Allele Dmel\Nrg^l10

General Information
Symbol	Dmel\Nrg^l10	Species	D. melanogaster
Name		FlyBase ID	FBal0013175
Feature type	allele	Created / Updated	2006-08-22/2006-08-22
Associated gene	Dmel\Nrg

Allele class
Mutagen	ethyl methanesulfonate
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (King et al., 1986, Hall and Bieber, 1997)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Assay mode
Cytology
Phenotypic Data
Phenotypic Class
	lethal (King et al., 1986) lethal \| recessive \| conditional - heat sensitive (Garcia-Alonso et al., 2000) lethal \| embryonic stage \| recessive \| conditional - heat sensitive (Hall and Bieber, 1997, Bouley et al., 2000)
Phenotype Manifest In
	axon & ocellus sensory structure \| conditional ts (Garcia-Alonso et al., 2000) axon & mechanosensory neuron & adult head \| conditional ts (Garcia-Alonso et al., 2000) abdominal 1 lateral pentascolopidial chordotonal organ lch5 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 2 lateral pentascolopidial chordotonal organ lch5 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 3 lateral pentascolopidial chordotonal organ lch5 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 4 lateral pentascolopidial chordotonal organ lch5 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 5 lateral pentascolopidial chordotonal organ lch5 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 6 lateral pentascolopidial chordotonal organ lch5 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 7 lateral pentascolopidial chordotonal organ lch5 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 1 lateral sensillum campaniformium lc1 & larva \| conditional ts (Hall and Bieber, 1997) abdominal 2 lateral sensillum campaniformium lc1 & larva \| conditional ts (Hall and Bieber, 1997) abdominal 3 lateral sensillum campaniformium lc1 & larva \| conditional ts (Hall and Bieber, 1997) abdominal 4 lateral sensillum campaniformium lc1 & larva \| conditional ts (Hall and Bieber, 1997) abdominal 5 lateral sensillum campaniformium lc1 & larva \| conditional ts (Hall and Bieber, 1997) abdominal 6 lateral sensillum campaniformium lc1 & larva \| conditional ts (Hall and Bieber, 1997) abdominal 7 lateral sensillum campaniformium lc1 & larva \| conditional ts (Hall and Bieber, 1997) abdominal 1 lateral sensillum trichodeum lh1 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 1 lateral sensillum trichodeum lh2 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 2 lateral sensillum trichodeum lh1 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 2 lateral sensillum trichodeum lh2 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 3 lateral sensillum trichodeum lh1 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 3 lateral sensillum trichodeum lh2 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 4 lateral sensillum trichodeum lh1 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 4 lateral sensillum trichodeum lh2 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 5 lateral sensillum trichodeum lh1 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 5 lateral sensillum trichodeum lh2 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 6 lateral sensillum trichodeum lh1 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 6 lateral sensillum trichodeum lh2 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 7 lateral sensillum trichodeum lh1 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 7 lateral sensillum trichodeum lh2 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 1 lateral monoscolopidial chordotonal organ lch1 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 2 lateral monoscolopidial chordotonal organ lch1 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 3 lateral monoscolopidial chordotonal organ lch1 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 4 lateral monoscolopidial chordotonal organ lch1 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 5 lateral monoscolopidial chordotonal organ lch1 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 6 lateral monoscolopidial chordotonal organ lch1 \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal 7 lateral monoscolopidial chordotonal organ lch1 \| conditional - heat sensitive (Hall and Bieber, 1997) aCC neuron \| conditional - heat sensitive (Hall and Bieber, 1997) abdominal posterior fascicle \| conditional - heat sensitive (Hall and Bieber, 1997) motor neuron \| conditional - heat sensitive (Bouley et al., 2000)
Detailed Description
	Statement Reference Homozygotes are viable at 18^oC and 21^oC. At 25^oC, viability of homozygous embryos is 22% and those embryos that do hatch die as first instar larvae shortly after hatching. No homozygous embryos survive to hatching at 29^oC. The temperature-sensitive period for lethality is between midstage 15 and late stage 16 of embryonic development. Homozygous embryos show a general disorganisation of the peripheral nervous system cell bodies when raised at the restrictive temperature of 29^oC. The lateral pentascolopidial chordotonal organ (lch5) cells are rounded and irregularly positioned. The apical dendrites and basal anchoring process are disorganised. The disorganisation of the lch5 appears to displace most of the other cells of the lateral cluster, including the lateral campaniform sensilla, the lateral trichoid sensilla and the lateral monoscolopidial chordotonal organ. Fasciculation of the peripheral sensory axon tracts appears relatively normal. The aCC and SNb motoneurons often show a stalled phenotype, failing to extend normally, and SNb motoneurons sometimes show abnormal contacts with their targets. (Hall and Bieber, 1997) Homozygous embryos raised at the restrictive temperature show altered motoneuron pathfinding trajectories. (Bouley et al., 2000) When Nrg^l10 embryos and larvae are raised at the permissive temperature (17^oC) and shifted to the restrictive temperature (29^oC) during early to middle third instar larvae, abnormalities are seen in ocellar sensory system (OSS) axon pathfinding without alterations in cell number or position. Ocellar pioneer (OP) axons become attached to the epidermis, where they can fasciculate with bristle mechanosensory (BM) axons. Secondly OP axons fail to cross the brain at the appropriate choice point. After head eversion, this alteration is revealed as an OP nerve projecting inside the head. free and away from the epidermis but heading and terminating at an ectopic position. Thirdly BM axons take abnormal trajectories. Although they initially project in the correct direction, sometimes when reaching the region where they should leave the epidermal surface and project to the brain, they turn backward and project away from their normal trajectory. Forth BM axons sometimes stall in the epidermis. Fifth, some BM axons, affter head eversion, project separated from the epidermis. (Garcia-Alonso et al., 2000)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference Nrg^l10 has axon & mechanosensory neuron & adult head \| conditional ts phenotype, enhanceable by Scer\GAL4^MS1075/htl^{Scer\UAS.T:λ\cI-DD} (Garcia-Alonso et al., 2000) Nrg^l10 has axon & ocellus sensory structure \| conditional ts phenotype, enhanceable by Egfr^unspecified (Garcia-Alonso et al., 2000) Nrg^l10 has axon & mechanosensory neuron & adult head \| conditional ts phenotype, enhanceable by Egfr^unspecified (Garcia-Alonso et al., 2000)
Suppressed by
Statement Reference Nrg^l10 has axon & adult head \| conditional ts phenotype, suppressible \| partially by Scer\GAL4^MS1075/htl^{Scer\UAS.T:λ\cI-DD} (Garcia-Alonso et al., 2000) Nrg^l10 has axon & adult head \| conditional ts phenotype, suppressible \| partially by htl^Scer\UAS.cMa/Scer\GAL4^MS1075 (Garcia-Alonso et al., 2000) Nrg^l10 has axon & ocellus sensory structure \| conditional ts phenotype, suppressible by Egfr^E3 (Garcia-Alonso et al., 2000) Nrg^l10 has axon & mechanosensory neuron & adult head \| conditional ts phenotype, suppressible by Egfr^E3 (Garcia-Alonso et al., 2000)
Additional Comments
Genetic Interactions
Statement Reference The addition of htl^{Scer\UAS.T:λ\cI-DD} (when driven by Scer\GAL4^MS1075) to Nrg^l10 animals reduces the expressivity and penetrance of axon guidance defects. The frequency of axon guidance alterations are halved, though bristle mechanosensory (BM) axon extension defects are significantly enhanced. The addition of htl^Scer\UAS.cMa (when driven by Scer\GAL4^MS1075) to Nrg^l10 animals reduces the expressivity of axon guidance defects. The addition of htl^AB42 to Nrg^l10 animals shows a very mild enhancement of the axon guidance defects. The addition of Egfr^unspecified to Nrg^l10 animals shows an enhancement of the axon guidance defects. The addition of Egfr^E3 or Egfr^E1 to Nrg^l10 animals shows a suppression of the axon guidance defects. (Garcia-Alonso et al., 2000)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Nrg^l4 (Hall and Bieber, 1997) Nrg^l7 (Hall and Bieber, 1997)
Partially rescued by	Nrg^l10 is partially rescued by Scer\GAL4^MS1075/Nrg^180.Scer\UAS (Garcia-Alonso et al., 2000)
Comments
Stocks ( 1 )
Bloomington	24167 C(1)A, y¹/y¹ w^* Nrg^l10
Notes on Origin
Discoverer	Digan. Digan.

Comments
	Complements: ibx^M72. (Kerr et al., 1997)
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	l(1)7Fa¹⁰ l(1)B4 (Kerr et al., 1997, King et al., 1986, ) nrg³ (Kang et al., 2001, Garcia-Alonso et al., 2000, Bouley et al., 2000, Hall and Bieber, 1997) Nrg¹⁰ (Kerr et al., 1997) Nrg^l10
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Bouley et al., 2000, J. Neurosci. 20(12): 4515--4523 The L1-type cell adhesion molecule neuroglian influences the stability of neural ankyrin in the Drosophila embryo but not its axonal localization. [FBrf0128409] Garcia-Alonso et al., 2000, Neuron 28(3): 741--752 The EGF and FGF receptors mediate neuroglian function to control growth cone decisions during sensory axon guidance in Drosophila. [FBrf0132430] Hall and Bieber, 1997, J. Neurobiol. 32(3): 325--340 Mutations in the Drosophila Neuroglian cell adhesion molecule affect motor neuron pathfinding and peripheral nervous system patterning. [FBrf0092545] Kerr et al., 1997, J. Neurogenet. 11(3-4): 213--229 Icebox, a recessive X-linked mutation in Drosophila causing low sexual receptivity. [FBrf0128523] King et al., 1986, Dev. Genet. 7: 1--20 Complementation between alleles at the ovarian tumor locus of Drosophila melanogaster. [FBrf0044177]
Abstract	Kang et al., 2001, Bellen, Taylor, 2001: 189 CRASH in flies: The L1 CAM homolog neuroglian is essential for mushroom body and central complex development. [FBrf0144562]

Allele Dmel\Nrgl10

Allele Dmel\Nrg^l10