Allele Dmel\norpA³⁵

General Information
Symbol	Dmel\norpA³⁵	Species	D. melanogaster
Name		FlyBase ID	FBal0013128
Feature type	allele	Associated gene	Dmel\norpA
Also Known As	norpA^P16

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Pearn et al., 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation X:4,251,818..4,251,818 evidence=experimental reported_na_change=C1733T reported_pr_change=R361C pr_change=R361C\|norpA-PD,R361C\|norpA-PA,R361C\|norpA-PB,R 361C\|norpA-PC na_change=C4251818T (Pearn et al., 1996)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Nucleotide substitution: C1733T (nucleotide numbering as in FBrf0047660). Amino acid replacement: R361C. (Pearn et al., 1996)
Cytology
Phenotypic Data
Phenotypic Class
	neurophysiology defective (Hardie et al., 2002, Wang et al., 2008) visual behavior defective \| recessive (Scott and Zuker, 1998)
Phenotype Manifest In
	photoreceptor (Scott and Zuker, 1998) photoreceptor cell (Hardie et al., 2002)
Detailed Description
	Statement Reference norpA[35] homozygous adults show extremely slow deactivation kinetics. (Wang et al., 2008) The amplitude of quantum bumps in mutant photoreceptors is smaller than wild type (when ATP is present in the intracellular solution during recordings). When ATP is absent from the intracellular solution during recordings, the amplitude of quantum bumps from mutant photoreceptors increases to values approaching wild type. (Hardie et al., 2002) The size and shape of the quantum bumps are unaffected, although the latency is significantly different from wild type. (Scott and Zuker, 1998) The electroretinogram (ERG) of norpA³⁵ flies shows reduced amplitude and slower response kinetics compared to that of wild-type flies. (Pearn et al., 1996) The amplitude response to vapours of all odorants tested in the maxillary palp is not significantly different from wild type. The antennal response is also unaffected. (Riesgo-Escovar et al., 1995) intermediate ERG
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference norpA³⁵ has neurophysiology defective phenotype, suppressible by rdgA¹ (Hardie et al., 2002)
NOT suppressed by
Statement Reference norpA³⁵ has neurophysiology defective phenotype, non-suppressible by mts^XE-2258/mts[+] (Wang et al., 2008)
Phenotype Manifest In
Suppressed by
Statement Reference norpA³⁵ has photoreceptor cell phenotype, suppressible by rdgA¹ (Hardie et al., 2002)
Suppressor of
Statement Reference norpA³⁵ is a suppressor of rhabdomere phenotype of rdgA¹ (Georgiev et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference The homozygous norpA[35] deactivation defects are not rescued when in combination with mts[XE-2258]/+. (Wang et al., 2008) norpA[35] almost completely suppresses the retinal degeneration seen in rdgA[1] flies. (Georgiev et al., 2005) The small amplitude of quantum bumps in norpA³⁵ photoreceptors (seen when ATP is present in the intracellular solution during recordings) is increased in size to near wild-type levels if the flies are also mutant for rdgA¹. (Hardie et al., 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	9049 w^* norpA³⁵
Notes on Origin
Discoverer	Pak.

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	norpA³⁵ norpA^P16 (Hardie et al., 2003, Hardie et al., 2002, Scott and Zuker, 1998, Pearn et al., 1996, Riesgo-Escovar et al., 1995, Pearn et al., 1991, Georgiev et al., 2005, Wang et al., 2008) x16
Name Synonym
Secondary FlyBase IDs

References ( 10 )
Research paper	Wang et al., 2008, J. Neurosci. 28(6): 1444--1451 Role of protein phosphatase 2A in regulating the visual signaling in Drosophila. [FBrf0202809] Georgiev et al., 2005, J. Cell Sci. 118(Pt 7): 1373--1384 Functional INAD complexes are required to mediate degeneration in photoreceptors of the Drosophila rdgA mutant. [FBrf0184068] Hardie et al., 2003, J. Biol. Chem. 278(21): 18851--18858 Rescue of light responses in the Drosophila 'null' phospholipase C mutant, norpA[P24], by the diacylglycerol kinase mutant, rdgA, and by metabolic inhibition. [FBrf0159791] Hardie et al., 2002, Neuron 36(4): 689--701 Molecular basis of amplification in Drosophila phototransduction. Roles for g protein, phospholipase C, and diacylglycerol kinase. [FBrf0152274] Scott and Zuker, 1998, Nature 395(6704): 805--808 Assembly of the Drosophila phototransduction cascade into a signalling complex shapes elementary responses. [FBrf0105321] Pearn et al., 1996, J. Biol. Chem. 271(9): 4937--4945 Molecular, biochemical, and electrophysiological characterization of Drosophila norpA mutants. [FBrf0086575] Riesgo-Escovar et al., 1995, Proc. Natl. Acad. Sci. U.S.A. 92(7): 2864--2868 Requirement for a phospholipase C in odor response: Overlap between olfaction and vision in Drosophila. [FBrf0080352] Bloomquist et al., 1988, Cell 54: 723--733 Isolation of a putative phospholipase C gene of Drosophila, norpA, and its role in phototransduction. [FBrf0047660] Pak et al., 1970, Nature 227: 518--520 Mutants of the visual pathway of Drosophila melanogaster. [FBrf0021874]
Abstract	Pearn et al., 1991, Greenspan, Palka, 1991: 142 Molecular characterization of norpA alleles. [FBrf0079165]

Allele Dmel\norpA35

Allele Dmel\norpA³⁵