Allele Dmel\Nrg^l7

General Information
Symbol	Dmel\Nrg^l7	Species	D. melanogaster
Name		FlyBase ID	FBal0013172
Feature type	allele	Associated gene	Dmel\Nrg
Also Known As	nrg², Nrg¹⁷, l(1)VA142, VA142

Allele class	amorphic allele - genetic evidence, hypomorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	amorphic allele - genetic evidence (Hortsch et al., 1989) hypomorphic allele - genetic evidence (Bieber et al., 1989, Hall and Bieber, 1997, )
Mutagen	ethyl methanesulfonate (Bieber et al., 1989, Hortsch et al., 1989, Hall and Bieber, 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: W80@. (Yamamoto et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage (Bieber et al., 1989) lethal \| embryonic stage \| recessive (Perrimon et al., 1989, Hall and Bieber, 1997) lethal \| heat sensitive (with Nrg^l10) (Carhan et al., 2005) lethal \| larval stage (Bieber et al., 1989) neuroanatomy defective (Yamamoto et al., 2006, Yamamoto, 2006) neuroanatomy defective (with Nrg^{Scer\UAS.T:Ivir\HA1}), with Scer\GAL4^IG1-1 (Yamamoto et al., 2006)
Phenotype Manifest In
	abdominal segmental nerve (Hall and Bieber, 1997) aCC neuron (Hall and Bieber, 1997) dorsal multidendritic neuron ddaD & axon (Yamamoto et al., 2006, Yamamoto, 2006) dorsal multidendritic neuron ddaD & dendritic tree (Yamamoto et al., 2006, Yamamoto, 2006) dorsal multidendritic neuron ddaE & axon (Yamamoto et al., 2006, Yamamoto, 2006) dorsal multidendritic neuron ddaE & dendritic tree (Yamamoto et al., 2006) dorsal multidendritic neuron ddaE & dendritic tree (with Nrg^{Scer\UAS.T:Ivir\HA1}), with Scer\GAL4^IG1-1 (Yamamoto et al., 2006) dorsal trunk primordium (Paul et al., 2003) embryonic ganglionic branch & embryonic tracheole (Paul et al., 2003) peripheral nervous system (Hall and Bieber, 1997)
Detailed Description
	Statement Reference 56% of dorsal da neuron E (ddaE) and 14% of ddaD neurons show ectopic branches on their axons at 19-20 hours after egg laying (AEL) in Nrg^l7 embryos. In addition, the dendritic arbors of ddaD and ddaE are deformed and this phenotype can be detected at 18-19 hours AEL. Embryos that express Nrg^{Scer\UAS.T:Ivir\HA1}, under the control of Scer\GAL4^IG1-1, in a Nrg^l7 background and Scer\GAL4^repo show deformed ddaE dendritic arbors. (Yamamoto et al., 2006) 56% of dorsal da neuron E (ddaE) and 14% of ddaD neurons show ectopic branches on their axons in Nrg^l3 embryos. The dendritic branches of ddaD cover a smaller field and have fewer terminals than wild-type ddaD neurons. (Yamamoto, 2006) Nrg[l10]/Nrg[l7] females are viable at 18[o]C but are inviable at 25[o]C. Nrg[849]/Nrg[l7] and Nrg[892]/Nrg[l7] females show normal sexual receptivity when mated to wild-type males. (Carhan et al., 2005) In Nrg^l7 homozygous embryos, tracheal phenotypes are apparent from stage 15. At stage 16 in these embryos the average dorsal trunk length is significantly greater than wild type (P<0.005) (121+/-2% mean+/-s.e.m., n>5, normalized to stage 16 wild-type value). In addition these embryos have moderately severe diameter increases in the dorsal trunk and other primary tracheal branches, and some ganglionic branches exhibit missing lumen. Unlike wild-type, post stage 15 trachea in Nrg^l7 homozygotes are unable to exclude from their lumens, a fluorescently labelled 10kDa dextran injected into the body cavity. This is consistent with these trachea lacking a functioning septate junction barrier. (Paul et al., 2003) Homozygous embryos show a general disorganisation of the peripheral nervous system cell bodies. The aCC and SNb motoneurons often show a stalled phenotype, failing to extend normally, and SNb motoneurons sometimes show abnormal contacts with their targets. (Hall and Bieber, 1997) Nrg^l7 is lethal during the late embryonic and early larval phase of development. It has no maternal effect component. (Bieber et al., 1989)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference Nrg^l7/Nrg[+] is an enhancer of neuroanatomy defective \| larval stage phenotype of Nak^{dsRNA.Scer\UAS}, Scer\GAL4^elav-C155 (Yang et al., 2011)
Phenotype Manifest In
Enhancer of
Statement Reference Nrg^l7/Nrg[+] is an enhancer of dendrite \| larval stage phenotype of Nak^{dsRNA.Scer\UAS}, Scer\GAL4^elav-C155 (Yang et al., 2011) Nrg^l7/Nrg[+] is an enhancer of dendritic arborizing neuron \| larval stage phenotype of Nak^{dsRNA.Scer\UAS}, Scer\GAL4^elav-C155 (Yang et al., 2011) Nrg^l7 is an enhancer of axon \| embryonic stage phenotype of Nrt¹ (Speicher et al., 1998) Nrg^l7 is an enhancer of phenotype of Nrt⁵ (Speicher et al., 1998)
Additional Comments
Genetic Interactions
Statement Reference Nrg[l7] dominantly enhances the shortening of dendritic length and the reduction in the number of dendritic endpoints of dorsal dendritic arborisation neurons which is seen in larvae expressing Nak[dsRNA.Scer\UAS] under the control of Scer\GAL4[elav-C155]. (Yang et al., 2011) Increases the frequency of Fas2 expressing axons showing defects in Nrt¹/Nrt⁵, Nrt¹/Nrt¹ and Nrt⁵/Nrt⁵ embryos. (Speicher et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Complements	Nrg^ibx (Carhan et al., 2005)
Fails to complement	Nrg^l4 (Hall and Bieber, 1997) Nrg^l10 (Hall and Bieber, 1997)
Rescued by	Nrg^l7 is rescued by Nrg^{Ubi-p63E.T:Ivir\HA1} (Yamamoto et al., 2006)
Partially rescued by	Nrg^l7 is partially rescued by Scer\GAL4^repo/Scer\GAL4^IG1-1/Nrg^{Scer\UAS.T:Ivir\HA1} (Yamamoto et al., 2006)
Comments	The reduced female receptivity phenotype of Nrg[ibx] is complemented by Nrg[l7]. Nrg[l7] complements the reduced female receptivity phenotype of Nrg[892]. (Carhan et al., 2005)
Stocks ( 2 )
Bloomington	5595 Nrg^l7/FM7c
Kyoto	108320 Nrg^l7/FM7c
Notes on Origin
Discoverer	Lefevre.

Comments
	Complements: ibx^M72. (Kerr et al., 1997) Maternal germline clonal analysis demonstrates there is no maternal effect. (Perrimon et al., 1989)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	l(1)7Fa⁷ l(1)VA142 (Kerr et al., 1997, Bieber et al., 1989, ) nrg² (Speicher et al., 1998, Hall and Bieber, 1997, Yamamoto et al., 2006, Yamamoto, 2006, Carhan et al., 2005) Nrg⁷ (Kerr et al., 1997) Nrg¹⁷ (Read et al., 2005, Paul et al., 2003) nrg¹⁷ (Yang et al., 2011) Nrg^l7 (Mao and Freeman, 2009) VA142 (Hortsch et al., 1989, Perrimon et al., 1989)
Name Synonym
Secondary FlyBase IDs

References ( 13 )
Research paper	Yang et al., 2011, Neuron 72(2): 285--299 Nak regulates localization of clathrin sites in higher-order dendrites to promote local dendrite growth. [FBrf0216477] Mao and Freeman, 2009, Development 136(3): 473--481 Fasciclin 2, the Drosophila orthologue of neural cell-adhesion molecule, inhibits EGF receptor signalling. [FBrf0206540] Yamamoto et al., 2006, Curr. Biol. 16(16): 1678--1683 Control of axonal sprouting and dendrite branching by the Nrg-Ank complex at the neuron-glia interface. [FBrf0193279] Carhan et al., 2005, Genes Brain Behav. 4(8): 449--465 Female receptivity phenotype of icebox mutants caused by a mutation in the L1-type cell adhesion molecule neuroglian. [FBrf0190532] Read et al., 2005, Genetics 171(3): 1057--1081 A Drosophila model of multiple endocrine neoplasia type 2. [FBrf0190765] Paul et al., 2003, Development 130(20): 4963--4974 The Na+/K+ ATPase is required for septate junction function and epithelial tube-size control in the Drosophila tracheal system. [FBrf0162083] Speicher et al., 1998, Neuron 20(2): 221--233 Neurotactin functions in concert with other identified CAMs in growth cone guidance in Drosophila. [FBrf0100805] Hall and Bieber, 1997, J. Neurobiol. 32(3): 325--340 Mutations in the Drosophila Neuroglian cell adhesion molecule affect motor neuron pathfinding and peripheral nervous system patterning. [FBrf0092545] Kerr et al., 1997, J. Neurogenet. 11(3-4): 213--229 Icebox, a recessive X-linked mutation in Drosophila causing low sexual receptivity. [FBrf0128523] Bieber et al., 1989, Cell 59(3): 447--460 Drosophila neuroglian: a member of the immunoglobulin superfamily with extensive homology to the vertebrate neural adhesion molecule L1. [FBrf0049360] Perrimon et al., 1989, Genetics 121: 333--352 Zygotic lethals with specific maternal effect phenotypes in Drosophila melanogaster. [FBrf0049894]
Supplementary material	Yamamoto, 2006, Curr. Biol. 16(16): [FBrf0199093]
Abstract	Hortsch et al., 1989, Europ. Dros. Res. Conf. 11: 247 Drosophila neuroglian: a member of the immunoglobulin superfamily with extensive homology to the vertebrate neural adhesion molecule L1. [FBrf0064307]

Allele Dmel\Nrgl7

Allele Dmel\Nrg^l7