|Feature type||allele||Associated gene||Dmel\br|
|Also Known As||npr13, l(1)npr-1, npr-1, npr3|
|Allele class||loss of function allele, amorphic allele - genetic evidence|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Phenotype Manifest In|
wing margin & sensory neuron | somatic clone
100% of brrbp-2/brnpr-3 larvae are able to pupate, but ~15% die as early pupae. 80% of these survive to late pharate adult stage, with ~20% failing to eclose. brnpr-3/+ flies show the same susceptibility to methoprene as wild-type flies.
brnpr-3/+ flies show a wild-type number of DLM fibers and normal TDT patterning in the adult thorax.
The wing marginal sensory neurons fail to differentiate in brnpr-3 mutant clones and brnpr-3 Minute mutant clones. The loss of the marginal neurons is permanent as chemosensory bristles are never observed in adult wings with brnpr-3 clones encompassing the wing margin.
brnpr-3 mutants exhibit a significant reduction in encapsulation response. Lymph glands of older, developmentally arrested mutant larvae are of normal size and do not show hypertrophy.
brnpr-3/Y eye discs show ommatidial disorganisation and signs of furrow failure, including mature ommatidial clusters at the furrow. Homozygous clones in the eye disc show defects in ommatidial organisation, including the wrong number of photoreceptors in clusters. Excess numbers of R8 cells in a cluster are seen. Homozygous clones spanning the morphogenetic furrow are not associated with any retardation of the furrow. Clones at the posterior margin of the disc do not show any visible defects in furrow initiation. Homozygous clones in the adult eye result in a scar.
Homozygotes arrest development at the end of the larval period, do not pupariate and die after several days as wandering third instar larvae.
Lethality acts in the late third instar.
Lethality acts at the late third instar stage.
Failure of salivary gland degeneration. Reduction in dorso-ventral class of indirect flight muscles.
Wandering third instar larvae initially show normal CNS histology, later widespread loss of tissue integrity is seen.
Imaginal discs of homozygous larvae are normal.
Imaginal discs form swollen vesicles.
No maternal effect.
Larvae do not pupate, but can survive for weeks after reaching the size of the mature wild-type larva. Larval imaginal discs are normal on the 4th day after hatching, however they become bloated and a highly distorted by the 8th-10th day of larval life. The ring gland is normal in size on the 8th-10th day of larval life.
|Phenotype Manifest In|
brnpr-3 has mesothoracic extracoxal depressor muscle 66 phenotype, enhanceable by Mef2[+]/twi1/Mef2P544/twi[+]
brnpr-3/br[+] is an enhancer of mesothoracic extracoxal depressor muscle 66 phenotype of Mef2P544, twi1/twi[+]
The lethality is enhanced in brrbp-2/brnpr-3 Rst(1)JH3/Rst(1)JH3 double mutants compared to brrbp-2/brnpr-3 mutants, with no flies surviving beyond the pupal stage.
There is a significant reduction in the number of DLM fibers in brnpr-3/+; twi1/+ double mutants (5.8 fibers vs 6 in wild type) and an even greater reduction in brnpr-3/+; twi1/+, Mef2P544/+ triple mutants (5.2 fibers). However, no such reduction is seen in brnpr-3/+; Mef2P544/+ double mutants. brnpr-3/+; Mef2P544/+ double heterozygotes display higher levels of defects in TDT organization than either single heterozygote, including the presence of ectopic fibers in the lumen. 82% of brnpr-3/+; twi1/+, Mef2P544/+ triple mutants show defects in TDT patterning (a higher level than any of the double mutant combinations), including additional small cells, internal fibers and the rerouting of some fibers to the periphery.
|Complementation & Rescue Data|
|Partially rescued by|
|Not rescued by|
Lethality is not rescued by brBRcore.Q1.Z1.hs, brBRcore.TNT.Q1.Z1.hs, brZ2.hs, brBRcore.NS.Z3.hs or brBRcore.Z4.hs when expressed using heat shocks of either 33oC or 37oC. brnpr-3 third instar larvae expressing brZ2.hs show further development than control brnpr-3 larvae, forming normal looking puparia with everted spiracles, a tanned cuticle and imaginal discs that have initiated morphogenesis.
|Stocks ( 3 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 11 )|
|Secondary FlyBase IDs|
|References ( 41 )|
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|Recent research papers (0)|
|All research papers listed in FlyBase were published before 2011|
|Recent reviews (0)|
|All reviews listed in FlyBase were published before 2011|