Allele Dmel\nub²

General Information
Symbol	Dmel\nub²	Species	D. melanogaster
Name		FlyBase ID	FBal0013179
Feature type	allele	Associated gene	Dmel\nub
Also Known As	nb²

Allele class	hypomorphic allele - genetic evidence
Mutagen	X ray
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Ng et al., 1995, Cifuentes and Garcia-Bellido, 1997)
Mutagen	X ray
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Rearrangement in the genomic DNA approximately 5kb upstream from the first exon of the β transcript. (Ng et al., 1995)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective (Endo et al., 2007) viable (Cifuentes and Garcia-Bellido, 1997) visible (Cifuentes and Garcia-Bellido, 1997, del Alamo Rodriguez et al., 2002)
Phenotype Manifest In
	adult antennal lobe projection neuron DC3 adPN (Endo et al., 2007) adult antennal lobe projection neuron VA2 adPN (Endo et al., 2007) adult antennal lobe projection neuron VM2 adPN (Endo et al., 2007) adult olfactory receptor neuron (Endo et al., 2007) adult olfactory receptor neuron Or42a (Endo et al., 2007) adult olfactory receptor neuron Or43b (Endo et al., 2007) adult olfactory receptor neuron Or83c (Endo et al., 2007) adult olfactory receptor neuron Or85a (Endo et al., 2007) adult olfactory receptor neuron Or92a (Endo et al., 2007) antennal lobe glomerulus DA4m (Endo et al., 2007) antennal lobe glomerulus DC3 (Endo et al., 2007) antennal lobe glomerulus DL2 (Endo et al., 2007) antennal lobe glomerulus DM5 (Endo et al., 2007) antennal lobe glomerulus DM6 (Endo et al., 2007) antennal lobe glomerulus DP1l (Endo et al., 2007) antennal lobe glomerulus VA1 (Endo et al., 2007) antennal lobe glomerulus VA1v (Endo et al., 2007) antennal lobe glomerulus VA2 (Endo et al., 2007) antennal lobe glomerulus VA7l (Endo et al., 2007) antennal lobe glomerulus VA7m (Endo et al., 2007) antennal lobe glomerulus VL2a (Endo et al., 2007) antennal lobe glomerulus VL2p (Endo et al., 2007) antennal lobe glomerulus VM5 (Endo et al., 2007) antennal lobe glomerulus VM5v (Endo et al., 2007) antennal lobe glomerulus VM7 (Endo et al., 2007) anterior notal wing process (Ng et al., 1995) crossvein (Cifuentes and Garcia-Bellido, 1997) dorsal triple row (Cifuentes and Garcia-Bellido, 1997) haltere (Cifuentes and Garcia-Bellido, 1997) medial triple row (Cifuentes and Garcia-Bellido, 1997) olfactory receptor neuron (Endo et al., 2007) pleural wing process (Ng et al., 1995) posterior notal wing process (Ng et al., 1995) ventral triple row (Cifuentes and Garcia-Bellido, 1997) wing (Ng et al., 1995, Cifuentes and Garcia-Bellido, 1997) wing disc (Cifuentes and Garcia-Bellido, 1997) wing hinge (del Alamo Rodriguez et al., 2002) wing vein L4 (Cifuentes and Garcia-Bellido, 1997)
Detailed Description
	Statement Reference In nub[2] mutants, the axonal projections to glomeruli VL2a, DL2d, VL2p, DP1I, VA7I, VM7c, VM7d, VM5v, VM2, VA2, DM5, VM5d, DA2, VA6, VA3, DM6, VA7m, VA1v, DA4m and DC3 are abolished while the projections to DL3, VA1d, and DL4 remain. nub[2] olfactory organ cell clones induced at 0 hours after puparium formation oly generate inner-cell clones. (Endo et al., 2007) Mutant flies lack the wing hinge. (del Alamo Rodriguez et al., 2002) Wings are smaller and shorter than normal and are abnormally folded and bent. Wing size reduction is due to reduced number of wing cells. Bristles of the triple row are incorrectly differentiated. Bracteated bristles can be seen in distal position, indicating a failure in proximo-distal specification. Wing vein L4 and crossveins are absent. Haltere is smaller than wild type. Third larval instar mutant wings exhibit conspicuous cell mortality. Clones in the wing are small and cause reduction of the whole wing size. Clones covering the proximal wing vein L2 causes thickening and those covering the triple row cause bracteated bristles. (Cifuentes and Garcia-Bellido, 1997) Wing blade reduced, wing hinge is partially deleted. Wing margin is reproducibly interrupted at the tip of the wing. Clonal analysis reveals that mutant clones are considerably smaller than wild type clones, though the autonomous reduction in size is insufficient to explain the overall reduction in size of mutant wings. Clones which occupy a portion of the wing hinge cause a non-autonomous reduction in the size of the wing. (Ng et al., 1995) Wings small and spoonlike but less extreme than nub. Patches of dried blood on wings. Veins L1 to L4 almost indiscernible; L5 and alula frequently absent. Viability and fertility excellent. RK1.
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference dpp^d12, nub² has visible phenotype (Cifuentes and Garcia-Bellido, 1997) dpp^d12/dpp^s8, nub² has visible phenotype (Cifuentes and Garcia-Bellido, 1997) dpp^s8, nub² has visible phenotype (Cifuentes and Garcia-Bellido, 1997) dpp^αTub84B.PZ, nub² has visible phenotype (Cifuentes and Garcia-Bellido, 1997) elA¹, nub² has visible phenotype (Cifuentes and Garcia-Bellido, 1997, Cifuentes and Garcia-Bellido, 1997)
Phenotype Manifest In
Enhancer of
Statement Reference nub² is an enhancer of wing disc phenotype of ft^k07918 (Garoia et al., 2000)
Other
Statement Reference dpp^d12, nub² has wing phenotype (Cifuentes and Garcia-Bellido, 1997) dpp^d12/dpp^s8, nub² has wing phenotype (Cifuentes and Garcia-Bellido, 1997) dpp^s8, nub² has wing phenotype (Cifuentes and Garcia-Bellido, 1997) dpp^αTub84B.PZ, nub² has wing phenotype (Cifuentes and Garcia-Bellido, 1997) elA¹, nub² has wing phenotype (Cifuentes and Garcia-Bellido, 1997, Cifuentes and Garcia-Bellido, 1997)
Additional Comments
Genetic Interactions
Statement Reference Double mutant clones with dpp^d12/dpp^s8 or elA¹ cause dramatic reduction of cell number in the wing pouch. Clones of dpp-expressing cells (dpp^αTub84B.PZ) cause some overgrowth but exclusively of dpp-expressing cells. (Cifuentes and Garcia-Bellido, 1997)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 6 )
Bloomington	358 w¹¹¹⁸; nub² 1298 cl¹ fy² nub² 3628 y¹ w^*; nub² b¹ sna^Sco pr¹ cn¹/CyO
Kyoto	105872 nub² 106270 cl¹ fy² nub² 107138 y¹ w^*; nub² b¹ sna^Sco pr¹ cn¹/CyO
Notes on Origin
Discoverer	R. F. Grell, 1st June 1956.

Comments
	"X ray" was stated as tentative.
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	nb² (Gibert et al., 2002, Endo et al., 2007) nub²
Name Synonym
Secondary FlyBase IDs

References ( 10 )
Research paper	Endo et al., 2007, Nat. Neurosci. 10(2): 153--160 Notch signal organizes the Drosophila olfactory circuitry by diversifying the sensory neuronal lineages. [FBrf0192533] Whitworth and Russell, 2003, Dev. Biol. 254(2): 277--288 Temporally dynamic response to Wingless directs the sequential elaboration of the proximodistal axis of the Drosophila wing. [FBrf0157154] Cohen et al., 2002, Dev. Cell 2(4): 437--448 DWnt4 regulates cell movement and focal adhesion kinase during Drosophila ovarian morphogenesis. [FBrf0146974] del Alamo Rodriguez et al., 2002, Development 129(17): 3995--4004 Different mechanisms initiate and maintain wingless expression in the Drosophila wing hinge. [FBrf0151276] Gibert et al., 2002, Dev. Genes Evol. 212(1): 19--29 Heterospecific transgenesis in Drosophila suggests that engrailed.a is regulated by POU proteins in the crustacean Sacculina carcini. [FBrf0144811] Garoia et al., 2000, Mech. Dev. 94(1-2): 95--109 Cell behaviour of Drosophila fat cadherin mutations in wing development. [FBrf0128473] Cifuentes and Garcia-Bellido, 1997, Proc. Natl. Acad. Sci. U.S.A. 94(21): 11405--11410 Proximo-distal specification in the wing disc of Drosophila by the nubbin gene. [FBrf0098758] Ng et al., 1995, Development 121(2): 589--599 nubbin encodes a POU-domain protein required for proximal-distal patterning in the Drosophila wing. [FBrf0076143] Grell and Lewis, 1956, D. I. S. 30: 71 [New mutants report.] [FBrf0010760]
Personal communication to FlyBase	Deal and Cook, 2003.4.29, Isolation and characterization of Df(2L)BSC30. Isolation and characterization of Df(2L)BSC30. [FBrf0157319]

Allele Dmel\nub2

Allele Dmel\nub²