Allele Dmel\ninaB¹

General Information
Symbol	Dmel\ninaB¹	Species	D. melanogaster
Name		FlyBase ID	FBal0012988
Feature type	allele	Associated gene	Dmel\ninaB
Also Known As	ninaB^P315

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3R:9,527,640..9,527,640 evidence=experimental na_change=G9527640A comment=One of 3 amino acid substitutions in this strain pr_change=E280K\|ninaB-PA reported_na_change=G838A reported_pr_change=E280K (von Lintig et al., 2001) point mutation 3R:9,528,213..9,528,213 reported_na_change=A1411C comment=One of 3 amino acid substitutions in this strain evidence=experimental na_change=A9528213C pr_change=M471L\|ninaB-PA reported_pr_change=M471L (von Lintig et al., 2001) point mutation 3R:9,528,232..9,528,232 reported_pr_change=E477A comment=One of 3 amino acid substitutions in this strain evidence=experimental na_change=A9528232C pr_change=E477A\|ninaB-PA reported_na_change=A1430C (von Lintig et al., 2001)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: E280K. Nucleotide substitution: G838A. Amino acid replacement: M471L. Nucleotide substitution: A1411C. Amino acid replacement: E477A. Nucleotide substitution: A1430C. (von Lintig et al., 2001)
Cytology
Phenotypic Data
Phenotypic Class
	neurophysiology defective (Gu et al., 2004)
Phenotype Manifest In
	photoreceptor cell R1 photoreceptor cell R2 photoreceptor cell R3 photoreceptor cell R4 photoreceptor cell R5 photoreceptor cell R6 photoreceptor cell R7 photoreceptor cell R8
Detailed Description
	Statement Reference Homozygous flies lack the prolonged depolarising afterpotential (PDA) in the electroretinogram response. In mosaic flies in which the retinal tissue is homozygous for ninaB¹ but the rest of the animal is ninaB⁺, the electroretinogram response is normal (the PDA is restored). (Gu et al., 2004) The deep pseudopupil is not visible in homozygous mutant adults, only a weak shadow is detected in the eye. This phenotype can be rescued by supplementing the flies' diet with all-trans retinal. (von Lintig et al., 2001) Superficially like ninaA in aberrant visual physiology, but all eight photoreceptors in each eye facet affected by ninaB; can be restored to wild-type phenotype by dietary supplement of retinal but not by other retinoids.
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	ninaB¹ is rescued by ninaB^Scer\UAS.cGa/Scer\GAL4^elav.PLu (Wang et al., 2007) ninaB¹ is rescued by Scer\GAL4^ninaB.PW/ninaB^Scer\UAS.cGa (Wang et al., 2007) ninaB¹ is rescued by Scer\GAL4^repo/ninaB^Scer\UAS.cGa (Wang et al., 2007) ninaB¹ is rescued by Scer\GAL4^{santa-maria.PW}/ninaB^Scer\UAS.cGa (Wang et al., 2007)
Not rescued by	ninaB¹ is not rescued by Scer\GAL4^ninaD.PW/ninaB^Scer\UAS.cGa (Wang et al., 2007)
Comments
Stocks ( 1 )
Bloomington	24776 w^*/Dp(1;Y)y⁺; ninaB¹, P{UAS-ninaB.G}3
Notes on Origin
Discoverer

Comments
	Experiments in recombinant E.coli suggest that it is the G838A substitution that causes the defect. (von Lintig et al., 2001)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	ninaB¹ ninaB^P315 (Gu et al., 2004, von Lintig et al., 2001, Wang et al., 2007, Meyer et al., 2006, Yang and O'Tousa, 2007)
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Wang et al., 2007, J. Cell Biol. 177(2): 305--316 Dissection of the pathway required for generation of vitamin A and for Drosophila phototransduction. [FBrf0199300] Yang and O'Tousa, 2007, Mol. Cell. Neurosci. 35(1): 49--56 Cellular sites of Drosophila NinaB and NinaD activity in vitamin a metabolism. [FBrf0200667] Meyer et al., 2006, J. Cell Sci. 119(Pt 12): 2592--2603 Subcellular translocation of the eGFP-tagged TRPL channel in Drosophila photoreceptors requires activation of the phototransduction cascade. [FBrf0193744] Gu et al., 2004, J. Biol. Chem. 279(18): 18608--18613 Drosophila ninaB and ninaD act outside of retina to produce rhodopsin chromophore. [FBrf0174785] von Lintig et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98(3): 1130--1135 Analysis of the blind Drosophila mutant ninaB identifies the gene encoding the key enzyme for vitamin A formation in vivo. [FBrf0132439]

Allele Dmel\ninaB1

Allele Dmel\ninaB¹