FlyBase Allele Report: Dmel\br[npr-3]

General Information

Symbol

Dmel\br^npr-3

Species

D. melanogaster

Name

FlyBase ID

FBal0013157

Feature type

allele

Associated gene

Dmel\br

Associated Insertion(s)

Carried in Construct

Also Known As

npr1³, l(1)npr-1, br^npr3, npr³, npr-1

Key Links

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Restifo and Merrill, 1994, Gotwals and Fristrom, 1991, Restifo and White, 1991)

loss of function allele

(Wilson et al., 2006, Schubiger et al., 2005, Bayer et al., 1997, Crossgrove et al., 1996)

Mutagen

ethyl methanesulfonate

(Bayer et al., 1997, Restifo and Merrill, 1994, Gotwals and Fristrom, 1991, Belyaeva et al., 1980)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

100% of br^rbp-2/br^npr-3 larvae are able to pupate, but ~15% die as early pupae. 80% of these survive to late pharate adult stage, with ~20% failing to eclose. br^npr-3/+ flies show the same susceptibility to methoprene as wild-type flies.

(Wilson et al., 2006)

br^npr-3/+ flies show a wild-type number of DLM fibers and normal TDT patterning in the adult thorax.

(Lovato et al., 2005)

The wing marginal sensory neurons fail to differentiate in br^npr-3 mutant clones and br^npr-3 Minute mutant clones. The loss of the marginal neurons is permanent as chemosensory bristles are never observed in adult wings with br^npr-3 clones encompassing the wing margin.

(Schubiger et al., 2005)

br^npr-3 mutants exhibit a significant reduction in encapsulation response. Lymph glands of older, developmentally arrested mutant larvae are of normal size and do not show hypertrophy.

(Sorrentino et al., 2002)

br^npr-3/Y eye discs show ommatidial disorganisation and signs of furrow failure, including mature ommatidial clusters at the furrow. Homozygous clones in the eye disc show defects in ommatidial organisation, including the wrong number of photoreceptors in clusters. Excess numbers of R8 cells in a cluster are seen. Homozygous clones spanning the morphogenetic furrow are not associated with any retardation of the furrow. Clones at the posterior margin of the disc do not show any visible defects in furrow initiation. Homozygous clones in the adult eye result in a scar.

(Brennan et al., 2001)

Homozygotes arrest development at the end of the larval period, do not pupariate and die after several days as wandering third instar larvae.

(Bayer et al., 1997)

Lethality acts in the late third instar.

(Emery et al., 1994)

The Pig1 to Sgs4 switch fails to occur during mid-third instar.

(von Kalm et al., 1994)

Lethality acts at the late third instar stage.

(Karim et al., 1993)

Failure of salivary gland degeneration. Reduction in dorso-ventral class of indirect flight muscles.

(Restifo and White, 1992)

18--28% penetrance of zip interaction with br^npr-3 heterozygous females.

(Gotwals and Fristrom, 1991)

Wandering third instar larvae initially show normal CNS histology, later widespread loss of tissue integrity is seen.

(Restifo and White, 1991)

Imaginal discs of homozygous larvae are normal.

(Gatti and Baker, 1989)

Imaginal discs form swollen vesicles.

(Kiss et al., 1988)

No maternal effect.

(Perrimon et al., 1984)

Larvae do not pupate, but can survive for weeks after reaching the size of the mature wild-type larva. Larval imaginal discs are normal on the 4th day after hatching, however they become bloated and a highly distorted by the 8th-10th day of larval life. The ring gland is normal in size on the 8th-10th day of larval life.

(Kiss et al., 1978)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

br^rbp-2/br^npr-3 has lethal - all die during P-stage phenotype, enhanceable by Met³/Met³

(Wilson et al., 2006)

Enhancer of

Statement

Reference

br^npr-3/br[+] is an enhancer of visible | dominant phenotype of Bar¹

(Brennan et al., 2001)

Suppressor of

Statement

Reference

br^npr-3/br^npr-3 is a suppressor of abnormal cell number | somatic clone | P-stage phenotype of EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^αTub84B.PL

(Zhang et al., 2024)

Phenotype Manifest In

Enhanced by

Statement

Reference

Mef2^P544, br^npr-3/br[+] has mesothoracic tergotrochanter muscle cell phenotype, enhanceable by twi¹/twi[+]

(Lovato et al., 2005)

br^npr-3/br[+], twi¹ has dorsal longitudinal indirect flight muscle cell phenotype, enhanceable by Mef2[+]/Mef2^P544

(Lovato et al., 2005)

br^npr-3 has mesothoracic tergotrochanter muscle cell phenotype, enhanceable by Mef2[+]/twi¹/Mef2^P544/twi[+]

(Lovato et al., 2005)

Enhancer of

Statement

Reference

br^npr-3/br[+] is an enhancer of mesothoracic tergotrochanter muscle cell phenotype of Mef2^P544, twi¹/twi[+]

(Lovato et al., 2005)

br^npr-3/br[+] is an enhancer of mesothoracic tergotrochanter muscle cell phenotype of Mef2^P544

(Lovato et al., 2005)

Mef2[+], br^npr-3, Mef2^P544, br[+] is an enhancer of mesothoracic tergotrochanter muscle cell phenotype of twi¹

(Lovato et al., 2005)

br^npr-3/br[+] is an enhancer of eye phenotype of Bar¹

(Brennan et al., 2001)

Suppressor of

Statement

Reference

br^npr-3/br^npr-3 is a suppressor of enteroendocrine cell | somatic clone | P-stage | decreased number phenotype of EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^αTub84B.PL

(Zhang et al., 2024)

Other

Statement

Reference

Mef2^P544, br^npr-3/br[+], twi¹/twi[+] has dorsal longitudinal indirect flight muscle cell phenotype

(Lovato et al., 2005)

Mef2[+]/Mef2^P544, br^npr-3, twi¹/twi[+] has dorsal longitudinal indirect flight muscle cell phenotype

(Lovato et al., 2005)

br^npr-3/br[+], twi¹ has dorsal longitudinal indirect flight muscle cell phenotype

(Lovato et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The lethality is enhanced in br^rbp-2/br^npr-3 Rst(1)JH³/Rst(1)JH³ double mutants compared to br^rbp-2/br^npr-3 mutants, with no flies surviving beyond the pupal stage.

(Wilson et al., 2006)

There is a significant reduction in the number of DLM fibers in br^npr-3/+; twi¹/+ double mutants (5.8 fibers vs 6 in wild type) and an even greater reduction in br^npr-3/+; twi¹/+, Mef2^P544/+ triple mutants (5.2 fibers). However, no such reduction is seen in br^npr-3/+; Mef2^P544/+ double mutants.

br^npr-3/+; Mef2^P544/+ double heterozygotes display higher levels of defects in TDT organization than either single heterozygote, including the presence of ectopic fibers in the lumen. 82% of br^npr-3/+; twi¹/+, Mef2^P544/+ triple mutants show defects in TDT patterning (a higher level than any of the double mutant combinations), including additional small cells, internal fibers and the rerouting of some fibers to the periphery.

(Lovato et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

br^npr-3 is partially rescued by br^Z2.hs

(Bayer et al., 1997)

Not rescued by

br^npr-3 is not rescued by br^BRcore.Z4.hs

(Bayer et al., 1997)

br^npr-3 is not rescued by br^{BRcore.Q1.Z1.hs}

(Bayer et al., 1997)

br^npr-3 is not rescued by br^{BRcore.NS.Z3.hs}

(Bayer et al., 1997)

br^npr-3 is not rescued by br^{BRcore.TNT.Q1.Z1.hs}

(Bayer et al., 1997)

Comments

Lethality is not rescued by br^{BRcore.Q1.Z1.hs}, br^{BRcore.TNT.Q1.Z1.hs}, br^Z2.hs, br^{BRcore.NS.Z3.hs} or br^BRcore.Z4.hs when expressed using heat shocks of either 33^oC or 37^oC. br^npr-3 third instar larvae expressing br^Z2.hs show further development than control br^npr-3 larvae, forming normal looking puparia with everted spiracles, a tanned cuticle and imaginal discs that have initiated morphogenesis.

(Bayer et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

5964

y¹ br^npr-3 w¹/Binsn

29971

y¹ br^npr-3 w^a sn³/Binsn

Kyoto

108509

y¹ w¹ br^npr-3/Binsn

Notes on Origin

Discoverer

Kiss.

Comments

Analysis of Ecol\lacZ^Sgs3.GLX3.3 expression in salivary glands mosaic for br^npr-3 suggests a cell-autonomous requirement for br⁺ function for the expression of Sgs3 in the salivary gland.

(Vijay Raghavan et al., 1988)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

Br^npr3

(Syed et al., 2017)

br^npr-3

(Zhang et al., 2024, Kitatani et al., 2020, Jia et al., 2014, Xiang et al., 2010, Zartman et al., 2008, Ward et al., 2006, Lovato et al., 2005)

br^npr3

(Guan et al., 2022, Guo et al., 2016, Zhou et al., 2009)

br^npr

(Jia et al., 2015)

broad^npr-3

(Eliazer et al., 2014)

l(1)d.norm.-12

l(1)dn12

l(1)npr-1

(Hofmann et al., 1991, Vijay Raghavan et al., 1988, Belyaeva et al., 1982, Belyaeva et al., 1980, Kiss et al., 1978)

npr-1

(Solov'eva, 1992, Mazina et al., 1990, Solovyeva and Belyaeva, 1989)

npr1

npr1³

(Spokony and Restifo, 2009, Wilson et al., 2006, Brennan et al., 2001, Lee et al., 2000, Liu and Restifo, 1998, Bayer et al., 1997, Crossgrove et al., 1996, Lehmann and Korge, 1996, Restifo et al., 1995, Restifo and Merrill, 1994, Kiss et al., 1988)

npr³

(Bernardi et al., 2009, Schubiger et al., 2005, Renault et al., 2001)

nrp l[3]

(Dunne et al., 2002)

Name Synonyms

lethal(1)discs normal

Secondary FlyBase IDs

References (50)

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