1 opsin being in the ER. These cells also show large accumulations of rough ER. ninaA³, ninaE¹⁷ double mutants do not show accumulations of ER, indicating that it is not the lack of ninaA but the presence of Rh1 opsin in ninaA mutants that cause ER proliferation (ninaE is the structural gene for Rh1). Double mutants, for ninaA³ and ninaE¹⁷, which also contain Rh2^ninaE.P still display dramatic accumulations of ER membranes in photoreceptors R1-R6. (Colley et al., 1991) Dramatic reductions in rhodopsin levels of R1--R6 cells with concomitant impaired visual function. (Stamnes et al., 1991) With respect to defective PDA, ninaA² > ninaA¹ > ninaA³.
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments	The phenotype can be fully rescued by P element mediated introduction of a wild type copy of ninaA. (Stamnes et al., 1991)
Stocks ( 0 )

Notes on Origin
Discoverer	Pak.

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	269 (Colley et al., 1991) ninaA³ ninaA^P268 (Schneuwly et al., 1989) ninaA^P269 (Webel et al., 2000, Baker et al., 1994, Stamnes et al., 1991, Rosenbaum et al., 2006, Rosenbaum et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 7 )
Research paper	Rosenbaum et al., 2011, Neuron 72(4): 602--615 XPORT-Dependent Transport of TRP and Rhodopsin. [FBrf0216705] Rosenbaum et al., 2006, Neuron 49(2): 229--241 Calnexin is essential for rhodopsin maturation, Ca2+ regulation, and photoreceptor cell survival. [FBrf0191326] Webel et al., 2000, J. Biol. Chem. 275(32): 24752--24759 Role of asparagine-linked oligosaccharides in rhodopsin maturation and association with its molecular chaperone, NinaA. [FBrf0130162] Baker et al., 1994, EMBO J. 13(20): 4886--4895 The cyclophilin homolog ninaA functions as a chaperone, forming a stable complex in vivo with its protein target rhodopsin. [FBrf0076436] Colley et al., 1991, Cell 67: 255--263 The cyclophilin homolog ninaA is required in the secretory pathway. [FBrf0053360] Stamnes et al., 1991, Cell 65: 219--227 The cyclophilin homolog ninaA is a tissue-specific integral membrane protein required for the proper synthesis of a subset of Drosophila rhodopsins. [FBrf0053356] Schneuwly et al., 1989, Proc. Natl. Acad. Sci. U.S.A. 86: 5390--5394 Drosophila ninaA gene encodes an eye-specific cyclophilin (cyclosporine A binding protein). [FBrf0050847]

Allele Dmel\ninaA3

Allele Dmel\ninaA³